Restriction map for
pFN21AB7007
(Linear) (Six-base) MAPSORT of: KIBB7007.seq Check: 8581 from: 1 to: 4272
KIBB7007.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 158 922 2006 2340 2655 2670 3290 4272
Size: 158 764 1084 334 315 15 620 982
Fragments arranged by size:
1084 982 764 620 334 315 158 15
AccI GT'mk_AC
Cuts at: 0 1028 2858 3626 3860 4272
Size: 1028 1830 768 234 412
Fragments arranged by size:
1830 1028 768 412 234
Acc65I G'GTAC_C
Cuts at: 0 950 4272
Size: 950 3322
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 838 1636 2507 3355 3364 3548 4272
Size: 305 533 798 871 848 9 184 724
Fragments arranged by size:
871 848 798 724 533 305 184 9
AfeI AGC'GCT
Cuts at: 0 2624 4272
Size: 2624 1648
AflIII A'CryG_T
Cuts at: 0 1172 1267 1971 2534 3196 4272
Size: 1172 95 704 563 662 1076
Fragments arranged by size:
1172 1076 704 662 563 95
AhdI GACnn_n'nnGTC
Cuts at: 0 3867 3954 4272
Size: 3867 87 318
Fragments arranged by size:
3867 318 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 157 354 2254 4033 4272
Size: 157 197 1900 1779 239
Fragments arranged by size:
1900 1779 239 197 157
AlwNI CAG_nnn'CTG
Cuts at: 0 315 399 489 567 1138 1666 2074 3481
Size: 315 84 90 78 571 528 408 1407
Cuts at: 3481 3744 4272
Size: 263 528
Fragments arranged by size:
1407 571 528 528 408 315 263 90
84 78
ApaI G_GGCC'C
Cuts at: 0 670 1716 3273 3834 4272
Size: 670 1046 1557 561 438
Fragments arranged by size:
1557 1046 670 561 438
ApaLI G'TGCA_C
Cuts at: 0 305 4272
Size: 305 3967
ApoI r'AATT_y
Cuts at: 0 365 1510 2176 2983 3358 3459 3643 4272
Size: 365 1145 666 807 375 101 184 629
Fragments arranged by size:
1145 807 666 629 375 365 184 101
AvaI C'yCGr_G
Cuts at: 0 581 1389 2586 3272 3827 4272
Size: 581 808 1197 686 555 445
Fragments arranged by size:
1197 808 686 581 555 445
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 942 975 1538 1571 4272
Size: 942 33 563 33 2701
Fragments arranged by size:
2701 942 563 33 33
BamHI G'GATC_C
Cuts at: 0 1332 4272
Size: 1332 2940
BanI G'GyrC_C
Cuts at: 0 492 648 715 950 1461 2837 4272
Size: 492 156 67 235 511 1376 1435
Fragments arranged by size:
1435 1376 511 492 235 156 67
BanII G_rGCy'C
Cuts at: 0 521 670 1366 1706 1716 1750 1893 1921
Size: 521 149 696 340 10 34 143 28
Cuts at: 1921 3273 3584 3797 3834 3980 4272
Size: 1352 311 213 37 146 292
Fragments arranged by size:
1352 696 521 340 311 292 213 149
146 143 37 34 28 10
BbeI G_GCGC'C
Cuts at: 0 719 4272
Size: 719 3553
BbsI GAAGACnn'nnnn_
Cuts at: 0 34 1079 4272
Size: 34 1045 3193
Fragments arranged by size:
3193 1045 34
BbvCI CC'TCA_GC
Cuts at: 0 392 3745 4034 4272
Size: 392 3353 289 238
Fragments arranged by size:
3353 392 289 238
BfrBI ATG'CAT
Cuts at: 0 2113 4272
Size: 2113 2159
BglI GCCn_nnn'nGGC
Cuts at: 0 665 2603 4272
Size: 665 1938 1669
Fragments arranged by size:
1938 1669 665
BglII A'GATC_T
Cuts at: 0 3104 4272
Size: 3104 1168
BlpI GC'TnA_GC
Cuts at: 0 1189 4272
Size: 1189 3083
Bme1580I G_kGCm'C
Cuts at: 0 169 309 670 1716 2840 2996 3273 3834
Size: 169 140 361 1046 1124 156 277 561
Cuts at: 3834 4272
Size: 438
Fragments arranged by size:
1124 1046 561 438 361 277 169 156
140
BmgBI CAC'GTC
Cuts at: 0 797 2435 2447 2535 4272
Size: 797 1638 12 88 1737
Fragments arranged by size:
1737 1638 797 88 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 1086 1364 4272
Size: 1086 278 2908
Fragments arranged by size:
2908 1086 278
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 314 2937 3705 3999 4272
Size: 314 2623 768 294 273
Fragments arranged by size:
2623 768 314 294 273
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 1018 1386 2141 2907 2990 3256 3274
Size: 616 402 368 755 766 83 266 18
Cuts at: 3274 4272
Size: 998
Fragments arranged by size:
998 766 755 616 402 368 266 83
18
Bpu10I CC'TnA_GC
Cuts at: 0 392 3745 3851 4034 4272
Size: 392 3353 106 183 238
Fragments arranged by size:
3353 392 238 183 106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 1935 4272
Size: 724 1211 2337
Fragments arranged by size:
2337 1211 724
BsaI GGTCTCn'nnnn_
Cuts at: 0 2508 4272
Size: 2508 1764
BsaAI yAC'GTr
Cuts at: 0 1268 4272
Size: 1268 3004
BsaBI GATnn'nnATC
Cuts at: 0 1523 4272
Size: 1523 2749
BsaHI Gr'CG_yC
Cuts at: 0 716 1019 4272
Size: 716 303 3253
Fragments arranged by size:
3253 716 303
BsaWI w'CCGG_w
Cuts at: 0 2295 4272
Size: 2295 1977
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1215 1245 2709 2739 2944 2974 4067 4097
Size: 1215 30 1464 30 205 30 1093 30
Cuts at: 4097 4272
Size: 175
Fragments arranged by size:
1464 1215 1093 205 175 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 211 240 721 979 982 1003 1374 1619
Size: 211 29 481 258 3 21 371 245
Cuts at: 1619 1774 3094 3253 3310 3328 3331 3334 3352
Size: 155 1320 159 57 18 3 3 18
Cuts at: 3352 3640 3795 3907 4272
Size: 288 155 112 365
Fragments arranged by size:
1320 481 371 365 288 258 245 211
159 155 155 112 57 29 21 18
18 3 3 3
BseYI C'CCAG_C
Cuts at: 0 28 266 1734 2577 2619 4272
Size: 28 238 1468 843 42 1653
Fragments arranged by size:
1653 1468 843 238 42 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 130 1925 1930 2445 2497 2747 2912
Size: 69 61 1795 5 515 52 250 165
Cuts at: 2912 2996 3221 3268 4272
Size: 84 225 47 1004
Fragments arranged by size:
1795 1004 515 250 225 165 84 69
61 52 47 5
BsiHKAI G_wGCw'C
Cuts at: 0 309 521 2918 3281 4272
Size: 309 212 2397 363 991
Fragments arranged by size:
2397 991 363 309 212
BsmI GAATG_Cn'
Cuts at: 0 2367 4272
Size: 2367 1905
Bsp1286I G_dGCh'C
Cuts at: 0 169 309 521 670 1366 1706 1716 1750
Size: 169 140 212 149 696 340 10 34
Cuts at: 1750 1893 1921 2840 2918 2996 3273 3281 3584
Size: 143 28 919 78 78 277 8 303
Cuts at: 3584 3797 3834 3980 4272
Size: 213 37 146 292
Fragments arranged by size:
919 696 340 303 292 277 213 212
169 149 146 143 140 78 78 37
34 28 10 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 158 922 2006 2340 2655 2670 3290 4272
Size: 158 764 1084 334 315 15 620 982
Fragments arranged by size:
1084 982 764 620 334 315 158 15
BsrDI GCAATG_nn'
Cuts at: 0 1883 2328 4272
Size: 1883 445 1944
Fragments arranged by size:
1944 1883 445
BsrFI r'CCGG_y
Cuts at: 0 277 4272
Size: 277 3995
BssSI C'ACGA_G
Cuts at: 0 336 520 4272
Size: 336 184 3752
Fragments arranged by size:
3752 336 184
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2689 2963 4272
Size: 2689 274 1309
Fragments arranged by size:
2689 1309 274
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2607 4272
Size: 2607 1665
BstYI r'GATC_y
Cuts at: 0 1332 3104 4272
Size: 1332 1772 1168
Fragments arranged by size:
1772 1332 1168
BtgI C'CryG_G
Cuts at: 0 119 185 1965 2600 4272
Size: 119 66 1780 635 1672
Fragments arranged by size:
1780 1672 635 119 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1253 4272
Size: 1253 3019
BtsI GCAGTG_nn'
Cuts at: 0 288 357 924 1942 2823 4272
Size: 288 69 567 1018 881 1449
Fragments arranged by size:
1449 1018 881 567 288 69
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 778 813 4272
Size: 778 35 3459
Fragments arranged by size:
3459 778 35
DraI TTT'AAA
Cuts at: 0 1089 4272
Size: 1089 3183
DrdI GACnn_nn'nnGTC
Cuts at: 0 1024 1288 4272
Size: 1024 264 2984
Fragments arranged by size:
2984 1024 264
EaeI y'GGCC_r
Cuts at: 0 635 1559 1902 2429 2777 4272
Size: 635 924 343 527 348 1495
Fragments arranged by size:
1495 924 635 527 348 343
EarI CTCTTCn'nnn_
Cuts at: 0 1003 2123 3329 3549 4272
Size: 1003 1120 1206 220 723
Fragments arranged by size:
1206 1120 1003 723 220
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 394 598 4272
Size: 394 204 3674
Fragments arranged by size:
3674 394 204
EcoICRI GAG'CTC
Cuts at: 0 519 4272
Size: 519 3753
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 616 838 1018 1386 1636 2141 2507
Size: 305 311 222 180 368 250 505 366
Cuts at: 2507 2907 2990 3256 3274 3355 3364 3548 4272
Size: 400 83 266 18 81 9 184 724
Fragments arranged by size:
724 505 400 368 366 311 305 266
250 222 184 180 83 81 18 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 703 724 2525 3292 3655 4272
Size: 703 21 1801 767 363 617
Fragments arranged by size:
1801 767 703 617 363 21
EcoO109I rG'GnC_Cy
Cuts at: 0 543 666 1712 1713 3269 4272
Size: 543 123 1046 1 1556 1003
Fragments arranged by size:
1556 1046 1003 543 123 1
EcoRI G'AATT_C
Cuts at: 0 2983 4272
Size: 2983 1289
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 256 1250 3072 4272
Size: 256 994 1822 1200
Fragments arranged by size:
1822 1200 994 256
FspI TGC'GCA
Cuts at: 0 976 4272
Size: 976 3296
HaeII r_GCGC'y
Cuts at: 0 719 2626 2801 4272
Size: 719 1907 175 1471
Fragments arranged by size:
1907 1471 719 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1441 1473 2742 2774 2893 2925 3091 3123
Size: 1441 32 1269 32 119 32 166 32
Cuts at: 3123 3802 3834 3853 3885 3940 3972 4067 4099
Size: 679 32 19 32 55 32 95 32
Cuts at: 4099 4208 4240 4272
Size: 109 32 32
Fragments arranged by size:
1441 1269 679 166 119 109 95 55
32 32 32 32 32 32 32 32
32 32 19
HincII GTy'rAC
Cuts at: 0 1029 1067 1118 3129 3627 3861 3906 4086
Size: 1029 38 51 2011 498 234 45 180
Cuts at: 4086 4272
Size: 186
Fragments arranged by size:
2011 1029 498 234 186 180 51 45
38
HindIII A'AGCT_T
Cuts at: 0 1853 3049 4272
Size: 1853 1196 1223
Fragments arranged by size:
1853 1223 1196
HpaI GTT'AAC
Cuts at: 0 1067 1118 4272
Size: 1067 51 3154
Fragments arranged by size:
3154 1067 51
KasI G'GCGC_C
Cuts at: 0 715 4272
Size: 715 3557
KpnI G_GTAC'C
Cuts at: 0 954 4272
Size: 954 3318
MfeI C'AATT_G
Cuts at: 0 4094 4272
Size: 4094 178
MluI A'CGCG_T
Cuts at: 0 3196 4272
Size: 3196 1076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3422 4272
Size: 3422 850
MscI TGG'CCA
Cuts at: 0 1561 1904 2431 4272
Size: 1561 343 527 1841
Fragments arranged by size:
1841 1561 527 343
MslI CAynn'nnrTG
Cuts at: 0 336 1964 1970 4272
Size: 336 1628 6 2302
Fragments arranged by size:
2302 1628 336 6
MspA1I CmG'CkG
Cuts at: 0 399 465 555 624 2843 2906 3010 3366
Size: 399 66 90 69 2219 63 104 356
Cuts at: 3366 3933 4272
Size: 567 339
Fragments arranged by size:
2219 567 399 356 339 104 90 69
66 63
NaeI GCC'GGC
Cuts at: 0 279 4272
Size: 279 3993
NarI GG'CG_CC
Cuts at: 0 716 4272
Size: 716 3556
NcoI C'CATG_G
Cuts at: 0 119 1965 4272
Size: 119 1846 2307
Fragments arranged by size:
2307 1846 119
NdeI CA'TA_TG
Cuts at: 0 3566 4272
Size: 3566 706
NgoMIV G'CCGG_C
Cuts at: 0 277 4272
Size: 277 3995
NsiI A_TGCA'T
Cuts at: 0 2115 4272
Size: 2115 2157
NspI r_CATG'y
Cuts at: 0 335 1065 1176 1197 1975 3450 4272
Size: 335 730 111 21 778 1475 822
Fragments arranged by size:
1475 822 778 730 335 111 21
PasI CC'CwG_GG
Cuts at: 0 1708 4272
Size: 1708 2564
PciI A'CATG_T
Cuts at: 0 1172 1971 4272
Size: 1172 799 2301
Fragments arranged by size:
2301 1172 799
PflMI CCAn_nnn'nTGG
Cuts at: 0 489 567 4272
Size: 489 78 3705
Fragments arranged by size:
3705 489 78
PfoI T'CCnGG_A
Cuts at: 0 3290 4272
Size: 3290 982
PmlI CAC'GTG
Cuts at: 0 1268 4272
Size: 1268 3004
PpuMI rG'GwC_Cy
Cuts at: 0 543 4272
Size: 543 3729
PshAI GACnn'nnGTC
Cuts at: 0 1247 4272
Size: 1247 3025
PsiI TTA'TAA
Cuts at: 0 2182 3163 4272
Size: 2182 981 1109
Fragments arranged by size:
2182 1109 981
PspOMI G'GGCC_C
Cuts at: 0 666 1712 3269 3830 4272
Size: 666 1046 1557 561 442
Fragments arranged by size:
1557 1046 666 561 442
PstI C_TGCA'G
Cuts at: 0 352 404 665 919 947 1249 2337 2818
Size: 352 52 261 254 28 302 1088 481
Cuts at: 2818 3618 4272
Size: 800 654
Fragments arranged by size:
1088 800 654 481 352 302 261 254
52 28
PvuII CAG'CTG
Cuts at: 0 399 555 624 2906 3010 3933 4272
Size: 399 156 69 2282 104 923 339
Fragments arranged by size:
2282 923 399 339 156 104 69
RsrII CG'GwC_CG
Cuts at: 0 381 4272
Size: 381 3891
SacI G_AGCT'C
Cuts at: 0 521 4272
Size: 521 3751
SalI G'TCGA_C
Cuts at: 0 1027 3625 3859 4272
Size: 1027 2598 234 413
Fragments arranged by size:
2598 1027 413 234
SanDI GG'GwC_CC
Cuts at: 0 543 4272
Size: 543 3729
SbfI CC_TGCA'GG
Cuts at: 0 665 4272
Size: 665 3607
ScaI AGT'ACT
Cuts at: 0 2962 4272
Size: 2962 1310
SfcI C'TryA_G
Cuts at: 0 348 400 661 915 943 1218 1245 2333
Size: 348 52 261 254 28 275 27 1088
Cuts at: 2333 2814 3614 4272
Size: 481 800 658
Fragments arranged by size:
1088 800 658 481 348 275 261 254
52 28 27
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 665 4272
Size: 665 3607
SfoI GGC'GCC
Cuts at: 0 717 4272
Size: 717 3555
SmaI CCC'GGG
Cuts at: 0 3274 3829 4272
Size: 3274 555 443
Fragments arranged by size:
3274 555 443
SmlI C'TyrA_G
Cuts at: 0 581 703 1914 4272
Size: 581 122 1211 2358
Fragments arranged by size:
2358 1211 581 122
SphI G_CATG'C
Cuts at: 0 335 1197 4272
Size: 335 862 3075
Fragments arranged by size:
3075 862 335
SspI AAT'ATT
Cuts at: 0 2267 4171 4272
Size: 2267 1904 101
Fragments arranged by size:
2267 1904 101
StuI AGG'CCT
Cuts at: 0 346 4272
Size: 346 3926
StyI C'CwwG_G
Cuts at: 0 119 128 638 753 1965 3656 3800 4272
Size: 119 9 510 115 1212 1691 144 472
Fragments arranged by size:
1691 1212 510 472 144 119 115 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 150 292 4272
Size: 150 142 3980
Fragments arranged by size:
3980 150 142
TatI w'GTAC_w
Cuts at: 0 2960 4272
Size: 2960 1312
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1215 1247 2186 2218 3541 3573 4272
Size: 1215 32 939 32 1323 32 699
Fragments arranged by size:
1323 1215 939 699 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3622 3840 4081 4272
Size: 3622 218 241 191
Fragments arranged by size:
3622 241 218 191
XhoI C'TCGA_G
Cuts at: 0 581 4272
Size: 581 3691
XmaI C'CCGG_G
Cuts at: 0 3272 3827 4272
Size: 3272 555 445
Fragments arranged by size:
3272 555 445
XmnI GAAnn'nnTTC
Cuts at: 0 784 1150 2303 3050 4272
Size: 784 366 1153 747 1222
Fragments arranged by size:
1222 1153 784 747 366
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AhdI AlfI
AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BssSI BstAPI
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PstI
PvuII RsrII SacI SalI SanDI SbfI ScaI SfcI
SfiI SfoI SmaI SmlI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XhoI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AleI AloI AscI AseI
AsiSI AvrII BcgI BciVI BclI BmtI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrGI BssHII BstBI BstEII
BstZ17I Bsu36I ClaI DraIII EagI EcoRV FseI FspAI
NheI NotI NruI PacI PmeI PpiI PspXI PsrI
PvuI SacII SapI SexAI SgfI SgrAI SnaBI SpeI
SrfI SwaI XbaI XcmI ZraI