(Circular) (Six-base) MAPSORT of: pFN21AB6004.seq Check: 2043 from: 1 to: 10639 pFN21AB6004 10639 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8449 278 Size: 53 83 186 936 6913 2468 Fragments arranged by size: 6913 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3367 7982 1937 Size: 1430 4615 4594 Fragments arranged by size: 4615 4594 1430 Acc65I G'GTAC_C Cuts at: 7960 7960 Size: 10639 AclI AA'CG_TT Cuts at: 1563 8766 9139 1563 Size: 7203 373 3063 Fragments arranged by size: 7203 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4092 5051 5075 5132 5541 5760 5814 7032 Size: 2460 959 24 57 409 219 54 1218 Cuts at: 7032 7290 7707 7731 7941 8702 10086 10354 1632 Size: 258 417 24 210 761 1384 268 1917 Fragments arranged by size: 2460 1917 1384 1218 959 761 417 409 268 258 219 210 57 54 24 24 AfeI AGC'GCT Cuts at: 3877 3877 Size: 10639 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10442 AflIII A'CryG_T Cuts at: 1203 3091 3546 5468 7853 9538 10515 1203 Size: 1888 455 1922 2385 1685 977 1327 Fragments arranged by size: 2385 1922 1888 1685 1327 977 455 AgeI A'CCGG_T Cuts at: 9470 9470 Size: 10639 AhdI GACnn_n'nnGTC Cuts at: 2912 2973 3949 9368 2912 Size: 61 976 5419 4183 Fragments arranged by size: 5419 4183 976 61 AleI CACnn'nnGTG Cuts at: 1166 3283 1166 Size: 2117 8522 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 7256 1184 Size: 6072 4567 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7008 7040 7008 Size: 32 10607 AlwNI CAG_nnn'CTG Cuts at: 5354 7010 8382 9954 5354 Size: 1656 1372 1572 6039 Fragments arranged by size: 6039 1656 1572 1372 ApaLI G'TGCA_C Cuts at: 8696 9852 8696 Size: 1156 9483 ApoI r'AATT_y Cuts at: 1493 2154 2700 2726 3576 3773 4163 4877 5535 Size: 661 546 26 850 197 390 714 658 Cuts at: 5535 5718 5862 5945 6311 6368 6623 6916 6922 Size: 183 144 83 366 57 255 293 6 Cuts at: 6922 7877 7948 8174 8326 10435 1493 Size: 955 71 226 152 2109 1697 Fragments arranged by size: 2109 1697 955 850 714 661 658 546 390 366 293 255 226 197 183 152 144 83 71 57 26 6 AseI AT'TA_AT Cuts at: 160 1032 2519 3026 3075 6947 9193 160 Size: 872 1487 507 49 3872 2246 1606 Fragments arranged by size: 3872 2246 1606 1487 872 507 49 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10639 AvaI C'yCGr_G Cuts at: 1870 1943 2028 7964 1870 Size: 73 85 5936 4545 Fragments arranged by size: 5936 4545 85 73 AvrII C'CTAG_G Cuts at: 3482 3482 Size: 10639 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5838 5871 5838 Size: 33 10606 BamHI G'GATC_C Cuts at: 3054 7969 8336 3054 Size: 4915 367 5357 Fragments arranged by size: 5357 4915 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2553 7180 7960 9415 618 Size: 325 217 627 766 4627 780 1455 1842 Fragments arranged by size: 4627 1842 1455 780 766 627 325 217 BanII G_rGCy'C Cuts at: 1391 7958 1391 Size: 6567 4072 BbeI G_GCGC'C Cuts at: 2557 2557 Size: 10639 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2466 2808 3466 5888 7194 7287 7712 Size: 971 567 342 658 2422 1306 93 425 Cuts at: 7712 7719 928 Size: 7 3848 Fragments arranged by size: 3848 2422 1306 971 658 567 425 342 93 7 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5579 5613 8830 8864 1903 Size: 34 3642 34 3217 34 3678 Fragments arranged by size: 3678 3642 3217 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3396 8531 9741 3396 Size: 5135 1210 4294 Fragments arranged by size: 5135 4294 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 10639 BfrBI ATG'CAT Cuts at: 3541 3541 Size: 10639 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 9250 136 Size: 107 122 71 631 605 7578 1525 Fragments arranged by size: 7578 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7021 7516 10634 7021 Size: 495 3118 7026 Fragments arranged by size: 7026 3118 495 BlpI GC'TnA_GC Cuts at: 6537 8056 6537 Size: 1519 9120 Bme1580I G_kGCm'C Cuts at: 1790 6270 7185 8700 9856 1790 Size: 4480 915 1515 1156 2573 Fragments arranged by size: 4480 2573 1515 1156 915 BmgBI CAC'GTC Cuts at: 1318 7856 1318 Size: 6538 4101 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3471 4554 4889 Size: 416 53 83 399 24 2054 1083 335 Cuts at: 4889 5880 8370 9328 442 Size: 991 2490 958 1753 Fragments arranged by size: 2490 2054 1753 1083 991 958 416 399 335 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10639 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2440 2997 2440 Size: 557 10082 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2070 3974 9299 1239 Size: 831 1904 5325 2579 Fragments arranged by size: 5325 2579 1904 831 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2410 3490 5496 8764 9629 9927 2410 Size: 1080 2006 3268 865 298 3122 Fragments arranged by size: 3268 3122 2006 1080 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2005 2123 3156 3531 9302 882 Size: 617 506 118 1033 375 5771 2219 Fragments arranged by size: 5771 2219 1033 617 506 375 118 BsaAI yAC'GTr Cuts at: 493 1204 2150 2684 8430 493 Size: 711 946 534 5746 2702 Fragments arranged by size: 5746 2702 946 711 534 BsaBI GATnn'nnATC Cuts at: 3396 3604 3610 3616 3894 4911 10633 3396 Size: 208 6 6 278 1017 5722 3402 Fragments arranged by size: 5722 3402 1017 278 208 6 6 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2554 7739 8446 8828 Size: 53 83 186 936 1021 5185 707 382 Cuts at: 8828 9463 275 Size: 635 1451 Fragments arranged by size: 5185 1451 1021 936 707 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5053 9072 9470 9744 9891 10302 5053 Size: 4019 398 274 147 411 5390 Fragments arranged by size: 5390 4019 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2134 2164 5508 5538 6751 Size: 30 165 30 731 30 3344 30 1213 Cuts at: 6751 6781 7008 7038 7131 7161 1178 Size: 30 227 30 93 30 4656 Fragments arranged by size: 4656 3344 1213 731 227 165 93 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2046 5534 5606 5951 6414 6608 6759 7016 Size: 860 3488 72 345 463 194 151 257 Cuts at: 7016 7364 1186 Size: 348 4461 Fragments arranged by size: 4461 3488 860 463 348 345 257 194 151 72 BseYI C'CCAG_C Cuts at: 2134 5402 9842 10338 2134 Size: 3268 4440 496 2435 Fragments arranged by size: 4440 3268 2435 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 6822 1163 Size: 573 5086 4980 Fragments arranged by size: 5086 4980 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8089 8850 9878 1540 Size: 275 186 6088 761 1028 2301 Fragments arranged by size: 6088 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2062 2149 8700 8785 9856 2062 Size: 87 6551 85 1071 2845 Fragments arranged by size: 6551 2845 1071 87 85 BsmI GAATG_Cn' Cuts at: 2544 5406 8150 8243 2544 Size: 2862 2744 93 4940 Fragments arranged by size: 4940 2862 2744 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2062 2149 6270 7185 7958 8700 8785 Size: 399 272 87 4121 915 773 742 85 Cuts at: 8785 9856 1391 Size: 1071 2174 Fragments arranged by size: 4121 2174 1071 915 773 742 399 272 87 85 BspEI T'CCGG_A Cuts at: 10302 10302 Size: 10639 BspHI T'CATG_A Cuts at: 2272 6501 8528 10258 2272 Size: 4229 2027 1730 2653 Fragments arranged by size: 4229 2653 2027 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4895 5240 7994 844 Size: 4051 345 2754 3489 Fragments arranged by size: 4051 3489 2754 345 BsrBI CCG'CTC Cuts at: 1394 1823 3052 8526 1394 Size: 429 1229 5474 3507 Fragments arranged by size: 5474 3507 1229 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4016 6054 7836 9134 9308 65 Size: 1183 2768 2038 1782 1298 174 1396 Fragments arranged by size: 2768 2038 1782 1396 1298 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2310 9283 9470 1700 Size: 110 500 6973 187 2869 Fragments arranged by size: 6973 2869 500 187 110 BsrGI T'GTAC_A Cuts at: 96 5035 96 Size: 4939 5700 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10639 BssSI C'ACGA_G Cuts at: 2055 6771 8699 9711 2055 Size: 4716 1928 1012 2983 Fragments arranged by size: 4716 2983 1928 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4181 794 Size: 3387 7252 BstBI TT'CG_AA Cuts at: 9443 9443 Size: 10639 BstEII G'GTnAC_C Cuts at: 1184 5216 1184 Size: 4032 6607 BstYI r'GATC_y Cuts at: 1972 2108 2668 3054 3405 3605 3611 3617 5971 Size: 136 560 386 351 200 6 6 2354 Cuts at: 5971 6414 7021 7516 7969 8336 8721 8738 10190 Size: 443 607 495 453 367 385 17 1452 Cuts at: 10190 10276 10288 10634 1972 Size: 86 12 346 1977 Fragments arranged by size: 2354 1977 1452 607 560 495 453 443 386 385 367 351 346 200 136 86 17 12 6 6 Bsu36I CC'TnA_GG Cuts at: 6210 7752 7776 6210 Size: 1542 24 9073 Fragments arranged by size: 9073 1542 24 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 10087 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9900 Fragments arranged by size: 9900 683 56 BtsI GCAGTG_nn' Cuts at: 2355 3850 6412 7240 7840 8155 8949 8969 2355 Size: 1495 2562 828 600 315 794 20 4025 Fragments arranged by size: 4025 2562 1495 828 794 600 315 20 ClaI AT'CG_AT Cuts at: 1554 2631 5029 1554 Size: 1077 2398 7164 Fragments arranged by size: 7164 2398 1077 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10604 DraI TTT'AAA Cuts at: 4168 4800 5604 7119 7374 7943 4168 Size: 632 804 1515 255 569 6864 Fragments arranged by size: 6864 1515 804 632 569 255 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3375 4297 9646 809 Size: 507 2059 922 5349 1802 Fragments arranged by size: 5349 2059 1802 922 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2173 7884 8086 Size: 54 1425 50 275 18 343 5711 202 Cuts at: 8086 8975 10604 8 Size: 889 1629 43 Fragments arranged by size: 5711 1629 1425 889 343 275 202 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8086 1537 Size: 275 6274 4090 Fragments arranged by size: 6274 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3212 5430 6208 6816 7068 7266 7308 7430 Size: 1859 2218 778 608 252 198 42 122 Cuts at: 7430 7626 7723 7814 8569 1353 Size: 196 97 91 755 3423 Fragments arranged by size: 3423 2218 1859 778 755 608 252 198 196 122 97 91 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7307 9226 9600 9746 387 Size: 1064 138 5718 1919 374 146 1280 Fragments arranged by size: 5718 1919 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2070 3974 4092 5051 5075 5132 5541 Size: 393 438 1904 118 959 24 57 409 Cuts at: 5541 5760 5814 7032 7290 7707 7731 7941 8702 Size: 219 54 1218 258 417 24 210 761 Cuts at: 8702 9299 10086 10354 1239 Size: 597 787 268 1524 Fragments arranged by size: 1904 1524 1218 959 787 761 597 438 417 409 393 268 258 219 210 118 57 54 24 24 EcoNI CCTnn'n_nnAGG Cuts at: 4746 7031 4746 Size: 2285 8354 EcoRI G'AATT_C Cuts at: 2154 5718 5862 7948 8326 2154 Size: 3564 144 2086 378 4467 Fragments arranged by size: 4467 3564 2086 378 144 EcoRV GAT'ATC Cuts at: 4807 4807 Size: 10639 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2615 2690 4825 5081 5272 6452 7334 2615 Size: 75 2135 256 191 1180 882 5920 Fragments arranged by size: 5920 2135 1180 882 256 191 75 FspI TGC'GCA Cuts at: 9145 9145 Size: 10639 HaeII r_GCGC'y Cuts at: 1678 2557 3879 9786 1678 Size: 879 1322 5907 2531 Fragments arranged by size: 5907 2531 1322 879 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2068 2100 2898 2930 2959 Size: 32 135 32 496 32 798 32 29 Cuts at: 2959 2991 3319 3351 3680 3712 3935 3967 5348 Size: 32 328 32 329 32 223 32 1381 Cuts at: 5348 5380 5508 5540 6483 6515 7268 7300 7649 Size: 32 128 32 943 32 753 32 349 Cuts at: 7649 7681 7840 7872 9280 9312 9354 9386 1373 Size: 32 159 32 1408 32 42 32 2626 Fragments arranged by size: 2626 1408 1381 943 798 753 496 349 329 328 223 159 135 128 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 29 HincII GTy'rAC Cuts at: 669 1657 1938 2938 3368 3380 5229 7983 8229 Size: 988 281 1000 430 12 1849 2754 246 Cuts at: 8229 669 Size: 3079 Fragments arranged by size: 3079 2754 1849 1000 988 430 281 246 12 HindIII A'AGCT_T Cuts at: 748 3998 4488 6434 748 Size: 3250 490 1946 4953 Fragments arranged by size: 4953 3250 1946 490 HpaI GTT'AAC Cuts at: 8229 8229 Size: 10639 KasI G'GCGC_C Cuts at: 2553 2553 Size: 10639 KpnI G_GTAC'C Cuts at: 7964 7964 Size: 10639 MfeI C'AATT_G Cuts at: 5325 8238 5325 Size: 2913 7726 MluI A'CGCG_T Cuts at: 10515 10515 Size: 10639 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2729 3000 3076 3251 5065 7324 7583 9753 Size: 1424 271 76 175 1814 2259 259 2170 Cuts at: 9753 9937 1305 Size: 184 2007 Fragments arranged by size: 2259 2170 2007 1814 1424 271 259 184 175 76 MscI TGG'CCA Cuts at: 10 64 1489 1832 2175 7886 10 Size: 54 1425 343 343 5711 2763 Fragments arranged by size: 5711 2763 1425 343 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3201 3283 8597 8956 9115 518 Size: 648 2035 82 5314 359 159 2042 Fragments arranged by size: 5314 2042 2035 648 359 159 82 MspA1I CmG'CkG Cuts at: 1606 6836 8055 8732 9880 10125 1606 Size: 5230 1219 677 1148 245 2120 Fragments arranged by size: 5230 2120 1219 1148 677 245 NarI GG'CG_CC Cuts at: 2554 2554 Size: 10639 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 10087 NdeI CA'TA_TG Cuts at: 387 5223 387 Size: 4836 5803 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10639 NotI GC'GGCC_GC Cuts at: 8086 8086 Size: 10639 NsiI A_TGCA'T Cuts at: 3543 3543 Size: 10639 NspI r_CATG'y Cuts at: 1132 1229 2645 3095 3200 3550 5472 7997 9542 Size: 97 1416 450 105 350 1922 2525 1545 Cuts at: 9542 1132 Size: 2229 Fragments arranged by size: 2525 2229 1922 1545 1416 450 350 105 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 10639 PciI A'CATG_T Cuts at: 3091 3546 5468 9538 3091 Size: 455 1922 4070 4192 Fragments arranged by size: 4192 4070 1922 455 PflMI CCAn_nnn'nTGG Cuts at: 1108 4061 4793 1108 Size: 2953 732 6954 Fragments arranged by size: 6954 2953 732 PfoI T'CCnGG_A Cuts at: 2086 4249 2086 Size: 2163 8476 PmeI GTTT'AAAC Cuts at: 7943 7943 Size: 10639 PmlI CAC'GTG Cuts at: 2150 2150 Size: 10639 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7008 7040 8708 8740 10221 10253 7008 Size: 32 1668 32 1481 32 7394 Fragments arranged by size: 7394 1668 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10639 PsiI TTA'TAA Cuts at: 4043 4976 8209 4043 Size: 933 3233 6473 Fragments arranged by size: 6473 3233 933 PspXI vC'TCGA_Gb Cuts at: 2028 2028 Size: 10639 PstI C_TGCA'G Cuts at: 830 6067 7991 830 Size: 5237 1924 3478 Fragments arranged by size: 5237 3478 1924 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10639 PvuII CAG'CTG Cuts at: 6836 6836 Size: 10639 SalI G'TCGA_C Cuts at: 1936 3366 7981 1936 Size: 1430 4615 4594 Fragments arranged by size: 4615 4594 1430 SapI GCTCTTCn'nnn_ Cuts at: 5430 7068 7308 5430 Size: 1638 240 8761 Fragments arranged by size: 8761 1638 240 SbfI CC_TGCA'GG Cuts at: 7991 7991 Size: 10639 ScaI AGT'ACT Cuts at: 5398 8887 10599 5398 Size: 3489 1712 5438 Fragments arranged by size: 5438 3489 1712 SfcI C'TryA_G Cuts at: 826 1045 4407 4428 4758 5787 6063 6222 7209 Size: 219 3362 21 330 1029 276 159 987 Cuts at: 7209 7987 9122 9803 9994 826 Size: 778 1135 681 191 1471 Fragments arranged by size: 3362 1471 1135 1029 987 778 681 330 276 219 191 159 21 SfoI GGC'GCC Cuts at: 2555 2555 Size: 10639 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10639 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10639 SmaI CCC'GGG Cuts at: 1872 7966 1872 Size: 6094 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2028 2425 3505 5511 8743 9644 Size: 197 926 85 397 1080 2006 3232 901 Cuts at: 9644 9906 820 Size: 262 1553 Fragments arranged by size: 3232 2006 1553 1080 926 901 397 262 197 85 SnaBI TAC'GTA Cuts at: 493 2684 8430 493 Size: 2191 5746 2702 Fragments arranged by size: 5746 2702 2191 SpeI A'CTAG_T Cuts at: 152 6131 6140 152 Size: 5979 9 4651 Fragments arranged by size: 5979 4651 9 SphI G_CATG'C Cuts at: 1132 2645 7997 1132 Size: 1513 5352 3774 Fragments arranged by size: 5352 3774 1513 SspI AAT'ATT Cuts at: 5 52 3273 5212 5826 5902 8563 5 Size: 47 3221 1939 614 76 2661 2081 Fragments arranged by size: 3221 2661 2081 1939 614 76 47 StuI AGG'CCT Cuts at: 1517 2083 2104 7637 1517 Size: 566 21 5533 4519 Fragments arranged by size: 5533 4519 566 21 StyI C'CwwG_G Cuts at: 513 1065 3482 4398 4980 6306 8078 513 Size: 552 2417 916 582 1326 1772 3074 Fragments arranged by size: 3074 2417 1772 1326 916 582 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2838 3138 3513 8666 8683 8836 9021 Size: 1255 1258 300 375 5153 17 153 185 Cuts at: 9021 9466 325 Size: 445 1498 Fragments arranged by size: 5153 1498 1258 1255 445 375 300 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2395 3748 4067 Size: 274 80 33 51 1443 418 1353 319 Cuts at: 4067 4194 5035 5074 5396 7850 8885 10597 96 Size: 127 841 39 322 2454 1035 1712 138 Fragments arranged by size: 2454 1712 1443 1353 1035 841 418 322 319 274 138 127 80 51 39 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2134 2166 2653 2685 4218 4250 6538 6570 10599 Size: 32 487 32 1533 32 2288 32 4029 Cuts at: 10599 10631 2134 Size: 32 2142 Fragments arranged by size: 4029 2288 2142 1533 487 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 10639 XbaI T'CTAG_A Cuts at: 3491 3596 4528 5722 7975 3491 Size: 105 932 1194 2253 6155 Fragments arranged by size: 6155 2253 1194 932 105 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2260 6346 2260 Size: 4086 6553 XhoI C'TCGA_G Cuts at: 1943 2028 1943 Size: 85 10554 XmaI C'CCGG_G Cuts at: 1870 7964 1870 Size: 6094 4545 XmnI GAAnn'nnTTC Cuts at: 3463 5535 8768 3463 Size: 2072 3233 5334 Fragments arranged by size: 5334 3233 2072 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8447 276 Size: 53 83 186 936 6913 2468 Fragments arranged by size: 6913 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I FseI FspAI NaeI NgoMIV NruI PacI PpuMI PspOMI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI