(Circular) (Six-base) MAPSORT of: pFN21AB6002.seq Check: 51 from: 1 to: 9763 pFN21AB6002 9763 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2617 2617 Size: 9763 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7573 278 Size: 53 83 186 936 6037 2468 Fragments arranged by size: 6037 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6888 7106 1937 Size: 4951 218 4594 Fragments arranged by size: 4951 4594 218 Acc65I G'GTAC_C Cuts at: 7084 7084 Size: 9763 AclI AA'CG_TT Cuts at: 1563 4699 7890 8263 1563 Size: 3136 3191 373 3063 Fragments arranged by size: 3191 3136 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3517 5323 5673 5688 6006 6643 6645 6783 Size: 1885 1806 350 15 318 637 2 138 Cuts at: 6783 7826 9210 9478 1632 Size: 1043 1384 268 1917 Fragments arranged by size: 1917 1885 1806 1384 1043 637 350 318 268 138 15 2 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9566 AflIII A'CryG_T Cuts at: 1203 3323 4412 5089 5793 8662 9639 1203 Size: 2120 1089 677 704 2869 977 1327 Fragments arranged by size: 2869 2120 1327 1089 977 704 677 AgeI A'CCGG_T Cuts at: 8594 8594 Size: 9763 AhdI GACnn_n'nnGTC Cuts at: 8492 8492 Size: 9763 AleI CACnn'nnGTG Cuts at: 1166 5372 1166 Size: 4206 5557 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9763 AlwNI CAG_nnn'CTG Cuts at: 7506 9078 7506 Size: 1572 8191 ApaLI G'TGCA_C Cuts at: 7820 8976 7820 Size: 1156 8607 ApoI r'AATT_y Cuts at: 1493 2708 2804 3185 3985 4089 4204 4302 4456 Size: 1215 96 381 800 104 115 98 154 Cuts at: 4456 4850 4959 6345 6398 6843 6967 7072 7298 Size: 394 109 1386 53 445 124 105 226 Cuts at: 7298 7450 9559 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1386 1215 800 445 394 381 226 154 152 124 115 109 105 104 98 96 53 AseI AT'TA_AT Cuts at: 160 1032 5403 5901 8317 160 Size: 872 4371 498 2416 1606 Fragments arranged by size: 4371 2416 1606 872 498 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9763 AvaI C'yCGr_G Cuts at: 1870 1943 7088 1870 Size: 73 5145 4545 Fragments arranged by size: 5145 4545 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3590 3623 3590 Size: 33 9730 BamHI G'GATC_C Cuts at: 2824 2908 5615 7093 7460 2824 Size: 84 2707 1478 367 5127 Fragments arranged by size: 5127 2707 1478 367 84 BanI G'GyrC_C Cuts at: 618 943 1160 1787 7084 8539 618 Size: 325 217 627 5297 1455 1842 Fragments arranged by size: 5297 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2378 3905 5866 6225 7082 1391 Size: 987 1527 1961 359 857 4072 Fragments arranged by size: 4072 1961 1527 987 857 359 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2561 3185 3882 4721 6197 6373 928 Size: 971 662 624 697 839 1476 176 4318 Fragments arranged by size: 4318 1476 971 839 697 662 624 176 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3767 3801 7954 7988 1903 Size: 34 1830 34 4153 34 3678 Fragments arranged by size: 4153 3678 1830 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7655 8865 7655 Size: 1210 8553 BclI T'GATC_A Cuts at: 1548 4858 1548 Size: 3310 6453 BfrBI ATG'CAT Cuts at: 6742 6742 Size: 9763 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 8374 136 Size: 107 122 71 631 605 6702 1525 Fragments arranged by size: 6702 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4921 5428 5478 5500 6751 9758 4921 Size: 507 50 22 1251 3007 4926 Fragments arranged by size: 4926 3007 1251 507 50 22 BlpI GC'TnA_GC Cuts at: 6186 7180 6186 Size: 994 8769 Bme1580I G_kGCm'C Cuts at: 1790 4015 7824 8980 1790 Size: 2225 3809 1156 2573 Fragments arranged by size: 3809 2573 2225 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9763 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 7494 8452 442 Size: 416 53 83 399 24 6077 958 1753 Fragments arranged by size: 6077 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2903 1056 Size: 1847 7916 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4046 6654 8423 1239 Size: 2807 2608 1769 2579 Fragments arranged by size: 2807 2608 2579 1769 Bpu10I CC'TnA_GC Cuts at: 5648 5648 Size: 9763 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2324 4545 5273 7033 7888 8753 9051 2324 Size: 2221 728 1760 855 865 298 3036 Fragments arranged by size: 3036 2221 1760 865 855 728 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3286 8426 882 Size: 617 1787 5140 2219 Fragments arranged by size: 5140 2219 1787 617 BsaAI yAC'GTr Cuts at: 493 1204 3324 7554 493 Size: 711 2120 4230 2702 Fragments arranged by size: 4230 2702 2120 711 BsaBI GATnn'nnATC Cuts at: 2223 3672 6743 9757 2223 Size: 1449 3071 3014 2229 Fragments arranged by size: 3071 3014 2229 1449 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7570 7952 8587 275 Size: 53 83 186 936 6037 382 635 1451 Fragments arranged by size: 6037 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2056 8196 8594 8868 9015 9426 2056 Size: 6140 398 274 147 411 2393 Fragments arranged by size: 6140 2393 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 6675 6705 1178 Size: 30 165 30 5272 30 4236 Fragments arranged by size: 5272 4236 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3155 3885 4548 5190 6699 1186 Size: 1969 730 663 642 1509 4250 Fragments arranged by size: 4250 1969 1509 730 663 642 BseYI C'CCAG_C Cuts at: 3372 8966 9462 3372 Size: 5594 496 3673 Fragments arranged by size: 5594 3673 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 6969 1163 Size: 573 5233 3957 Fragments arranged by size: 5233 3957 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7213 7974 9002 1540 Size: 275 186 5212 761 1028 2301 Fragments arranged by size: 5212 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5104 7824 7909 8980 5104 Size: 2720 85 1071 5887 Fragments arranged by size: 5887 2720 1071 85 BsmI GAATG_Cn' Cuts at: 2504 2535 5383 6556 7274 7367 2504 Size: 31 2848 1173 718 93 4900 Fragments arranged by size: 4900 2848 1173 718 93 31 BsmBI CGTCTCn'nnnn_ Cuts at: 5285 7016 5285 Size: 1731 8032 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2378 3905 4015 5104 5866 6225 7082 Size: 399 588 1527 110 1089 762 359 857 Cuts at: 7082 7824 7909 8980 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1527 1089 1071 857 762 742 588 399 359 110 85 BspEI T'CCGG_A Cuts at: 2056 9426 2056 Size: 7370 2393 BspHI T'CATG_A Cuts at: 3472 7652 9382 3472 Size: 4180 1730 3853 Fragments arranged by size: 4180 3853 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2617 6641 7118 844 Size: 1773 4024 477 3489 Fragments arranged by size: 4024 3489 1773 477 BsrBI CCG'CTC Cuts at: 1394 1823 7650 1394 Size: 429 5827 3507 Fragments arranged by size: 5827 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3457 3546 4790 6147 8258 8432 65 Size: 1183 2209 89 1244 1357 2111 174 1396 Fragments arranged by size: 2209 2111 1396 1357 1244 1183 174 89 BsrFI r'CCGG_y Cuts at: 1700 1810 8407 8594 1700 Size: 110 6597 187 2869 Fragments arranged by size: 6597 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4939 5065 5161 96 Size: 4843 126 96 4698 Fragments arranged by size: 4843 4698 126 96 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9763 BssSI C'ACGA_G Cuts at: 7823 8835 7823 Size: 1012 8751 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9763 BstBI TT'CG_AA Cuts at: 3028 5779 8567 3028 Size: 2751 2788 4224 Fragments arranged by size: 4224 2788 2751 BstEII G'GTnAC_C Cuts at: 1184 6027 1184 Size: 4843 4920 BstXI CCAn_nnnn'nTGG Cuts at: 5005 5005 Size: 9763 BstYI r'GATC_y Cuts at: 1972 2824 2908 4159 4871 4921 5428 5478 5500 Size: 852 84 1251 712 50 507 50 22 Cuts at: 5500 5615 6751 7093 7460 7845 7862 9314 9400 Size: 115 1136 342 367 385 17 1452 86 Cuts at: 9400 9412 9758 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1251 1136 852 712 507 385 367 346 342 115 86 84 50 50 22 17 12 BstZ17I GTA'TAC Cuts at: 6889 6889 Size: 9763 Bsu36I CC'TnA_GG Cuts at: 2841 5582 2841 Size: 2741 7022 BtgI C'CryG_G Cuts at: 513 1065 2004 3585 513 Size: 552 939 1581 6691 Fragments arranged by size: 6691 1581 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9024 Fragments arranged by size: 9024 683 56 BtsI GCAGTG_nn' Cuts at: 4324 7279 8073 8093 4324 Size: 2955 794 20 5994 Fragments arranged by size: 5994 2955 794 20 ClaI AT'CG_AT Cuts at: 1554 2274 1554 Size: 720 9043 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2061 2096 5283 5318 529 Size: 35 1497 35 3187 35 4974 Fragments arranged by size: 4974 3187 1497 35 35 35 DraI TTT'AAA Cuts at: 2035 2715 3021 3594 4168 4308 4399 5623 7067 Size: 680 306 573 574 140 91 1224 1444 Cuts at: 7067 2035 Size: 4731 Fragments arranged by size: 4731 1444 1224 680 574 573 306 140 91 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6267 8770 809 Size: 507 4951 2503 1802 Fragments arranged by size: 4951 2503 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2429 7210 8099 Size: 54 1425 50 275 18 599 4781 889 Cuts at: 8099 9728 8 Size: 1629 43 Fragments arranged by size: 4781 1629 1425 889 599 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7210 1537 Size: 275 5398 4090 Fragments arranged by size: 5398 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2871 2928 3117 3702 7693 1353 Size: 1518 57 189 585 3991 3423 Fragments arranged by size: 3991 3423 1518 585 189 57 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8350 8724 8870 387 Size: 1064 138 6761 374 146 1280 Fragments arranged by size: 6761 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3517 4046 5323 5673 5688 6006 6643 Size: 393 1885 529 1277 350 15 318 637 Cuts at: 6643 6645 6654 6783 7826 8423 9210 9478 1239 Size: 2 9 129 1043 597 787 268 1524 Fragments arranged by size: 1885 1524 1277 1043 787 637 597 529 393 350 318 268 129 15 9 2 EcoNI CCTnn'n_nnAGG Cuts at: 6669 6669 Size: 9763 EcoRI G'AATT_C Cuts at: 7072 7450 7072 Size: 378 9385 EcoRV GAT'ATC Cuts at: 2016 2272 2797 4257 2016 Size: 256 525 1460 7522 Fragments arranged by size: 7522 1460 525 256 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2318 3033 4225 4643 6181 2318 Size: 715 1192 418 1538 5900 Fragments arranged by size: 5900 1538 1192 715 418 FspI TGC'GCA Cuts at: 8269 8269 Size: 9763 HaeII r_GCGC'y Cuts at: 1678 4752 8910 1678 Size: 3074 4158 2531 Fragments arranged by size: 4158 3074 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2140 2172 3507 3539 8404 Size: 32 135 32 568 32 1335 32 4865 Cuts at: 8404 8436 8478 8510 1373 Size: 32 42 32 2626 Fragments arranged by size: 4865 2626 1335 568 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4567 7107 7353 669 Size: 988 281 2629 2540 246 3079 Fragments arranged by size: 3079 2629 2540 988 281 246 HindIII A'AGCT_T Cuts at: 748 2635 4362 4680 4944 748 Size: 1887 1727 318 264 5567 Fragments arranged by size: 5567 1887 1727 318 264 HpaI GTT'AAC Cuts at: 7353 7353 Size: 9763 KpnI G_GTAC'C Cuts at: 7088 7088 Size: 9763 MfeI C'AATT_G Cuts at: 4262 7362 4262 Size: 3100 6663 MluI A'CGCG_T Cuts at: 9639 9639 Size: 9763 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6890 8877 9061 1305 Size: 5585 1987 184 2007 Fragments arranged by size: 5585 2007 1987 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2431 10 Size: 54 1425 343 599 7342 Fragments arranged by size: 7342 1425 599 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3356 5372 6734 6737 7721 8080 8239 Size: 648 2190 2016 1362 3 984 359 159 Cuts at: 8239 518 Size: 2042 Fragments arranged by size: 2190 2042 2016 1362 984 648 359 159 3 MspA1I CmG'CkG Cuts at: 1606 2028 7042 7179 7856 9004 9249 1606 Size: 422 5014 137 677 1148 245 2120 Fragments arranged by size: 5014 2120 1148 677 422 245 137 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 8272 Fragments arranged by size: 8272 939 552 NdeI CA'TA_TG Cuts at: 387 3254 5893 6787 387 Size: 2867 2639 894 3363 Fragments arranged by size: 3363 2867 2639 894 NheI G'CTAG_C Cuts at: 1052 2899 1052 Size: 1847 7916 NotI GC'GGCC_GC Cuts at: 7210 7210 Size: 9763 NsiI A_TGCA'T Cuts at: 6744 6744 Size: 9763 NspI r_CATG'y Cuts at: 1132 1229 4416 5093 5797 5831 7121 8666 1132 Size: 97 3187 677 704 34 1290 1545 2229 Fragments arranged by size: 3187 2229 1545 1290 704 677 97 34 PasI CC'CwG_GG Cuts at: 1348 3052 1348 Size: 1704 8059 PciI A'CATG_T Cuts at: 4412 5089 5793 8662 4412 Size: 677 704 2869 5513 Fragments arranged by size: 5513 2869 704 677 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 9763 PfoI T'CCnGG_A Cuts at: 2572 4465 2572 Size: 1893 7870 PmeI GTTT'AAAC Cuts at: 7067 7067 Size: 9763 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7832 7864 9345 9377 7832 Size: 32 1481 32 8218 Fragments arranged by size: 8218 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9763 PsiI TTA'TAA Cuts at: 2208 3356 7333 2208 Size: 1148 3977 4638 Fragments arranged by size: 4638 3977 1148 PstI C_TGCA'G Cuts at: 830 3450 3955 6646 7115 830 Size: 2620 505 2691 469 3478 Fragments arranged by size: 3478 2691 2620 505 469 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9763 PvuII CAG'CTG Cuts at: 7042 7042 Size: 9763 SalI G'TCGA_C Cuts at: 1936 7105 1936 Size: 5169 4594 SapI GCTCTTCn'nnn_ Cuts at: 3702 3702 Size: 9763 SbfI CC_TGCA'GG Cuts at: 7115 7115 Size: 9763 ScaI AGT'ACT Cuts at: 2665 8011 9723 2665 Size: 5346 1712 2705 Fragments arranged by size: 5346 2705 1712 SfcI C'TryA_G Cuts at: 826 1045 2547 2771 3342 3446 3757 3951 4002 Size: 219 1502 224 571 104 311 194 51 Cuts at: 4002 4511 6090 6642 7111 8246 8927 9118 826 Size: 509 1579 552 469 1135 681 191 1471 Fragments arranged by size: 1579 1502 1471 1135 681 571 552 509 469 311 224 219 194 191 104 51 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9763 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9763 SmaI CCC'GGG Cuts at: 1872 7090 1872 Size: 5218 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2303 4560 5288 7012 7867 8768 Size: 197 926 360 2257 728 1724 855 901 Cuts at: 8768 9030 820 Size: 262 1553 Fragments arranged by size: 2257 1724 1553 926 901 855 728 360 262 197 SnaBI TAC'GTA Cuts at: 493 7554 493 Size: 7061 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9763 SphI G_CATG'C Cuts at: 1132 7121 1132 Size: 5989 3774 SspI AAT'ATT Cuts at: 5 52 3025 4740 5320 7687 5 Size: 47 2973 1715 580 2367 2081 Fragments arranged by size: 2973 2367 2081 1715 580 47 StuI AGG'CCT Cuts at: 1517 5960 1517 Size: 4443 5320 StyI C'CwwG_G Cuts at: 513 1065 2004 2912 3463 3833 7202 513 Size: 552 939 908 551 370 3369 3074 Fragments arranged by size: 3369 3074 939 908 552 551 370 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2306 4734 5087 6968 7790 7807 7960 Size: 1255 726 2428 353 1881 822 17 153 Cuts at: 7960 8145 8590 325 Size: 185 445 1498 Fragments arranged by size: 2428 1881 1498 1255 822 726 445 353 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2663 2699 3130 Size: 274 80 33 51 1443 686 36 431 Cuts at: 3130 4939 5065 5161 5248 6587 6940 8009 9721 Size: 1809 126 96 87 1339 353 1069 1712 Cuts at: 9721 96 Size: 138 Fragments arranged by size: 1809 1712 1443 1339 1069 686 431 353 274 138 126 96 87 80 51 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6675 6707 6809 6841 9723 9755 6675 Size: 32 102 32 2882 32 6683 Fragments arranged by size: 6683 2882 102 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9763 XbaI T'CTAG_A Cuts at: 7099 7099 Size: 9763 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3986 4761 5600 5640 3986 Size: 775 839 40 8109 Fragments arranged by size: 8109 839 775 40 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9763 XmaI C'CCGG_G Cuts at: 1870 7088 1870 Size: 5218 4545 XmnI GAAnn'nnTTC Cuts at: 3300 4208 4278 5139 5430 6971 7004 7892 3300 Size: 908 70 861 291 1541 33 888 5171 Fragments arranged by size: 5171 1541 908 888 861 291 70 33 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7571 276 Size: 53 83 186 936 6037 2468 Fragments arranged by size: 6037 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI BsiWI DraIII EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI