Restriction map for
pFN21AB5651
(Circular) (Six-base) MAPSORT of: pFN21AB5651.seq Check: 8646 from: 1 to: 5905
pFN21AB5651 5905 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3715 278
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2791 3248 1937
Size: 854 457 4594
Fragments arranged by size:
4594 854 457
Acc65I G'GTAC_C
Cuts at: 3226 3226
Size: 5905
AclI AA'CG_TT
Cuts at: 1563 3108 4032 4405 1563
Size: 1545 924 373 3063
Fragments arranged by size:
3063 1545 924 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2699 3153 3968 5352 5620 1632
Size: 1067 454 815 1384 268 1917
Fragments arranged by size:
1917 1384 1067 815 454 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5708
AflIII A'CryG_T
Cuts at: 1203 4804 5781 1203
Size: 3601 977 1327
Fragments arranged by size:
3601 1327 977
AgeI A'CCGG_T
Cuts at: 4736 4736
Size: 5905
AhdI GACnn_n'nnGTC
Cuts at: 4634 4634
Size: 5905
AleI CACnn'nnGTG
Cuts at: 1166 2531 1166
Size: 1365 4540
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2796 1184
Size: 1612 4293
AlwNI CAG_nnn'CTG
Cuts at: 2491 2707 3648 5220 2491
Size: 216 941 1572 3176
Fragments arranged by size:
3176 1572 941 216
ApaLI G'TGCA_C
Cuts at: 3962 5118 3962
Size: 1156 4749
ApoI r'AATT_y
Cuts at: 1493 3214 3440 3592 5701 1493
Size: 1721 226 152 2109 1697
Fragments arranged by size:
2109 1721 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4459 160
Size: 872 3427 1606
Fragments arranged by size:
3427 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
AvaI C'yCGr_G
Cuts at: 1870 1943 2224 2386 3230 1870
Size: 73 281 162 844 4545
Fragments arranged by size:
4545 844 281 162 73
AvrII C'CTAG_G
Cuts at: 2468 2468
Size: 5905
BamHI G'GATC_C
Cuts at: 3235 3602 3235
Size: 367 5538
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2020 2246 2294 3226 4681
Size: 325 217 627 233 226 48 932 1455
Cuts at: 4681 618
Size: 1842
Fragments arranged by size:
1842 1455 932 627 325 233 226 217
48
BanII G_rGCy'C
Cuts at: 1391 2183 2658 2766 3189 3224 1391
Size: 792 475 108 423 35 4072
Fragments arranged by size:
4072 792 475 423 108 35
BbeI G_GCGC'C
Cuts at: 2024 2024
Size: 5905
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3086 3141 928
Size: 971 1187 55 3692
Fragments arranged by size:
3692 1187 971 55
BbvCI CC'TCA_GC
Cuts at: 2996 2996
Size: 5905
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4096 4130 1903
Size: 34 2159 34 3678
Fragments arranged by size:
3678 2159 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2566 3010 3797 5007 2566
Size: 444 787 1210 3464
Fragments arranged by size:
3464 1210 787 444
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5905
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2044 4516 136
Size: 107 122 71 631 605 372 2472 1525
Fragments arranged by size:
2472 1525 631 605 372 122 107 71
BglII A'GATC_T
Cuts at: 5900 5900
Size: 5905
BlpI GC'TnA_GC
Cuts at: 3322 3322
Size: 5905
Bme1580I G_kGCm'C
Cuts at: 1790 2251 2297 3966 5122 1790
Size: 461 46 1669 1156 2573
Fragments arranged by size:
2573 1669 1156 461 46
BmgBI CAC'GTC
Cuts at: 1318 2062 2362 1318
Size: 744 300 4861
Fragments arranged by size:
4861 744 300
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2601 2819 3636
Size: 416 53 83 399 24 1184 218 817
Cuts at: 3636 4594 442
Size: 958 1753
Fragments arranged by size:
1753 1184 958 817 416 399 218 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4565 1239
Size: 3326 2579
Bpu10I CC'TnA_GC
Cuts at: 2615 2996 2615
Size: 381 5524
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4030 4895 5193 4030
Size: 865 298 4742
Fragments arranged by size:
4742 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4568 882
Size: 617 3069 2219
Fragments arranged by size:
3069 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3696 493
Size: 711 2492 2702
Fragments arranged by size:
2702 2492 711
BsaBI GATnn'nnATC
Cuts at: 5899 5899
Size: 5905
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2021 3712 4094 4729
Size: 53 83 186 936 488 1691 382 635
Cuts at: 4729 275
Size: 1451
Fragments arranged by size:
1691 1451 936 635 488 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2983 4338 4736 5010 5157 5568 2983
Size: 1355 398 274 147 411 3320
Fragments arranged by size:
3320 1355 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5680
Fragments arranged by size:
5680 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2774 1186
Size: 1588 4317
BseYI C'CCAG_C
Cuts at: 2240 2250 2290 2391 2559 5108 5604 2240
Size: 10 40 101 168 2549 496 2541
Fragments arranged by size:
2549 2541 496 168 101 40 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5332
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3355 4116 5144 1540
Size: 275 186 1354 761 1028 2301
Fragments arranged by size:
2301 1354 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2766 2939 3966 4051 5122 2766
Size: 173 1027 85 1071 3549
Fragments arranged by size:
3549 1071 1027 173 85
BsmI GAATG_Cn'
Cuts at: 2754 3416 3509 2754
Size: 662 93 5150
Fragments arranged by size:
5150 662 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2183 2251 2297 2658 2766 2939 3189
Size: 399 393 68 46 361 108 173 250
Cuts at: 3189 3224 3966 4051 5122 1391
Size: 35 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 393 361 250 173
108 85 68 46 35
BspEI T'CCGG_A
Cuts at: 2983 5568 2983
Size: 2585 3320
BspHI T'CATG_A
Cuts at: 2109 2658 2766 3794 5524 2109
Size: 549 108 1028 1730 2490
Fragments arranged by size:
2490 1730 1028 549 108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3260 844
Size: 2416 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2032 2262 3792 1394
Size: 429 209 230 1530 3507
Fragments arranged by size:
3507 1530 429 230 209
BsrDI GCAATG_nn'
Cuts at: 65 1248 3007 4400 4574 65
Size: 1183 1759 1393 174 1396
Fragments arranged by size:
1759 1396 1393 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2297 4549 4736 1700
Size: 110 487 2252 187 2869
Fragments arranged by size:
2869 2252 487 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5905
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5905
BssSI C'ACGA_G
Cuts at: 2128 2570 3965 4977 2128
Size: 442 1395 1012 3056
Fragments arranged by size:
3056 1395 1012 442
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2707 794
Size: 1913 3992
BstBI TT'CG_AA
Cuts at: 2746 4709 2746
Size: 1963 3942
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5905
BstYI r'GATC_y
Cuts at: 1972 2142 2870 2893 3235 3602 3987 4004 5456
Size: 170 728 23 342 367 385 17 1452
Cuts at: 5456 5542 5554 5900 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 728 385 367 346 342 170
86 23 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2547 2802 513
Size: 552 1482 255 3616
Fragments arranged by size:
3616 1482 552 255
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2492 487
Size: 683 56 1266 3900
Fragments arranged by size:
3900 1266 683 56
BtsI GCAGTG_nn'
Cuts at: 2378 3421 4215 4235 2378
Size: 1043 794 20 4048
Fragments arranged by size:
4048 1043 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5870
DraI TTT'AAA
Cuts at: 3209 3209
Size: 5905
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4912 809
Size: 507 3596 1802
Fragments arranged by size:
3596 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2218 2409 2416
Size: 54 1425 50 275 18 388 191 7
Cuts at: 2416 3352 4241 5870 8
Size: 936 889 1629 43
Fragments arranged by size:
1629 1425 936 889 388 275 191 54
50 43 18 7
EagI C'GGCC_G
Cuts at: 1537 1812 3352 1537
Size: 275 1540 4090
Fragments arranged by size:
4090 1540 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2102 3155 3835 1353
Size: 749 1053 680 3423
Fragments arranged by size:
3423 1053 749 680
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2149 2333 4492 4866 5012 387
Size: 1064 138 560 184 2159 374 146 1280
Fragments arranged by size:
2159 1280 1064 560 374 184 146 138
EcoICRI GAG'CTC
Cuts at: 2764 2764
Size: 5905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2699 3153 3968 4565 5352 5620 1239
Size: 393 1067 454 815 597 787 268 1524
Fragments arranged by size:
1524 1067 815 787 597 454 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2071 2228 2071
Size: 157 5748
EcoRI G'AATT_C
Cuts at: 3214 3592 3214
Size: 378 5527
FspI TGC'GCA
Cuts at: 4411 4411
Size: 5905
HaeII r_GCGC'y
Cuts at: 1678 2024 5052 1678
Size: 346 3028 2531
Fragments arranged by size:
3028 2531 346
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2165 2197 2855 2887 3149
Size: 32 135 32 593 32 658 32 262
Cuts at: 3149 3181 4546 4578 4620 4652 1373
Size: 32 1365 32 42 32 2626
Fragments arranged by size:
2626 1365 658 593 262 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2792 2842 3112 3249 3495 669
Size: 988 281 854 50 270 137 246 3079
Fragments arranged by size:
3079 988 854 281 270 246 137 50
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5905
HpaI GTT'AAC
Cuts at: 3495 3495
Size: 5905
KasI G'GCGC_C
Cuts at: 2020 2020
Size: 5905
KpnI G_GTAC'C
Cuts at: 3230 3230
Size: 5905
MfeI C'AATT_G
Cuts at: 3504 3504
Size: 5905
MluI A'CGCG_T
Cuts at: 5781 5781
Size: 5905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5019 5203 1305
Size: 3714 184 2007
Fragments arranged by size:
3714 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2418 10
Size: 54 1425 343 586 3497
Fragments arranged by size:
3497 1425 586 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2531 2663 3863 4222 4381 518
Size: 648 1365 132 1200 359 159 2042
Fragments arranged by size:
2042 1365 1200 648 359 159 132
MspA1I CmG'CkG
Cuts at: 1606 2290 3321 3998 5146 5391 1606
Size: 684 1031 677 1148 245 2120
Fragments arranged by size:
2120 1148 1031 684 677 245
NarI GG'CG_CC
Cuts at: 2021 2021
Size: 5905
NcoI C'CATG_G
Cuts at: 513 1065 2547 2802 513
Size: 552 1482 255 3616
Fragments arranged by size:
3616 1482 552 255
NdeI CA'TA_TG
Cuts at: 387 2499 387
Size: 2112 3793
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5905
NotI GC'GGCC_GC
Cuts at: 3352 3352
Size: 5905
NspI r_CATG'y
Cuts at: 1132 1229 2258 3263 4808 1132
Size: 97 1029 1005 1545 2229
Fragments arranged by size:
2229 1545 1029 1005 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5905
PciI A'CATG_T
Cuts at: 4804 4804
Size: 5905
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5905
PfoI T'CCnGG_A
Cuts at: 3097 3097
Size: 5905
PmeI GTTT'AAAC
Cuts at: 3209 3209
Size: 5905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3974 4006 5487 5519 3974
Size: 32 1481 32 4360
Fragments arranged by size:
4360 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2071 2071
Size: 5905
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5905
PsiI TTA'TAA
Cuts at: 3475 3475
Size: 5905
PstI C_TGCA'G
Cuts at: 830 2289 3257 830
Size: 1459 968 3478
Fragments arranged by size:
3478 1459 968
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5905
PvuII CAG'CTG
Cuts at: 2290 2290
Size: 5905
SacI G_AGCT'C
Cuts at: 2766 2766
Size: 5905
SalI G'TCGA_C
Cuts at: 1936 2790 3247 1936
Size: 854 457 4594
Fragments arranged by size:
4594 854 457
SbfI CC_TGCA'GG
Cuts at: 3257 3257
Size: 5905
ScaI AGT'ACT
Cuts at: 4153 5865 4153
Size: 1712 4193
SfcI C'TryA_G
Cuts at: 826 1045 2285 2965 3253 4388 5069 5260 826
Size: 219 1240 680 288 1135 681 191 1471
Fragments arranged by size:
1471 1240 1135 681 680 288 219 191
SfoI GGC'GCC
Cuts at: 2022 2022
Size: 5905
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
SgrAI Cr'CCGG_yG
Cuts at: 1700 2297 1700
Size: 597 5308
SmaI CCC'GGG
Cuts at: 1872 2226 2388 3232 1872
Size: 354 162 844 4545
Fragments arranged by size:
4545 844 354 162
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4009 4910 5172 820
Size: 197 926 2066 901 262 1553
Fragments arranged by size:
2066 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3696 493
Size: 3203 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5905
SphI G_CATG'C
Cuts at: 1132 3263 1132
Size: 2131 3774
SspI AAT'ATT
Cuts at: 5 52 3829 5
Size: 47 3777 2081
Fragments arranged by size:
3777 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5905
StyI C'CwwG_G
Cuts at: 513 1065 2468 2547 2802 3344 513
Size: 552 1403 79 255 542 3074
Fragments arranged by size:
3074 1403 552 542 255 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2381 2863 3058 3932 3949 4102 4287
Size: 1255 801 482 195 874 17 153 185
Cuts at: 4287 4732 325
Size: 445 1498
Fragments arranged by size:
1498 1255 874 801 482 445 195 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4151 5863 96
Size: 274 80 33 51 1443 2174 1712 138
Fragments arranged by size:
2174 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5865 5897 5865
Size: 32 5873
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5905
XbaI T'CTAG_A
Cuts at: 3241 3241
Size: 5905
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5905
XmaI C'CCGG_G
Cuts at: 1870 2224 2386 3230 1870
Size: 354 162 844 4545
Fragments arranged by size:
4545 844 354 162
XmnI GAAnn'nnTTC
Cuts at: 2742 4034 2742
Size: 1292 4613
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3713 276
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SrfI SwaI XcmI