Restriction map for
pFN21AB8765
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I AlwI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspHI AviII AvrII BalI BamHI
BbrPI BbsI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstYI BstZ17I
CelII Cfr10I ClaI CpoI DpnII
DraI DraIII DrdI DsaI EaeI
EagI EclXI Eco47III Eco52I EcoNI
EcoRV EcoT22I FauI FbaI FseI
FspI HindIII HpaI KpnI KspI
MaeI MaeII MamI MboI MfeI
MflI MluI MluNI MroI MscI
MseI MunI NaeI NcoI NdeII
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII Sau3AI ScaI SexAI
SfaNI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
SwaI Tru9I Van91I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccI 181 GTCGAC GT^[AC][GT]AC
AccII 178 CGCG CG^CG
AciI 189 CCGC CCGC(-3/-1)
AcsI 94 GAATTC [GA]^AATT[CT]
AfaI 219 GTAC GT^AC
AhdI 48 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 427 CCTGAGG CC^T[ACGT]AGG
ApaI 323 GGGCCC GGGCC^C
ApoI 94 GAATTC [GA]^AATT[CT]
Asp700I 621 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 48 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 30 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 63 CCCGAG C^[CT]CG[GA]G
BanI 299 GGCGCC G^G[CT][GA]CC
BbeI 299 GGCGCC GGCGC^C
BbvCI 294 GCTGAGG CCTCAGC(-5/-2)
BglI 395 GCCTCAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsoBI 63 CCCGAG C^[CT]CG[GA]G
BspMI 381 GCAGGT ACCTGC(4/8)
BstEII 27 GGTGACC G^GT[ACGT]ACC
BstF5I 341 CATCC GGATG(2/0)
BstPI 27 GGTGACC G^GT[ACGT]ACC
BstUI 178 CGCG CG^CG
BstXI 140 CCAGCAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 427 CCTGAGG CC^T[ACGT]AGG
Cac8I 313 GCGTGC GC[ACGT]^[ACGT]GC
Eam1105I 48 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 508 GAAGAG CTCTTC(1/4)
Eco81I 427 CCTGAGG CC^T[ACGT]AGG
EcoO65I 27 GGTGACC G^GT[ACGT]ACC
EcoRI 94 GAATTC G^AATTC
EcoT14I 455 CCAAGG C^C[AT][AT]GG
FokI 341 CATCC GGATG(9/13)
HaeII 299 GGCGCC [GA]GCGC^[CT]
HincII 181 GTCGAC GT[CT]^[GA]AC
HindII 181 GTCGAC GT[CT]^[GA]AC
KasI 299 GGCGCC G^GCGCC
Ksp632I 508 GAAGAG CTCTTC(1/4)
MslI 341 CATCCGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 487 CAGCTG C[AC]G^C[GT]G
MvnI 178 CGCG CG^CG
NarI 299 GGCGCC GG^CGCC
NdeI 373 CATATG CA^TATG
NspI 236 GCATGT [GA]CATG^[CT]
PflFI 30 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 499 GACTC GAGTC(4/5)
PstI 286 CTGCAG CTGCA^G
PvuII 487 CAGCTG CAG^CTG
RsaI 219 GTAC GT^AC
SalI 181 GTCGAC G^TCGAC
SapI 508 GAAGAGC GCTCTTC(1/4)
SbfI 285 CCTGCAGG CCTGCA^GG
SfcI 286 CTGCAG C^T[GA][CT]AG
SfoI 299 GGCGCC GGC^GCC
SmlI 397 CTCAAG C^T[CT][GA]AG
Sse8387I 285 CCTGCAGG CCTGCA^GG
StuI 602 AGGCCT AGG^CCT
StyI 455 CCAAGG C^C[AT][AT]GG
Tsp509I 95 AATT ^AATT
Tth111I 30 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 99 CCATTTCCAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 621 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 299 GGCGCC G[GA]^CG[CT]C
438 GACGCC
AlwNI 289 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
631 CAGGCCCTG
AvaII 361 GGACC G^G[AT]CC
565 GGACC
BanII 323 GGGCCC G[GA]GC[CT]^C
412 GAGCCC
BcnI 327 CCCGG CC^[GC]GG
574 CCCGG
BmyI 323 GGGCCC G[AGT]GC[ACT]^C
412 GAGCCC
BpmI 110 CTGGAG CTGGAG(16/14)
161 CTGGAG
BsaHI 299 GGCGCC G[GA]^CG[CT]C
438 GACGCC
BsgI 126 CTGCAC GTGCAG(16/14)
348 GTGCAG
Bsp1286I 323 GGGCCC G[AGT]GC[ACT]^C
412 GAGCCC
CfoI 300 GCGC GCG^C
641 GCGC
HapII 328 CCGG C^CGG
575 CCGG
HhaI 300 GCGC GCG^C
641 GCGC
Hin1I 299 GGCGCC G[GA]^CG[CT]C
438 GACGCC
HinP1I 300 GCGC G^CGC
641 GCGC
HpaII 328 CCGG C^CGG
575 CCGG
MaeIII 28 GTGAC ^GT[ACGT]AC
385 GTGAC
MspI 328 CCGG C^CGG
575 CCGG
NciI 327 CCCGG CC^[GC]GG
574 CCCGG
NlaIII 237 CATG CATG^
310 CATG
TaqI 182 TCGA T^CGA
502 TCGA
TfiI 121 GATTC G^A[AT]TC
276 GAATC
Tsp45I 28 GTGAC ^GT[GC]AC
385 GTGAC
TspRI 84 CACTG CA[GC]TG[ACGT][ACGT]^
464 CAGTG
TthHB8I 182 TCGA T^CGA
502 TCGA
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AluI BbvI BsaJI BsiYI BslI
BsmFI BsrI BssKI BstNI Cfr13I
DdeI DraII EcoO109I EcoRII Fnu4HI
HaeIII HgaI HinfI HphI ItaI
MboII MnlI MvaI MwoI NlaIV
Sau96I ScrFI TseI