(Circular) (Six-base) MAPSORT of: pFN21AB8765.seq Check: 6814 from: 1 to: 5374 pFN21AB8765 5374 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2377 2377 Size: 5374 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3184 278 Size: 53 83 186 936 1648 2468 Fragments arranged by size: 2468 1648 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2187 2717 1937 Size: 250 530 4594 Fragments arranged by size: 4594 530 250 Acc65I G'GTAC_C Cuts at: 2695 2695 Size: 5374 AclI AA'CG_TT Cuts at: 1563 3501 3874 1563 Size: 1938 373 3063 Fragments arranged by size: 3063 1938 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3437 4821 5089 1632 Size: 1805 1384 268 1917 Fragments arranged by size: 1917 1805 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5177 AflIII A'CryG_T Cuts at: 1203 4273 5250 1203 Size: 3070 977 1327 Fragments arranged by size: 3070 1327 977 AgeI A'CCGG_T Cuts at: 4205 4205 Size: 5374 AhdI GACnn_n'nnGTC Cuts at: 2058 4103 2058 Size: 2045 3329 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5374 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5374 AlwNI CAG_nnn'CTG Cuts at: 2299 2641 3117 4689 2299 Size: 342 476 1572 2984 Fragments arranged by size: 2984 1572 476 342 ApaI G_GGCC'C Cuts at: 2332 2332 Size: 5374 ApaLI G'TGCA_C Cuts at: 3431 4587 3431 Size: 1156 4218 ApoI r'AATT_y Cuts at: 1493 2099 2683 2909 3061 5170 1493 Size: 606 584 226 152 2109 1697 Fragments arranged by size: 2109 1697 606 584 226 152 AseI AT'TA_AT Cuts at: 160 1032 3928 160 Size: 872 2896 1606 Fragments arranged by size: 2896 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 AvaI C'yCGr_G Cuts at: 1870 1943 2068 2699 1870 Size: 73 125 631 4545 Fragments arranged by size: 4545 631 125 73 BamHI G'GATC_C Cuts at: 2704 3071 2704 Size: 367 5007 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2304 2695 4150 618 Size: 325 217 627 517 391 1455 1842 Fragments arranged by size: 1842 1455 627 517 391 325 217 BanII G_rGCy'C Cuts at: 1391 2332 2421 2693 1391 Size: 941 89 272 4072 Fragments arranged by size: 4072 941 272 89 BbeI G_GCGC'C Cuts at: 2308 2308 Size: 5374 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4403 BbvCI CC'TCA_GC Cuts at: 2300 2300 Size: 5374 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3565 3599 1903 Size: 34 1628 34 3678 Fragments arranged by size: 3678 1628 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3266 4476 3266 Size: 1210 4164 BclI T'GATC_A Cuts at: 1548 1548 Size: 5374 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2406 3985 136 Size: 107 122 71 631 605 734 1579 1525 Fragments arranged by size: 1579 1525 734 631 605 122 107 71 BglII A'GATC_T Cuts at: 5369 5369 Size: 5374 BlpI GC'TnA_GC Cuts at: 2791 2791 Size: 5374 Bme1580I G_kGCm'C Cuts at: 1790 2332 3435 4591 1790 Size: 542 1103 1156 2573 Fragments arranged by size: 2573 1156 1103 542 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5374 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2100 3105 4063 Size: 416 53 83 399 24 683 1005 958 Cuts at: 4063 442 Size: 1753 Fragments arranged by size: 1753 1005 958 683 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5374 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2136 2187 4034 1239 Size: 897 51 1847 2579 Fragments arranged by size: 2579 1847 897 51 Bpu10I CC'TnA_GC Cuts at: 2300 2339 2300 Size: 39 5335 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2387 3499 4364 4662 2387 Size: 1112 865 298 3099 Fragments arranged by size: 3099 1112 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4037 882 Size: 617 2538 2219 Fragments arranged by size: 2538 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3165 493 Size: 711 1961 2702 Fragments arranged by size: 2702 1961 711 BsaBI GATnn'nnATC Cuts at: 5368 5368 Size: 5374 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2305 2444 3181 3563 Size: 53 83 186 936 772 139 737 382 Cuts at: 3563 4198 275 Size: 635 1451 Fragments arranged by size: 1451 936 772 737 635 382 186 139 83 53 BsaWI w'CCGG_w Cuts at: 3807 4205 4479 4626 5037 3807 Size: 398 274 147 411 4144 Fragments arranged by size: 4144 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5149 Fragments arranged by size: 5149 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5374 BseYI C'CCAG_C Cuts at: 2144 2237 2438 2494 2518 2562 4577 5073 2144 Size: 93 201 56 24 44 2015 496 2445 Fragments arranged by size: 2445 2015 496 201 93 56 44 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2116 2374 1163 Size: 573 380 258 4163 Fragments arranged by size: 4163 573 380 258 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2824 3585 4613 1540 Size: 275 186 823 761 1028 2301 Fragments arranged by size: 2301 1028 823 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3435 3520 4591 3435 Size: 85 1071 4218 Fragments arranged by size: 4218 1071 85 BsmI GAATG_Cn' Cuts at: 2885 2978 2885 Size: 93 5281 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2332 2421 2693 3435 3520 4591 1391 Size: 399 542 89 272 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 542 399 272 89 85 BspEI T'CCGG_A Cuts at: 5037 5037 Size: 5374 BspHI T'CATG_A Cuts at: 3263 4993 3263 Size: 1730 3644 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2377 2729 844 Size: 1533 352 3489 Fragments arranged by size: 3489 1533 352 BsrBI CCG'CTC Cuts at: 1394 1823 3261 1394 Size: 429 1438 3507 Fragments arranged by size: 3507 1438 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3869 4043 65 Size: 1183 2621 174 1396 Fragments arranged by size: 2621 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4018 4205 1700 Size: 110 2208 187 2869 Fragments arranged by size: 2869 2208 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5374 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5374 BssSI C'ACGA_G Cuts at: 3434 4446 3434 Size: 1012 4362 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5374 BstBI TT'CG_AA Cuts at: 4178 4178 Size: 5374 BstEII G'GTnAC_C Cuts at: 1184 2032 1184 Size: 848 4526 BstXI CCAn_nnnn'nTGG Cuts at: 2152 2152 Size: 5374 BstYI r'GATC_y Cuts at: 1972 2704 3071 3456 3473 4925 5011 5023 5369 Size: 732 367 385 17 1452 86 12 346 Cuts at: 5369 1972 Size: 1977 Fragments arranged by size: 1977 1452 732 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2433 2433 Size: 5374 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4822 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4635 Fragments arranged by size: 4635 683 56 BtsI GCAGTG_nn' Cuts at: 2890 3684 3704 2890 Size: 794 20 4560 Fragments arranged by size: 4560 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5374 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5339 DraI TTT'AAA Cuts at: 2678 2678 Size: 5374 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4381 809 Size: 507 3065 1802 Fragments arranged by size: 3065 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2821 3710 5339 Size: 54 1425 50 275 18 991 889 1629 Cuts at: 5339 8 Size: 43 Fragments arranged by size: 1629 1425 991 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2821 1537 Size: 275 1009 4090 Fragments arranged by size: 4090 1009 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2508 3304 1353 Size: 1155 796 3423 Fragments arranged by size: 3423 1155 796 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3961 4335 4481 387 Size: 1064 138 2372 374 146 1280 Fragments arranged by size: 2372 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2136 2187 3437 4034 4821 5089 1239 Size: 393 504 51 1250 597 787 268 1524 Fragments arranged by size: 1524 1250 787 597 504 393 268 51 EcoO109I rG'GnC_Cy Cuts at: 2065 2328 2329 2638 2065 Size: 263 1 309 4801 Fragments arranged by size: 4801 309 263 1 EcoRI G'AATT_C Cuts at: 2099 2683 3061 2099 Size: 584 378 4412 Fragments arranged by size: 4412 584 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2195 2195 Size: 5374 FspI TGC'GCA Cuts at: 3880 3880 Size: 5374 HaeII r_GCGC'y Cuts at: 1678 2308 4521 1678 Size: 630 2213 2531 Fragments arranged by size: 2531 2213 630 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2044 2076 2255 2287 2434 Size: 32 135 32 472 32 179 32 147 Cuts at: 2434 2466 4015 4047 4089 4121 1373 Size: 32 1549 32 42 32 2626 Fragments arranged by size: 2626 1549 472 179 147 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2188 2718 2964 669 Size: 988 281 250 530 246 3079 Fragments arranged by size: 3079 988 530 281 250 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5374 HpaI GTT'AAC Cuts at: 2964 2964 Size: 5374 KasI G'GCGC_C Cuts at: 2304 2304 Size: 5374 KpnI G_GTAC'C Cuts at: 2699 2699 Size: 5374 MfeI C'AATT_G Cuts at: 2973 2973 Size: 5374 MluI A'CGCG_T Cuts at: 5250 5250 Size: 5374 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2603 4488 4672 1305 Size: 1298 1885 184 2007 Fragments arranged by size: 2007 1885 1298 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3552 Fragments arranged by size: 3552 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2350 3332 3691 3850 518 Size: 648 1184 982 359 159 2042 Fragments arranged by size: 2042 1184 982 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2494 2790 3467 4615 4860 1606 Size: 888 296 677 1148 245 2120 Fragments arranged by size: 2120 1148 888 677 296 245 NarI GG'CG_CC Cuts at: 2305 2305 Size: 5374 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4822 NdeI CA'TA_TG Cuts at: 387 2379 387 Size: 1992 3382 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5374 NotI GC'GGCC_GC Cuts at: 2821 2821 Size: 5374 NspI r_CATG'y Cuts at: 1132 1229 2245 2732 4277 1132 Size: 97 1016 487 1545 2229 Fragments arranged by size: 2229 1545 1016 487 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5374 PciI A'CATG_T Cuts at: 4273 4273 Size: 5374 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5374 PmeI GTTT'AAAC Cuts at: 2678 2678 Size: 5374 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3443 3475 4956 4988 3443 Size: 32 1481 32 3829 Fragments arranged by size: 3829 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5374 PsiI TTA'TAA Cuts at: 2944 2944 Size: 5374 PspOMI G'GGCC_C Cuts at: 2328 2328 Size: 5374 PstI C_TGCA'G Cuts at: 830 2295 2726 830 Size: 1465 431 3478 Fragments arranged by size: 3478 1465 431 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5374 PvuII CAG'CTG Cuts at: 2494 2494 Size: 5374 SalI G'TCGA_C Cuts at: 1936 2186 2716 1936 Size: 250 530 4594 Fragments arranged by size: 4594 530 250 SapI GCTCTTCn'nnn_ Cuts at: 2508 2508 Size: 5374 SbfI CC_TGCA'GG Cuts at: 2295 2726 2295 Size: 431 4943 ScaI AGT'ACT Cuts at: 3622 5334 3622 Size: 1712 3662 SfcI C'TryA_G Cuts at: 826 1045 2291 2722 3857 4538 4729 826 Size: 219 1246 431 1135 681 191 1471 Fragments arranged by size: 1471 1246 1135 681 431 219 191 SfoI GGC'GCC Cuts at: 2306 2306 Size: 5374 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5374 SmaI CCC'GGG Cuts at: 1872 2701 1872 Size: 829 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2402 3478 4379 4641 820 Size: 197 926 459 1076 901 262 1553 Fragments arranged by size: 1553 1076 926 901 459 262 197 SnaBI TAC'GTA Cuts at: 493 3165 493 Size: 2672 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5374 SphI G_CATG'C Cuts at: 1132 2732 1132 Size: 1600 3774 SspI AAT'ATT Cuts at: 5 52 3298 5 Size: 47 3246 2081 Fragments arranged by size: 3246 2081 47 StuI AGG'CCT Cuts at: 1517 2609 1517 Size: 1092 4282 StyI C'CwwG_G Cuts at: 513 1065 2460 2813 513 Size: 552 1395 353 3074 Fragments arranged by size: 3074 1395 552 353 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3401 3418 3571 3756 4201 325 Size: 1255 1821 17 153 185 445 1498 Fragments arranged by size: 1821 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2223 3620 5332 Size: 274 80 33 51 1443 246 1397 1712 Cuts at: 5332 96 Size: 138 Fragments arranged by size: 1712 1443 1397 274 246 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2125 2157 5334 5366 2125 Size: 32 3177 32 2133 Fragments arranged by size: 3177 2133 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2038 1316 Size: 722 4652 XbaI T'CTAG_A Cuts at: 2710 2710 Size: 5374 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2111 2111 Size: 5374 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5374 XmaI C'CCGG_G Cuts at: 1870 2699 1870 Size: 829 4545 XmnI GAAnn'nnTTC Cuts at: 2630 3503 2630 Size: 873 4501 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3182 276 Size: 53 83 186 936 1648 2468 Fragments arranged by size: 2468 1648 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI