Restriction map for
pFN21AB8765
(Linear) (Six-base) MAPSORT of: KIBB8765.seq Check: 9286 from: 1 to: 669
KIBB8765.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 372 669
Size: 372 297
AccI GT'mk_AC
Cuts at: 0 182 669
Size: 182 487
AhdI GACnn_n'nnGTC
Cuts at: 0 53 669
Size: 53 616
AlwNI CAG_nnn'CTG
Cuts at: 0 294 636 669
Size: 294 342 33
Fragments arranged by size:
342 294 33
ApaI G_GGCC'C
Cuts at: 0 327 669
Size: 327 342
ApoI r'AATT_y
Cuts at: 0 94 669
Size: 94 575
AvaI C'yCGr_G
Cuts at: 0 63 669
Size: 63 606
BanI G'GyrC_C
Cuts at: 0 299 669
Size: 299 370
BanII G_rGCy'C
Cuts at: 0 327 416 669
Size: 327 89 253
Fragments arranged by size:
327 253 89
BbeI G_GCGC'C
Cuts at: 0 303 669
Size: 303 366
BbvCI CC'TCA_GC
Cuts at: 0 295 669
Size: 295 374
BglI GCCn_nnn'nGGC
Cuts at: 0 401 669
Size: 401 268
Bme1580I G_kGCm'C
Cuts at: 0 327 669
Size: 327 342
BmrI ACTGGGnnnn_n'
Cuts at: 0 95 669
Size: 95 574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 182 669
Size: 131 51 487
Fragments arranged by size:
487 131 51
Bpu10I CC'TnA_GC
Cuts at: 0 295 334 669
Size: 295 39 335
Fragments arranged by size:
335 295 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 669
Size: 382 287
BsaHI Gr'CG_yC
Cuts at: 0 300 439 669
Size: 300 139 230
Fragments arranged by size:
300 230 139
BseYI C'CCAG_C
Cuts at: 0 139 232 433 489 513 557 669
Size: 139 93 201 56 24 44 112
Fragments arranged by size:
201 139 112 93 56 44 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 369 669
Size: 111 258 300
Fragments arranged by size:
300 258 111
Bsp1286I G_dGCh'C
Cuts at: 0 327 416 669
Size: 327 89 253
Fragments arranged by size:
327 253 89
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 372 669
Size: 372 297
BstEII G'GTnAC_C
Cuts at: 0 27 669
Size: 27 642
BstXI CCAn_nnnn'nTGG
Cuts at: 0 147 669
Size: 147 522
Bsu36I CC'TnA_GG
Cuts at: 0 428 669
Size: 428 241
EarI CTCTTCn'nnn_
Cuts at: 0 503 669
Size: 503 166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 182 669
Size: 131 51 487
Fragments arranged by size:
487 131 51
EcoO109I rG'GnC_Cy
Cuts at: 0 60 323 324 633 669
Size: 60 263 1 309 36
Fragments arranged by size:
309 263 60 36 1
EcoRI G'AATT_C
Cuts at: 0 94 669
Size: 94 575
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 190 669
Size: 190 479
HaeII r_GCGC'y
Cuts at: 0 303 669
Size: 303 366
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 39 71 250 282 429 461 669
Size: 39 32 179 32 147 32 208
Fragments arranged by size:
208 179 147 39 32 32 32
HincII GTy'rAC
Cuts at: 0 183 669
Size: 183 486
KasI G'GCGC_C
Cuts at: 0 299 669
Size: 299 370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 669
Size: 598 71
MslI CAynn'nnrTG
Cuts at: 0 345 669
Size: 345 324
MspA1I CmG'CkG
Cuts at: 0 489 669
Size: 489 180
NarI GG'CG_CC
Cuts at: 0 300 669
Size: 300 369
NdeI CA'TA_TG
Cuts at: 0 374 669
Size: 374 295
NspI r_CATG'y
Cuts at: 0 240 669
Size: 240 429
PspOMI G'GGCC_C
Cuts at: 0 323 669
Size: 323 346
PstI C_TGCA'G
Cuts at: 0 290 669
Size: 290 379
PvuII CAG'CTG
Cuts at: 0 489 669
Size: 489 180
SalI G'TCGA_C
Cuts at: 0 181 669
Size: 181 488
SapI GCTCTTCn'nnn_
Cuts at: 0 503 669
Size: 503 166
SbfI CC_TGCA'GG
Cuts at: 0 290 669
Size: 290 379
SfcI C'TryA_G
Cuts at: 0 286 669
Size: 286 383
SfoI GGC'GCC
Cuts at: 0 301 669
Size: 301 368
SmlI C'TyrA_G
Cuts at: 0 397 669
Size: 397 272
StuI AGG'CCT
Cuts at: 0 604 669
Size: 604 65
StyI C'CwwG_G
Cuts at: 0 455 669
Size: 455 214
TatI w'GTAC_w
Cuts at: 0 218 669
Size: 218 451
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 120 152 669
Size: 120 32 517
Fragments arranged by size:
517 120 32
Tth111I GACn'n_nGTC
Cuts at: 0 33 669
Size: 33 636
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 106 669
Size: 106 563
XmnI GAAnn'nnTTC
Cuts at: 0 625 669
Size: 625 44
Enzymes that do cut:
AarI AccI AhdI AlwNI ApaI ApoI AvaI BanI
BanII BbeI BbvCI BglI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaHI BseYI BsgI Bsp1286I BspMI BstEII BstXI
Bsu36I EarI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I
HincII KasI MmeI MslI MspA1I NarI NdeI NspI
PspOMI PstI PvuII SalI SapI SbfI SfcI SfoI
SmlI StuI StyI TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI BsaWI
BsaXI BseRI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI
EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI MscI NaeI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SanDI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII
XbaI XhoI XmaI ZraI