(Circular) (Six-base) MAPSORT of: pFN21AB4919.seq Check: 8922 from: 1 to: 10204 pFN21AB4919 10204 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2758 3504 3568 2758 Size: 746 64 9394 Fragments arranged by size: 9394 746 64 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8014 278 Size: 53 83 186 936 6478 2468 Fragments arranged by size: 6478 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 7285 7547 1937 Size: 5348 262 4594 Fragments arranged by size: 5348 4594 262 Acc65I G'GTAC_C Cuts at: 7525 7525 Size: 10204 AclI AA'CG_TT Cuts at: 1563 8331 8704 1563 Size: 6768 373 3063 Fragments arranged by size: 6768 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2054 2484 2649 3406 3539 4845 5057 6024 Size: 422 430 165 757 133 1306 212 967 Cuts at: 6024 6237 6700 6781 8267 9651 9919 1632 Size: 213 463 81 1486 1384 268 1917 Fragments arranged by size: 1917 1486 1384 1306 967 757 463 430 422 268 213 212 165 133 81 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10007 AflIII A'CryG_T Cuts at: 1203 7261 7501 9103 10080 1203 Size: 6058 240 1602 977 1327 Fragments arranged by size: 6058 1602 1327 977 240 AgeI A'CCGG_T Cuts at: 9035 9035 Size: 10204 AhdI GACnn_n'nnGTC Cuts at: 2521 8933 2521 Size: 6412 3792 AleI CACnn'nnGTG Cuts at: 1166 2324 1166 Size: 1158 9046 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3516 4502 4808 1184 Size: 2332 986 306 6580 Fragments arranged by size: 6580 2332 986 306 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4149 4150 4181 4182 4149 Size: 1 31 1 10171 Fragments arranged by size: 10171 31 1 1 AlwNI CAG_nnn'CTG Cuts at: 4348 5774 7947 9519 4348 Size: 1426 2173 1572 5033 Fragments arranged by size: 5033 2173 1572 1426 ApaLI G'TGCA_C Cuts at: 7257 8261 9417 7257 Size: 1004 1156 8044 Fragments arranged by size: 8044 1156 1004 ApoI r'AATT_y Cuts at: 1493 2915 5274 5788 7513 7739 7891 10000 1493 Size: 1422 2359 514 1725 226 152 2109 1697 Fragments arranged by size: 2359 2109 1725 1697 1422 514 226 152 AseI AT'TA_AT Cuts at: 160 1032 8758 160 Size: 872 7726 1606 Fragments arranged by size: 7726 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10204 AvaI C'yCGr_G Cuts at: 1870 1943 3607 4806 7202 7529 1870 Size: 73 1664 1199 2396 327 4545 Fragments arranged by size: 4545 2396 1664 1199 327 73 AvrII C'CTAG_G Cuts at: 2530 6398 2530 Size: 3868 6336 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4173 4206 5605 5638 4173 Size: 33 1399 33 8739 Fragments arranged by size: 8739 1399 33 33 BamHI G'GATC_C Cuts at: 2191 4176 5462 6864 7534 7901 2191 Size: 1985 1286 1402 670 367 4494 Fragments arranged by size: 4494 1985 1402 1286 670 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2200 3522 4810 6017 6850 Size: 325 217 627 413 1322 1288 1207 833 Cuts at: 6850 7525 8980 618 Size: 675 1455 1842 Fragments arranged by size: 1842 1455 1322 1288 1207 833 675 627 413 325 217 BanII G_rGCy'C Cuts at: 1391 2719 3608 4515 4910 6838 7236 7523 1391 Size: 1328 889 907 395 1928 398 287 4072 Fragments arranged by size: 4072 1928 1328 907 889 398 395 287 BbeI G_GCGC'C Cuts at: 6021 6021 Size: 10204 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4752 6787 7107 928 Size: 971 2853 2035 320 4025 Fragments arranged by size: 4025 2853 2035 971 320 BbvCI CC'TCA_GC Cuts at: 5865 5979 7312 5865 Size: 114 1333 8757 Fragments arranged by size: 8757 1333 114 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8395 8429 1903 Size: 34 6458 34 3678 Fragments arranged by size: 6458 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3804 8096 9306 3804 Size: 4292 1210 4702 Fragments arranged by size: 4702 4292 1210 BclI T'GATC_A Cuts at: 1548 5845 6069 1548 Size: 4297 224 5683 Fragments arranged by size: 5683 4297 224 BfrBI ATG'CAT Cuts at: 5296 5296 Size: 10204 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 8815 136 Size: 107 122 71 631 605 7143 1525 Fragments arranged by size: 7143 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6406 10199 6406 Size: 3793 6411 BlpI GC'TnA_GC Cuts at: 3602 3825 3870 7621 3602 Size: 223 45 3751 6185 Fragments arranged by size: 6185 3751 223 45 Bme1580I G_kGCm'C Cuts at: 1790 4813 6855 7261 8265 9421 1790 Size: 3023 2042 406 1004 1156 2573 Fragments arranged by size: 3023 2573 2042 1156 1004 406 BmgBI CAC'GTC Cuts at: 1318 5546 1318 Size: 4228 5976 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2693 5076 6117 Size: 416 53 83 399 24 1276 2383 1041 Cuts at: 6117 7935 8893 442 Size: 1818 958 1753 Fragments arranged by size: 2383 1818 1753 1276 1041 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10204 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3031 6280 6994 3031 Size: 3249 714 6241 Fragments arranged by size: 6241 3249 714 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3079 3509 4479 6291 6745 8864 1239 Size: 1840 430 970 1812 454 2119 2579 Fragments arranged by size: 2579 2119 1840 1812 970 454 430 Bpu10I CC'TnA_GC Cuts at: 3150 5865 5979 7290 7312 3150 Size: 2715 114 1311 22 6042 Fragments arranged by size: 6042 2715 1311 114 22 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2176 4719 6274 8329 9194 9492 2176 Size: 2543 1555 2055 865 298 2888 Fragments arranged by size: 2888 2543 2055 1555 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2933 6540 6723 8867 882 Size: 617 1434 3607 183 2144 2219 Fragments arranged by size: 3607 2219 2144 1434 617 183 BsaAI yAC'GTr Cuts at: 493 1204 4189 7995 493 Size: 711 2985 3806 2702 Fragments arranged by size: 3806 2985 2702 711 BsaBI GATnn'nnATC Cuts at: 10198 10198 Size: 10204 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6018 8011 8393 9028 Size: 53 83 186 936 4485 1993 382 635 Cuts at: 9028 275 Size: 1451 Fragments arranged by size: 4485 1993 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3510 4799 7173 8637 9035 9309 9456 9867 3510 Size: 1289 2374 1464 398 274 147 411 3847 Fragments arranged by size: 3847 2374 1464 1289 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 9979 Fragments arranged by size: 9979 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2504 2864 3004 3540 4592 4702 4717 5986 Size: 1318 360 140 536 1052 110 15 1269 Cuts at: 5986 6251 6256 6962 7054 7059 1186 Size: 265 5 706 92 5 4331 Fragments arranged by size: 4331 1318 1269 1052 706 536 360 265 140 110 92 15 5 5 BseYI C'CCAG_C Cuts at: 2949 3065 3283 3627 4133 4348 4644 4781 5009 Size: 116 218 344 506 215 296 137 228 Cuts at: 5009 9407 9903 2949 Size: 4398 496 3250 Fragments arranged by size: 4398 3250 506 496 344 296 228 218 215 137 116 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2754 2792 3126 3602 7247 1163 Size: 573 1018 38 334 476 3645 4120 Fragments arranged by size: 4120 3645 1018 573 476 334 38 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7654 8415 9443 1540 Size: 275 186 5653 761 1028 2301 Fragments arranged by size: 5653 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3012 3495 3608 3954 4515 7094 7261 8265 8350 Size: 483 113 346 561 2579 167 1004 85 Cuts at: 8350 9421 3012 Size: 1071 3795 Fragments arranged by size: 3795 2579 1071 1004 561 483 346 167 113 85 BsmI GAATG_Cn' Cuts at: 3172 3778 4491 7715 7808 3172 Size: 606 713 3224 93 5568 Fragments arranged by size: 5568 3224 713 606 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4877 4877 Size: 10204 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2719 3012 3495 3608 3954 4515 4813 Size: 399 929 293 483 113 346 561 298 Cuts at: 4813 4910 6838 6855 7094 7236 7261 7523 8265 Size: 97 1928 17 239 142 25 262 742 Cuts at: 8265 8350 9421 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1928 1071 929 742 561 483 399 346 298 293 262 239 142 113 97 85 25 17 BspEI T'CCGG_A Cuts at: 7173 9867 7173 Size: 2694 7510 BspHI T'CATG_A Cuts at: 8093 9823 8093 Size: 1730 8474 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2758 3504 3568 3976 4090 7559 844 Size: 1914 746 64 408 114 3469 3489 Fragments arranged by size: 3489 3469 1914 746 408 114 64 BsrBI CCG'CTC Cuts at: 1394 1823 7123 8091 1394 Size: 429 5300 968 3507 Fragments arranged by size: 5300 3507 968 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2204 4335 6920 7161 8699 8873 65 Size: 1183 956 2131 2585 241 1538 174 1396 Fragments arranged by size: 2585 2131 1538 1396 1183 956 241 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5309 8848 9035 1700 Size: 110 3499 3539 187 2869 Fragments arranged by size: 3539 3499 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4212 7151 96 Size: 4116 2939 3149 Fragments arranged by size: 4116 3149 2939 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10204 BssSI C'ACGA_G Cuts at: 8264 9276 8264 Size: 1012 9192 BstAPI GCAn_nnn'nTGC Cuts at: 794 6829 794 Size: 6035 4169 BstBI TT'CG_AA Cuts at: 9008 9008 Size: 10204 BstEII G'GTnAC_C Cuts at: 1184 4300 1184 Size: 3116 7088 BstXI CCAn_nnnn'nTGG Cuts at: 4507 4507 Size: 10204 BstYI r'GATC_y Cuts at: 1972 2191 4176 5462 6406 6864 7474 7534 7901 Size: 219 1985 1286 944 458 610 60 367 Cuts at: 7901 8286 8303 9755 9841 9853 10199 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1985 1977 1452 1286 944 610 458 385 367 346 219 86 60 17 12 Bsu36I CC'TnA_GG Cuts at: 3016 6702 6774 3016 Size: 3686 72 6446 Fragments arranged by size: 6446 3686 72 BtgI C'CryG_G Cuts at: 513 1065 2196 2394 2958 3366 3526 6028 6636 Size: 552 1131 198 564 408 160 2502 608 Cuts at: 6636 6846 7182 513 Size: 210 336 3535 Fragments arranged by size: 3535 2502 1131 608 564 552 408 336 210 198 160 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9465 Fragments arranged by size: 9465 683 56 BtsI GCAGTG_nn' Cuts at: 3614 5265 7720 8514 8534 3614 Size: 1651 2455 794 20 5284 Fragments arranged by size: 5284 2455 1651 794 20 ClaI AT'CG_AT Cuts at: 1554 4878 1554 Size: 3324 6880 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10169 DraI TTT'AAA Cuts at: 2166 7508 2166 Size: 5342 4862 DraIII CAC_nnn'GTG Cuts at: 6125 6125 Size: 10204 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2081 9211 809 Size: 507 765 7130 1802 Fragments arranged by size: 7130 1802 765 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4461 5548 7651 Size: 54 1425 50 275 18 2631 1087 2103 Cuts at: 7651 8540 10169 8 Size: 889 1629 43 Fragments arranged by size: 2631 2103 1629 1425 1087 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7651 1537 Size: 275 5839 4090 Fragments arranged by size: 5839 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2261 2277 3558 3878 4520 4944 5040 5247 Size: 908 16 1281 320 642 424 96 207 Cuts at: 5247 6451 6538 6556 6685 7182 8134 1353 Size: 1204 87 18 129 497 952 3423 Fragments arranged by size: 3423 1281 1204 952 908 642 497 424 320 207 129 96 87 18 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8791 9165 9311 387 Size: 1064 138 7202 374 146 1280 Fragments arranged by size: 7202 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3606 4513 3606 Size: 907 9297 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2484 2649 3079 3406 3509 3539 Size: 393 422 430 165 430 327 103 30 Cuts at: 3539 4479 4845 5057 6024 6237 6291 6700 6745 Size: 940 366 212 967 213 54 409 45 Cuts at: 6745 6781 8267 8864 9651 9919 1239 Size: 36 1486 597 787 268 1524 Fragments arranged by size: 1524 1486 967 940 787 597 430 430 422 409 393 366 327 268 213 212 165 103 54 45 36 30 EcoNI CCTnn'n_nnAGG Cuts at: 6271 7293 6271 Size: 1022 9182 EcoO109I rG'GnC_Cy Cuts at: 3565 3587 4621 6674 6779 6985 7022 3565 Size: 22 1034 2053 105 206 37 6747 Fragments arranged by size: 6747 2053 1034 206 105 37 22 EcoRI G'AATT_C Cuts at: 7513 7891 7513 Size: 378 9826 EcoRV GAT'ATC Cuts at: 4876 6532 4876 Size: 1656 8548 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3415 3415 Size: 10204 FspI TGC'GCA Cuts at: 8710 8710 Size: 10204 HaeII r_GCGC'y Cuts at: 1678 6021 6596 9351 1678 Size: 4343 575 2755 2531 Fragments arranged by size: 4343 2755 2531 575 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2507 2539 3259 3291 4106 Size: 32 135 32 935 32 720 32 815 Cuts at: 4106 4138 4874 4906 5447 5479 6825 6857 7466 Size: 32 736 32 541 32 1346 32 609 Cuts at: 7466 7498 8845 8877 8919 8951 1373 Size: 32 1347 32 42 32 2626 Fragments arranged by size: 2626 1347 1346 935 815 736 720 609 541 135 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4080 7548 7794 669 Size: 988 281 2142 3468 246 3079 Fragments arranged by size: 3468 3079 2142 988 281 246 HindIII A'AGCT_T Cuts at: 748 3134 3414 5315 748 Size: 2386 280 1901 5637 Fragments arranged by size: 5637 2386 1901 280 HpaI GTT'AAC Cuts at: 7794 7794 Size: 10204 KasI G'GCGC_C Cuts at: 6017 6017 Size: 10204 KpnI G_GTAC'C Cuts at: 7529 7529 Size: 10204 MfeI C'AATT_G Cuts at: 6663 7803 6663 Size: 1140 9064 MluI A'CGCG_T Cuts at: 10080 10080 Size: 10204 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2069 3706 7404 9318 9502 1305 Size: 764 1637 3698 1914 184 2007 Fragments arranged by size: 3698 2007 1914 1637 764 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4463 10 Size: 54 1425 343 2631 5751 Fragments arranged by size: 5751 2631 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2294 2324 3498 3644 4901 5048 5631 Size: 648 1128 30 1174 146 1257 147 583 Cuts at: 5631 7338 8162 8521 8680 518 Size: 1707 824 359 159 2042 Fragments arranged by size: 2042 1707 1257 1174 1128 824 648 583 359 159 147 146 30 MspA1I CmG'CkG Cuts at: 1606 2960 3287 3487 3601 4648 4945 5013 7620 Size: 1354 327 200 114 1047 297 68 2607 Cuts at: 7620 8297 9445 9690 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2607 2120 1354 1148 1047 677 327 297 245 200 114 68 NarI GG'CG_CC Cuts at: 6018 6018 Size: 10204 NcoI C'CATG_G Cuts at: 513 1065 2394 3526 6636 6846 513 Size: 552 1329 1132 3110 210 3871 Fragments arranged by size: 3871 3110 1329 1132 552 210 NdeI CA'TA_TG Cuts at: 387 4262 5700 387 Size: 3875 1438 4891 Fragments arranged by size: 4891 3875 1438 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10204 NotI GC'GGCC_GC Cuts at: 7651 7651 Size: 10204 NsiI A_TGCA'T Cuts at: 5298 5298 Size: 10204 NspI r_CATG'y Cuts at: 1132 1229 2227 3503 3944 4361 4987 5605 5830 Size: 97 998 1276 441 417 626 618 225 Cuts at: 5830 6247 7265 7505 7562 9107 1132 Size: 417 1018 240 57 1545 2229 Fragments arranged by size: 2229 1545 1276 1018 998 626 618 441 417 417 240 225 97 57 PasI CC'CwG_GG Cuts at: 1348 3445 3569 4468 6052 6273 7249 1348 Size: 2097 124 899 1584 221 976 4303 Fragments arranged by size: 4303 2097 1584 976 899 221 124 PciI A'CATG_T Cuts at: 7261 7501 9103 7261 Size: 240 1602 8362 Fragments arranged by size: 8362 1602 240 PflMI CCAn_nnn'nTGG Cuts at: 1108 4199 4348 4682 1108 Size: 3091 149 334 6630 Fragments arranged by size: 6630 3091 334 149 PfoI T'CCnGG_A Cuts at: 2548 6307 2548 Size: 3759 6445 PmeI GTTT'AAAC Cuts at: 7508 7508 Size: 10204 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8273 8305 9786 9818 8273 Size: 32 1481 32 8659 Fragments arranged by size: 8659 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3565 3587 4621 6779 6985 7022 3565 Size: 22 1034 2158 206 37 6747 Fragments arranged by size: 6747 2158 1034 206 37 22 PshAI GACnn'nnGTC Cuts at: 1613 2445 4298 1613 Size: 832 1853 7519 Fragments arranged by size: 7519 1853 832 PsiI TTA'TAA Cuts at: 7774 7774 Size: 10204 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2008 2040 2008 Size: 32 10172 PstI C_TGCA'G Cuts at: 830 2306 2364 2463 5272 7556 830 Size: 1476 58 99 2809 2284 3478 Fragments arranged by size: 3478 2809 2284 1476 99 58 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10204 PvuII CAG'CTG Cuts at: 3487 4648 4945 3487 Size: 1161 297 8746 Fragments arranged by size: 8746 1161 297 RsrII CG'GwC_CG Cuts at: 3513 3513 Size: 10204 SacI G_AGCT'C Cuts at: 3608 4515 3608 Size: 907 9297 SacII CC_GC'GG Cuts at: 2961 2961 Size: 10204 SalI G'TCGA_C Cuts at: 1936 7546 1936 Size: 5610 4594 SapI GCTCTTCn'nnn_ Cuts at: 2261 3878 4520 5247 2261 Size: 1617 642 727 7218 Fragments arranged by size: 7218 1617 727 642 SbfI CC_TGCA'GG Cuts at: 7556 7556 Size: 10204 ScaI AGT'ACT Cuts at: 8452 10164 8452 Size: 1712 8492 SfcI C'TryA_G Cuts at: 826 1045 2137 2302 2360 2459 3798 5268 7552 Size: 219 1092 165 58 99 1339 1470 2284 Cuts at: 7552 8687 9368 9559 826 Size: 1135 681 191 1471 Fragments arranged by size: 2284 1471 1470 1339 1135 1092 681 219 191 165 99 58 SfoI GGC'GCC Cuts at: 6019 6019 Size: 10204 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10204 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10204 SmaI CCC'GGG Cuts at: 1872 4808 7531 1872 Size: 2936 2723 4545 Fragments arranged by size: 4545 2936 2723 SmlI C'TyrA_G Cuts at: 820 1017 1943 2155 4734 6289 8308 9209 9471 Size: 197 926 212 2579 1555 2019 901 262 Cuts at: 9471 820 Size: 1553 Fragments arranged by size: 2579 2019 1555 1553 926 901 262 212 197 SnaBI TAC'GTA Cuts at: 493 7995 493 Size: 7502 2702 SpeI A'CTAG_T Cuts at: 152 5401 152 Size: 5249 4955 SphI G_CATG'C Cuts at: 1132 3503 4361 4987 5605 6247 7562 1132 Size: 2371 858 626 618 642 1315 3774 Fragments arranged by size: 3774 2371 1315 858 642 626 618 SspI AAT'ATT Cuts at: 5 52 2183 5496 8128 5 Size: 47 2131 3313 2632 2081 Fragments arranged by size: 3313 2632 2131 2081 47 StuI AGG'CCT Cuts at: 1517 3051 5302 1517 Size: 1534 2251 6419 Fragments arranged by size: 6419 2251 1534 StyI C'CwwG_G Cuts at: 513 1065 2394 2530 3526 6398 6503 6636 6846 Size: 552 1329 136 996 2872 105 133 210 Cuts at: 6846 6981 7643 513 Size: 135 662 3074 Fragments arranged by size: 3074 2872 1329 996 662 552 210 136 135 133 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2861 2989 3297 4047 6136 6430 6931 Size: 1255 1281 128 308 750 2089 294 501 Cuts at: 6931 7334 8231 8248 8401 8586 9031 325 Size: 403 897 17 153 185 445 1498 Fragments arranged by size: 2089 1498 1281 1255 897 750 501 445 403 308 294 185 153 128 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4212 5559 7151 Size: 274 80 33 51 1443 2235 1347 1592 Cuts at: 7151 8450 10162 96 Size: 1299 1712 138 Fragments arranged by size: 2235 1712 1592 1443 1347 1299 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4178 4210 4557 4589 6224 6256 10164 10196 4178 Size: 32 347 32 1635 32 3908 32 4186 Fragments arranged by size: 4186 3908 1635 347 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 6791 1316 Size: 5475 4729 XbaI T'CTAG_A Cuts at: 7540 7540 Size: 10204 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2462 3452 5017 5233 2462 Size: 990 1565 216 7433 Fragments arranged by size: 7433 1565 990 216 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10204 XmaI C'CCGG_G Cuts at: 1870 4806 7529 1870 Size: 2936 2723 4545 Fragments arranged by size: 4545 2936 2723 XmnI GAAnn'nnTTC Cuts at: 2916 4489 8333 2916 Size: 1573 3844 4787 Fragments arranged by size: 4787 3844 1573 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8012 276 Size: 53 83 186 936 6478 2468 Fragments arranged by size: 6478 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BsiWI BstZ17I FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI SanDI SexAI SfiI SrfI SwaI