Restriction map for
pFN21AB4514
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# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII AflII AgeI Aor51HI AscI
AseI AsnI Asp700I AvrII BfrI
BlnI BsaBI BspDI BsrBI BstBI
ClaI CpoI DrdI Eco47III EcoRI
EcoRV EcoT22I HindIII HpaI MamI
MfeI MluI MunI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshBI PvuI RsrII
SalI SbfI ScaI SexAI SfuI
SgrAI SpeI SphI Sse8387I SwaI
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 4704 GGTACC G^GTACC
AclI 4630 AACGTT AA^CGTT
Alw44I 1051 GTGCAC G^TGCAC
ApaLI 1051 GTGCAC G^TGCAC
Asp718I 4704 GGTACC G^GTACC
AspI 363 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 395 TGCGCA TGC^GCA
BcgI 3681 GCACGTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4815 GGATAC GTATCC(6/5)
BlpI 3237 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3237 GCTGAGC GC^T[ACGT]AGC
BsiEI 4406 CGGCCG CG[GA][CT]^CG
BsiWI 4517 CGTACG C^GTACG
BspLU11I 2059 ACATGT A^CATGT
BssHII 81 GCGCGC G^CGCGC
Bst1107I 4072 GTATAC GTA^TAC
BstZ17I 4072 GTATAC GTA^TAC
CelII 3237 GCTGAGC GC^T[ACGT]AGC
DraI 2907 TTTAAA TTT^AAA
DraIII 2263 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 4406 CGGCCG C^GGCCG
EclXI 4406 CGGCCG C^GGCCG
Eco52I 4406 CGGCCG C^GGCCG
FseI 4403 GGCCGGCC GGCCGG^CC
FspI 395 TGCGCA TGC^GCA
HincII 166 GTTGAC GT[CT]^[GA]AC
HindII 166 GTTGAC GT[CT]^[GA]AC
KpnI 4704 GGTACC GGTAC^C
NheI 270 GCTAGC G^CTAGC
PflFI 363 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2977 GACGAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4630 AACGTT AA^CGTT
SapI 186 GCTCTTC GCTCTTC(1/4)
SfiI 2541 GGCCTACGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 2476 TACGTA TAC^GTA
Tth111I 363 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 2620 TCCGGA T^CCGGA
3406 TCCGGA
AcsI 2004 AAATTC [GA]^AATT[CT]
3517 AAATTC
AhdI 3607 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3931 GACACATGGTC
ApoI 2004 AAATTC [GA]^AATT[CT]
3517 AAATTC
AspEI 3607 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3931 GACACATGGTC
BalI 3662 TGGCCA TGG^CCA
3794 TGGCCA
BbeI 312 GGCGCC GGCGC^C
869 GGCGCC
BbrPI 3682 CACGTG CAC^GTG
4423 CACGTG
BbsI 3447 GAAGAC GAAGAC(2/6)
4111 GAAGAC
BbvCI 974 CCTCAGC CCTCAGC(-5/-2)
3043 GCTGAGG
BclI 2513 TGATCA T^GATCA
3506 TGATCA
BglII 653 AGATCT A^GATCT
2171 AGATCT
BpuAI 3447 GAAGAC GAAGAC(2/6)
4111 GAAGAC
BseAI 2620 TCCGGA T^CCGGA
3406 TCCGGA
BsmBI 1044 CGTCTC CGTCTC(1/5)
3054 CGTCTC
BsmI 3410 GAATGC GAATGC(1/-1)
4435 GCATTC
Bsp1407I 2312 TGTACA T^GTACA
2894 TGTACA
BspEI 2620 TCCGGA T^CCGGA
3406 TCCGGA
BspHI 3164 TCATGA T^CATGA
3263 TCATGA
BsrGI 2312 TGTACA T^GTACA
2894 TGTACA
BssSI 4582 CACGAG CACGAG(-5/-1)
3130 CTCGTG
BstEII 3117 GGTGACC G^GT[ACGT]ACC
4591 GGTAACC
BstPI 3117 GGTGACC G^GT[ACGT]ACC
4591 GGTAACC
Eam1105I 3607 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3931 GACACATGGTC
EcoO65I 3117 GGTGACC G^GT[ACGT]ACC
4591 GGTAACC
FbaI 2513 TGATCA T^GATCA
3506 TGATCA
HaeII 312 GGCGCC [GA]GCGC^[CT]
869 GGCGCC
KasI 312 GGCGCC G^GCGCC
869 GGCGCC
MluNI 3662 TGGCCA TGG^CCA
3794 TGGCCA
MroI 2620 TCCGGA T^CCGGA
3406 TCCGGA
MscI 3662 TGGCCA TGG^CCA
3794 TGGCCA
NaeI 309 GCCGGC GCC^GGC
4404 GCCGGC
NarI 312 GGCGCC GG^CGCC
869 GGCGCC
NcoI 2079 CCATGG C^CATGG
3369 CCATGG
NgoMIV 309 GCCGGC G^CCGGC
4404 GCCGGC
PflMI 3778 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4499 CCAGCTTGTGG
PmaCI 3682 CACGTG CAC^GTG
4423 CACGTG
PmlI 3682 CACGTG CAC^GTG
4423 CACGTG
RcaI 3164 TCATGA T^CATGA
3263 TCATGA
SfoI 312 GGCGCC GGC^GCC
869 GGCGCC
SspBI 2312 TGTACA T^GTACA
2894 TGTACA
SspI 2938 AATATT AAT^ATT
3912 AATATT
Van91I 3778 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4499 CCAGCTTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI AspHI AvaI AvaII BamHI
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI