Restriction map for
pFN21AB4196
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# NO CUT ENZYMES ( 86 enzymes) #
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AatII AccI AccIII AflIII AgeI
Aor51HI AscI AseI AsnI AspI
AviII AvrII BbsI BclI BglI
BglII BlnI BpuAI BsaBI BsaI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EagI EclXI
Eco47III Eco52I EcoRV EcoT22I FbaI
FseI FspI HpaI KspI MamI
MluI MroI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
PshBI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 1865 GGTACC G^GTACC
AflII 994 CTTAAG C^TTAAG
AhdI 2221 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 910 GTGCAC G^TGCAC
AocI 112 CCTCAGG CC^T[ACGT]AGG
ApaI 465 GGGCCC GGGCC^C
ApaLI 910 GTGCAC G^TGCAC
Asp718I 1865 GGTACC G^GTACC
AspEI 2221 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 578 GGATCC G^GATCC
BbeI 2411 GGCGCC GGCGC^C
BbrPI 1318 CACGTG CAC^GTG
BcgI 94 CGAAGCACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2519 GTATCC GTATCC(6/5)
BfrI 994 CTTAAG C^TTAAG
BsaAI 1318 CACGTG [CT]AC^GT[GA]
BsrFI 2115 GCCGGC [GA]^CCGG[CT]
BstEII 1791 GGTCACC G^GT[ACGT]ACC
BstPI 1791 GGTCACC G^GT[ACGT]ACC
BstXI 1271 CCACAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 112 CCTCAGG CC^T[ACGT]AGG
Cfr10I 2115 GCCGGC [GA]^CCGG[CT]
DraIII 1406 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1763 CCATGG C^C[GA][CT]GG
Eam1105I 2221 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 112 CCTCAGG CC^T[ACGT]AGG
EcoNI 2059 CCTCAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1791 GGTCACC G^GT[ACGT]ACC
HindIII 316 AAGCTT A^AGCTT
KasI 2411 GGCGCC G^GCGCC
KpnI 1865 GGTACC GGTAC^C
MfeI 1428 CAATTG C^AATTG
MunI 1428 CAATTG C^AATTG
NaeI 2115 GCCGGC GCC^GGC
NarI 2411 GGCGCC GG^CGCC
NcoI 1763 CCATGG C^CATGG
NgoMIV 2115 GCCGGC G^CCGGC
PmaCI 1318 CACGTG CAC^GTG
PmlI 1318 CACGTG CAC^GTG
SexAI 1256 ACCTGGT A^CC[AT]GGT
SfoI 2411 GGCGCC GGC^GCC
SmaI 2708 CCCGGG CCC^GGG
TaqI 2567 TCGA T^CGA
TthHB8I 2567 TCGA T^CGA
XmaCI 2708 CCCGGG C^CCGGG
XmaI 2708 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccII 280 CGCG CG^CG
2552 CGCG
AclI 1367 AACGTT AA^CGTT
1888 AACGTT
AvaI 461 CTCGGG C^[CT]CG[GA]G
2708 CCCGGG
BsaWI 1868 ACCGGA [AT]^CCGG[AT]
2387 ACCGGA
BsgI 140 CTGCAC GTGCAG(16/14)
1945 CTGCAC
BsmBI 2110 GAGACG CGTCTC(1/5)
2548 GAGACG
BsoBI 461 CTCGGG C^[CT]CG[GA]G
2708 CCCGGG
BspHI 48 TCATGA T^CATGA
1605 TCATGA
BsrBI 2031 CCGCTC CCGCTC(-3/-3)
679 GAGCGG
BstUI 280 CGCG CG^CG
2552 CGCG
EcoRI 841 GAATTC G^AATTC
927 GAATTC
EcoT14I 1590 CCTTGG C^C[AT][AT]GG
1763 CCATGG
FauI 650 CCCGC CCCGC(4/6)
1473 GCGGG
HaeII 1583 AGCGCC [GA]GCGC^[CT]
2411 GGCGCC
MvnI 280 CGCG CG^CG
2552 CGCG
Psp1406I 1367 AACGTT AA^CGTT
1888 AACGTT
RcaI 48 TCATGA T^CATGA
1605 TCATGA
SmlI 149 CTCAAG C^T[CT][GA]AG
994 CTTAAG
SphI 1501 GCATGC GCATG^C
2555 GCATGC
StuI 293 AGGCCT AGG^CCT
500 AGGCCT
StyI 1590 CCTTGG C^C[AT][AT]GG
1763 CCATGG
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# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI XhoII XmnI