Restriction map for
pFN21AB4196
(Linear) (Six-base) MAPSORT of: KIBB4196.seq Check: 7062 from: 1 to: 2871
KIBB4196.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 109 2871
Size: 109 2762
Acc65I G'GTAC_C
Cuts at: 0 1865 2871
Size: 1865 1006
AclI AA'CG_TT
Cuts at: 0 1368 1889 2871
Size: 1368 521 982
Fragments arranged by size:
1368 982 521
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 1519 1530 1897 2437 2871
Size: 304 1215 11 367 540 434
Fragments arranged by size:
1215 540 434 367 304 11
AflII C'TTAA_G
Cuts at: 0 994 2871
Size: 994 1877
AhdI GACnn_n'nnGTC
Cuts at: 0 2226 2871
Size: 2226 645
AleI CACnn'nnGTG
Cuts at: 0 1519 1592 2871
Size: 1519 73 1279
Fragments arranged by size:
1519 1279 73
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1960 2871
Size: 1960 911
AlwNI CAG_nnn'CTG
Cuts at: 0 939 1734 2262 2607 2871
Size: 939 795 528 345 264
Fragments arranged by size:
939 795 528 345 264
ApaI G_GGCC'C
Cuts at: 0 469 2871
Size: 469 2402
ApaLI G'TGCA_C
Cuts at: 0 910 2871
Size: 910 1961
ApoI r'AATT_y
Cuts at: 0 841 927 2026 2871
Size: 841 86 1099 845
Fragments arranged by size:
1099 845 841 86
AvaI C'yCGr_G
Cuts at: 0 461 2708 2871
Size: 461 2247 163
Fragments arranged by size:
2247 461 163
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1522 1555 1857 1890 2871
Size: 1522 33 302 33 981
Fragments arranged by size:
1522 981 302 33 33
BamHI G'GATC_C
Cuts at: 0 578 2871
Size: 578 2293
BanI G'GyrC_C
Cuts at: 0 205 1620 1865 2411 2445 2871
Size: 205 1415 245 546 34 426
Fragments arranged by size:
1415 546 426 245 205 34
BanII G_rGCy'C
Cuts at: 0 469 776 1673 1757 2871
Size: 469 307 897 84 1114
Fragments arranged by size:
1114 897 469 307 84
BbeI G_GCGC'C
Cuts at: 0 2415 2871
Size: 2415 456
BbvCI CC'TCA_GC
Cuts at: 0 749 2060 2407 2441 2871
Size: 749 1311 347 34 430
Fragments arranged by size:
1311 749 430 347 34
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 83 117 2871
Size: 83 34 2754
Fragments arranged by size:
2754 83 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2530 2871
Size: 2530 341
BlpI GC'TnA_GC
Cuts at: 0 352 380 1802 2871
Size: 352 28 1422 1069
Fragments arranged by size:
1422 1069 352 28
Bme1580I G_kGCm'C
Cuts at: 0 469 914 1625 1849 2871
Size: 469 445 711 224 1022
Fragments arranged by size:
1022 711 469 445 224
BmrI ACTGGGnnnn_n'
Cuts at: 0 1667 1722 1752 2444 2819 2871
Size: 1667 55 30 692 375 52
Fragments arranged by size:
1667 692 375 55 52 30
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 315 2871
Size: 315 2556
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 709 988 1756 1771 2113 2259 2380
Size: 508 201 279 768 15 342 146 121
Cuts at: 2380 2566 2714 2871
Size: 186 148 157
Fragments arranged by size:
768 508 342 279 201 186 157 148
146 121 15
Bpu10I CC'TnA_GC
Cuts at: 0 175 497 749 2060 2407 2441 2871
Size: 175 322 252 1311 347 34 430
Fragments arranged by size:
1311 430 347 322 252 175 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 2871
Size: 134 2737
BsaAI yAC'GTr
Cuts at: 0 1320 2871
Size: 1320 1551
BsaHI Gr'CG_yC
Cuts at: 0 976 2113 2412 2871
Size: 976 1137 299 459
Fragments arranged by size:
1137 976 459 299
BsaWI w'CCGG_w
Cuts at: 0 1868 2387 2871
Size: 1868 519 484
Fragments arranged by size:
1868 519 484
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1033 1063 1744 1774 2137 2167 2871
Size: 1033 30 681 30 363 30 704
Fragments arranged by size:
1033 704 681 363 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 137 382 541 550 1041 1044 1390 1426
Size: 137 245 159 9 491 3 346 36
Cuts at: 1426 2446 2512 2871
Size: 1020 66 359
Fragments arranged by size:
1020 491 359 346 245 159 137 66
36 9 3
BseYI C'CCAG_C
Cuts at: 0 160 512 1136 1632 2594 2871
Size: 160 352 624 496 962 277
Fragments arranged by size:
962 624 496 352 277 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 1930 2871
Size: 125 1805 941
Fragments arranged by size:
1805 941 125
BsiHKAI G_wGCw'C
Cuts at: 0 812 914 1319 1831 2871
Size: 812 102 405 512 1040
Fragments arranged by size:
1040 812 512 405 102
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2104 2542 2871
Size: 2104 438 329
Fragments arranged by size:
2104 438 329
Bsp1286I G_dGCh'C
Cuts at: 0 469 776 812 914 1319 1625 1673 1757
Size: 469 307 36 102 405 306 48 84
Cuts at: 1757 1831 1849 2871
Size: 74 18 1022
Fragments arranged by size:
1022 469 405 307 306 102 84 74
48 36 18
BspHI T'CATG_A
Cuts at: 0 48 1605 2871
Size: 48 1557 1266
Fragments arranged by size:
1557 1266 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 109 1915 2270 2871
Size: 109 1806 355 601
Fragments arranged by size:
1806 601 355 109
BsrBI CCG'CTC
Cuts at: 0 681 2033 2871
Size: 681 1352 838
Fragments arranged by size:
1352 838 681
BsrDI GCAATG_nn'
Cuts at: 0 722 1496 1668 1796 2499 2871
Size: 722 774 172 128 703 372
Fragments arranged by size:
774 722 703 372 172 128
BsrFI r'CCGG_y
Cuts at: 0 2115 2871
Size: 2115 756
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1302 1502 2088 2871
Size: 1302 200 586 783
Fragments arranged by size:
1302 783 586 200
BstEII G'GTnAC_C
Cuts at: 0 1791 2871
Size: 1791 1080
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1278 2871
Size: 1278 1593
BstYI r'GATC_y
Cuts at: 0 578 783 2360 2561 2871
Size: 578 205 1577 201 310
Fragments arranged by size:
1577 578 310 205 201
Bsu36I CC'TnA_GG
Cuts at: 0 113 2871
Size: 113 2758
BtgI C'CryG_G
Cuts at: 0 1763 2871
Size: 1763 1108
BtsI GCAGTG_nn'
Cuts at: 0 1211 1344 1849 2331 2655 2871
Size: 1211 133 505 482 324 216
Fragments arranged by size:
1211 505 482 324 216 133
DraIII CAC_nnn'GTG
Cuts at: 0 1411 2871
Size: 1411 1460
EaeI y'GGCC_r
Cuts at: 0 620 1556 2134 2330 2507 2871
Size: 620 936 578 196 177 364
Fragments arranged by size:
936 620 578 364 196 177
EarI CTCTTCn'nnn_
Cuts at: 0 335 793 1549 2871
Size: 335 458 756 1322
Fragments arranged by size:
1322 756 458 335
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 508 709 988 1519 1530 1756 1771
Size: 304 204 201 279 531 11 226 15
Cuts at: 1771 1897 2113 2259 2380 2437 2566 2714 2871
Size: 126 216 146 121 57 129 148 157
Fragments arranged by size:
531 304 279 226 216 204 201 157
148 146 129 126 121 57 15 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2063 2871
Size: 2063 808
EcoO109I rG'GnC_Cy
Cuts at: 0 465 466 936 2871
Size: 465 1 470 1935
Fragments arranged by size:
1935 470 465 1
EcoRI G'AATT_C
Cuts at: 0 841 927 2871
Size: 841 86 1944
Fragments arranged by size:
1944 841 86
HaeII r_GCGC'y
Cuts at: 0 1587 2415 2871
Size: 1587 828 456
Fragments arranged by size:
1587 828 456
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 113 145 358 390 570 602 1744 1776
Size: 113 32 213 32 180 32 1142 32
Cuts at: 1776 2137 2169 2181 2212 2213 2244 2743 2775
Size: 361 32 12 31 1 31 499 32
Cuts at: 2775 2871
Size: 96
Fragments arranged by size:
1142 499 361 213 180 113 96 32
32 32 32 32 32 31 31 12
1
HincII GTy'rAC
Cuts at: 0 644 1398 1746 1893 2871
Size: 644 754 348 147 978
Fragments arranged by size:
978 754 644 348 147
HindIII A'AGCT_T
Cuts at: 0 316 2871
Size: 316 2555
KasI G'GCGC_C
Cuts at: 0 2411 2871
Size: 2411 460
KpnI G_GTAC'C
Cuts at: 0 1869 2871
Size: 1869 1002
MfeI C'AATT_G
Cuts at: 0 1428 2871
Size: 1428 1443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 2871
Size: 226 2645
MscI TGG'CCA
Cuts at: 0 622 1558 2136 2332 2871
Size: 622 936 578 196 539
Fragments arranged by size:
936 622 578 539 196
MslI CAynn'nnrTG
Cuts at: 0 1216 1506 1519 1592 1618 2492 2871
Size: 1216 290 13 73 26 874 379
Fragments arranged by size:
1216 874 379 290 73 26 13
MspA1I CmG'CkG
Cuts at: 0 64 384 576 653 1463 1632 2440 2598
Size: 64 320 192 77 810 169 808 158
Cuts at: 2598 2871
Size: 273
Fragments arranged by size:
810 808 320 273 192 169 158 77
64
NaeI GCC'GGC
Cuts at: 0 2117 2871
Size: 2117 754
NarI GG'CG_CC
Cuts at: 0 2412 2871
Size: 2412 459
NcoI C'CATG_G
Cuts at: 0 1763 2871
Size: 1763 1108
NgoMIV G'CCGG_C
Cuts at: 0 2115 2871
Size: 2115 756
NspI r_CATG'y
Cuts at: 0 436 1136 1505 2559 2871
Size: 436 700 369 1054 312
Fragments arranged by size:
1054 700 436 369 312
PasI CC'CwG_GG
Cuts at: 0 1070 2871
Size: 1070 1801
PflMI CCAn_nnn'nTGG
Cuts at: 0 939 1136 1734 1764 1769 2305 2871
Size: 939 197 598 30 5 536 566
Fragments arranged by size:
939 598 566 536 197 30 5
PfoI T'CCnGG_A
Cuts at: 0 1004 1733 2871
Size: 1004 729 1138
Fragments arranged by size:
1138 1004 729
PmlI CAC'GTG
Cuts at: 0 1320 2871
Size: 1320 1551
PspOMI G'GGCC_C
Cuts at: 0 465 2871
Size: 465 2406
PstI C_TGCA'G
Cuts at: 0 1307 2603 2627 2661 2748 2871
Size: 1307 1296 24 34 87 123
Fragments arranged by size:
1307 1296 123 87 34 24
PvuII CAG'CTG
Cuts at: 0 64 384 1632 2440 2598 2871
Size: 64 320 1248 808 158 273
Fragments arranged by size:
1248 808 320 273 158 64
SexAI A'CCwGG_T
Cuts at: 0 1256 2871
Size: 1256 1615
SfcI C'TryA_G
Cuts at: 0 391 970 1303 1836 2103 2353 2599 2623
Size: 391 579 333 533 267 250 246 24
Cuts at: 2623 2657 2744 2871
Size: 34 87 127
Fragments arranged by size:
579 533 391 333 267 250 246 127
87 34 24
SfoI GGC'GCC
Cuts at: 0 2413 2871
Size: 2413 458
SmaI CCC'GGG
Cuts at: 0 2710 2871
Size: 2710 161
SmlI C'TyrA_G
Cuts at: 0 149 994 2871
Size: 149 845 1877
Fragments arranged by size:
1877 845 149
SphI G_CATG'C
Cuts at: 0 1505 2559 2871
Size: 1505 1054 312
Fragments arranged by size:
1505 1054 312
StuI AGG'CCT
Cuts at: 0 295 502 2871
Size: 295 207 2369
Fragments arranged by size:
2369 295 207
StyI C'CwwG_G
Cuts at: 0 1590 1763 2871
Size: 1590 173 1108
Fragments arranged by size:
1590 1108 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 533 1104 1624 2463 2646 2871
Size: 533 571 520 839 183 225
Fragments arranged by size:
839 571 533 520 225 183
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1033 1065 2871
Size: 1033 32 1806
Fragments arranged by size:
1806 1033 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1021 1588 1770 2859 2871
Size: 1021 567 182 1089 12
Fragments arranged by size:
1089 1021 567 182 12
XmaI C'CCGG_G
Cuts at: 0 2708 2871
Size: 2708 163
XmnI GAAnn'nnTTC
Cuts at: 0 1532 1882 2026 2871
Size: 1532 350 144 845
Fragments arranged by size:
1532 845 350 144
Enzymes that do cut:
AarI Acc65I AclI AcuI AflII AhdI AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtsI DraIII EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PflMI PfoI PmlI PspOMI PstI PvuII
SexAI SfcI SfoI SmaI SmlI SphI StuI StyI
TaqII TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AfeI AflIII AgeI AloI AscI AseI
AsiSI AvrII BbsI BclI BfrBI BglI BglII BmgBI
BmtI BsaI BsaBI BsiEI BsiWI BsmI BspEI BsrGI
BssHII BssSI BstBI BstZ17I BtgZI ClaI CspCI DraI
DrdI EagI EciI EcoICRI EcoRV FalI FseI FspI
FspAI HpaI MluI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI TatI Tth111I XbaI XhoI ZraI