Restriction map for
pFN21AB4032
(Linear) (Six-base) MAPSORT of: KIBB4032.seq Check: 7115 from: 1 to: 3402
KIBB4032.orf
With 182 enzymes: SgfI *
November 13, 2006 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1758 3402
Size: 1758 1644
AccI GT'mk_AC
Cuts at: 0 1078 1406 1855 3402
Size: 1078 328 449 1547
Fragments arranged by size:
1547 1078 449 328
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 394 1142 1565 1794 2048 2675 3402
Size: 176 218 748 423 229 254 627 727
Fragments arranged by size:
748 727 627 423 254 229 218 176
AfeI AGC'GCT
Cuts at: 0 481 3402
Size: 481 2921
AflIII A'CryG_T
Cuts at: 0 2394 2586 3402
Size: 2394 192 816
Fragments arranged by size:
2394 816 192
AhdI GACnn_n'nnGTC
Cuts at: 0 786 3402
Size: 786 2616
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 542 3119 3129 3402
Size: 542 2577 10 273
Fragments arranged by size:
2577 542 273 10
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1993 2025 3402
Size: 1993 32 1377
Fragments arranged by size:
1993 1377 32
AlwNI CAG_nnn'CTG
Cuts at: 0 799 1185 2044 2056 2344 3402
Size: 799 386 859 12 288 1058
Fragments arranged by size:
1058 859 799 386 288 12
ApaI G_GGCC'C
Cuts at: 0 2663 3402
Size: 2663 739
ApoI r'AATT_y
Cuts at: 0 2093 2785 2830 3402
Size: 2093 692 45 572
Fragments arranged by size:
2093 692 572 45
AscI GG'CGCG_CC
Cuts at: 0 3316 3402
Size: 3316 86
AseI AT'TA_AT
Cuts at: 0 1544 3402
Size: 1544 1858
AvrII C'CTAG_G
Cuts at: 0 382 3402
Size: 382 3020
BamHI G'GATC_C
Cuts at: 0 309 3402
Size: 309 3093
BanI G'GyrC_C
Cuts at: 0 1869 3402
Size: 1869 1533
BanII G_rGCy'C
Cuts at: 0 701 2405 2663 3402
Size: 701 1704 258 739
Fragments arranged by size:
1704 739 701 258
BbeI G_GCGC'C
Cuts at: 0 1873 3402
Size: 1873 1529
BbsI GAAGACnn'nnnn_
Cuts at: 0 2675 3293 3402
Size: 2675 618 109
Fragments arranged by size:
2675 618 109
BbvCI CC'TCA_GC
Cuts at: 0 940 1357 3402
Size: 940 417 2045
Fragments arranged by size:
2045 940 417
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1659 1693 3402
Size: 1659 34 1709
Fragments arranged by size:
1709 1659 34
BglI GCCn_nnn'nGGC
Cuts at: 0 275 1101 2658 3402
Size: 275 826 1557 744
Fragments arranged by size:
1557 826 744 275
BglII A'GATC_T
Cuts at: 0 301 1198 1272 3402
Size: 301 897 74 2130
Fragments arranged by size:
2130 897 301 74
BlpI GC'TnA_GC
Cuts at: 0 530 581 800 3402
Size: 530 51 219 2602
Fragments arranged by size:
2602 530 219 51
Bme1580I G_kGCm'C
Cuts at: 0 2663 3402
Size: 2663 739
BmgBI CAC'GTC
Cuts at: 0 108 3402
Size: 108 3294
BmrI ACTGGGnnnn_n'
Cuts at: 0 1325 2362 3402
Size: 1325 1037 1040
Fragments arranged by size:
1325 1040 1037
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2328 2436 3402
Size: 2328 108 966
Fragments arranged by size:
2328 966 108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 1709 2483 2533 2626 3402
Size: 71 1638 774 50 93 776
Fragments arranged by size:
1638 776 774 93 71 50
Bpu10I CC'TnA_GC
Cuts at: 0 940 1357 1481 3402
Size: 940 417 124 1921
Fragments arranged by size:
1921 940 417 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 1083 3402
Size: 736 347 2319
Fragments arranged by size:
2319 736 347
BsaI GGTCTCn'nnnn_
Cuts at: 0 2299 2424 2715 3402
Size: 2299 125 291 687
Fragments arranged by size:
2299 687 291 125
BsaAI yAC'GTr
Cuts at: 0 615 3349 3402
Size: 615 2734 53
Fragments arranged by size:
2734 615 53
BsaBI GATnn'nnATC
Cuts at: 0 1010 3402
Size: 1010 2392
BsaHI Gr'CG_yC
Cuts at: 0 1870 3402
Size: 1870 1532
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1707 1737 3053 3083 3402
Size: 1707 30 1316 30 319
Fragments arranged by size:
1707 1316 319 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2623 3402
Size: 2623 779
BseYI C'CCAG_C
Cuts at: 0 449 1019 1687 1778 3357 3402
Size: 449 570 668 91 1579 45
Fragments arranged by size:
1579 668 570 449 91 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1918 2942 3402
Size: 1918 1024 460
Fragments arranged by size:
1918 1024 460
BsiHKAI G_wGCw'C
Cuts at: 0 603 701 3148 3402
Size: 603 98 2447 254
Fragments arranged by size:
2447 603 254 98
BsmI GAATG_Cn'
Cuts at: 0 864 1326 2466 3402
Size: 864 462 1140 936
Fragments arranged by size:
1140 936 864 462
BsmBI CGTCTCn'nnnn_
Cuts at: 0 98 3402
Size: 98 3304
Bsp1286I G_dGCh'C
Cuts at: 0 603 701 2405 2663 3148 3402
Size: 603 98 1704 258 485 254
Fragments arranged by size:
1704 603 485 258 254 98
BspHI T'CATG_A
Cuts at: 0 261 3402
Size: 261 3141
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1758 2697 3402
Size: 1758 939 705
Fragments arranged by size:
1758 939 705
BsrDI GCAATG_nn'
Cuts at: 0 642 3402
Size: 642 2760
BsrFI r'CCGG_y
Cuts at: 0 947 3402
Size: 947 2455
BsrGI T'GTAC_A
Cuts at: 0 2841 3402
Size: 2841 561
BssHII G'CGCG_C
Cuts at: 0 3316 3402
Size: 3316 86
BstAPI GCAn_nnn'nTGC
Cuts at: 0 459 1896 3402
Size: 459 1437 1506
Fragments arranged by size:
1506 1437 459
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1243 3402
Size: 1243 2159
BstYI r'GATC_y
Cuts at: 0 114 301 309 390 1198 1272 3402
Size: 114 187 8 81 808 74 2130
Fragments arranged by size:
2130 808 187 114 81 74 8
BstZ17I GTA'TAC
Cuts at: 0 1856 3402
Size: 1856 1546
Bsu36I CC'TnA_GG
Cuts at: 0 2519 3402
Size: 2519 883
BtgI C'CryG_G
Cuts at: 0 1634 1826 2300 3402
Size: 1634 192 474 1102
Fragments arranged by size:
1634 1102 474 192
BtsI GCAGTG_nn'
Cuts at: 0 800 1183 1464 3402
Size: 800 383 281 1938
Fragments arranged by size:
1938 800 383 281
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1028 1063 3335 3370 3402
Size: 1028 35 2272 35 32
Fragments arranged by size:
2272 1028 35 35 32
DraI TTT'AAA
Cuts at: 0 320 3402
Size: 320 3082
DraIII CAC_nnn'GTG
Cuts at: 0 3143 3402
Size: 3143 259
EaeI y'GGCC_r
Cuts at: 0 1704 2101 2297 3402
Size: 1704 397 196 1105
Fragments arranged by size:
1704 1105 397 196
EarI CTCTTCn'nnn_
Cuts at: 0 619 2909 3402
Size: 619 2290 493
Fragments arranged by size:
2290 619 493
EcoICRI GAG'CTC
Cuts at: 0 699 3402
Size: 699 2703
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 176 394 1142 1565 1709 1794 2048
Size: 71 105 218 748 423 144 85 254
Cuts at: 2048 2483 2533 2626 2675 3402
Size: 435 50 93 49 727
Fragments arranged by size:
748 727 435 423 254 218 144 105
93 85 71 50 49
EcoNI CCTnn'n_nnAGG
Cuts at: 0 817 2498 3402
Size: 817 1681 904
Fragments arranged by size:
1681 904 817
EcoO109I rG'GnC_Cy
Cuts at: 0 1060 2245 2659 3402
Size: 1060 1185 414 743
Fragments arranged by size:
1185 1060 743 414
EcoRI G'AATT_C
Cuts at: 0 2093 3402
Size: 2093 1309
EcoRV GAT'ATC
Cuts at: 0 1467 3402
Size: 1467 1935
HaeII r_GCGC'y
Cuts at: 0 483 1873 3402
Size: 483 1390 1529
Fragments arranged by size:
1529 1390 483
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 772 804 1707 1739 1902 1934 3402
Size: 772 32 903 32 163 32 1468
Fragments arranged by size:
1468 903 772 163 32 32 32
HincII GTy'rAC
Cuts at: 0 1619 1910 3402
Size: 1619 291 1492
Fragments arranged by size:
1619 1492 291
HindIII A'AGCT_T
Cuts at: 0 2269 3402
Size: 2269 1133
KasI G'GCGC_C
Cuts at: 0 1869 3402
Size: 1869 1533
MfeI C'AATT_G
Cuts at: 0 457 3402
Size: 457 2945
MscI TGG'CCA
Cuts at: 0 1706 3402
Size: 1706 1696
MspA1I CmG'CkG
Cuts at: 0 1356 3067 3402
Size: 1356 1711 335
Fragments arranged by size:
1711 1356 335
NarI GG'CG_CC
Cuts at: 0 1870 3402
Size: 1870 1532
NcoI C'CATG_G
Cuts at: 0 1634 2300 3402
Size: 1634 666 1102
Fragments arranged by size:
1634 1102 666
NspI r_CATG'y
Cuts at: 0 2398 2487 2590 2685 3096 3402
Size: 2398 89 103 95 411 306
Fragments arranged by size:
2398 411 306 103 95 89
PciI A'CATG_T
Cuts at: 0 2394 2586 3402
Size: 2394 192 816
Fragments arranged by size:
2394 816 192
PflMI CCAn_nnn'nTGG
Cuts at: 0 1827 3402
Size: 1827 1575
PmlI CAC'GTG
Cuts at: 0 615 3402
Size: 615 2787
PpuMI rG'GwC_Cy
Cuts at: 0 1060 2245 3402
Size: 1060 1185 1157
Fragments arranged by size:
1185 1157 1060
PspOMI G'GGCC_C
Cuts at: 0 2659 3402
Size: 2659 743
PstI C_TGCA'G
Cuts at: 0 795 830 1056 3108 3402
Size: 795 35 226 2052 294
Fragments arranged by size:
2052 795 294 226 35
SacI G_AGCT'C
Cuts at: 0 701 3402
Size: 701 2701
SapI GCTCTTCn'nnn_
Cuts at: 0 2909 3402
Size: 2909 493
SfcI C'TryA_G
Cuts at: 0 791 826 973 1052 3084 3104 3402
Size: 791 35 147 79 2032 20 298
Fragments arranged by size:
2032 791 298 147 79 35 20
SfoI GGC'GCC
Cuts at: 0 1871 3402
Size: 1871 1531
SmlI C'TyrA_G
Cuts at: 0 751 1098 3402
Size: 751 347 2304
Fragments arranged by size:
2304 751 347
SphI G_CATG'C
Cuts at: 0 2685 3402
Size: 2685 717
SspI AAT'ATT
Cuts at: 0 2262 3402
Size: 2262 1140
StuI AGG'CCT
Cuts at: 0 1104 2208 2504 3402
Size: 1104 1104 296 898
Fragments arranged by size:
1104 1104 898 296
StyI C'CwwG_G
Cuts at: 0 382 1142 1287 1634 2203 2300 3402
Size: 382 760 145 347 569 97 1102
Fragments arranged by size:
1102 760 569 382 347 145 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2009 3402
Size: 2009 1393
TatI w'GTAC_w
Cuts at: 0 2467 2841 3402
Size: 2467 374 561
Fragments arranged by size:
2467 561 374
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 60 92 937 969 1785 1817 2765 2797
Size: 60 32 845 32 816 32 948 32
Cuts at: 2797 3053 3085 3402
Size: 256 32 317
Fragments arranged by size:
948 845 816 317 256 60 32 32
32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2026 2298 3157 3402
Size: 2026 272 859 245
Fragments arranged by size:
2026 859 272 245
XmnI GAAnn'nnTTC
Cuts at: 0 2093 3402
Size: 2093 1309
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AlfI AloI
AlwNI ApaI ApoI AscI AseI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI
BssHII BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtsI
CspCI DraI DraIII EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV HaeII Hin4I HincII HindIII KasI
MfeI MscI MspA1I NarI NcoI NspI PciI PflMI
PmlI PpuMI PspOMI PstI SacI SapI SfcI SfoI
SmlI SphI SspI StuI StyI TaqII TatI TstI
XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AleI ApaLI AsiSI
AvaI BaeI BciVI BclI BfrBI BmtI BsaWI BsiEI
BsiWI BspEI BsrBI BssSI BstBI BstEII BtgZI ClaI
DrdI EagI EciI FalI FseI FspI FspAI HpaI
KpnI MluI MmeI MslI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PfoI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SwaI Tth111I XbaI XhoI
XmaI ZraI