Restriction map for
pFN21AB3954
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# NO CUT ENZYMES ( 91 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI AscI Asp700I
Asp718I AspEI AviII BbeI BbvCI
BcgI BfrI BglII BlpI Bpu1102I
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII Bst1107I
BstBI BstXI BstZ17I CelII ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV FseI FspI
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PflMI PinAI
PmeI PshAI Psp1406I PvuI RcaI
RsrII SacII SapI ScaI SfoI
SfuI SgrAI SnaBI SpeI SspBI
SspI StuI SwaI Van91I XbaI
XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccII 1345 CGCG CG^CG
AcyI 1582 GGCGTC G[GA]^CG[CT]C
AflIII 3972 ACACGT A^C[GA][CT]GT
Alw44I 1997 GTGCAC G^TGCAC
ApaLI 1997 GTGCAC G^TGCAC
AseI 1162 ATTAAT AT^TAAT
AsnI 1162 ATTAAT AT^TAAT
AspI 1685 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2899 CCTAGG C^CTAGG
BclI 4356 TGATCA T^GATCA
BlnI 2899 CCTAGG C^CTAGG
BsaHI 1582 GGCGTC G[GA]^CG[CT]C
BstAPI 2172 GCAAGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1345 CGCG CG^CG
CfoI 3131 GCGC GCG^C
EarI 3032 GAAGAG CTCTTC(1/4)
EcoRI 4195 GAATTC G^AATTC
EcoT22I 3903 ATGCAT ATGCA^T
FbaI 4356 TGATCA T^GATCA
HaeII 3130 GGCGCT [GA]GCGC^[CT]
HgaI 1583 GCGTC GACGC(5/10)
HhaI 3131 GCGC GCG^C
Hin1I 1582 GGCGTC G[GA]^CG[CT]C
HinP1I 3131 GCGC G^CGC
Ksp632I 3032 GAAGAG CTCTTC(1/4)
MslI 1126 CACGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1345 CGCG CG^CG
NdeI 470 CATATG CA^TATG
NsiI 3903 ATGCAT ATGCA^T
PflFI 1685 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1162 ATTAAT AT^TAAT
SalI 1682 GTCGAC G^TCGAC
SmaI 1235 CCCGGG CCC^GGG
Tth111I 1685 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1235 CCCGGG C^CCGGG
XmaI 1235 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccI 701 GTAGAC GT^[AC][GT]AC
1682 GTCGAC
BalI 2319 TGGCCA TGG^CCA
2778 TGGCCA
BamHI 172 GGATCC G^GATCC
1033 GGATCC
BbrPI 1334 CACGTG CAC^GTG
3269 CACGTG
BciVI 592 GGATAC GTATCC(6/5)
764 GGATAC
BsaAI 1334 CACGTG [CT]AC^GT[GA]
3269 CACGTG
BsrDI 3612 CATTGC GCAATG(2/0)
4125 CATTGC
BsrFI 1255 GCCGGT [GA]^CCGG[CT]
1993 GCCGGT
BssSI 2378 CTCGTG CACGAG(-5/-1)
2630 CTCGTG
BstEII 361 GGTGACC G^GT[ACGT]ACC
2662 GGTAACC
BstPI 361 GGTGACC G^GT[ACGT]ACC
2662 GGTAACC
Cfr10I 1255 GCCGGT [GA]^CCGG[CT]
1993 GCCGGT
EcoNI 1930 CCTGGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3841 CCTAACCAAGG
EcoO65I 361 GGTGACC G^GT[ACGT]ACC
2662 GGTAACC
MluNI 2319 TGGCCA TGG^CCA
2778 TGGCCA
MscI 2319 TGGCCA TGG^CCA
2778 TGGCCA
NcoI 893 CCATGG C^CATGG
3992 CCATGG
PaeR7I 974 CTCGAG C^TCGAG
3494 CTCGAG
PmaCI 1334 CACGTG CAC^GTG
3269 CACGTG
PmlI 1334 CACGTG CAC^GTG
3269 CACGTG
SphI 1610 GCATGC GCATG^C
2888 GCATGC
XhoI 974 CTCGAG C^TCGAG
3494 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraII DraIII DrdI DsaI EaeI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
SbfI ScrFI SexAI SfaNI SfcI
SfiI SmlI Sse8387I StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII