Restriction map for
pFN21AB3954
(Linear) (Six-base) MAPSORT of: KIBB3954.seq Check: 5541 from: 1 to: 4398
KIBB3954.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 940 1282 4398
Size: 940 342 3116
Fragments arranged by size:
3116 940 342
AccI GT'mk_AC
Cuts at: 0 702 1683 4398
Size: 702 981 2715
Fragments arranged by size:
2715 981 702
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 4058 4189 4226 4398
Size: 617 3441 131 37 172
Fragments arranged by size:
3441 617 172 131 37
AflIII A'CryG_T
Cuts at: 0 3972 4398
Size: 3972 426
AleI CACnn'nnGTG
Cuts at: 0 1130 4398
Size: 1130 3268
AlwNI CAG_nnn'CTG
Cuts at: 0 1441 1540 2251 2422 2719 3367 3403 4398
Size: 1441 99 711 171 297 648 36 995
Fragments arranged by size:
1441 995 711 648 297 171 99 36
ApaI G_GGCC'C
Cuts at: 0 1550 2073 2711 2818 4398
Size: 1550 523 638 107 1580
Fragments arranged by size:
1580 1550 638 523 107
ApaLI G'TGCA_C
Cuts at: 0 1997 4398
Size: 1997 2401
ApoI r'AATT_y
Cuts at: 0 233 3796 4195 4219 4363 4398
Size: 233 3563 399 24 144 35
Fragments arranged by size:
3563 399 233 144 35 24
AseI AT'TA_AT
Cuts at: 0 1163 4398
Size: 1163 3235
AvaI C'yCGr_G
Cuts at: 0 956 974 1235 1712 3242 3494 4398
Size: 956 18 261 477 1530 252 904
Fragments arranged by size:
1530 956 904 477 261 252 18
AvrII C'CTAG_G
Cuts at: 0 2899 4398
Size: 2899 1499
BamHI G'GATC_C
Cuts at: 0 172 1033 4398
Size: 172 861 3365
Fragments arranged by size:
3365 861 172
BanI G'GyrC_C
Cuts at: 0 1087 1312 1456 2032 2347 2806 3121 3346
Size: 1087 225 144 576 315 459 315 225
Cuts at: 3346 3571 4398
Size: 225 827
Fragments arranged by size:
1087 827 576 459 315 315 225 225
225 144
BanII G_rGCy'C
Cuts at: 0 885 912 1218 1236 1328 1550 2010 2073
Size: 885 27 306 18 92 222 460 63
Cuts at: 2073 2487 2711 2818 2829 3529 4398
Size: 414 224 107 11 700 869
Fragments arranged by size:
885 869 700 460 414 306 224 222
107 92 63 27 18 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 856 2629 2989 3052 4398
Size: 856 1773 360 63 1346
Fragments arranged by size:
1773 1346 856 360 63
BciVI GTATCCnnnnn_n'
Cuts at: 0 586 758 4398
Size: 586 172 3640
Fragments arranged by size:
3640 586 172
BclI T'GATC_A
Cuts at: 0 4356 4398
Size: 4356 42
BfrBI ATG'CAT
Cuts at: 0 3905 4398
Size: 3905 493
BglI GCCn_nnn'nGGC
Cuts at: 0 536 1869 2318 3209 4398
Size: 536 1333 449 891 1189
Fragments arranged by size:
1333 1189 891 536 449
Bme1580I G_kGCm'C
Cuts at: 0 1092 1461 1550 2001 2037 2073 2352 2711
Size: 1092 369 89 451 36 36 279 359
Cuts at: 2711 2811 2818 3126 3351 3576 4398
Size: 100 7 308 225 225 822
Fragments arranged by size:
1092 822 451 369 359 308 279 225
225 100 89 36 36 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 453 1877 3425 3840 3902 4271 4398
Size: 453 1424 1548 415 62 369 127
Fragments arranged by size:
1548 1424 453 415 369 127 62
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 572 626 725 737 1229 1232 1430
Size: 506 66 54 99 12 492 3 198
Cuts at: 1430 1517 1538 1583 1610 1817 1931 1994 2024
Size: 87 21 45 27 207 114 63 30
Cuts at: 2024 2204 2348 2399 2537 2555 2678 2771 2813
Size: 180 144 51 138 18 123 93 42
Cuts at: 2813 2879 3344 4065 4258 4398
Size: 66 465 721 193 140
Fragments arranged by size:
721 506 492 465 207 198 193 180
144 140 138 123 114 99 93 87
66 66 63 54 51 45 42 30
27 21 18 12 3
Bpu10I CC'TnA_GC
Cuts at: 0 1046 4398
Size: 1046 3352
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 1121 3245 3697 3799 4398
Size: 296 825 2124 452 102 599
Fragments arranged by size:
2124 825 599 452 296 102
BsaI GGTCTCn'nnnn_
Cuts at: 0 1076 2965 3782 4398
Size: 1076 1889 817 616
Fragments arranged by size:
1889 1076 817 616
BsaAI yAC'GTr
Cuts at: 0 1336 3271 4398
Size: 1336 1935 1127
Fragments arranged by size:
1935 1336 1127
BsaHI Gr'CG_yC
Cuts at: 0 1583 4398
Size: 1583 2815
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 276 306 345 375 479 509 822 852
Size: 276 30 39 30 104 30 313 30
Cuts at: 852 2397 2427 2721 2751 4398
Size: 1545 30 294 30 1647
Fragments arranged by size:
1647 1545 313 294 276 104 39 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1118 1568 2207 3206 4398
Size: 1118 450 639 999 1192
Fragments arranged by size:
1192 1118 999 639 450
BseYI C'CCAG_C
Cuts at: 0 760 2313 2683 2848 3616 4398
Size: 760 1553 370 165 768 782
Fragments arranged by size:
1553 782 768 760 370 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 1310 4075 4398
Size: 275 1035 2765 323
Fragments arranged by size:
2765 1035 323 275
BsiHKAI G_wGCw'C
Cuts at: 0 840 885 957 975 1137 1218 1272 1533
Size: 840 45 72 18 162 81 54 261
Cuts at: 1533 1767 2001 2010 2442 2631 2739 2829 2865
Size: 234 234 9 432 189 108 90 36
Cuts at: 2865 3225 3252 4398
Size: 360 27 1146
Fragments arranged by size:
1146 840 432 360 261 234 234 189
162 108 90 81 72 54 45 36
27 18 9
BsmI GAATG_Cn'
Cuts at: 0 1205 2183 4063 4398
Size: 1205 978 1880 335
Fragments arranged by size:
1880 1205 978 335
Bsp1286I G_dGCh'C
Cuts at: 0 840 885 912 957 975 1092 1137 1218
Size: 840 45 27 45 18 117 45 81
Cuts at: 1218 1236 1272 1328 1461 1533 1550 1767 2001
Size: 18 36 56 133 72 17 217 234
Cuts at: 2001 2010 2037 2073 2352 2442 2487 2631 2711
Size: 9 27 36 279 90 45 144 80
Cuts at: 2711 2739 2811 2818 2829 2865 3126 3225 3252
Size: 28 72 7 11 36 261 99 27
Cuts at: 3252 3351 3529 3576 4398
Size: 99 178 47 822
Fragments arranged by size:
840 822 279 261 234 217 178 144
133 117 99 99 90 81 80 72
72 56 47 45 45 45 45 36
36 36 28 27 27 27 18 18
17 11 9 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 940 1071 1282 1485 2430 2556 3411 4398
Size: 940 131 211 203 945 126 855 987
Fragments arranged by size:
987 945 940 855 211 203 131 126
BsrDI GCAATG_nn'
Cuts at: 0 3611 4124 4398
Size: 3611 513 274
Fragments arranged by size:
3611 513 274
BsrFI r'CCGG_y
Cuts at: 0 1255 1993 4398
Size: 1255 738 2405
Fragments arranged by size:
2405 1255 738
BssSI C'ACGA_G
Cuts at: 0 2378 2630 4398
Size: 2378 252 1768
Fragments arranged by size:
2378 1768 252
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2178 4398
Size: 2178 2220
BstEII G'GTnAC_C
Cuts at: 0 361 2662 4398
Size: 361 2301 1736
Fragments arranged by size:
2301 1736 361
BstYI r'GATC_y
Cuts at: 0 172 327 583 1033 3076 3517 4398
Size: 172 155 256 450 2043 441 881
Fragments arranged by size:
2043 881 450 441 256 172 155
Bsu36I CC'TnA_GG
Cuts at: 0 1118 1811 1847 2918 4398
Size: 1118 693 36 1071 1480
Fragments arranged by size:
1480 1118 1071 693 36
BtgI C'CryG_G
Cuts at: 0 893 3931 3992 4398
Size: 893 3038 61 406
Fragments arranged by size:
3038 893 406 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 200 4398
Size: 200 4198
BtsI GCAGTG_nn'
Cuts at: 0 987 4110 4398
Size: 987 3123 288
Fragments arranged by size:
3123 987 288
DraIII CAC_nnn'GTG
Cuts at: 0 1339 2149 3274 4398
Size: 1339 810 1125 1124
Fragments arranged by size:
1339 1125 1124 810
DrdI GACnn_nn'nnGTC
Cuts at: 0 896 1904 2552 2678 2993 3470 4398
Size: 896 1008 648 126 315 477 928
Fragments arranged by size:
1008 928 896 648 477 315 126
EaeI y'GGCC_r
Cuts at: 0 928 2319 2778 4398
Size: 928 1391 459 1620
Fragments arranged by size:
1620 1391 928 459
EarI CTCTTCn'nnn_
Cuts at: 0 3027 4398
Size: 3027 1371
EcoICRI GAG'CTC
Cuts at: 0 883 1216 2008 2827 4398
Size: 883 333 792 819 1571
Fragments arranged by size:
1571 883 819 792 333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 572 617 626 725 737 1229 1232
Size: 506 66 45 9 99 12 492 3
Cuts at: 1232 1430 1517 1538 1583 1610 1817 1931 1994
Size: 198 87 21 45 27 207 114 63
Cuts at: 1994 2024 2204 2348 2399 2537 2555 2678 2771
Size: 30 180 144 51 138 18 123 93
Cuts at: 2771 2813 2879 3344 4058 4065 4189 4226 4258
Size: 42 66 465 714 7 124 37 32
Cuts at: 4258 4398
Size: 140
Fragments arranged by size:
714 506 492 465 207 198 180 144
140 138 124 123 114 99 93 87
66 66 63 51 45 45 42 37
32 30 27 21 18 12 9 7
3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1934 3845 4398
Size: 1934 1911 553
Fragments arranged by size:
1934 1911 553
EcoO109I rG'GnC_Cy
Cuts at: 0 307 316 334 389 538 601 637 646
Size: 307 9 18 55 149 63 36 9
Cuts at: 646 997 1141 1195 1240 1474 1510 1537 1546
Size: 351 144 54 45 234 36 27 9
Cuts at: 1546 1654 1690 1801 1816 1852 1861 1897 2069
Size: 108 36 111 15 36 9 36 172
Cuts at: 2069 2070 2284 2419 2509 2527 2671 2680 2708
Size: 1 214 135 90 18 144 9 28
Cuts at: 2708 2716 2814 2896 3364 3400 3409 3418 3472
Size: 8 98 82 468 36 9 9 54
Cuts at: 3472 3490 3553 4164 4398
Size: 18 63 611 234
Fragments arranged by size:
611 468 351 307 234 234 214 172
149 144 144 135 111 108 98 90
82 63 63 55 54 54 45 36
36 36 36 36 36 28 27 18
18 18 15 9 9 9 9 9
9 9 8 1
EcoRI G'AATT_C
Cuts at: 0 4195 4398
Size: 4195 203
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4063 4398
Size: 4063 335
HaeII r_GCGC'y
Cuts at: 0 3134 4398
Size: 3134 1264
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 157 164 189 196 466 498 679 711
Size: 157 7 25 7 270 32 181 32
Cuts at: 711 1568 1600 2782 2814 4398
Size: 857 32 1182 32 1584
Fragments arranged by size:
1584 1182 857 270 181 157 32 32
32 32 25 7 7
HincII GTy'rAC
Cuts at: 0 991 1684 3837 4398
Size: 991 693 2153 561
Fragments arranged by size:
2153 991 693 561
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 1038 3405 3579 4398
Size: 298 740 2367 174 819
Fragments arranged by size:
2367 819 740 298 174
MscI TGG'CCA
Cuts at: 0 2321 2780 4398
Size: 2321 459 1618
Fragments arranged by size:
2321 1618 459
MslI CAynn'nnrTG
Cuts at: 0 1130 4398
Size: 1130 3268
MspA1I CmG'CkG
Cuts at: 0 526 760 1201 2317 2687 3019 3172 3607
Size: 526 234 441 1116 370 332 153 435
Cuts at: 3607 4059 4398
Size: 452 339
Fragments arranged by size:
1116 526 452 441 435 370 339 332
234 153
NcoI C'CATG_G
Cuts at: 0 893 3992 4398
Size: 893 3099 406
Fragments arranged by size:
3099 893 406
NdeI CA'TA_TG
Cuts at: 0 471 4398
Size: 471 3927
NsiI A_TGCA'T
Cuts at: 0 3907 4398
Size: 3907 491
NspI r_CATG'y
Cuts at: 0 1614 2892 3905 4398
Size: 1614 1278 1013 493
Fragments arranged by size:
1614 1278 1013 493
PasI CC'CwG_GG
Cuts at: 0 1462 1858 2263 2811 2991 3541 4398
Size: 1462 396 405 548 180 550 857
Fragments arranged by size:
1462 857 550 548 405 396 180
PfoI T'CCnGG_A
Cuts at: 0 375 516 588 642 840 885 1011 1209
Size: 375 141 72 54 198 45 126 198
Cuts at: 1209 1218 1245 1272 1371 1434 1596 1623 1659
Size: 9 27 27 99 63 162 27 36
Cuts at: 1659 1821 1947 2010 2055 2415 2442 2514 3396
Size: 162 126 63 45 360 27 72 882
Cuts at: 3396 3558 4398
Size: 162 840
Fragments arranged by size:
882 840 375 360 198 198 162 162
162 141 126 126 99 72 72 63
63 54 45 45 36 27 27 27
27 9
PmlI CAC'GTG
Cuts at: 0 1336 3271 4398
Size: 1336 1935 1127
Fragments arranged by size:
1935 1336 1127
PpuMI rG'GwC_Cy
Cuts at: 0 307 389 601 646 1141 1195 1240 1474
Size: 307 82 212 45 495 54 45 234
Cuts at: 1474 1510 1690 1801 1816 1852 1897 2284 2419
Size: 36 180 111 15 36 45 387 135
Cuts at: 2419 2527 2671 2680 2716 2896 3400 3418 3472
Size: 108 144 9 36 180 504 18 54
Cuts at: 3472 4398
Size: 926
Fragments arranged by size:
926 504 495 387 307 234 212 180
180 144 135 111 108 82 54 54
45 45 45 36 36 36 18 15
9
PspOMI G'GGCC_C
Cuts at: 0 1546 2069 2707 2814 4398
Size: 1546 523 638 107 1584
Fragments arranged by size:
1584 1546 638 523 107
PspXI vC'TCGA_Gb
Cuts at: 0 974 3494 4398
Size: 974 2520 904
Fragments arranged by size:
2520 974 904
PstI C_TGCA'G
Cuts at: 0 951 1068 1446 1545 3408 3787 4398
Size: 951 117 378 99 1863 379 611
Fragments arranged by size:
1863 951 611 379 378 117 99
PvuII CAG'CTG
Cuts at: 0 526 760 2317 3019 3172 4059 4398
Size: 526 234 1557 702 153 887 339
Fragments arranged by size:
1557 887 702 526 339 234 153
SacI G_AGCT'C
Cuts at: 0 885 1218 2010 2829 4398
Size: 885 333 792 819 1569
Fragments arranged by size:
1569 885 819 792 333
SalI G'TCGA_C
Cuts at: 0 1682 4398
Size: 1682 2716
SanDI GG'GwC_CC
Cuts at: 0 389 1195 1852 4398
Size: 389 806 657 2546
Fragments arranged by size:
2546 806 657 389
SbfI CC_TGCA'GG
Cuts at: 0 1068 1545 3408 4398
Size: 1068 477 1863 990
Fragments arranged by size:
1863 1068 990 477
SexAI A'CCwGG_T
Cuts at: 0 1416 1686 1974 2145 2460 2604 3153 4398
Size: 1416 270 288 171 315 144 549 1245
Fragments arranged by size:
1416 1245 549 315 288 270 171 144
SfcI C'TryA_G
Cuts at: 0 659 947 1064 1442 1541 3404 3783 4398
Size: 659 288 117 378 99 1863 379 615
Fragments arranged by size:
1863 659 615 379 378 288 117 99
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 536 1869 2318 3209 4398
Size: 536 1333 449 891 1189
Fragments arranged by size:
1333 1189 891 536 449
SmaI CCC'GGG
Cuts at: 0 1237 4398
Size: 1237 3161
SmlI C'TyrA_G
Cuts at: 0 311 974 1136 3260 3494 3712 3814 4398
Size: 311 663 162 2124 234 218 102 584
Fragments arranged by size:
2124 663 584 311 234 218 162 102
SphI G_CATG'C
Cuts at: 0 1614 2892 4398
Size: 1614 1278 1506
Fragments arranged by size:
1614 1506 1278
StyI C'CwwG_G
Cuts at: 0 320 597 893 2278 2568 2899 3846 3992
Size: 320 277 296 1385 290 331 947 146
Cuts at: 3992 4398
Size: 406
Fragments arranged by size:
1385 947 406 331 320 296 290 277
146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3154 3554 4398
Size: 3154 400 844
Fragments arranged by size:
3154 844 400
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 345 377 2721 2753 3612 3644 4398
Size: 345 32 2344 32 859 32 754
Fragments arranged by size:
2344 859 754 345 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1688 4398
Size: 1688 2710
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 60 604 2998 3938 4398
Size: 60 544 2394 940 460
Fragments arranged by size:
2394 940 544 460 60
XhoI C'TCGA_G
Cuts at: 0 974 3494 4398
Size: 974 2520 904
Fragments arranged by size:
2520 974 904
XmaI C'CCGG_G
Cuts at: 0 1235 4398
Size: 1235 3163
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlwNI ApaI ApaLI
ApoI AseI AvaI AvrII BamHI BanI BanII BbsI
BciVI BclI BfrBI BglI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BssSI BstAPI
BstEII BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HincII MmeI MscI MslI MspA1I NcoI
NdeI NsiI NspI PasI PfoI PmlI PpuMI PspOMI
PspXI PstI PvuII SacI SalI SanDI SbfI SexAI
SfcI SfiI SmaI SmlI SphI StyI TaqII TstI
Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI AscI AsiSI BaeI BbeI BbvCI BcgI BglII
BlpI BmgBI BmtI BplI BsaBI BsaWI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrGI BssHII BstBI BstXI
BstZ17I ClaI CspCI DraI EagI EciI EcoRV FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NgoMIV NheI NotI NruI PacI PciI
PflMI PmeI PpiI PshAI PsiI PsrI PvuI RsrII
SacII SapI ScaI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI StuI SwaI TatI XbaI XmnI ZraI