Restriction map for
pFN21AB3497
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# NO CUT ENZYMES ( 88 enzymes) #
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AatII AccIII AcyI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BcgI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaHI BsaWI BseAI BsiWI Bsp1407I
BspEI BsrGI BssHII BstBI BstEII
BstPI CelII CpoI DraIII DrdI
Eam1105I Eco47III EcoNI EcoO65I FbaI
FseI FspI HaeII Hin1I KasI
KspI MfeI MluI MroI MunI
NarI NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PvuI RsrII
SacI SacII SalI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI SspBI SspI StuI SwaI
Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 414 GGTACC G^GTACC
AccI 1167 GTATAC GT^[AC][GT]AC
AccII 1903 CGCG CG^CG
AclI 3137 AACGTT AA^CGTT
AflIII 1487 ACATGT A^C[GA][CT]GT
AlwNI 2018 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 4175 GGGCCC GGGCC^C
AseI 3531 ATTAAT AT^TAAT
AsnI 3531 ATTAAT AT^TAAT
Asp718I 414 GGTACC G^GTACC
BcnI 2802 CCCGG CC^[GC]GG
BsaBI 2192 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 521 GAGACC GGTCTC(1/5)
BsiEI 2501 CGGCCG CG[GA][CT]^CG
BsmBI 3924 GAGACG CGTCTC(1/5)
BspDI 1555 ATCGAT AT^CGAT
BspLU11I 1487 ACATGT A^CATGT
BsrBI 3457 GAGCGG CCGCTC(-3/-3)
BsrFI 3634 GCCGGC [GA]^CCGG[CT]
BssSI 3574 CACGAG CACGAG(-5/-1)
Bst1107I 1167 GTATAC GTA^TAC
BstAPI 3898 GCAAAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1903 CGCG CG^CG
BstXI 4246 CCAGAATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1167 GTATAC GTA^TAC
Cfr10I 3634 GCCGGC [GA]^CCGG[CT]
ClaI 1555 ATCGAT AT^CGAT
DraI 528 TTTAAA TTT^AAA
EagI 2501 CGGCCG C^GGCCG
EclXI 2501 CGGCCG C^GGCCG
Eco52I 2501 CGGCCG C^GGCCG
EcoRI 4135 GAATTC G^AATTC
EcoRV 2419 GATATC GAT^ATC
EcoT22I 3955 ATGCAT ATGCA^T
HgaI 3877 GCGTC GACGC(5/10)
HindIII 3072 AAGCTT A^AGCTT
KpnI 414 GGTACC GGTAC^C
MamI 2192 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1903 CGCG CG^CG
NaeI 3634 GCCGGC GCC^GGC
NciI 2802 CCCGG CC^[GC]GG
NgoMIV 3634 GCCGGC G^CCGGC
NsiI 3955 ATGCAT ATGCA^T
PaeR7I 1145 CTCGAG C^TCGAG
PflMI 4063 CCACCATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 3531 ATTAAT AT^TAAT
Psp1406I 3137 AACGTT AA^CGTT
Van91I 4063 CCACCATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1963 TCTAGA T^CTAGA
XhoI 1145 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AocI 1822 CCTCAGG CC^T[ACGT]AGG
3474 CCTCAGG
AspHI 550 GAGCAC G[AT]GC[AT]^C
1094 GTGCTC
AvaI 914 CCCGAG C^[CT]CG[GA]G
1145 CTCGAG
BalI 132 TGGCCA TGG^CCA
2920 TGGCCA
BanII 3569 GAGCCC G[GA]GC[CT]^C
4175 GGGCCC
BbvCI 2383 CCTCAGC CCTCAGC(-5/-2)
1021 GCTGAGG
BseRI 404 CTCCTC GAGGAG(10/8)
1317 CTCCTC
BsiHKAI 550 GAGCAC G[AT]GC[AT]^C
1094 GTGCTC
BsoBI 914 CCCGAG C^[CT]CG[GA]G
1145 CTCGAG
Bsu36I 1822 CCTCAGG CC^T[ACGT]AGG
3474 CCTCAGG
CfoI 630 GCGC GCG^C
1904 GCGC
Eco81I 1822 CCTCAGG CC^T[ACGT]AGG
3474 CCTCAGG
FauI 3287 CCCGC CCCGC(4/6)
3372 CCCGC
HhaI 630 GCGC GCG^C
1904 GCGC
HinP1I 630 GCGC G^CGC
1904 GCGC
HpaI 3603 GTTAAC GTT^AAC
3768 GTTAAC
MluNI 132 TGGCCA TGG^CCA
2920 TGGCCA
MscI 132 TGGCCA TGG^CCA
2920 TGGCCA
NcoI 2054 CCATGG C^CATGG
4229 CCATGG
SbfI 1980 CCTGCAGG CCTGCA^GG
3754 CCTGCAGG
ScaI 726 AGTACT AGT^ACT
1330 AGTACT
SpeI 1927 ACTAGT A^CTAGT
3091 ACTAGT
Sse8387I 1980 CCTGCAGG CCTGCA^GG
3754 CCTGCAGG
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# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BanI BbsI
BbvI BciVI BfaI BglII BmyI
BpmI BpuAI BsaJI BsgI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspHI BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RcaI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI