Restriction map for
pFN21AB3179
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaLI AscI AspEI AvaI
AviII AvrII BbeI BbrPI BbvCI
BcgI BfrI BglI BlnI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BsrBI BsrGI BssHII BssSI BstEII
BstPI BstUI CfoI ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I FseI FspI HaeII
HhaI Hin1I HinP1I HpaI KasI
KspI MluI MroI MvnI NaeI
NarI NgoMIV NheI NotI NruI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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Acc65I 3385 GGTACC G^GTACC
AseI 3030 ATTAAT AT^TAAT
AsnI 3030 ATTAAT AT^TAAT
Asp718I 3385 GGTACC G^GTACC
BalI 1230 TGGCCA TGG^CCA
BanI 3385 GGTACC G^G[CT][GA]CC
BclI 831 TGATCA T^GATCA
BlpI 2704 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2704 GCTGAGC GC^T[ACGT]AGC
BsaBI 2729 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1790 GAGGAG GAGGAG(10/8)
BspLU11I 1613 ACATGT A^CATGT
BsrFI 2915 ACCGGC [GA]^CCGG[CT]
CelII 2704 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2915 ACCGGC [GA]^CCGG[CT]
DraIII 2031 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1230 TGGCCA [CT]^GGCC[GA]
EcoRV 2401 GATATC GAT^ATC
EcoT22I 4289 ATGCAT ATGCA^T
FauI 3382 GCGGG CCCGC(4/6)
FbaI 831 TGATCA T^GATCA
HgaI 3344 GACGC GACGC(5/10)
KpnI 3385 GGTACC GGTAC^C
MamI 2729 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1230 TGGCCA TGG^CCA
MscI 1230 TGGCCA TGG^CCA
NsiI 4289 ATGCAT ATGCA^T
PshBI 3030 ATTAAT AT^TAAT
PvuII 4539 CAGCTG CAG^CTG
SacI 4168 GAGCTC GAGCT^C
SapI 1918 GCTCTTC GCTCTTC(1/4)
SexAI 1279 ACCAGGT A^CC[AT]GGT
SwaI 4040 ATTTAAAT ATTT^AAAT
XcmI 2690 CCAGATTAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AflIII 339 ACGTGT A^C[GA][CT]GT
1613 ACATGT
AocI 1437 CCTAAGG CC^T[ACGT]AGG
3390 CCTGAGG
ApaI 2479 GGGCCC GGGCC^C
4407 GGGCCC
AspHI 755 GAGCAC G[AT]GC[AT]^C
4168 GAGCTC
AspI 1690 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2902 GACTATGTC
BamHI 680 GGATCC G^GATCC
1804 GGATCC
BcnI 1420 CCGGG CC^[GC]GG
4410 CCCGG
BglII 1683 AGATCT A^GATCT
2881 AGATCT
BsaAI 338 TACGTG [CT]AC^GT[GA]
1600 CACGTA
BsaI 3400 GGTCTC GGTCTC(1/5)
3963 GGTCTC
BsiHKAI 755 GAGCAC G[AT]GC[AT]^C
4168 GAGCTC
BspHI 1758 TCATGA T^CATGA
3924 TCATGA
BstAPI 1738 GCAATGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1948 GCATGTTGTGC
Bsu36I 1437 CCTAAGG CC^T[ACGT]AGG
3390 CCTGAGG
DraI 2620 TTTAAA TTT^AAA
4041 TTTAAA
DsaI 1233 CCATGG C^C[GA][CT]GG
1319 CCATGG
Eco81I 1437 CCTAAGG CC^T[ACGT]AGG
3390 CCTGAGG
EcoNI 1820 CCTGTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4486 CCTTGGAAAGG
HincII 916 GTCAAC GT[CT]^[GA]AC
2641 GTTGAC
HindII 916 GTCAAC GT[CT]^[GA]AC
2641 GTTGAC
MspA1I 3288 CCGCTG C[AC]G^C[GT]G
4539 CAGCTG
NciI 1420 CCGGG CC^[GC]GG
4410 CCCGG
NcoI 1233 CCATGG C^CATGG
1319 CCATGG
NdeI 838 CATATG CA^TATG
3968 CATATG
PflFI 1690 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2902 GACTATGTC
PflMI 151 CCATTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3832 CCAAGAGATGG
PleI 492 GACTC GAGTC(4/5)
1160 GACTC
RcaI 1758 TCATGA T^CATGA
3924 TCATGA
ScaI 1927 AGTACT AGT^ACT
4526 AGTACT
SspI 3364 AATATT AAT^ATT
3910 AATATT
StuI 1828 AGGCCT AGG^CCT
4132 AGGCCT
Tth111I 1690 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2902 GACTATGTC
Van91I 151 CCATTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3832 CCAAGAGATGG
XbaI 2239 TCTAGA T^CTAGA
3513 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI Asp700I AvaII BanII
BbsI BbvI BciVI BfaI BmyI
BpmI BpuAI BsaJI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrDI BsrI BssKI Bst1107I BstBI
BstF5I BstNI BstXI BstYI BstZ17I
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MnlI MseI MslI
MspI MunI MvaI MwoI NdeII
NlaIII NlaIV NspI NspV PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfuI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI