Restriction map for
pFN21AB3179
(Linear) (Six-base) MAPSORT of: KIBB3179.seq Check: 5889 from: 1 to: 4596
KIBB3179.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3189 4596
Size: 3189 1407
AccI GT'mk_AC
Cuts at: 0 139 574 650 3327 4596
Size: 139 435 76 2677 1269
Fragments arranged by size:
2677 1269 435 139 76
Acc65I G'GTAC_C
Cuts at: 0 3385 4596
Size: 3385 1211
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 370 2036 2066 2966 3004 4019 4596
Size: 51 319 1666 30 900 38 1015 577
Fragments arranged by size:
1666 1015 900 577 319 51 38 30
AflIII A'CryG_T
Cuts at: 0 339 1613 4596
Size: 339 1274 2983
Fragments arranged by size:
2983 1274 339
AleI CACnn'nnGTG
Cuts at: 0 4073 4596
Size: 4073 523
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1209 2302 4596
Size: 1209 1093 2294
Fragments arranged by size:
2294 1209 1093
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1796 1828 4596
Size: 1796 32 2768
Fragments arranged by size:
2768 1796 32
ApaI G_GGCC'C
Cuts at: 0 2483 4411 4596
Size: 2483 1928 185
Fragments arranged by size:
2483 1928 185
ApoI r'AATT_y
Cuts at: 0 23 181 717 843 898 1143 1632 2379
Size: 23 158 536 126 55 245 489 747
Cuts at: 2379 2506 2582 2666 3154 3428 4277 4596
Size: 127 76 84 488 274 849 319
Fragments arranged by size:
849 747 536 489 488 319 274 245
158 127 126 84 76 55 23
AseI AT'TA_AT
Cuts at: 0 3031 4596
Size: 3031 1565
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3432 3465 4596
Size: 3432 33 1131
Fragments arranged by size:
3432 1131 33
BamHI G'GATC_C
Cuts at: 0 680 1804 4596
Size: 680 1124 2792
Fragments arranged by size:
2792 1124 680
BanI G'GyrC_C
Cuts at: 0 3385 4596
Size: 3385 1211
BanII G_rGCy'C
Cuts at: 0 2483 4172 4411 4596
Size: 2483 1689 239 185
Fragments arranged by size:
2483 1689 239 185
BbsI GAAGACnn'nnnn_
Cuts at: 0 948 2186 2797 4034 4596
Size: 948 1238 611 1237 562
Fragments arranged by size:
1238 1237 948 611 562
BciVI GTATCCnnnnn_n'
Cuts at: 0 3051 3297 3490 3557 4596
Size: 3051 246 193 67 1039
Fragments arranged by size:
3051 1039 246 193 67
BclI T'GATC_A
Cuts at: 0 831 4596
Size: 831 3765
BfrBI ATG'CAT
Cuts at: 0 4291 4596
Size: 4291 305
BglII A'GATC_T
Cuts at: 0 1683 2881 4596
Size: 1683 1198 1715
Fragments arranged by size:
1715 1683 1198
BlpI GC'TnA_GC
Cuts at: 0 2705 4596
Size: 2705 1891
Bme1580I G_kGCm'C
Cuts at: 0 1521 2483 3532 4411 4596
Size: 1521 962 1049 879 185
Fragments arranged by size:
1521 1049 962 879 185
BmrI ACTGGGnnnn_n'
Cuts at: 0 1720 3427 3526 4518 4596
Size: 1720 1707 99 992 78
Fragments arranged by size:
1720 1707 992 99 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 2483 2507 2673 2906 4596
Size: 179 2304 24 166 233 1690
Fragments arranged by size:
2304 1690 233 179 166 24
Bpu10I CC'TnA_GC
Cuts at: 0 4129 4166 4596
Size: 4129 37 430
Fragments arranged by size:
4129 430 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3508 4495 4596
Size: 3508 987 101
Fragments arranged by size:
3508 987 101
BsaI GGTCTCn'nnnn_
Cuts at: 0 3406 3969 4596
Size: 3406 563 627
Fragments arranged by size:
3406 627 563
BsaAI yAC'GTr
Cuts at: 0 340 1602 4596
Size: 340 1262 2994
Fragments arranged by size:
2994 1262 340
BsaBI GATnn'nnATC
Cuts at: 0 2733 4596
Size: 2733 1863
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1454 1484 1784 1814 4596
Size: 1454 30 300 30 2782
Fragments arranged by size:
2782 1454 300 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1805 4596
Size: 1805 2791
BseYI C'CCAG_C
Cuts at: 0 1457 3293 3822 4596
Size: 1457 1836 529 774
Fragments arranged by size:
1836 1457 774 529
BsiHKAI G_wGCw'C
Cuts at: 0 759 4172 4596
Size: 759 3413 424
Fragments arranged by size:
3413 759 424
BsmI GAATG_Cn'
Cuts at: 0 1111 3666 4524 4596
Size: 1111 2555 858 72
Fragments arranged by size:
2555 1111 858 72
Bsp1286I G_dGCh'C
Cuts at: 0 759 1521 2483 3532 4172 4411 4596
Size: 759 762 962 1049 640 239 185
Fragments arranged by size:
1049 962 762 759 640 239 185
BspHI T'CATG_A
Cuts at: 0 1758 3924 4596
Size: 1758 2166 672
Fragments arranged by size:
2166 1758 672
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 744 822 2791 3189 4596
Size: 744 78 1969 398 1407
Fragments arranged by size:
1969 1407 744 398 78
BsrDI GCAATG_nn'
Cuts at: 0 484 1052 1745 2050 3241 3626 4157 4596
Size: 484 568 693 305 1191 385 531 439
Fragments arranged by size:
1191 693 568 531 484 439 385 305
BsrFI r'CCGG_y
Cuts at: 0 2915 4596
Size: 2915 1681
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1744 1954 4596
Size: 1744 210 2642
Fragments arranged by size:
2642 1744 210
BstBI TT'CG_AA
Cuts at: 0 21 1404 2589 4596
Size: 21 1383 1185 2007
Fragments arranged by size:
2007 1383 1185 21
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3054 4152 4404 4596
Size: 3054 1098 252 192
Fragments arranged by size:
3054 1098 252 192
BstYI r'GATC_y
Cuts at: 0 680 1011 1683 1804 2652 2717 2881 2990
Size: 680 331 672 121 848 65 164 109
Cuts at: 2990 4251 4480 4596
Size: 1261 229 116
Fragments arranged by size:
1261 848 680 672 331 229 164 121
116 109 65
BstZ17I GTA'TAC
Cuts at: 0 140 575 3328 4596
Size: 140 435 2753 1268
Fragments arranged by size:
2753 1268 435 140
Bsu36I CC'TnA_GG
Cuts at: 0 1438 3391 4596
Size: 1438 1953 1205
Fragments arranged by size:
1953 1438 1205
BtgI C'CryG_G
Cuts at: 0 1233 1319 4596
Size: 1233 86 3277
Fragments arranged by size:
3277 1233 86
BtsI GCAGTG_nn'
Cuts at: 0 3481 4596
Size: 3481 1115
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4139 4174 4596
Size: 4139 35 422
Fragments arranged by size:
4139 422 35
DraI TTT'AAA
Cuts at: 0 2622 4043 4596
Size: 2622 1421 553
Fragments arranged by size:
2622 1421 553
DraIII CAC_nnn'GTG
Cuts at: 0 2036 4596
Size: 2036 2560
EaeI y'GGCC_r
Cuts at: 0 1230 4596
Size: 1230 3366
EarI CTCTTCn'nnn_
Cuts at: 0 70 389 1298 1406 1601 1757 1925 3056
Size: 70 319 909 108 195 156 168 1131
Cuts at: 3056 4596
Size: 1540
Fragments arranged by size:
1540 1131 909 319 195 168 156 108
70
EcoICRI GAG'CTC
Cuts at: 0 4170 4596
Size: 4170 426
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 179 370 2036 2066 2483 2507 2673
Size: 51 128 191 1666 30 417 24 166
Cuts at: 2673 2906 2966 3004 4019 4596
Size: 233 60 38 1015 577
Fragments arranged by size:
1666 1015 577 417 233 191 166 128
60 51 38 30 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1824 4490 4596
Size: 1824 2666 106
Fragments arranged by size:
2666 1824 106
EcoO109I rG'GnC_Cy
Cuts at: 0 412 1249 2479 4342 4596
Size: 412 837 1230 1863 254
Fragments arranged by size:
1863 1230 837 412 254
EcoRI G'AATT_C
Cuts at: 0 717 843 2379 2582 4596
Size: 717 126 1536 203 2014
Fragments arranged by size:
2014 1536 717 203 126
EcoRV GAT'ATC
Cuts at: 0 2403 4596
Size: 2403 2193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 81 2344 2868 3323 4596
Size: 81 2263 524 455 1273
Fragments arranged by size:
2263 1273 524 455 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3384 3416 4596
Size: 3384 32 1180
Fragments arranged by size:
3384 1180 32
HincII GTy'rAC
Cuts at: 0 918 2643 4596
Size: 918 1725 1953
Fragments arranged by size:
1953 1725 918
HindIII A'AGCT_T
Cuts at: 0 883 1352 3081 4196 4596
Size: 883 469 1729 1115 400
Fragments arranged by size:
1729 1115 883 469 400
KpnI G_GTAC'C
Cuts at: 0 3389 4596
Size: 3389 1207
MfeI C'AATT_G
Cuts at: 0 1097 1934 3444 3762 4562 4596
Size: 1097 837 1510 318 800 34
Fragments arranged by size:
1510 1097 837 800 318 34
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 570 1598 1782 2559 4596
Size: 232 338 1028 184 777 2037
Fragments arranged by size:
2037 1028 777 338 232 184
MscI TGG'CCA
Cuts at: 0 1232 4596
Size: 1232 3364
MslI CAynn'nnrTG
Cuts at: 0 322 1880 3775 4073 4150 4596
Size: 322 1558 1895 298 77 446
Fragments arranged by size:
1895 1558 446 322 298 77
MspA1I CmG'CkG
Cuts at: 0 3290 4541 4596
Size: 3290 1251 55
Fragments arranged by size:
3290 1251 55
NcoI C'CATG_G
Cuts at: 0 1233 1319 4596
Size: 1233 86 3277
Fragments arranged by size:
3277 1233 86
NdeI CA'TA_TG
Cuts at: 0 839 3969 4596
Size: 839 3130 627
Fragments arranged by size:
3130 839 627
NsiI A_TGCA'T
Cuts at: 0 4293 4596
Size: 4293 303
NspI r_CATG'y
Cuts at: 0 1617 1855 1952 2566 3636 4596
Size: 1617 238 97 614 1070 960
Fragments arranged by size:
1617 1070 960 614 238 97
PciI A'CATG_T
Cuts at: 0 1613 4596
Size: 1613 2983
PflMI CCAn_nnn'nTGG
Cuts at: 0 157 3838 4596
Size: 157 3681 758
Fragments arranged by size:
3681 758 157
PfoI T'CCnGG_A
Cuts at: 0 156 683 2523 3482 4596
Size: 156 527 1840 959 1114
Fragments arranged by size:
1840 1114 959 527 156
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 79 111 4596
Size: 79 32 4485
Fragments arranged by size:
4485 79 32
PpuMI rG'GwC_Cy
Cuts at: 0 412 1249 4342 4596
Size: 412 837 3093 254
Fragments arranged by size:
3093 837 412 254
PspOMI G'GGCC_C
Cuts at: 0 2479 4407 4596
Size: 2479 1928 189
Fragments arranged by size:
2479 1928 189
PstI C_TGCA'G
Cuts at: 0 3258 3476 3717 4596
Size: 3258 218 241 879
Fragments arranged by size:
3258 879 241 218
PvuII CAG'CTG
Cuts at: 0 4541 4596
Size: 4541 55
SacI G_AGCT'C
Cuts at: 0 4172 4596
Size: 4172 424
SapI GCTCTTCn'nnn_
Cuts at: 0 1925 4596
Size: 1925 2671
ScaI AGT'ACT
Cuts at: 0 1929 4528 4596
Size: 1929 2599 68
Fragments arranged by size:
2599 1929 68
SexAI A'CCwGG_T
Cuts at: 0 1279 4596
Size: 1279 3317
SfcI C'TryA_G
Cuts at: 0 91 1040 3254 3472 3551 3713 4178 4430
Size: 91 949 2214 218 79 162 465 252
Cuts at: 4430 4596
Size: 166
Fragments arranged by size:
2214 949 465 252 218 166 162 91
79
SmlI C'TyrA_G
Cuts at: 0 3523 4474 4576 4596
Size: 3523 951 102 20
Fragments arranged by size:
3523 951 102 20
SspI AAT'ATT
Cuts at: 0 3366 3912 4596
Size: 3366 546 684
Fragments arranged by size:
3366 684 546
StuI AGG'CCT
Cuts at: 0 1830 4134 4596
Size: 1830 2304 462
Fragments arranged by size:
2304 1830 462
StyI C'CwwG_G
Cuts at: 0 368 1233 1319 2141 4111 4233 4338 4486
Size: 368 865 86 822 1970 122 105 148
Cuts at: 4486 4596
Size: 110
Fragments arranged by size:
1970 865 822 368 148 122 110 105
86
SwaI ATTT'AAAT
Cuts at: 0 4043 4596
Size: 4043 553
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3409 4596
Size: 3409 1187
TatI w'GTAC_w
Cuts at: 0 1036 1610 1927 1966 4526 4596
Size: 1036 574 317 39 2560 70
Fragments arranged by size:
2560 1036 574 317 70 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 584 616 2198 2230 3445 3477 4596
Size: 584 32 1582 32 1215 32 1119
Fragments arranged by size:
1582 1215 1119 584 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1693 2905 4596
Size: 1693 1212 1691
Fragments arranged by size:
1693 1691 1212
XbaI T'CTAG_A
Cuts at: 0 2239 3513 4596
Size: 2239 1274 1083
Fragments arranged by size:
2239 1274 1083
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2697 4596
Size: 2697 1899
XmnI GAAnn'nnTTC
Cuts at: 0 2324 2426 2492 4596
Size: 2324 102 66 2104
Fragments arranged by size:
2324 2104 102 66
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AleI AlfI AloI
ApaI ApoI AseI BaeI BamHI BanI BanII BbsI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI BseYI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI
BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtsI CspCI
DraI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI Hin4I HincII HindIII KpnI MfeI
MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI
PciI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII
SacI SapI ScaI SexAI SfcI SmlI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AlwNI ApaLI
AscI AsiSI AvaI AvrII BbeI BbvCI BcgI BglI
BmgBI BmtI BplI BsaHI BsaWI BsgI BsiEI BsiWI
BsmBI BspEI BsrBI BsrGI BssHII BssSI BstEII BtgZI
ClaI DrdI EagI EciI FseI FspI FspAI HaeII
HpaI KasI MluI NaeI NarI NgoMIV NheI NotI
NruI PacI PasI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
XhoI XmaI ZraI