Restriction map for
pFN21AB3038
(Linear) (Six-base) MAPSORT of: KIBB3038.seq Check: 5295 from: 1 to: 4203
KIBB3038.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AccI GT'mk_AC
Cuts at: 0 416 1224 4203
Size: 416 808 2979
Fragments arranged by size:
2979 808 416
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 2144 2444 2710 3530 3919 4203
Size: 281 1863 300 266 820 389 284
Fragments arranged by size:
1863 820 389 300 284 281 266
AflII C'TTAA_G
Cuts at: 0 2929 4203
Size: 2929 1274
AhdI GACnn_n'nnGTC
Cuts at: 0 549 4203
Size: 549 3654
AlwNI CAG_nnn'CTG
Cuts at: 0 1476 1615 2272 3670 4203
Size: 1476 139 657 1398 533
Fragments arranged by size:
1476 1398 657 533 139
ApaLI G'TGCA_C
Cuts at: 0 4082 4203
Size: 4082 121
ApoI r'AATT_y
Cuts at: 0 503 617 1565 3558 4203
Size: 503 114 948 1993 645
Fragments arranged by size:
1993 948 645 503 114
AseI AT'TA_AT
Cuts at: 0 2561 4203
Size: 2561 1642
AvrII C'CTAG_G
Cuts at: 0 1071 4203
Size: 1071 3132
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 345 378 4203
Size: 345 33 3825
Fragments arranged by size:
3825 345 33
BamHI G'GATC_C
Cuts at: 0 3036 4203
Size: 3036 1167
BanI G'GyrC_C
Cuts at: 0 196 1306 2178 4203
Size: 196 1110 872 2025
Fragments arranged by size:
2025 1110 872 196
BanII G_rGCy'C
Cuts at: 0 2593 2928 4203
Size: 2593 335 1275
Fragments arranged by size:
2593 1275 335
BbeI G_GCGC'C
Cuts at: 0 200 4203
Size: 200 4003
BbsI GAAGACnn'nnnn_
Cuts at: 0 3877 4203
Size: 3877 326
BbvCI CC'TCA_GC
Cuts at: 0 2333 4203
Size: 2333 1870
BfrBI ATG'CAT
Cuts at: 0 433 756 4203
Size: 433 323 3447
Fragments arranged by size:
3447 433 323
BglII A'GATC_T
Cuts at: 0 768 2604 3079 3594 4203
Size: 768 1836 475 515 609
Fragments arranged by size:
1836 768 609 515 475
BlpI GC'TnA_GC
Cuts at: 0 2676 4203
Size: 2676 1527
Bme1580I G_kGCm'C
Cuts at: 0 4086 4203
Size: 4086 117
BmrI ACTGGGnnnn_n'
Cuts at: 0 226 1997 3042 3528 4203
Size: 226 1771 1045 486 675
Fragments arranged by size:
1771 1045 675 486 226
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 37 1311 3941 4203
Size: 37 1274 2630 262
Fragments arranged by size:
2630 1274 262 37
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1281 1358 1688 1990 2222 2942 3662 4203
Size: 1281 77 330 302 232 720 720 541
Fragments arranged by size:
1281 720 720 541 330 302 232 77
Bpu10I CC'TnA_GC
Cuts at: 0 2333 4203
Size: 2333 1870
BsaI GGTCTCn'nnnn_
Cuts at: 0 1524 3852 4203
Size: 1524 2328 351
Fragments arranged by size:
2328 1524 351
BsaAI yAC'GTr
Cuts at: 0 2911 4203
Size: 2911 1292
BsaHI Gr'CG_yC
Cuts at: 0 109 197 4203
Size: 109 88 4006
Fragments arranged by size:
4006 109 88
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2469 2499 3103 3133 3635 3665 4161 4191
Size: 2469 30 604 30 502 30 496 30
Cuts at: 4191 4203
Size: 12
Fragments arranged by size:
2469 604 502 496 30 30 30 30
12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 145 1273 1900 2088 3111 3231 3234
Size: 118 27 1128 627 188 1023 120 3
Cuts at: 3234 4169 4203
Size: 935 34
Fragments arranged by size:
1128 1023 935 627 188 120 118 34
27 3
BseYI C'CCAG_C
Cuts at: 0 115 481 1752 1997 2412 2671 4203
Size: 115 366 1271 245 415 259 1532
Fragments arranged by size:
1532 1271 415 366 259 245 115
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 582 698 971 1148 4203
Size: 582 116 273 177 3055
Fragments arranged by size:
3055 582 273 177 116
BsiHKAI G_wGCw'C
Cuts at: 0 2928 4086 4203
Size: 2928 1158 117
Fragments arranged by size:
2928 1158 117
BsmI GAATG_Cn'
Cuts at: 0 390 4203
Size: 390 3813
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1662 4203
Size: 1662 2541
Bsp1286I G_dGCh'C
Cuts at: 0 2593 2928 4086 4203
Size: 2593 335 1158 117
Fragments arranged by size:
2593 1158 335 117
BspHI T'CATG_A
Cuts at: 0 186 4203
Size: 186 4017
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1835 1945 4203
Size: 1835 110 2258
Fragments arranged by size:
2258 1835 110
BsrBI CCG'CTC
Cuts at: 0 92 4203
Size: 92 4111
BsrDI GCAATG_nn'
Cuts at: 0 629 3707 3910 3929 4068 4203
Size: 629 3078 203 19 139 135
Fragments arranged by size:
3078 629 203 139 135 19
BsrGI T'GTAC_A
Cuts at: 0 1032 2878 4203
Size: 1032 1846 1325
Fragments arranged by size:
1846 1325 1032
BstAPI GCAn_nnn'nTGC
Cuts at: 0 340 4203
Size: 340 3863
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2014 4203
Size: 2014 2189
BstYI r'GATC_y
Cuts at: 0 768 837 2604 3036 3079 3594 3852 4203
Size: 768 69 1767 432 43 515 258 351
Fragments arranged by size:
1767 768 515 432 351 258 69 43
Bsu36I CC'TnA_GG
Cuts at: 0 1103 2115 4203
Size: 1103 1012 2088
Fragments arranged by size:
2088 1103 1012
BtgI C'CryG_G
Cuts at: 0 1001 1044 1891 2289 3806 4203
Size: 1001 43 847 398 1517 397
Fragments arranged by size:
1517 1001 847 398 397 43
BtsI GCAGTG_nn'
Cuts at: 0 1594 2105 2219 3761 4203
Size: 1594 511 114 1542 442
Fragments arranged by size:
1594 1542 511 442 114
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1329 1364 4203
Size: 1329 35 2839
Fragments arranged by size:
2839 1329 35
DraI TTT'AAA
Cuts at: 0 560 1460 3237 4203
Size: 560 900 1777 966
Fragments arranged by size:
1777 966 900 560
EaeI y'GGCC_r
Cuts at: 0 849 1047 1617 1700 4203
Size: 849 198 570 83 2503
Fragments arranged by size:
2503 849 570 198 83
EarI CTCTTCn'nnn_
Cuts at: 0 1986 2454 4203
Size: 1986 468 1749
Fragments arranged by size:
1986 1749 468
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 164 3978 4182 4203
Size: 164 3814 204 21
Fragments arranged by size:
3814 204 164 21
EcoICRI GAG'CTC
Cuts at: 0 2926 4203
Size: 2926 1277
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 1281 1358 1688 1990 2144 2222 2444
Size: 281 1000 77 330 302 154 78 222
Cuts at: 2444 2710 2942 3530 3662 3919 4203
Size: 266 232 588 132 257 284
Fragments arranged by size:
1000 588 330 302 284 281 266 257
232 222 154 132 78 77
EcoO109I rG'GnC_Cy
Cuts at: 0 210 1957 3229 4143 4203
Size: 210 1747 1272 914 60
Fragments arranged by size:
1747 1272 914 210 60
EcoRI G'AATT_C
Cuts at: 0 1565 4203
Size: 1565 2638
HaeII r_GCGC'y
Cuts at: 0 145 200 4203
Size: 145 55 4003
Fragments arranged by size:
4003 145 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 94 126 535 567 645 677 690 722
Size: 94 32 409 32 78 32 13 32
Cuts at: 722 2287 2319 3175 3207 3635 3667 4203
Size: 1565 32 856 32 428 32 536
Fragments arranged by size:
1565 856 536 428 409 94 78 32
32 32 32 32 32 32 13
HincII GTy'rAC
Cuts at: 0 585 2619 3465 4203
Size: 585 2034 846 738
Fragments arranged by size:
2034 846 738 585
HindIII A'AGCT_T
Cuts at: 0 677 3859 4203
Size: 677 3182 344
Fragments arranged by size:
3182 677 344
HpaI GTT'AAC
Cuts at: 0 2619 3465 4203
Size: 2619 846 738
Fragments arranged by size:
2619 846 738
KasI G'GCGC_C
Cuts at: 0 196 4203
Size: 196 4007
MfeI C'AATT_G
Cuts at: 0 1036 1341 3092 4203
Size: 1036 305 1751 1111
Fragments arranged by size:
1751 1111 1036 305
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 2723 4203
Size: 730 1993 1480
Fragments arranged by size:
1993 1480 730
MscI TGG'CCA
Cuts at: 0 851 1049 1619 1702 4203
Size: 851 198 570 83 2501
Fragments arranged by size:
2501 851 570 198 83
MslI CAynn'nnrTG
Cuts at: 0 191 428 438 641 1049 4203
Size: 191 237 10 203 408 3154
Fragments arranged by size:
3154 408 237 203 191 10
MspA1I CmG'CkG
Cuts at: 0 37 1491 1585 2014 2416 4203
Size: 37 1454 94 429 402 1787
Fragments arranged by size:
1787 1454 429 402 94 37
NarI GG'CG_CC
Cuts at: 0 197 4203
Size: 197 4006
NcoI C'CATG_G
Cuts at: 0 1001 1044 2289 3806 4203
Size: 1001 43 1245 1517 397
Fragments arranged by size:
1517 1245 1001 397 43
NdeI CA'TA_TG
Cuts at: 0 2987 4203
Size: 2987 1216
NsiI A_TGCA'T
Cuts at: 0 435 758 4203
Size: 435 323 3445
Fragments arranged by size:
3445 435 323
NspI r_CATG'y
Cuts at: 0 1360 2699 4203
Size: 1360 1339 1504
Fragments arranged by size:
1504 1360 1339
PasI CC'CwG_GG
Cuts at: 0 1687 4203
Size: 1687 2516
PfoI T'CCnGG_A
Cuts at: 0 3128 3820 4203
Size: 3128 692 383
Fragments arranged by size:
3128 692 383
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1961 1993 4203
Size: 1961 32 2210
Fragments arranged by size:
2210 1961 32
PpuMI rG'GwC_Cy
Cuts at: 0 1957 3229 4143 4203
Size: 1957 1272 914 60
Fragments arranged by size:
1957 1272 914 60
PsiI TTA'TAA
Cuts at: 0 428 4203
Size: 428 3775
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3794 3826 4203
Size: 3794 32 377
Fragments arranged by size:
3794 377 32
PstI C_TGCA'G
Cuts at: 0 1059 1722 1832 3939 4203
Size: 1059 663 110 2107 264
Fragments arranged by size:
2107 1059 663 264 110
PvuII CAG'CTG
Cuts at: 0 1491 1585 2014 2416 4203
Size: 1491 94 429 402 1787
Fragments arranged by size:
1787 1491 429 402 94
SacI G_AGCT'C
Cuts at: 0 2928 4203
Size: 2928 1275
SexAI A'CCwGG_T
Cuts at: 0 2307 3429 4203
Size: 2307 1122 774
Fragments arranged by size:
2307 1122 774
SfcI C'TryA_G
Cuts at: 0 357 764 1055 1225 1718 1734 1828 3935
Size: 357 407 291 170 493 16 94 2107
Cuts at: 3935 3990 4203
Size: 55 213
Fragments arranged by size:
2107 493 407 357 291 213 170 94
55 16
SfoI GGC'GCC
Cuts at: 0 198 4203
Size: 198 4005
SmlI C'TyrA_G
Cuts at: 0 2929 4203
Size: 2929 1274
SphI G_CATG'C
Cuts at: 0 2699 4203
Size: 2699 1504
SspI AAT'ATT
Cuts at: 0 3261 3474 4203
Size: 3261 213 729
Fragments arranged by size:
3261 729 213
StuI AGG'CCT
Cuts at: 0 1018 3118 4203
Size: 1018 2100 1085
Fragments arranged by size:
2100 1085 1018
StyI C'CwwG_G
Cuts at: 0 497 791 827 1001 1044 1071 2289 3806
Size: 497 294 36 174 43 27 1218 1517
Cuts at: 3806 4203
Size: 397
Fragments arranged by size:
1517 1218 497 397 294 174 43 36
27
SwaI ATTT'AAAT
Cuts at: 0 1460 4203
Size: 1460 2743
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2426 4203
Size: 2426 1777
TatI w'GTAC_w
Cuts at: 0 1032 1377 1759 2878 4065 4130 4203
Size: 1032 345 382 1119 1187 65 73
Fragments arranged by size:
1187 1119 1032 382 345 73 65
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1346 1378 4203
Size: 1346 32 2825
Fragments arranged by size:
2825 1346 32
Tth111I GACn'n_nGTC
Cuts at: 0 2395 4203
Size: 2395 1808
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2370 4203
Size: 2370 1833
XmnI GAAnn'nnTTC
Cuts at: 0 3535 4203
Size: 3535 668
Enzymes that do cut:
AccI AcuI AflII AhdI AlwNI ApaLI ApoI AseI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI
BstAPI BstXI BstYI Bsu36I BtgI BtsI CspCI DraI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII
Hin4I HincII HindIII HpaI KasI MfeI MmeI MscI
MslI MspA1I NarI NcoI NdeI NsiI NspI PasI
PfoI PpiI PpuMI PsiI PsrI PstI PvuII SacI
SexAI SfcI SfoI SmlI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AleI
AlfI AloI ApaI AscI AsiSI AvaI BcgI BciVI
BclI BglI BmgBI BmtI BpuEI BsaBI BsaWI BsiEI
BsiWI BspEI BsrFI BssHII BssSI BstBI BstEII BstZ17I
BtgZI ClaI DraIII DrdI EagI EcoNI EcoRV FalI
FseI FspI FspAI KpnI MluI NaeI NgoMIV NheI
NotI NruI PacI PciI PflMI PmeI PmlI PshAI
PspOMI PspXI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI XbaI XhoI XmaI ZraI