Restriction map for
pFN21AB1755
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# NO CUT ENZYMES ( 61 enzymes) #
##################################
AccI AclI AflII AocI AscI
AvrII BamHI BbeI BbrPI BclI
BfrI BlnI BsaBI BsiWI BspLU11I
Bst1107I BstBI BstZ17I Bsu36I CpoI
EagI EclXI Eco52I Eco81I EcoRV
FbaI FseI HpaI KasI KspI
MamI MfeI MunI NarI NheI
NotI NruI NspI NspV PacI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SmaI SnaBI SpeI
SphI Sse8387I SwaI XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AhdI 2962 GACGGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 305 AGCGCT AGC^GCT
ApaI 1142 GGGCCC GGGCC^C
AseI 3151 ATTAAT AT^TAAT
AsnI 3151 ATTAAT AT^TAAT
Asp700I 987 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2962 GACGGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1189 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1064 TGCGCA TGC^GCA
BalI 689 TGGCCA TGG^CCA
BciVI 1993 GGATAC GTATCC(6/5)
BfaI 3307 CTAG C^TAG
BglI 4378 GCCTCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3809 AGATCT A^GATCT
BsaAI 1933 TACGTG [CT]AC^GT[GA]
BsiEI 151 CGGTCG CG[GA][CT]^CG
BsmI 3325 GCATTC GAATGC(1/-1)
BssHII 936 GCGCGC G^CGCGC
BssSI 2272 CACGAG CACGAG(-5/-1)
BstAPI 2665 GCAGTCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 2823 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2962 GACGGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 305 AGCGCT AGC^GCT
EcoRI 3985 GAATTC G^AATTC
EcoT22I 3323 ATGCAT ATGCA^T
FspI 1064 TGCGCA TGC^GCA
HindIII 3962 AAGCTT A^AGCTT
MaeI 3307 CTAG C^TAG
MluI 2810 ACGCGT A^CGCGT
MluNI 689 TGGCCA TGG^CCA
MscI 689 TGGCCA TGG^CCA
NaeI 2371 GCCGGC GCC^GGC
NcoI 1157 CCATGG C^CATGG
NdeI 3826 CATATG CA^TATG
NgoMIV 2371 GCCGGC G^CCGGC
NsiI 3323 ATGCAT ATGCA^T
PaeR7I 3001 CTCGAG C^TCGAG
PflFI 1189 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3151 ATTAAT AT^TAAT
ScaI 3401 AGTACT AGT^ACT
SexAI 2824 ACCTGGT A^CC[AT]GGT
SspI 64 AATATT AAT^ATT
Tth111I 1189 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3001 CTCGAG C^TCGAG
XmnI 987 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 2913 TCCGGA T^CCGGA
3955 TCCGGA
AgeI 1305 ACCGGT A^CCGGT
1543 ACCGGT
AlwNI 204 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
268 CAGTCCCTG
BlpI 3112 GCTGAGC GC^T[ACGT]AGC
4158 GCTCAGC
Bpu1102I 3112 GCTGAGC GC^T[ACGT]AGC
4158 GCTCAGC
BseAI 2913 TCCGGA T^CCGGA
3955 TCCGGA
Bsp1407I 1055 TGTACA T^GTACA
4304 TGTACA
BspDI 804 ATCGAT AT^CGAT
3233 ATCGAT
BspEI 2913 TCCGGA T^CCGGA
3955 TCCGGA
BspHI 3276 TCATGA T^CATGA
3755 TCATGA
BsrGI 1055 TGTACA T^GTACA
4304 TGTACA
BstEII 855 GGTCACC G^GT[ACGT]ACC
4059 GGTGACC
BstPI 855 GGTCACC G^GT[ACGT]ACC
4059 GGTGACC
BstXI 542 CCATGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2136 CCATCCTTATGG
CelII 3112 GCTGAGC GC^T[ACGT]AGC
4158 GCTCAGC
ClaI 804 ATCGAT AT^CGAT
3233 ATCGAT
DraI 2613 TTTAAA TTT^AAA
2866 TTTAAA
EcoNI 860 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1032 CCTGCACAAGG
EcoO65I 855 GGTCACC G^GT[ACGT]ACC
4059 GGTGACC
HaeII 305 AGCGCT [GA]GCGC^[CT]
1099 AGCGCC
HincII 1675 GTCAAC GT[CT]^[GA]AC
2362 GTCAAC
HindII 1675 GTCAAC GT[CT]^[GA]AC
2362 GTCAAC
MroI 2913 TCCGGA T^CCGGA
3955 TCCGGA
PinAI 1305 ACCGGT A^CCGGT
1543 ACCGGT
RcaI 3276 TCATGA T^CATGA
3755 TCATGA
SacI 2998 GAGCTC GAGCT^C
3550 GAGCTC
SapI 439 GAAGAGC GCTCTTC(1/4)
2995 GAAGAGC
SgrAI 1304 CACCGGTG C[GA]^CCGG[CT]G
1542 CACCGGTG
SspBI 1055 TGTACA T^GTACA
4304 TGTACA
StuI 1010 AGGCCT AGG^CCT
2417 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AatII Acc65I AccII AciI AcsI
AcyI AfaI AflIII AluI Alw44I
AlwI ApaLI ApoI Asp718I AspHI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcgI BcnI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PshAI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII