(Circular) (Six-base) MAPSORT of: pFN21AB0045.seq Check: 3576 from: 1 to: 9004 pFN21AB0045 9004 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6157 6157 Size: 9004 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6814 278 Size: 53 83 186 936 5278 2468 Fragments arranged by size: 5278 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3880 6347 1937 Size: 1943 2467 4594 Fragments arranged by size: 4594 2467 1943 Acc65I G'GTAC_C Cuts at: 4223 6325 4223 Size: 2102 6902 AclI AA'CG_TT Cuts at: 1563 2644 7131 7504 1563 Size: 1081 4487 373 3063 Fragments arranged by size: 4487 3063 1081 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4110 5037 6153 7067 8451 8719 1632 Size: 2478 927 1116 914 1384 268 1917 Fragments arranged by size: 2478 1917 1384 1116 927 914 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8807 AflIII A'CryG_T Cuts at: 1203 3875 5069 7903 8880 1203 Size: 2672 1194 2834 977 1327 Fragments arranged by size: 2834 2672 1327 1194 977 AgeI A'CCGG_T Cuts at: 7835 7835 Size: 9004 AhdI GACnn_n'nnGTC Cuts at: 7733 7733 Size: 9004 AleI CACnn'nnGTG Cuts at: 1166 2623 4994 1166 Size: 1457 2371 5176 Fragments arranged by size: 5176 2371 1457 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2910 1184 Size: 1726 7278 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5450 5482 5450 Size: 32 8972 AlwNI CAG_nnn'CTG Cuts at: 2141 2639 2816 2819 4355 6747 8319 2141 Size: 498 177 3 1536 2392 1572 2826 Fragments arranged by size: 2826 2392 1572 1536 498 177 3 ApaLI G'TGCA_C Cuts at: 2895 7061 8217 2895 Size: 4166 1156 3682 Fragments arranged by size: 4166 3682 1156 ApoI r'AATT_y Cuts at: 1493 2413 2737 2985 3013 3179 3289 3581 4031 Size: 920 324 248 28 166 110 292 450 Cuts at: 4031 4244 4589 4645 4652 4730 4757 5287 5311 Size: 213 345 56 7 78 27 530 24 Cuts at: 5311 6085 6136 6313 6539 6691 8800 1493 Size: 774 51 177 226 152 2109 1697 Fragments arranged by size: 2109 1697 920 774 530 450 345 324 292 248 226 213 177 166 152 110 78 56 51 28 27 24 7 AseI AT'TA_AT Cuts at: 160 1032 7558 160 Size: 872 6526 1606 Fragments arranged by size: 6526 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9004 AvaI C'yCGr_G Cuts at: 1870 1943 2011 4430 6175 6329 1870 Size: 73 68 2419 1745 154 4545 Fragments arranged by size: 4545 2419 1745 154 73 68 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4944 4977 5670 5703 4944 Size: 33 693 33 8245 Fragments arranged by size: 8245 693 33 33 BamHI G'GATC_C Cuts at: 5780 6334 6701 5780 Size: 554 367 8083 Fragments arranged by size: 8083 554 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2857 4223 5873 6325 7780 Size: 325 217 627 1070 1366 1650 452 1455 Cuts at: 7780 618 Size: 1842 Fragments arranged by size: 1842 1650 1455 1366 1070 627 452 325 217 BanII G_rGCy'C Cuts at: 1391 2285 4150 5404 6323 1391 Size: 894 1865 1254 919 4072 Fragments arranged by size: 4072 1865 1254 919 894 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2875 3194 4311 928 Size: 971 976 319 1117 5621 Fragments arranged by size: 5621 1117 976 971 319 BbvCI CC'TCA_GC Cuts at: 5809 6157 5809 Size: 348 8656 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7195 7229 1903 Size: 34 5258 34 3678 Fragments arranged by size: 5258 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6896 8106 6896 Size: 1210 7794 BclI T'GATC_A Cuts at: 1548 5422 1548 Size: 3874 5130 BfrBI ATG'CAT Cuts at: 3912 3912 Size: 9004 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2164 7615 136 Size: 107 122 71 631 605 492 5451 1525 Fragments arranged by size: 5451 1525 631 605 492 122 107 71 BglII A'GATC_T Cuts at: 8999 8999 Size: 9004 BlpI GC'TnA_GC Cuts at: 2883 3759 6421 2883 Size: 876 2662 5466 Fragments arranged by size: 5466 2662 876 Bme1580I G_kGCm'C Cuts at: 1790 2899 7065 8221 1790 Size: 1109 4166 1156 2573 Fragments arranged by size: 4166 2573 1156 1109 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9004 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3773 4847 6735 Size: 416 53 83 399 24 2356 1074 1888 Cuts at: 6735 7693 442 Size: 958 1753 Fragments arranged by size: 2356 1888 1753 1074 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9004 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2299 5164 2299 Size: 2865 6139 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3345 3410 3869 4416 6186 7664 1239 Size: 2106 65 459 547 1770 1478 2579 Fragments arranged by size: 2579 2106 1770 1478 547 459 65 Bpu10I CC'TnA_GC Cuts at: 2313 5809 6157 2313 Size: 3496 348 5160 Fragments arranged by size: 5160 3496 348 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3504 5628 7129 7994 8292 3504 Size: 2124 1501 865 298 4216 Fragments arranged by size: 4216 2124 1501 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7667 882 Size: 617 6168 2219 Fragments arranged by size: 6168 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6795 493 Size: 711 5591 2702 Fragments arranged by size: 5591 2702 711 BsaBI GATnn'nnATC Cuts at: 3041 5908 8998 3041 Size: 2867 3090 3047 Fragments arranged by size: 3090 3047 2867 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6811 7193 7828 275 Size: 53 83 186 936 5278 382 635 1451 Fragments arranged by size: 5278 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2052 7437 7835 8109 8256 8667 2052 Size: 5385 398 274 147 411 2389 Fragments arranged by size: 5385 2389 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3481 3511 4963 4993 1178 Size: 30 165 30 2078 30 1452 30 5189 Fragments arranged by size: 5189 2078 1452 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2037 2291 2997 3009 3407 3410 4567 1186 Size: 851 254 706 12 398 3 1157 5623 Fragments arranged by size: 5623 1157 851 706 398 254 12 3 BseYI C'CCAG_C Cuts at: 3851 4440 6162 8207 8703 3851 Size: 589 1722 2045 496 4152 Fragments arranged by size: 4152 2045 1722 589 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3235 4192 4484 5451 5934 1163 Size: 573 1499 957 292 967 483 4233 Fragments arranged by size: 4233 1499 967 957 573 483 292 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6454 7215 8243 1540 Size: 275 186 4453 761 1028 2301 Fragments arranged by size: 4453 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2285 2899 5404 7065 7150 8221 2285 Size: 614 2505 1661 85 1071 3068 Fragments arranged by size: 3068 2505 1661 1071 614 85 BsmI GAATG_Cn' Cuts at: 2118 2468 2693 6515 6608 2118 Size: 350 225 3822 93 4514 Fragments arranged by size: 4514 3822 350 225 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3346 3522 3346 Size: 176 8828 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2285 2899 4150 5404 6323 7065 7150 Size: 399 495 614 1251 1254 919 742 85 Cuts at: 7150 8221 1391 Size: 1071 2174 Fragments arranged by size: 2174 1254 1251 1071 919 742 614 495 399 85 BspEI T'CCGG_A Cuts at: 2052 8667 2052 Size: 6615 2389 BspHI T'CATG_A Cuts at: 6893 8623 6893 Size: 1730 7274 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4363 4928 6157 6359 844 Size: 3519 565 1229 202 3489 Fragments arranged by size: 3519 3489 1229 565 202 BsrBI CCG'CTC Cuts at: 1394 1823 6891 1394 Size: 429 5068 3507 Fragments arranged by size: 5068 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2612 7499 7673 65 Size: 1183 1364 4887 174 1396 Fragments arranged by size: 4887 1396 1364 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7648 7835 1700 Size: 110 5838 187 2869 Fragments arranged by size: 5838 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9004 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9004 BssSI C'ACGA_G Cuts at: 7064 8076 7064 Size: 1012 7992 BstAPI GCAn_nnn'nTGC Cuts at: 794 2816 5819 5837 794 Size: 2022 3003 18 3961 Fragments arranged by size: 3961 3003 2022 18 BstBI TT'CG_AA Cuts at: 4562 7808 4562 Size: 3246 5758 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9004 BstXI CCAn_nnnn'nTGG Cuts at: 2625 4492 2625 Size: 1867 7137 BstYI r'GATC_y Cuts at: 1972 2049 4262 5780 5903 6334 6701 7086 7103 Size: 77 2213 1518 123 431 367 385 17 Cuts at: 7103 8555 8641 8653 8999 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2213 1977 1518 1452 431 385 367 346 123 86 77 17 12 Bsu36I CC'TnA_GG Cuts at: 4228 4228 Size: 9004 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 7513 Fragments arranged by size: 7513 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3717 487 Size: 683 56 2491 5774 Fragments arranged by size: 5774 2491 683 56 BtsI GCAGTG_nn' Cuts at: 2667 3452 3872 5604 6520 7314 7334 2667 Size: 785 420 1732 916 794 20 4337 Fragments arranged by size: 4337 1732 916 794 785 420 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9004 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8969 DraI TTT'AAA Cuts at: 3154 3580 3846 4036 4534 4651 4729 4756 6308 Size: 426 266 190 498 117 78 27 1552 Cuts at: 6308 3154 Size: 5850 Fragments arranged by size: 5850 1552 498 426 266 190 117 78 27 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8011 809 Size: 507 6695 1802 Fragments arranged by size: 6695 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2237 6451 7340 Size: 54 1425 50 275 18 407 4214 889 Cuts at: 7340 8969 8 Size: 1629 43 Fragments arranged by size: 4214 1629 1425 889 407 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6451 1537 Size: 275 4639 4090 Fragments arranged by size: 4639 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2400 4615 5184 5247 5533 6250 6934 1353 Size: 1047 2215 569 63 286 717 684 3423 Fragments arranged by size: 3423 2215 1047 717 684 569 286 63 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7591 7965 8111 387 Size: 1064 138 6002 374 146 1280 Fragments arranged by size: 6002 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2283 5402 2283 Size: 3119 5885 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3345 3410 3869 4110 4416 5037 6153 Size: 393 1713 65 459 241 306 621 1116 Cuts at: 6153 6186 7067 7664 8451 8719 1239 Size: 33 881 597 787 268 1524 Fragments arranged by size: 1713 1524 1116 881 787 621 597 459 393 306 268 241 65 33 EcoNI CCTnn'n_nnAGG Cuts at: 2501 4395 2501 Size: 1894 7110 EcoRI G'AATT_C Cuts at: 2737 2985 3013 3179 5287 5311 6313 6691 2737 Size: 248 28 166 2108 24 1002 378 5050 Fragments arranged by size: 5050 2108 1002 378 248 166 28 24 EcoRV GAT'ATC Cuts at: 3043 3043 Size: 9004 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5035 5035 Size: 9004 FspI TGC'GCA Cuts at: 7510 7510 Size: 9004 HaeII r_GCGC'y Cuts at: 1678 8151 1678 Size: 6473 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2507 2531 2539 2563 2942 Size: 32 135 32 935 24 8 24 379 Cuts at: 2942 2974 4685 4717 5348 5380 7645 7677 7719 Size: 32 1711 32 631 32 2265 32 42 Cuts at: 7719 7751 1373 Size: 32 2626 Fragments arranged by size: 2626 2265 1711 935 631 379 135 42 32 32 32 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 2382 5267 6348 6594 669 Size: 988 281 444 2885 1081 246 3079 Fragments arranged by size: 3079 2885 1081 988 444 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9004 HpaI GTT'AAC Cuts at: 2382 6594 2382 Size: 4212 4792 KpnI G_GTAC'C Cuts at: 4227 6329 4227 Size: 2102 6902 MfeI C'AATT_G Cuts at: 2475 6603 2475 Size: 4128 4876 MluI A'CGCG_T Cuts at: 8880 8880 Size: 9004 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2008 2406 8118 8302 1305 Size: 703 398 5712 184 2007 Fragments arranged by size: 5712 2007 703 398 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2239 10 Size: 54 1425 343 407 6775 Fragments arranged by size: 6775 1425 407 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2535 2623 3865 4994 6962 7321 7480 Size: 648 1369 88 1242 1129 1968 359 159 Cuts at: 7480 518 Size: 2042 Fragments arranged by size: 2042 1968 1369 1242 1129 648 359 159 88 MspA1I CmG'CkG Cuts at: 1606 2205 2816 4206 5420 5684 6420 7097 8245 Size: 599 611 1390 1214 264 736 677 1148 Cuts at: 8245 8490 1606 Size: 245 2120 Fragments arranged by size: 2120 1390 1214 1148 736 677 611 599 264 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 7513 Fragments arranged by size: 7513 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9004 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9004 NotI GC'GGCC_GC Cuts at: 6451 6451 Size: 9004 NsiI A_TGCA'T Cuts at: 3914 3914 Size: 9004 NspI r_CATG'y Cuts at: 1132 1229 3879 4500 5073 6362 7907 1132 Size: 97 2650 621 573 1289 1545 2229 Fragments arranged by size: 2650 2229 1545 1289 621 573 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9004 PciI A'CATG_T Cuts at: 3875 5069 7903 3875 Size: 1194 2834 4976 Fragments arranged by size: 4976 2834 1194 PflMI CCAn_nnn'nTGG Cuts at: 1108 5834 5858 5900 1108 Size: 4726 24 42 4212 Fragments arranged by size: 4726 4212 42 24 PfoI T'CCnGG_A Cuts at: 2785 5707 2785 Size: 2922 6082 PmeI GTTT'AAAC Cuts at: 6308 6308 Size: 9004 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7073 7105 8586 8618 7073 Size: 32 1481 32 7459 Fragments arranged by size: 7459 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9004 PsiI TTA'TAA Cuts at: 3713 6574 3713 Size: 2861 6143 PstI C_TGCA'G Cuts at: 830 3307 4939 5779 6154 6356 830 Size: 2477 1632 840 375 202 3478 Fragments arranged by size: 3478 2477 1632 840 375 202 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9004 PvuII CAG'CTG Cuts at: 2205 2816 4206 5420 5684 2205 Size: 611 1390 1214 264 5525 Fragments arranged by size: 5525 1390 1214 611 264 SacI G_AGCT'C Cuts at: 2285 5404 2285 Size: 3119 5885 SalI G'TCGA_C Cuts at: 1936 6346 1936 Size: 4410 4594 SapI GCTCTTCn'nnn_ Cuts at: 5247 5247 Size: 9004 SbfI CC_TGCA'GG Cuts at: 6356 6356 Size: 9004 ScaI AGT'ACT Cuts at: 2356 3220 3697 5413 7252 8964 2356 Size: 864 477 1716 1839 1712 2396 Fragments arranged by size: 2396 1839 1716 1712 864 477 SfcI C'TryA_G Cuts at: 826 1045 3303 4935 5415 5775 6150 6352 7487 Size: 219 2258 1632 480 360 375 202 1135 Cuts at: 7487 8168 8359 826 Size: 681 191 1471 Fragments arranged by size: 2258 1632 1471 1135 681 480 375 360 219 202 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9004 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9004 SmaI CCC'GGG Cuts at: 1872 6331 1872 Size: 4459 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3519 5643 7108 8009 8271 820 Size: 197 926 1576 2124 1465 901 262 1553 Fragments arranged by size: 2124 1576 1553 1465 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6795 493 Size: 6302 2702 SpeI A'CTAG_T Cuts at: 152 2351 5408 152 Size: 2199 3057 3748 Fragments arranged by size: 3748 3057 2199 SphI G_CATG'C Cuts at: 1132 6362 1132 Size: 5230 3774 SspI AAT'ATT Cuts at: 5 52 2152 2290 2937 6928 5 Size: 47 2100 138 647 3991 2081 Fragments arranged by size: 3991 2100 2081 647 138 47 StuI AGG'CCT Cuts at: 1517 2389 1517 Size: 872 8132 StyI C'CwwG_G Cuts at: 513 1065 2004 5026 6443 513 Size: 552 939 3022 1417 3074 Fragments arranged by size: 3074 3022 1417 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3520 3955 7031 7048 7201 7386 7831 Size: 1255 1940 435 3076 17 153 185 445 Cuts at: 7831 325 Size: 1498 Fragments arranged by size: 3076 1940 1498 1255 445 435 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2354 3218 3619 Size: 274 80 33 51 1443 377 864 401 Cuts at: 3619 3695 5411 5480 7250 8962 96 Size: 76 1716 69 1770 1712 138 Fragments arranged by size: 1770 1716 1712 1443 864 401 377 274 138 80 76 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3553 3585 4122 4154 4963 4995 8964 8996 3553 Size: 32 537 32 809 32 3969 32 3561 Fragments arranged by size: 3969 3561 809 537 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5865 5971 1316 Size: 4549 106 4349 Fragments arranged by size: 4549 4349 106 XbaI T'CTAG_A Cuts at: 6340 6340 Size: 9004 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5542 5542 Size: 9004 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9004 XmaI C'CCGG_G Cuts at: 1870 6329 1870 Size: 4459 4545 XmnI GAAnn'nnTTC Cuts at: 2719 7133 2719 Size: 4414 4590 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6812 276 Size: 53 83 186 936 5278 2468 Fragments arranged by size: 5278 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BsiWI BstZ17I DraIII EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI