(Circular) (Six-base) MAPSORT of: pFN21AA2032.seq Check: 8549 from: 1 to: 7537 pFN21AA2032 7537 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2845 2845 Size: 7537 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5347 278 Size: 53 83 186 936 3811 2468 Fragments arranged by size: 3811 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4880 1937 Size: 2943 4594 Acc65I G'GTAC_C Cuts at: 2671 3767 4858 2671 Size: 1096 1091 5350 Fragments arranged by size: 5350 1096 1091 AclI AA'CG_TT Cuts at: 1563 5664 6037 1563 Size: 4101 373 3063 Fragments arranged by size: 4101 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3503 3525 4248 4764 5600 6984 7252 1632 Size: 1871 22 723 516 836 1384 268 1917 Fragments arranged by size: 1917 1871 1384 836 723 516 268 22 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7340 AflIII A'CryG_T Cuts at: 1203 6436 7413 1203 Size: 5233 977 1327 Fragments arranged by size: 5233 1327 977 AgeI A'CCGG_T Cuts at: 6368 6368 Size: 7537 AhdI GACnn_n'nnGTC Cuts at: 6266 6266 Size: 7537 AleI CACnn'nnGTG Cuts at: 1166 3713 1166 Size: 2547 4990 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7537 AlwNI CAG_nnn'CTG Cuts at: 2903 5280 6852 2903 Size: 2377 1572 3588 Fragments arranged by size: 3588 2377 1572 ApaLI G'TGCA_C Cuts at: 5594 6750 5594 Size: 1156 6381 ApoI r'AATT_y Cuts at: 1493 2299 2475 2547 3265 4846 5072 5224 7333 Size: 806 176 72 718 1581 226 152 2109 Cuts at: 7333 1493 Size: 1697 Fragments arranged by size: 2109 1697 1581 806 718 226 176 152 72 AseI AT'TA_AT Cuts at: 160 1032 2880 4178 6091 160 Size: 872 1848 1298 1913 1606 Fragments arranged by size: 1913 1848 1606 1298 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7537 AvaI C'yCGr_G Cuts at: 1870 1943 4423 4624 4862 1870 Size: 73 2480 201 238 4545 Fragments arranged by size: 4545 2480 238 201 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3751 3784 3751 Size: 33 7504 BamHI G'GATC_C Cuts at: 4867 5234 4867 Size: 367 7170 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2671 3767 4435 4858 6313 Size: 325 217 627 884 1096 668 423 1455 Cuts at: 6313 618 Size: 1842 Fragments arranged by size: 1842 1455 1096 884 668 627 423 325 217 BanII G_rGCy'C Cuts at: 1391 2154 3300 3837 4856 1391 Size: 763 1146 537 1019 4072 Fragments arranged by size: 4072 1146 1019 763 537 BbeI G_GCGC'C Cuts at: 4439 4439 Size: 7537 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2461 2748 928 Size: 971 562 287 5717 Fragments arranged by size: 5717 971 562 287 BbvCI CC'TCA_GC Cuts at: 2968 2985 3736 4519 2968 Size: 17 751 783 5986 Fragments arranged by size: 5986 783 751 17 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5728 5762 1903 Size: 34 3791 34 3678 Fragments arranged by size: 3791 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2090 4640 5429 6639 2090 Size: 2550 789 1210 2988 Fragments arranged by size: 2988 2550 1210 789 BclI T'GATC_A Cuts at: 1548 3822 1548 Size: 2274 5263 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6148 136 Size: 107 122 71 631 605 4476 1525 Fragments arranged by size: 4476 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3775 7532 3775 Size: 3757 3780 BlpI GC'TnA_GC Cuts at: 4752 4954 4752 Size: 202 7335 Bme1580I G_kGCm'C Cuts at: 1790 3951 5598 6754 1790 Size: 2161 1647 1156 2573 Fragments arranged by size: 2573 2161 1647 1156 BmgBI CAC'GTC Cuts at: 1318 3711 1318 Size: 2393 5144 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2149 2861 5268 Size: 416 53 83 399 24 732 712 2407 Cuts at: 5268 6226 442 Size: 958 1753 Fragments arranged by size: 2407 1753 958 732 712 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7537 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3585 3979 4482 6197 1239 Size: 2346 394 503 1715 2579 Fragments arranged by size: 2579 2346 1715 503 394 Bpu10I CC'TnA_GC Cuts at: 2968 2985 3736 4519 2968 Size: 17 751 783 5986 Fragments arranged by size: 5986 783 751 17 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4021 4320 5662 6527 6825 4021 Size: 299 1342 865 298 4733 Fragments arranged by size: 4733 1342 865 299 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2968 3995 6200 882 Size: 617 1469 1027 2205 2219 Fragments arranged by size: 2219 2205 1469 1027 617 BsaAI yAC'GTr Cuts at: 493 1204 5328 493 Size: 711 4124 2702 Fragments arranged by size: 4124 2702 711 BsaBI GATnn'nnATC Cuts at: 7531 7531 Size: 7537 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4436 5344 5726 6361 Size: 53 83 186 936 2903 908 382 635 Cuts at: 6361 275 Size: 1451 Fragments arranged by size: 2903 1451 936 908 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5970 6368 6642 6789 7200 5970 Size: 398 274 147 411 6307 Fragments arranged by size: 6307 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3055 3085 3583 3613 3640 Size: 30 165 30 1652 30 498 30 27 Cuts at: 3640 3670 1178 Size: 30 5045 Fragments arranged by size: 5045 1652 498 165 30 30 30 30 30 27 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2388 2802 2956 3451 3724 1186 Size: 1202 414 154 495 273 4999 Fragments arranged by size: 4999 1202 495 414 273 154 BseYI C'CCAG_C Cuts at: 2791 3371 4492 6740 7236 2791 Size: 580 1121 2248 496 3092 Fragments arranged by size: 3092 2248 1121 580 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2526 3021 3189 1163 Size: 573 790 495 168 5511 Fragments arranged by size: 5511 790 573 495 168 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4987 5748 6776 1540 Size: 275 186 2986 761 1028 2301 Fragments arranged by size: 2986 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5598 5683 6754 5598 Size: 85 1071 6381 Fragments arranged by size: 6381 1071 85 BsmI GAATG_Cn' Cuts at: 4523 5048 5141 4523 Size: 525 93 6919 Fragments arranged by size: 6919 525 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3636 3636 Size: 7537 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2154 3300 3837 3951 4856 5598 5683 Size: 399 364 1146 537 114 905 742 85 Cuts at: 5683 6754 1391 Size: 1071 2174 Fragments arranged by size: 2174 1146 1071 905 742 537 399 364 114 85 BspEI T'CCGG_A Cuts at: 7200 7200 Size: 7537 BspHI T'CATG_A Cuts at: 5426 7156 5426 Size: 1730 5807 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2654 2845 2934 2950 4705 4825 4892 844 Size: 1810 191 89 16 1755 120 67 3489 Fragments arranged by size: 3489 1810 1755 191 120 89 67 16 BsrBI CCG'CTC Cuts at: 1394 1823 4547 5424 1394 Size: 429 2724 877 3507 Fragments arranged by size: 3507 2724 877 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2172 3026 3665 4071 6032 6206 65 Size: 1183 924 854 639 406 1961 174 1396 Fragments arranged by size: 1961 1396 1183 924 854 639 406 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3380 4562 6181 6368 1700 Size: 110 1570 1182 1619 187 2869 Fragments arranged by size: 2869 1619 1570 1182 187 110 BsrGI T'GTAC_A Cuts at: 96 3673 96 Size: 3577 3960 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7537 BssSI C'ACGA_G Cuts at: 5597 6609 5597 Size: 1012 6525 BstAPI GCAn_nnn'nTGC Cuts at: 794 2639 2648 794 Size: 1845 9 5683 Fragments arranged by size: 5683 1845 9 BstBI TT'CG_AA Cuts at: 2750 6341 2750 Size: 3591 3946 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7537 BstYI r'GATC_y Cuts at: 1972 3775 4867 5234 5619 5636 7088 7174 7186 Size: 1803 1092 367 385 17 1452 86 12 Cuts at: 7186 7532 1972 Size: 346 1977 Fragments arranged by size: 1977 1803 1452 1092 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3161 3636 513 Size: 552 939 1157 475 4414 Fragments arranged by size: 4414 1157 939 552 475 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6798 Fragments arranged by size: 6798 683 56 BtsI GCAGTG_nn' Cuts at: 3033 3104 3690 4086 4305 5053 5847 5867 3033 Size: 71 586 396 219 748 794 20 4703 Fragments arranged by size: 4703 794 748 586 396 219 71 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7537 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2193 2228 4054 4089 529 Size: 35 1629 35 1826 35 3977 Fragments arranged by size: 3977 1826 1629 35 35 35 DraI TTT'AAA Cuts at: 4014 4841 4014 Size: 827 6710 DraIII CAC_nnn'GTG Cuts at: 2648 3392 2648 Size: 744 6793 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6544 809 Size: 507 5228 1802 Fragments arranged by size: 5228 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4984 5873 7502 Size: 54 1425 50 275 18 3154 889 1629 Cuts at: 7502 8 Size: 43 Fragments arranged by size: 3154 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4984 1537 Size: 275 3172 4090 Fragments arranged by size: 4090 3172 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3244 3522 3538 3816 4098 4416 5467 1353 Size: 1891 278 16 278 282 318 1051 3423 Fragments arranged by size: 3423 1891 1051 318 282 278 278 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6124 6498 6644 387 Size: 1064 138 4535 374 146 1280 Fragments arranged by size: 4535 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3503 3525 3585 3979 4248 4482 4764 Size: 393 1871 22 60 394 269 234 282 Cuts at: 4764 5600 6197 6984 7252 1239 Size: 836 597 787 268 1524 Fragments arranged by size: 1871 1524 836 787 597 394 393 282 269 268 234 60 22 EcoNI CCTnn'n_nnAGG Cuts at: 3147 3147 Size: 7537 EcoO109I rG'GnC_Cy Cuts at: 3113 3152 4340 3113 Size: 39 1188 6310 Fragments arranged by size: 6310 1188 39 EcoRI G'AATT_C Cuts at: 2299 2547 4846 5224 2299 Size: 248 2299 378 4612 Fragments arranged by size: 4612 2299 378 248 EcoRV GAT'ATC Cuts at: 2412 2412 Size: 7537 FspI TGC'GCA Cuts at: 4695 6043 4695 Size: 1348 6189 HaeII r_GCGC'y Cuts at: 1678 4439 4787 6684 1678 Size: 2761 348 1897 2531 Fragments arranged by size: 2761 2531 1897 348 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2000 2032 3583 3615 6178 Size: 32 135 32 428 32 1551 32 2563 Cuts at: 6178 6210 6252 6284 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2563 1551 428 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2801 4881 5127 669 Size: 988 281 863 2080 246 3079 Fragments arranged by size: 3079 2080 988 863 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7537 HpaI GTT'AAC Cuts at: 5127 5127 Size: 7537 KasI G'GCGC_C Cuts at: 4435 4435 Size: 7537 KpnI G_GTAC'C Cuts at: 2675 3771 4862 2675 Size: 1096 1091 5350 Fragments arranged by size: 5350 1096 1091 MfeI C'AATT_G Cuts at: 2079 5136 2079 Size: 3057 4480 MluI A'CGCG_T Cuts at: 7413 7413 Size: 7537 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3360 6651 6835 1305 Size: 2055 3291 184 2007 Fragments arranged by size: 3291 2055 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5715 Fragments arranged by size: 5715 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3713 5495 5854 6013 518 Size: 648 2547 1782 359 159 2042 Fragments arranged by size: 2547 2042 1782 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2903 4199 4241 4454 4953 5630 6778 7023 Size: 1297 1296 42 213 499 677 1148 245 Cuts at: 7023 1606 Size: 2120 Fragments arranged by size: 2120 1297 1296 1148 677 499 245 213 42 NarI GG'CG_CC Cuts at: 4436 4436 Size: 7537 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6046 Fragments arranged by size: 6046 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7537 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7537 NotI GC'GGCC_GC Cuts at: 4984 4984 Size: 7537 NspI r_CATG'y Cuts at: 1132 1229 2633 4895 6440 1132 Size: 97 1404 2262 1545 2229 Fragments arranged by size: 2262 2229 1545 1404 97 PasI CC'CwG_GG Cuts at: 1348 2954 4169 4634 1348 Size: 1606 1215 465 4251 Fragments arranged by size: 4251 1606 1215 465 PciI A'CATG_T Cuts at: 6436 6436 Size: 7537 PflMI CCAn_nnn'nTGG Cuts at: 1108 4055 1108 Size: 2947 4590 PmeI GTTT'AAAC Cuts at: 4841 4841 Size: 7537 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3515 3547 5606 5638 7119 7151 3515 Size: 32 2059 32 1481 32 3901 Fragments arranged by size: 3901 2059 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3113 3152 4340 3113 Size: 39 1188 6310 Fragments arranged by size: 6310 1188 39 PshAI GACnn'nnGTC Cuts at: 1613 4345 1613 Size: 2732 4805 PsiI TTA'TAA Cuts at: 2655 5107 2655 Size: 2452 5085 PstI C_TGCA'G Cuts at: 830 4434 4591 4889 830 Size: 3604 157 298 3478 Fragments arranged by size: 3604 3478 298 157 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7537 PvuII CAG'CTG Cuts at: 2903 4241 4454 2903 Size: 1338 213 5986 Fragments arranged by size: 5986 1338 213 SalI G'TCGA_C Cuts at: 1936 4879 1936 Size: 2943 4594 SapI GCTCTTCn'nnn_ Cuts at: 3244 4098 4416 3244 Size: 854 318 6365 Fragments arranged by size: 6365 854 318 SbfI CC_TGCA'GG Cuts at: 4889 4889 Size: 7537 ScaI AGT'ACT Cuts at: 4006 5785 7497 4006 Size: 1779 1712 4046 Fragments arranged by size: 4046 1779 1712 SfcI C'TryA_G Cuts at: 826 1045 2724 4430 4443 4587 4885 6020 6701 Size: 219 1679 1706 13 144 298 1135 681 Cuts at: 6701 6892 826 Size: 191 1471 Fragments arranged by size: 1706 1679 1471 1135 681 298 219 191 144 13 SfoI GGC'GCC Cuts at: 4437 4437 Size: 7537 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7537 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7537 SmaI CCC'GGG Cuts at: 1872 4425 4626 4864 1872 Size: 2553 201 238 4545 Fragments arranged by size: 4545 2553 238 201 SmlI C'TyrA_G Cuts at: 820 1017 1943 4000 4335 5641 6542 6804 820 Size: 197 926 2057 335 1306 901 262 1553 Fragments arranged by size: 2057 1553 1306 926 901 335 262 197 SnaBI TAC'GTA Cuts at: 493 5328 493 Size: 4835 2702 SpeI A'CTAG_T Cuts at: 152 2254 152 Size: 2102 5435 SphI G_CATG'C Cuts at: 1132 4895 1132 Size: 3763 3774 SspI AAT'ATT Cuts at: 5 52 5461 5 Size: 47 5409 2081 Fragments arranged by size: 5409 2081 47 StuI AGG'CCT Cuts at: 1517 3330 3624 4611 1517 Size: 1813 294 987 4443 Fragments arranged by size: 4443 1813 987 294 StyI C'CwwG_G Cuts at: 513 1065 2004 3325 4976 513 Size: 552 939 1321 1651 3074 Fragments arranged by size: 3074 1651 1321 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3777 4818 5564 5581 5734 5919 6364 Size: 1255 2197 1041 746 17 153 185 445 Cuts at: 6364 325 Size: 1498 Fragments arranged by size: 2197 1498 1255 1041 746 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2516 3059 3673 Size: 274 80 33 51 1443 539 543 614 Cuts at: 3673 4004 5783 7495 96 Size: 331 1779 1712 138 Fragments arranged by size: 1779 1712 1443 614 543 539 331 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3061 3093 4078 4110 4297 4329 7497 7529 3061 Size: 32 985 32 187 32 3168 32 3069 Fragments arranged by size: 3168 3069 985 187 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7537 XbaI T'CTAG_A Cuts at: 4873 4873 Size: 7537 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3604 3604 Size: 7537 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7537 XmaI C'CCGG_G Cuts at: 1870 4423 4624 4862 1870 Size: 2553 201 238 4545 Fragments arranged by size: 4545 2553 238 201 XmnI GAAnn'nnTTC Cuts at: 3022 5666 3022 Size: 2644 4893 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5345 276 Size: 53 83 186 936 3811 2468 Fragments arranged by size: 3811 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI