Restriction map for
pFN21AA2015
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# NO CUT ENZYMES ( 100 enzymes) #
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AatII Acc65I AccI AccIII AclI
AgeI AhdI Alw44I ApaI ApaLI
AscI Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbvCI BciVI
BglI BlnI BlpI BpmI Bpu1102I
BsaI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspHI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DraII DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoO109I
EcoO65I FseI FspI HindIII HpaI
KpnI MluI MroI NaeI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfcI SfiI SgrAI
SmaI SphI Sse8387I StuI SwaI
Tth111I Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AflII 2413 CTTAAG C^TTAAG
AflIII 689 ACATGT A^C[GA][CT]GT
AlwI 111 GATCC GGATC(4/5)
AlwNI 1731 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1530 CCTAAGG CC^T[ACGT]AGG
Aor51HI 152 AGCGCT AGC^GCT
AspHI 48 GAGCTC G[AT]GC[AT]^C
AvaI 1436 CTCGGG C^[CT]CG[GA]G
BanI 370 GGCGCC G^G[CT][GA]CC
BanII 48 GAGCTC G[GA]GC[CT]^C
BbeI 370 GGCGCC GGCGC^C
BbsI 2280 GTCTTC GAAGAC(2/6)
BcgI 708 CGAGGATTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2762 TGATCA T^GATCA
BfrI 2413 CTTAAG C^TTAAG
BglII 1758 AGATCT A^GATCT
BpuAI 2280 GTCTTC GAAGAC(2/6)
BsaAI 671 TACGTA [CT]AC^GT[GA]
BsaBI 279 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1627 GAGGAG GAGGAG(10/8)
BsiHKAI 48 GAGCTC G[AT]GC[AT]^C
BsmBI 24 GAGACG CGTCTC(1/5)
BsmI 1191 GAATGC GAATGC(1/-1)
BsoBI 1436 CTCGGG C^[CT]CG[GA]G
BspLU11I 689 ACATGT A^CATGT
BspMI 167 GCAGGT ACCTGC(4/8)
BsrDI 999 CATTGC GCAATG(2/0)
BstBI 791 TTCGAA TT^CGAA
Bsu36I 1530 CCTAAGG CC^T[ACGT]AGG
DraI 1806 TTTAAA TTT^AAA
Eco47III 152 AGCGCT AGC^GCT
Eco81I 1530 CCTAAGG CC^T[ACGT]AGG
EcoNI 30 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 391 GATATC GAT^ATC
FbaI 2762 TGATCA T^GATCA
KasI 370 GGCGCC G^GCGCC
KspI 140 CCGCGG CCGC^GG
MamI 279 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2178 CAATTG C^AATTG
MunI 2178 CAATTG C^AATTG
NarI 370 GGCGCC GG^CGCC
NspV 791 TTCGAA TT^CGAA
PvuII 1522 CAGCTG CAG^CTG
SacI 48 GAGCTC GAGCT^C
SacII 140 CCGCGG CCGC^GG
SapI 9 GCTCTTC GCTCTTC(1/4)
SfoI 370 GGCGCC GGC^GCC
SfuI 791 TTCGAA TT^CGAA
SnaBI 671 TACGTA TAC^GTA
SpeI 1329 ACTAGT A^CTAGT
XcmI 350 CCATCGTTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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BalI 696 TGGCCA TGG^CCA
2300 TGGCCA
BmyI 48 GAGCTC G[AGT]GC[ACT]^C
227 GTGCCC
BsgI 262 CTGCAC GTGCAG(16/14)
2491 GTGCAG
Bsp1286I 48 GAGCTC G[AGT]GC[ACT]^C
227 GTGCCC
BstYI 110 AGATCC [GA]^GATC[CT]
1758 AGATCT
EcoT22I 1570 ATGCAT ATGCA^T
2156 ATGCAT
FauI 188 CCCGC CCCGC(4/6)
70 GCGGG
HincII 586 GTTGAC GT[CT]^[GA]AC
1640 GTCAAC
HindII 586 GTTGAC GT[CT]^[GA]AC
1640 GTCAAC
MflI 110 AGATCC [GA]^GATC[CT]
1758 AGATCT
MluNI 696 TGGCCA TGG^CCA
2300 TGGCCA
MscI 696 TGGCCA TGG^CCA
2300 TGGCCA
NdeI 541 CATATG CA^TATG
2422 CATATG
NsiI 1570 ATGCAT ATGCA^T
2156 ATGCAT
NspI 579 ACATGC [GA]CATG^[CT]
689 ACATGT
PleI 1121 GACTC GAGTC(4/5)
1915 GAGTC
TfiI 937 GATTC G^A[AT]TC
1258 GAATC
XbaI 1357 TCTAGA T^CTAGA
1764 TCTAGA
XhoII 110 AGATCC [GA]^GATC[CT]
1758 AGATCT
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI ApoI AseI AsnI Asp700I
AvaII BbvI BcnI BfaI BsaHI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1407I BsrGI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PshBI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI SspBI SspI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI