(Circular) (Six-base) MAPSORT of: pFN21AA2015.seq Check: 7517 from: 1 to: 7681 pFN21AA2015 7681 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5491 278 Size: 53 83 186 936 3955 2468 Fragments arranged by size: 3955 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5024 1937 Size: 3087 4594 Acc65I G'GTAC_C Cuts at: 5002 5002 Size: 7681 AclI AA'CG_TT Cuts at: 1563 5808 6181 1563 Size: 4245 373 3063 Fragments arranged by size: 4245 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2002 2874 3276 3812 4032 4272 5744 7128 Size: 370 872 402 536 220 240 1472 1384 Cuts at: 7128 7396 1632 Size: 268 1917 Fragments arranged by size: 1917 1472 1384 872 536 402 370 268 240 220 AfeI AGC'GCT Cuts at: 2159 2159 Size: 7681 AflII C'TTAA_G Cuts at: 820 1017 4418 820 Size: 197 3401 4083 Fragments arranged by size: 4083 3401 197 AflIII A'CryG_T Cuts at: 1203 2694 6580 7557 1203 Size: 1491 3886 977 1327 Fragments arranged by size: 3886 1491 1327 977 AgeI A'CCGG_T Cuts at: 6512 6512 Size: 7681 AhdI GACnn_n'nnGTC Cuts at: 6410 6410 Size: 7681 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7681 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7681 AlwNI CAG_nnn'CTG Cuts at: 3741 5424 6996 3741 Size: 1683 1572 4426 Fragments arranged by size: 4426 1683 1572 ApaLI G'TGCA_C Cuts at: 5738 6894 5738 Size: 1156 6525 ApoI r'AATT_y Cuts at: 1493 2530 2555 2757 2836 2983 3208 3872 4002 Size: 1037 25 202 79 147 225 664 130 Cuts at: 4002 4028 4631 4682 4914 4990 5216 5368 7477 Size: 26 603 51 232 76 226 152 2109 Cuts at: 7477 1493 Size: 1697 Fragments arranged by size: 2109 1697 1037 664 603 232 226 225 202 152 147 130 79 76 51 26 25 AseI AT'TA_AT Cuts at: 160 1032 3726 3791 3954 4049 4739 6235 160 Size: 872 2694 65 163 95 690 1496 1606 Fragments arranged by size: 2694 1606 1496 872 690 163 95 65 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7681 AvaI C'yCGr_G Cuts at: 1870 1943 3441 5006 1870 Size: 73 1498 1565 4545 Fragments arranged by size: 4545 1565 1498 73 BamHI G'GATC_C Cuts at: 5011 5378 5011 Size: 367 7314 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2375 5002 6457 618 Size: 325 217 627 588 2627 1455 1842 Fragments arranged by size: 2627 1842 1455 627 588 325 217 BanII G_rGCy'C Cuts at: 1391 2057 5000 1391 Size: 666 2943 4072 Fragments arranged by size: 4072 2943 666 BbeI G_GCGC'C Cuts at: 2379 2379 Size: 7681 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4278 928 Size: 971 2379 4331 Fragments arranged by size: 4331 2379 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2702 2736 5872 5906 1903 Size: 34 765 34 3136 34 3678 Fragments arranged by size: 3678 3136 765 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5573 6783 5573 Size: 1210 6471 BclI T'GATC_A Cuts at: 1548 4767 1548 Size: 3219 4462 BfrBI ATG'CAT Cuts at: 3577 4163 3577 Size: 586 7095 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6292 136 Size: 107 122 71 631 605 4620 1525 Fragments arranged by size: 4620 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3763 7676 3763 Size: 3913 3768 BlpI GC'TnA_GC Cuts at: 5098 5098 Size: 7681 Bme1580I G_kGCm'C Cuts at: 1790 2236 5742 6898 1790 Size: 446 3506 1156 2573 Fragments arranged by size: 3506 2573 1156 446 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7681 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2110 5412 6370 Size: 416 53 83 399 24 693 3302 958 Cuts at: 6370 442 Size: 1753 Fragments arranged by size: 3302 1753 958 693 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7681 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3509 3509 Size: 7681 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 6341 1239 Size: 5102 2579 Bpu10I CC'TnA_GC Cuts at: 2051 2051 Size: 7681 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2673 3767 4397 5806 6671 6969 2673 Size: 1094 630 1409 865 298 3385 Fragments arranged by size: 3385 1409 1094 865 630 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6344 882 Size: 617 4845 2219 Fragments arranged by size: 4845 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2678 5472 493 Size: 711 1474 2794 2702 Fragments arranged by size: 2794 2702 1474 711 BsaBI GATnn'nnATC Cuts at: 2288 7675 2288 Size: 5387 2294 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2032 2142 2190 2376 Size: 53 83 186 936 499 110 48 186 Cuts at: 2376 5488 5870 6505 275 Size: 3112 382 635 1451 Fragments arranged by size: 3112 1451 936 635 499 382 186 186 110 83 53 48 BsaWI w'CCGG_w Cuts at: 6114 6512 6786 6933 7344 6114 Size: 398 274 147 411 6451 Fragments arranged by size: 6451 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7456 Fragments arranged by size: 7456 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3647 1186 Size: 2461 5220 BseYI C'CCAG_C Cuts at: 6884 7380 6884 Size: 496 7185 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2252 4517 1163 Size: 573 516 2265 4327 Fragments arranged by size: 4327 2265 573 516 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5131 5892 6920 1540 Size: 275 186 3130 761 1028 2301 Fragments arranged by size: 3130 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2057 5742 5827 6898 2057 Size: 3685 85 1071 2840 Fragments arranged by size: 3685 2840 1071 85 BsmI GAATG_Cn' Cuts at: 3202 5192 5285 3202 Size: 1990 93 5598 Fragments arranged by size: 5598 1990 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2023 2023 Size: 7681 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2236 5000 5742 5827 6898 1391 Size: 399 267 179 2764 742 85 1071 2174 Fragments arranged by size: 2764 2174 1071 742 399 267 179 85 BspEI T'CCGG_A Cuts at: 7344 7344 Size: 7681 BspHI T'CATG_A Cuts at: 5570 7300 5570 Size: 1730 5951 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2163 5036 844 Size: 1319 2873 3489 Fragments arranged by size: 3489 2873 1319 BsrBI CCG'CTC Cuts at: 1394 1823 5568 1394 Size: 429 3745 3507 Fragments arranged by size: 3745 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3003 6176 6350 65 Size: 1183 1755 3173 174 1396 Fragments arranged by size: 3173 1755 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6325 6512 1700 Size: 110 4515 187 2869 Fragments arranged by size: 4515 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2329 3523 4354 96 Size: 2233 1194 831 3423 Fragments arranged by size: 3423 2233 1194 831 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7681 BssSI C'ACGA_G Cuts at: 5741 6753 5741 Size: 1012 6669 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7681 BstBI TT'CG_AA Cuts at: 2797 6485 2797 Size: 3688 3993 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7681 BstYI r'GATC_y Cuts at: 1972 2115 3763 5011 5378 5763 5780 7232 7318 Size: 143 1648 1248 367 385 17 1452 86 Cuts at: 7318 7330 7676 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1648 1452 1248 385 367 346 143 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3536 3536 Size: 7681 BtgI C'CryG_G Cuts at: 513 1065 2058 2145 2236 4189 513 Size: 552 993 87 91 1953 4005 Fragments arranged by size: 4005 1953 993 552 91 87 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2987 487 Size: 683 56 1761 5181 Fragments arranged by size: 5181 1761 683 56 BtsI GCAGTG_nn' Cuts at: 2409 3285 4500 4713 4770 5197 5991 6011 2409 Size: 876 1215 213 57 427 794 20 4079 Fragments arranged by size: 4079 1215 876 794 427 213 57 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7681 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3135 3170 529 Size: 35 2571 35 5040 Fragments arranged by size: 5040 2571 35 35 DraI TTT'AAA Cuts at: 3813 4985 3813 Size: 1172 6509 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6688 809 Size: 507 5372 1802 Fragments arranged by size: 5372 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2239 2701 4266 Size: 54 1425 50 275 18 409 462 1565 Cuts at: 4266 4305 4905 5128 6017 7646 8 Size: 39 600 223 889 1629 43 Fragments arranged by size: 1629 1565 1425 889 600 462 409 275 223 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 5128 1537 Size: 275 3316 4090 Fragments arranged by size: 4090 3316 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2021 2337 2689 4104 4653 4827 5611 1353 Size: 668 316 352 1415 549 174 784 3423 Fragments arranged by size: 3423 1415 784 668 549 352 316 174 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6268 6642 6788 387 Size: 1064 138 4679 374 146 1280 Fragments arranged by size: 4679 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2055 2055 Size: 7681 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2002 2874 3276 3812 4032 4272 5744 Size: 393 370 872 402 536 220 240 1472 Cuts at: 5744 6341 7128 7396 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1472 872 787 597 536 402 393 370 268 240 220 EcoNI CCTnn'n_nnAGG Cuts at: 2039 2039 Size: 7681 EcoRI G'AATT_C Cuts at: 3208 4028 4914 4990 5368 3208 Size: 820 886 76 378 5521 Fragments arranged by size: 5521 886 820 378 76 EcoRV GAT'ATC Cuts at: 2398 2398 Size: 7681 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4711 4975 4711 Size: 264 7417 FspI TGC'GCA Cuts at: 6187 6187 Size: 7681 HaeII r_GCGC'y Cuts at: 1678 2048 2161 2379 6828 1678 Size: 370 113 218 4449 2531 Fragments arranged by size: 4449 2531 370 218 113 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2268 2300 3628 3660 4325 Size: 32 135 32 696 32 1328 32 665 Cuts at: 4325 4357 6322 6354 6396 6428 1373 Size: 32 1965 32 42 32 2626 Fragments arranged by size: 2626 1965 1328 696 665 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2593 3647 5025 5271 669 Size: 988 281 655 1054 1378 246 3079 Fragments arranged by size: 3079 1378 1054 988 655 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7681 HpaI GTT'AAC Cuts at: 5271 5271 Size: 7681 KasI G'GCGC_C Cuts at: 2375 2375 Size: 7681 KpnI G_GTAC'C Cuts at: 5006 5006 Size: 7681 MfeI C'AATT_G Cuts at: 4183 5280 4183 Size: 1097 6584 MluI A'CGCG_T Cuts at: 7557 7557 Size: 7681 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2773 4377 6795 6979 1305 Size: 1468 1604 2418 184 2007 Fragments arranged by size: 2418 2007 1604 1468 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2703 4307 10 Size: 54 1425 343 871 1604 3384 Fragments arranged by size: 3384 1604 1425 871 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2241 3008 3582 5639 5998 6157 518 Size: 648 1075 767 574 2057 359 159 2042 Fragments arranged by size: 2057 2042 1075 767 648 574 359 159 MspA1I CmG'CkG Cuts at: 1606 2147 3529 4798 5097 5774 6922 7167 1606 Size: 541 1382 1269 299 677 1148 245 2120 Fragments arranged by size: 2120 1382 1269 1148 677 541 299 245 NarI GG'CG_CC Cuts at: 2376 2376 Size: 7681 NcoI C'CATG_G Cuts at: 513 1065 2058 2236 4189 513 Size: 552 993 178 1953 4005 Fragments arranged by size: 4005 1953 993 552 178 NdeI CA'TA_TG Cuts at: 387 2547 4428 387 Size: 2160 1881 3640 Fragments arranged by size: 3640 2160 1881 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7681 NotI GC'GGCC_GC Cuts at: 5128 5128 Size: 7681 NsiI A_TGCA'T Cuts at: 3579 4165 3579 Size: 586 7095 NspI r_CATG'y Cuts at: 1132 1229 2588 2698 5039 6584 1132 Size: 97 1359 110 2341 1545 2229 Fragments arranged by size: 2341 2229 1545 1359 110 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7681 PciI A'CATG_T Cuts at: 2694 6580 2694 Size: 3886 3795 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7681 PfoI T'CCnGG_A Cuts at: 2178 2364 2534 2178 Size: 186 170 7325 Fragments arranged by size: 7325 186 170 PmeI GTTT'AAAC Cuts at: 4985 4985 Size: 7681 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5750 5782 7263 7295 5750 Size: 32 1481 32 6136 Fragments arranged by size: 6136 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7681 PsiI TTA'TAA Cuts at: 5251 5251 Size: 7681 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4830 4862 4830 Size: 32 7649 PstI C_TGCA'G Cuts at: 830 5033 830 Size: 4203 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7681 PvuII CAG'CTG Cuts at: 3529 3529 Size: 7681 SacI G_AGCT'C Cuts at: 2057 2057 Size: 7681 SacII CC_GC'GG Cuts at: 2148 2148 Size: 7681 SalI G'TCGA_C Cuts at: 1936 5023 1936 Size: 3087 4594 SapI GCTCTTCn'nnn_ Cuts at: 2021 2021 Size: 7681 SbfI CC_TGCA'GG Cuts at: 5033 5033 Size: 7681 ScaI AGT'ACT Cuts at: 5929 7641 5929 Size: 1712 5969 SfcI C'TryA_G Cuts at: 826 1045 5029 6164 6845 7036 826 Size: 219 3984 1135 681 191 1471 Fragments arranged by size: 3984 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2377 2377 Size: 7681 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7681 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7681 SmaI CCC'GGG Cuts at: 1872 5008 1872 Size: 3136 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2688 3746 4376 4418 5785 6686 Size: 197 926 745 1058 630 42 1367 901 Cuts at: 6686 6948 820 Size: 262 1553 Fragments arranged by size: 1553 1367 1058 926 901 745 630 262 197 42 SnaBI TAC'GTA Cuts at: 493 2678 5472 493 Size: 2185 2794 2702 Fragments arranged by size: 2794 2702 2185 SpeI A'CTAG_T Cuts at: 152 3334 152 Size: 3182 4499 SphI G_CATG'C Cuts at: 1132 5039 1132 Size: 3907 3774 SspI AAT'ATT Cuts at: 5 52 2482 3388 4808 5605 5 Size: 47 2430 906 1420 797 2081 Fragments arranged by size: 2430 2081 1420 906 797 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7681 StyI C'CwwG_G Cuts at: 513 1065 2058 2236 4189 5120 513 Size: 552 993 178 1953 931 3074 Fragments arranged by size: 3074 1953 993 931 552 178 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5708 5725 5878 6063 6508 325 Size: 1255 4128 17 153 185 445 1498 Fragments arranged by size: 4128 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2329 2626 3300 Size: 274 80 33 51 1443 352 297 674 Cuts at: 3300 3523 4354 5927 7639 96 Size: 223 831 1573 1712 138 Fragments arranged by size: 1712 1573 1443 831 674 352 297 274 223 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7641 7673 7641 Size: 32 7649 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7681 XbaI T'CTAG_A Cuts at: 3362 3769 5017 3362 Size: 407 1248 6026 Fragments arranged by size: 6026 1248 407 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2362 2362 Size: 7681 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7681 XmaI C'CCGG_G Cuts at: 1870 5006 1870 Size: 3136 4545 XmnI GAAnn'nnTTC Cuts at: 2015 3765 4323 5810 2015 Size: 1750 558 1487 3886 Fragments arranged by size: 3886 1750 1487 558 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5489 276 Size: 53 83 186 936 3955 2468 Fragments arranged by size: 3955 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BsiWI BstXI BstZ17I DraIII EcoO109I FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI RsrII SanDI SexAI SfiI SrfI SwaI