(Circular) (Six-base) MAPSORT of: pFN21AA1998.seq Check: 1461 from: 1 to: 9820 pFN21AA1998 9820 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6363 6363 Size: 9820 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2658 5760 7630 278 Size: 53 83 186 936 1122 3102 1870 2468 Fragments arranged by size: 3102 2468 1870 1122 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3123 6834 7163 1937 Size: 1186 3711 329 4594 Fragments arranged by size: 4594 3711 1186 329 Acc65I G'GTAC_C Cuts at: 4286 7141 4286 Size: 2855 6965 AclI AA'CG_TT Cuts at: 1563 2163 7947 8320 1563 Size: 600 5784 373 3063 Fragments arranged by size: 5784 3063 600 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2106 2180 2928 3342 4288 4612 5598 5649 Size: 474 74 748 414 946 324 986 51 Cuts at: 5649 6146 6317 7883 9267 9535 1632 Size: 497 171 1566 1384 268 1917 Fragments arranged by size: 1917 1566 1384 986 946 748 497 474 414 324 268 171 74 51 AfeI AGC'GCT Cuts at: 5094 5094 Size: 9820 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9623 AflIII A'CryG_T Cuts at: 1203 2131 5221 8719 9696 1203 Size: 928 3090 3498 977 1327 Fragments arranged by size: 3498 3090 1327 977 928 AgeI A'CCGG_T Cuts at: 8651 8651 Size: 9820 AhdI GACnn_n'nnGTC Cuts at: 4559 6280 8549 4559 Size: 1721 2269 5830 Fragments arranged by size: 5830 2269 1721 AleI CACnn'nnGTG Cuts at: 1166 4042 1166 Size: 2876 6944 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5739 1184 Size: 4555 5265 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3520 3552 3520 Size: 32 9788 AlwNI CAG_nnn'CTG Cuts at: 2356 2906 3845 4606 5242 6541 6583 6893 7563 Size: 550 939 761 636 1299 42 310 670 Cuts at: 7563 9135 2356 Size: 1572 3041 Fragments arranged by size: 3041 1572 1299 939 761 670 636 550 310 42 ApaI G_GGCC'C Cuts at: 6574 6574 Size: 9820 ApaLI G'TGCA_C Cuts at: 4067 6323 7877 9033 4067 Size: 2256 1554 1156 4854 Fragments arranged by size: 4854 2256 1554 1156 ApoI r'AATT_y Cuts at: 1493 2662 2923 3693 3795 4109 4156 4680 4777 Size: 1169 261 770 102 314 47 524 97 Cuts at: 4777 4862 4930 4977 5505 5646 5688 6091 6136 Size: 85 68 47 528 141 42 403 45 Cuts at: 6136 6997 7129 7355 7507 9616 1493 Size: 861 132 226 152 2109 1697 Fragments arranged by size: 2109 1697 1169 861 770 528 524 403 314 261 226 152 141 132 102 97 85 68 47 47 45 42 AseI AT'TA_AT Cuts at: 160 1032 3177 8374 160 Size: 872 2145 5197 1606 Fragments arranged by size: 5197 2145 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9820 AvaI C'yCGr_G Cuts at: 1870 1943 2560 2694 6026 6647 7145 1870 Size: 73 617 134 3332 621 498 4545 Fragments arranged by size: 4545 3332 621 617 498 134 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4802 4835 4802 Size: 33 9787 BamHI G'GATC_C Cuts at: 4510 5550 6022 6030 7150 7517 4510 Size: 1040 472 8 1120 367 6813 Fragments arranged by size: 6813 1120 1040 472 367 8 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2600 4286 5885 7141 8596 Size: 325 217 627 813 1686 1599 1256 1455 Cuts at: 8596 618 Size: 1842 Fragments arranged by size: 1842 1686 1599 1455 1256 813 627 325 217 BanII G_rGCy'C Cuts at: 1391 2261 2412 3521 4397 5772 6574 7139 1391 Size: 870 151 1109 876 1375 802 565 4072 Fragments arranged by size: 4072 1375 1109 876 870 802 565 151 BbeI G_GCGC'C Cuts at: 5889 5889 Size: 9820 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2658 3012 5619 928 Size: 971 759 354 2607 5129 Fragments arranged by size: 5129 2607 971 759 354 BbvCI CC'TCA_GC Cuts at: 4460 6524 4460 Size: 2064 7756 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2309 2343 2582 2616 8011 8045 1903 Size: 34 372 34 239 34 5395 34 3678 Fragments arranged by size: 5395 3678 372 239 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7712 8922 7712 Size: 1210 8610 BclI T'GATC_A Cuts at: 1548 3145 5392 5469 1548 Size: 1597 2247 77 5899 Fragments arranged by size: 5899 2247 1597 77 BfrBI ATG'CAT Cuts at: 4591 4989 4591 Size: 398 9422 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2378 5639 6588 Size: 107 122 71 631 605 706 3261 949 Cuts at: 6588 8431 136 Size: 1843 1525 Fragments arranged by size: 3261 1843 1525 949 706 631 605 122 107 71 BglII A'GATC_T Cuts at: 4623 9815 4623 Size: 5192 4628 BlpI GC'TnA_GC Cuts at: 7237 7237 Size: 9820 Bme1580I G_kGCm'C Cuts at: 1790 4071 6327 6574 7881 9037 1790 Size: 2281 2256 247 1307 1156 2573 Fragments arranged by size: 2573 2281 2256 1307 1156 247 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9820 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2536 3270 3540 Size: 416 53 83 399 24 1119 734 270 Cuts at: 3540 7551 8509 442 Size: 4011 958 1753 Fragments arranged by size: 4011 1753 1119 958 734 416 399 270 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9820 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2720 2720 Size: 9820 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2153 2875 3489 3580 3675 4892 6200 6329 Size: 914 722 614 91 95 1217 1308 129 Cuts at: 6329 6473 6479 6536 7107 8480 1239 Size: 144 6 57 571 1373 2579 Fragments arranged by size: 2579 1373 1308 1217 914 722 614 571 144 129 95 91 57 6 Bpu10I CC'TnA_GC Cuts at: 4460 6524 4460 Size: 2064 7756 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3508 5189 6824 7945 8810 9108 3508 Size: 1681 1635 1121 865 298 4220 Fragments arranged by size: 4220 1681 1635 1121 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3516 4193 4368 8483 882 Size: 617 2017 677 175 4115 2219 Fragments arranged by size: 4115 2219 2017 677 617 175 BsaAI yAC'GTr Cuts at: 493 1204 2278 2627 7611 493 Size: 711 1074 349 4984 2702 Fragments arranged by size: 4984 2702 1074 711 349 BsaBI GATnn'nnATC Cuts at: 2048 3027 6021 9814 2048 Size: 979 2994 3793 2054 Fragments arranged by size: 3793 2994 2054 979 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2655 3453 4969 5757 Size: 53 83 186 936 1122 798 1516 788 Cuts at: 5757 5886 7627 8009 8644 275 Size: 129 1741 382 635 1451 Fragments arranged by size: 1741 1516 1451 1122 936 798 788 635 382 186 129 83 53 BsaWI w'CCGG_w Cuts at: 2245 2808 3580 8253 8651 8925 9072 9483 2245 Size: 563 772 4673 398 274 147 411 2582 Fragments arranged by size: 4673 2582 772 563 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5910 5940 6218 6248 6422 Size: 30 165 30 4507 30 278 30 174 Cuts at: 6422 6428 6452 6458 1178 Size: 6 24 6 4540 Fragments arranged by size: 4540 4507 278 174 165 30 30 30 30 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2420 3511 4661 5465 5720 6323 6467 6628 Size: 1234 1091 1150 804 255 603 144 161 Cuts at: 6628 1186 Size: 4378 Fragments arranged by size: 4378 1234 1150 1091 804 603 255 161 144 BseYI C'CCAG_C Cuts at: 3327 6573 6586 9023 9519 3327 Size: 3246 13 2437 496 3628 Fragments arranged by size: 3628 3246 2437 496 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2720 2919 1163 Size: 573 984 199 8064 Fragments arranged by size: 8064 984 573 199 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4792 6607 7270 8031 9059 1540 Size: 275 186 2791 1815 663 761 1028 2301 Fragments arranged by size: 2791 2301 1815 1028 761 663 275 186 BsiHKAI G_wGCw'C Cuts at: 2389 2412 3521 4071 6327 6633 7881 7966 9037 Size: 23 1109 550 2256 306 1248 85 1071 Cuts at: 9037 2389 Size: 3172 Fragments arranged by size: 3172 2256 1248 1109 1071 550 306 85 23 BsmI GAATG_Cn' Cuts at: 3475 4989 4991 7331 7424 3475 Size: 1514 2 2340 93 5871 Fragments arranged by size: 5871 2340 1514 93 2 BsmBI CGTCTCn'nnnn_ Cuts at: 2195 5539 6064 6152 2195 Size: 3344 525 88 5863 Fragments arranged by size: 5863 3344 525 88 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2261 2389 2412 3521 4071 4397 5772 Size: 399 471 128 23 1109 550 326 1375 Cuts at: 5772 6327 6574 6633 7139 7881 7966 9037 1391 Size: 555 247 59 506 742 85 1071 2174 Fragments arranged by size: 2174 1375 1109 1071 742 555 550 506 471 399 326 247 128 85 59 23 BspEI T'CCGG_A Cuts at: 2808 9483 2808 Size: 6675 3145 BspHI T'CATG_A Cuts at: 2136 3882 5950 6685 7709 9439 2136 Size: 1746 2068 735 1024 1730 2517 Fragments arranged by size: 2517 2068 1746 1730 1024 735 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3838 4350 6363 7175 844 Size: 2994 512 2013 812 3489 Fragments arranged by size: 3489 2994 2013 812 512 BsrBI CCG'CTC Cuts at: 1394 1823 5745 6427 6433 6520 7707 1394 Size: 429 3922 682 6 87 1187 3507 Fragments arranged by size: 3922 3507 1187 682 429 87 6 BsrDI GCAATG_nn' Cuts at: 65 1248 3071 5660 8315 8489 65 Size: 1183 1823 2589 2655 174 1396 Fragments arranged by size: 2655 2589 1823 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3087 6606 8464 8651 1700 Size: 110 1277 3519 1858 187 2869 Fragments arranged by size: 3519 2869 1858 1277 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9820 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9820 BssSI C'ACGA_G Cuts at: 2411 7880 8892 2411 Size: 5469 1012 3339 Fragments arranged by size: 5469 3339 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2393 6583 794 Size: 1599 4190 4031 Fragments arranged by size: 4190 4031 1599 BstBI TT'CG_AA Cuts at: 6863 8624 6863 Size: 1761 8059 BstEII G'GTnAC_C Cuts at: 1184 2248 6043 1184 Size: 1064 3795 4961 Fragments arranged by size: 4961 3795 1064 BstXI CCAn_nnnn'nTGG Cuts at: 2647 4324 2647 Size: 1677 8143 BstYI r'GATC_y Cuts at: 1972 2117 4510 4623 5172 5550 6022 6030 6198 Size: 145 2393 113 549 378 472 8 168 Cuts at: 6198 7150 7517 7902 7919 9371 9457 9469 9815 Size: 952 367 385 17 1452 86 12 346 Cuts at: 9815 1972 Size: 1977 Fragments arranged by size: 2393 1977 1452 952 549 472 385 378 367 346 168 145 113 86 17 12 8 BtgI C'CryG_G Cuts at: 513 1065 2004 2188 2253 4389 4671 6624 513 Size: 552 939 184 65 2136 282 1953 3709 Fragments arranged by size: 3709 2136 1953 939 552 282 184 65 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9081 Fragments arranged by size: 9081 683 56 BtsI GCAGTG_nn' Cuts at: 2387 3192 3644 4004 4037 4049 4471 5871 7336 Size: 805 452 360 33 12 422 1400 1465 Cuts at: 7336 8130 8150 2387 Size: 794 20 4057 Fragments arranged by size: 4057 1465 1400 805 794 452 422 360 33 20 12 ClaI AT'CG_AT Cuts at: 1554 6667 1554 Size: 5113 4707 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9785 DraI TTT'AAA Cuts at: 4900 5133 7124 4900 Size: 233 1991 7596 Fragments arranged by size: 7596 1991 233 DraIII CAC_nnn'GTG Cuts at: 3231 3293 3231 Size: 62 9758 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6056 8827 809 Size: 507 4740 2771 1802 Fragments arranged by size: 4740 2771 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5640 6054 6241 Size: 54 1425 50 275 18 3810 414 187 Cuts at: 6241 6315 6604 6901 7267 8156 9785 8 Size: 74 289 297 366 889 1629 43 Fragments arranged by size: 3810 1629 1425 889 414 366 297 289 275 187 74 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6604 7267 1537 Size: 275 4792 663 4090 Fragments arranged by size: 4792 4090 663 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2505 2589 2810 2907 3744 4402 5998 7750 Size: 1152 84 221 97 837 658 1596 1752 Cuts at: 7750 1353 Size: 3423 Fragments arranged by size: 3423 1752 1596 1152 837 658 221 97 84 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5948 8407 8781 8927 387 Size: 1064 138 4359 2459 374 146 1280 Fragments arranged by size: 4359 2459 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2410 3519 2410 Size: 1109 8711 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2106 2153 2180 2875 2928 3342 3489 Size: 393 474 47 27 695 53 414 147 Cuts at: 3489 3580 3675 4288 4612 4892 5598 5649 6146 Size: 91 95 613 324 280 706 51 497 Cuts at: 6146 6200 6317 6329 6473 6479 6536 7107 7883 Size: 54 117 12 144 6 57 571 776 Cuts at: 7883 8480 9267 9535 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 776 706 695 613 597 571 497 474 414 393 324 280 268 147 144 117 95 91 57 54 53 51 47 27 12 6 EcoNI CCTnn'n_nnAGG Cuts at: 5793 5793 Size: 9820 EcoO109I rG'GnC_Cy Cuts at: 2524 6229 2524 Size: 3705 6115 EcoRI G'AATT_C Cuts at: 2923 3795 4862 4977 7129 7507 2923 Size: 872 1067 115 2152 378 5236 Fragments arranged by size: 5236 2152 1067 872 378 115 EcoRV GAT'ATC Cuts at: 3043 3166 5317 3043 Size: 123 2151 7546 Fragments arranged by size: 7546 2151 123 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2048 2048 Size: 9820 FspI TGC'GCA Cuts at: 6371 8326 6371 Size: 1955 7865 HaeII r_GCGC'y Cuts at: 1678 5096 5889 6364 6541 8967 1678 Size: 3418 793 475 177 2426 2531 Fragments arranged by size: 3418 2531 2426 793 475 177 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3886 3918 4545 4577 5910 Size: 32 135 32 2314 32 627 32 1333 Cuts at: 5910 5942 6218 6250 6266 6298 6428 6460 6667 Size: 32 276 32 16 32 130 32 207 Cuts at: 6667 6699 8461 8493 8535 8567 1373 Size: 32 1762 32 42 32 2626 Fragments arranged by size: 2626 2314 1762 1333 627 276 207 135 130 42 32 32 32 32 32 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 669 1657 1938 4891 7164 7410 669 Size: 988 281 2953 2273 246 3079 Fragments arranged by size: 3079 2953 2273 988 281 246 HindIII A'AGCT_T Cuts at: 748 2686 3759 4300 5423 6711 748 Size: 1938 1073 541 1123 1288 3857 Fragments arranged by size: 3857 1938 1288 1123 1073 541 HpaI GTT'AAC Cuts at: 4891 7410 4891 Size: 2519 7301 KasI G'GCGC_C Cuts at: 5885 5885 Size: 9820 KpnI G_GTAC'C Cuts at: 4290 7145 4290 Size: 2855 6965 MfeI C'AATT_G Cuts at: 7419 7419 Size: 9820 MluI A'CGCG_T Cuts at: 9696 9696 Size: 9820 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2766 3390 4201 8934 9118 1305 Size: 1461 624 811 4733 184 2007 Fragments arranged by size: 4733 2007 1461 811 624 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5642 6317 10 Size: 54 1425 343 3810 675 3513 Fragments arranged by size: 3810 3513 1425 675 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4042 4371 5721 6771 7778 8137 8296 Size: 648 2876 329 1350 1050 1007 359 159 Cuts at: 8296 518 Size: 2042 Fragments arranged by size: 2876 2042 1350 1050 1007 648 359 329 159 MspA1I CmG'CkG Cuts at: 1606 2895 4350 4657 6358 6391 6626 6893 7236 Size: 1289 1455 307 1701 33 235 267 343 Cuts at: 7236 7913 9061 9306 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1701 1455 1289 1148 677 343 307 267 245 235 33 NaeI GCC'GGC Cuts at: 3089 6608 3089 Size: 3519 6301 NarI GG'CG_CC Cuts at: 5886 5886 Size: 9820 NcoI C'CATG_G Cuts at: 513 1065 2004 2188 2253 4389 513 Size: 552 939 184 65 2136 5944 Fragments arranged by size: 5944 2136 939 552 184 65 NdeI CA'TA_TG Cuts at: 387 387 Size: 9820 NgoMIV G'CCGG_C Cuts at: 3087 6606 3087 Size: 3519 6301 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9820 NotI GC'GGCC_GC Cuts at: 7267 7267 Size: 9820 NruI TCG'CGA Cuts at: 6288 6288 Size: 9820 NsiI A_TGCA'T Cuts at: 4593 4991 4593 Size: 398 9422 NspI r_CATG'y Cuts at: 1132 1229 2135 3103 4101 5225 7178 8723 1132 Size: 97 906 968 998 1124 1953 1545 2229 Fragments arranged by size: 2229 1953 1545 1124 998 968 906 97 PacI TTA_AT'TAA Cuts at: 5106 5106 Size: 9820 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9820 PciI A'CATG_T Cuts at: 2131 5221 8719 2131 Size: 3090 3498 3232 Fragments arranged by size: 3498 3232 3090 PflMI CCAn_nnn'nTGG Cuts at: 1108 2189 2578 4184 6040 6208 6541 1108 Size: 1081 389 1606 1856 168 333 4387 Fragments arranged by size: 4387 1856 1606 1081 389 333 168 PfoI T'CCnGG_A Cuts at: 6495 6495 Size: 9820 PmeI GTTT'AAAC Cuts at: 7124 7124 Size: 9820 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7889 7921 9402 9434 7889 Size: 32 1481 32 8275 Fragments arranged by size: 8275 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9820 PsiI TTA'TAA Cuts at: 7028 7390 7028 Size: 362 9458 PspOMI G'GGCC_C Cuts at: 6570 6570 Size: 9820 PstI C_TGCA'G Cuts at: 830 2021 2345 3085 3999 4044 4355 4656 6423 Size: 1191 324 740 914 45 311 301 1767 Cuts at: 6423 7020 7172 830 Size: 597 152 3478 Fragments arranged by size: 3478 1767 1191 914 740 597 324 311 301 152 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9820 PvuII CAG'CTG Cuts at: 4350 4657 4350 Size: 307 9513 SacI G_AGCT'C Cuts at: 2412 3521 2412 Size: 1109 8711 SacII CC_GC'GG Cuts at: 6627 6627 Size: 9820 SalI G'TCGA_C Cuts at: 1936 7162 1936 Size: 5226 4594 SapI GCTCTTCn'nnn_ Cuts at: 3744 4402 3744 Size: 658 9162 SbfI CC_TGCA'GG Cuts at: 7172 7172 Size: 9820 ScaI AGT'ACT Cuts at: 8068 9780 8068 Size: 1712 8108 SfcI C'TryA_G Cuts at: 826 1045 2017 2341 3081 3261 3629 3995 4040 Size: 219 972 324 740 180 368 366 45 Cuts at: 4040 4351 4382 4407 4652 4729 5298 5562 6419 Size: 311 31 25 245 77 569 264 857 Cuts at: 6419 7016 7168 8303 8984 9175 826 Size: 597 152 1135 681 191 1471 Fragments arranged by size: 1471 1135 972 857 740 681 597 569 368 366 324 311 264 245 219 191 180 152 77 45 31 25 SfoI GGC'GCC Cuts at: 5887 5887 Size: 9820 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9820 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9820 SmaI CCC'GGG Cuts at: 1872 2562 6028 7147 1872 Size: 690 3466 1119 4545 Fragments arranged by size: 4545 3466 1119 690 SmlI C'TyrA_G Cuts at: 820 1017 1943 3523 5168 6839 7924 8825 9087 Size: 197 926 1580 1645 1671 1085 901 262 Cuts at: 9087 820 Size: 1553 Fragments arranged by size: 1671 1645 1580 1553 1085 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 7611 493 Size: 7118 2702 SpeI A'CTAG_T Cuts at: 152 6818 152 Size: 6666 3154 SphI G_CATG'C Cuts at: 1132 4101 7178 1132 Size: 2969 3077 3774 Fragments arranged by size: 3774 3077 2969 SspI AAT'ATT Cuts at: 5 52 7108 7744 5 Size: 47 7056 636 2081 Fragments arranged by size: 7056 2081 636 47 StuI AGG'CCT Cuts at: 1517 2576 2846 5873 1517 Size: 1059 270 3027 5464 Fragments arranged by size: 5464 3027 1059 270 StyI C'CwwG_G Cuts at: 513 1065 2004 2188 2253 2397 2577 3447 4150 Size: 552 939 184 65 144 180 870 703 Cuts at: 4150 4293 4323 4389 5520 7259 513 Size: 143 30 66 1131 1739 3074 Fragments arranged by size: 3074 1739 1131 939 870 703 552 184 180 144 143 66 65 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3703 4806 7847 7864 8017 8202 8647 Size: 1255 2123 1103 3041 17 153 185 445 Cuts at: 8647 325 Size: 1498 Fragments arranged by size: 3041 2123 1498 1255 1103 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5049 6718 8066 Size: 274 80 33 51 1443 3072 1669 1348 Cuts at: 8066 9778 96 Size: 1712 138 Fragments arranged by size: 3072 1712 1669 1443 1348 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3526 3558 4131 4163 4190 4222 5501 5533 6022 Size: 32 573 32 27 32 1279 32 489 Cuts at: 6022 6054 6422 6454 6959 6991 9780 9812 3526 Size: 32 368 32 505 32 2789 32 3534 Fragments arranged by size: 3534 2789 1279 573 505 489 368 32 32 32 32 32 32 32 32 27 Tth111I GACn'n_nGTC Cuts at: 1316 5924 1316 Size: 4608 5212 XbaI T'CTAG_A Cuts at: 2443 5764 7156 2443 Size: 3321 1392 5107 Fragments arranged by size: 5107 3321 1392 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9820 XmaI C'CCGG_G Cuts at: 1870 2560 6026 7145 1870 Size: 690 3466 1119 4545 Fragments arranged by size: 4545 3466 1119 690 XmnI GAAnn'nnTTC Cuts at: 2467 2499 3609 4159 4989 7949 2467 Size: 32 1110 550 830 2960 4338 Fragments arranged by size: 4338 2960 1110 830 550 32 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2656 5758 7628 276 Size: 53 83 186 936 1122 3102 1870 2468 Fragments arranged by size: 3102 2468 1870 1122 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BsiWI BstZ17I Bsu36I FseI FspAI PmlI PpuMI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI XcmI