(Circular) (Six-base) MAPSORT of: pFN21AA1983.seq Check: 748 from: 1 to: 5923 pFN21AA1983 5923 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3733 278 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3266 1937 Size: 1329 4594 Acc65I G'GTAC_C Cuts at: 3244 3244 Size: 5923 AclI AA'CG_TT Cuts at: 1563 4050 4423 1563 Size: 2487 373 3063 Fragments arranged by size: 3063 2487 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2175 2204 2669 3078 3986 5370 5638 1632 Size: 543 29 465 409 908 1384 268 1917 Fragments arranged by size: 1917 1384 908 543 465 409 268 29 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5726 AflIII A'CryG_T Cuts at: 1203 2097 2522 4822 5799 1203 Size: 894 425 2300 977 1327 Fragments arranged by size: 2300 1327 977 894 425 AgeI A'CCGG_T Cuts at: 4754 4754 Size: 5923 AhdI GACnn_n'nnGTC Cuts at: 4652 4652 Size: 5923 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5923 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2676 1184 Size: 1492 4431 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3056 3088 3056 Size: 32 5891 AlwNI CAG_nnn'CTG Cuts at: 3666 5238 3666 Size: 1572 4351 ApaI G_GGCC'C Cuts at: 2774 2915 2979 2774 Size: 141 64 5718 Fragments arranged by size: 5718 141 64 ApaLI G'TGCA_C Cuts at: 2308 3980 5136 2308 Size: 1672 1156 3095 Fragments arranged by size: 3095 1672 1156 ApoI r'AATT_y Cuts at: 1493 2240 3122 3232 3458 3610 5719 1493 Size: 747 882 110 226 152 2109 1697 Fragments arranged by size: 2109 1697 882 747 226 152 110 AseI AT'TA_AT Cuts at: 160 1032 4477 160 Size: 872 3445 1606 Fragments arranged by size: 3445 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 AvaI C'yCGr_G Cuts at: 1870 1943 3248 1870 Size: 73 1305 4545 Fragments arranged by size: 4545 1305 73 BamHI G'GATC_C Cuts at: 3253 3620 3253 Size: 367 5556 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2932 3078 3244 4699 Size: 325 217 627 221 924 146 166 1455 Cuts at: 4699 618 Size: 1842 Fragments arranged by size: 1842 1455 924 627 325 221 217 166 146 BanII G_rGCy'C Cuts at: 1391 2202 2774 2855 2915 2979 3158 3205 3242 Size: 811 572 81 60 64 179 47 37 Cuts at: 3242 1391 Size: 4072 Fragments arranged by size: 4072 811 572 179 81 64 60 47 37 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2181 2525 928 Size: 971 282 344 4326 Fragments arranged by size: 4326 971 344 282 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2264 2298 4114 4148 1903 Size: 34 327 34 1816 34 3678 Fragments arranged by size: 3678 1816 327 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2345 3815 5025 2345 Size: 1470 1210 3243 Fragments arranged by size: 3243 1470 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5923 BfrBI ATG'CAT Cuts at: 2259 2259 Size: 5923 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2052 2493 2778 Size: 107 122 71 631 605 380 441 285 Cuts at: 2778 2850 2910 4534 136 Size: 72 60 1624 1525 Fragments arranged by size: 1624 1525 631 605 441 380 285 122 107 72 71 60 BglII A'GATC_T Cuts at: 5918 5918 Size: 5923 BlpI GC'TnA_GC Cuts at: 3340 3340 Size: 5923 Bme1580I G_kGCm'C Cuts at: 1790 2312 2446 2774 2915 2935 2979 3081 3984 Size: 522 134 328 141 20 44 102 903 Cuts at: 3984 5140 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 903 522 328 141 134 102 44 20 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5923 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3215 3654 4612 Size: 416 53 83 399 24 1798 439 958 Cuts at: 4612 442 Size: 1753 Fragments arranged by size: 1798 1753 958 439 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5923 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2973 3180 4583 1239 Size: 1734 207 1403 2579 Fragments arranged by size: 2579 1734 1403 207 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3217 4048 4913 5211 3217 Size: 831 865 298 3929 Fragments arranged by size: 3929 865 831 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2991 4586 882 Size: 617 1492 1595 2219 Fragments arranged by size: 2219 1595 1492 617 BsaAI yAC'GTr Cuts at: 493 1204 2100 3714 493 Size: 711 896 1614 2702 Fragments arranged by size: 2702 1614 896 711 BsaBI GATnn'nnATC Cuts at: 3168 5917 3168 Size: 2749 3174 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3730 4112 4747 275 Size: 53 83 186 936 2197 382 635 1451 Fragments arranged by size: 2197 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2376 3081 4356 4754 5028 5175 5586 2376 Size: 705 1275 398 274 147 411 2713 Fragments arranged by size: 2713 1275 705 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2050 2080 1178 Size: 30 165 30 647 30 5021 Fragments arranged by size: 5021 647 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2046 2129 2979 3116 1186 Size: 860 83 850 137 3993 Fragments arranged by size: 3993 860 850 137 83 BseYI C'CCAG_C Cuts at: 2050 3128 5126 5622 2050 Size: 1078 1998 496 2351 Fragments arranged by size: 2351 1998 1078 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5350 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3373 4134 5162 1540 Size: 275 186 1372 761 1028 2301 Fragments arranged by size: 2301 1372 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2202 2312 3984 4069 5140 2202 Size: 110 1672 85 1071 2985 Fragments arranged by size: 2985 1672 1071 110 85 BsmI GAATG_Cn' Cuts at: 3434 3527 3434 Size: 93 5830 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2202 2312 2446 2774 2855 2915 2935 Size: 399 412 110 134 328 81 60 20 Cuts at: 2935 2979 3081 3158 3205 3242 3984 4069 5140 Size: 44 102 77 47 37 742 85 1071 Cuts at: 5140 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 412 399 328 134 110 102 85 81 77 60 47 44 37 20 BspEI T'CCGG_A Cuts at: 5586 5586 Size: 5923 BspHI T'CATG_A Cuts at: 3812 5542 3812 Size: 1730 4193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3278 844 Size: 2434 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3810 1394 Size: 429 1987 3507 Fragments arranged by size: 3507 1987 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2434 3049 4418 4592 65 Size: 1183 1186 615 1369 174 1396 Fragments arranged by size: 1396 1369 1186 1183 615 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4567 4754 1700 Size: 110 2757 187 2869 Fragments arranged by size: 2869 2757 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5923 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5923 BssSI C'ACGA_G Cuts at: 3983 4995 3983 Size: 1012 4911 BstAPI GCAn_nnn'nTGC Cuts at: 794 2638 794 Size: 1844 4079 BstBI TT'CG_AA Cuts at: 4727 4727 Size: 5923 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5923 BstXI CCAn_nnnn'nTGG Cuts at: 3151 3151 Size: 5923 BstYI r'GATC_y Cuts at: 1972 3253 3620 4005 4022 5474 5560 5572 5918 Size: 1281 367 385 17 1452 86 12 346 Cuts at: 5918 1972 Size: 1977 Fragments arranged by size: 1977 1452 1281 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3117 3117 Size: 5923 BtgI C'CryG_G Cuts at: 513 1065 2004 2846 2865 3144 513 Size: 552 939 842 19 279 3292 Fragments arranged by size: 3292 939 842 552 279 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5184 Fragments arranged by size: 5184 683 56 BtsI GCAGTG_nn' Cuts at: 2312 3439 4233 4253 2312 Size: 1127 794 20 3982 Fragments arranged by size: 3982 1127 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5923 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5888 DraI TTT'AAA Cuts at: 3227 3227 Size: 5923 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4930 809 Size: 507 3614 1802 Fragments arranged by size: 3614 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2323 2557 3370 Size: 54 1425 50 275 18 493 234 813 Cuts at: 3370 4259 5888 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 813 493 275 234 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3370 1537 Size: 275 1558 4090 Fragments arranged by size: 4090 1558 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2935 3097 3853 1353 Size: 1582 162 756 3423 Fragments arranged by size: 3423 1582 756 162 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4510 4884 5030 387 Size: 1064 138 2921 374 146 1280 Fragments arranged by size: 2921 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2200 2200 Size: 5923 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2175 2204 2669 2973 3078 3180 3986 Size: 393 543 29 465 304 105 102 806 Cuts at: 3986 4583 5370 5638 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 806 787 597 543 465 393 304 268 105 102 29 EcoO109I rG'GnC_Cy Cuts at: 2744 2770 2771 2825 2861 2911 2912 2993 2744 Size: 26 1 54 36 50 1 81 5674 Fragments arranged by size: 5674 81 54 50 36 26 1 1 EcoRI G'AATT_C Cuts at: 3232 3610 3232 Size: 378 5545 FspI TGC'GCA Cuts at: 4429 4429 Size: 5923 HaeII r_GCGC'y Cuts at: 1678 2972 5070 1678 Size: 1294 2098 2531 Fragments arranged by size: 2531 2098 1294 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2050 2082 2244 2276 3140 Size: 32 135 32 478 32 162 32 864 Cuts at: 3140 3172 4564 4596 4638 4670 1373 Size: 32 1392 32 42 32 2626 Fragments arranged by size: 2626 1392 864 478 162 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3267 3513 669 Size: 988 281 1329 246 3079 Fragments arranged by size: 3079 1329 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5923 HpaI GTT'AAC Cuts at: 3513 3513 Size: 5923 KpnI G_GTAC'C Cuts at: 3248 3248 Size: 5923 MfeI C'AATT_G Cuts at: 3522 3522 Size: 5923 MluI A'CGCG_T Cuts at: 5799 5799 Size: 5923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5037 5221 1305 Size: 3732 184 2007 Fragments arranged by size: 3732 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2559 10 Size: 54 1425 343 727 3374 Fragments arranged by size: 3374 1425 727 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3881 4240 4399 518 Size: 648 2715 359 159 2042 Fragments arranged by size: 2715 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2050 2071 2478 2647 2684 3339 4016 5164 Size: 444 21 407 169 37 655 677 1148 Cuts at: 5164 5409 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 655 444 407 245 169 37 21 NcoI C'CATG_G Cuts at: 513 1065 2004 2865 3144 513 Size: 552 939 861 279 3292 Fragments arranged by size: 3292 939 861 552 279 NdeI CA'TA_TG Cuts at: 387 387 Size: 5923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5923 NotI GC'GGCC_GC Cuts at: 3370 3370 Size: 5923 NruI TCG'CGA Cuts at: 2162 2162 Size: 5923 NsiI A_TGCA'T Cuts at: 2261 2261 Size: 5923 NspI r_CATG'y Cuts at: 1132 1229 2211 2526 2610 3281 4826 1132 Size: 97 982 315 84 671 1545 2229 Fragments arranged by size: 2229 1545 982 671 315 97 84 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5923 PciI A'CATG_T Cuts at: 2522 4822 2522 Size: 2300 3623 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5923 PfoI T'CCnGG_A Cuts at: 2347 2502 2740 2989 2347 Size: 155 238 249 5281 Fragments arranged by size: 5281 249 238 155 PmeI GTTT'AAAC Cuts at: 3227 3227 Size: 5923 PmlI CAC'GTG Cuts at: 2100 2100 Size: 5923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3992 4024 5505 5537 3992 Size: 32 1481 32 4378 Fragments arranged by size: 4378 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2744 2861 2993 2744 Size: 117 132 5674 Fragments arranged by size: 5674 132 117 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5923 PsiI TTA'TAA Cuts at: 3493 3493 Size: 5923 PspOMI G'GGCC_C Cuts at: 2770 2911 2975 2770 Size: 141 64 5718 Fragments arranged by size: 5718 141 64 PstI C_TGCA'G Cuts at: 830 2646 3063 3275 830 Size: 1816 417 212 3478 Fragments arranged by size: 3478 1816 417 212 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5923 PvuII CAG'CTG Cuts at: 2050 2647 2684 2050 Size: 597 37 5289 Fragments arranged by size: 5289 597 37 SacI G_AGCT'C Cuts at: 2202 2202 Size: 5923 SalI G'TCGA_C Cuts at: 1936 3265 1936 Size: 1329 4594 SanDI GG'GwC_CC Cuts at: 2861 2861 Size: 5923 SbfI CC_TGCA'GG Cuts at: 3275 3275 Size: 5923 ScaI AGT'ACT Cuts at: 4171 5883 4171 Size: 1712 4211 SfcI C'TryA_G Cuts at: 826 1045 2107 2212 2642 3059 3271 4406 5087 Size: 219 1062 105 430 417 212 1135 681 Cuts at: 5087 5278 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1062 681 430 417 219 212 191 105 SfiI GGCCn_nnn'nGGCC Cuts at: 2910 2910 Size: 5923 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5923 SmaI CCC'GGG Cuts at: 1872 3250 1872 Size: 1378 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3196 4027 4928 5190 820 Size: 197 926 1253 831 901 262 1553 Fragments arranged by size: 1553 1253 926 901 831 262 197 SnaBI TAC'GTA Cuts at: 493 3714 493 Size: 3221 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5923 SphI G_CATG'C Cuts at: 1132 3281 1132 Size: 2149 3774 SspI AAT'ATT Cuts at: 5 52 3847 5 Size: 47 3795 2081 Fragments arranged by size: 3795 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5923 StyI C'CwwG_G Cuts at: 513 1065 2004 2463 2865 3144 3362 513 Size: 552 939 459 402 279 218 3074 Fragments arranged by size: 3074 939 552 459 402 279 218 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3950 3967 4120 4305 4750 325 Size: 1255 2370 17 153 185 445 1498 Fragments arranged by size: 2370 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2336 4169 5881 Size: 274 80 33 51 1443 359 1833 1712 Cuts at: 5881 96 Size: 138 Fragments arranged by size: 1833 1712 1443 359 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2425 2457 2722 2754 5883 5915 2425 Size: 32 265 32 3129 32 2433 Fragments arranged by size: 3129 2433 265 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5923 XbaI T'CTAG_A Cuts at: 3259 3259 Size: 5923 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2047 2047 Size: 5923 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5923 XmaI C'CCGG_G Cuts at: 1870 3248 1870 Size: 1378 4545 XmnI GAAnn'nnTTC Cuts at: 4052 4052 Size: 5923 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3731 276 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV PacI PspXI PsrI RsrII SacII SapI SexAI SfoI SrfI SwaI