(Circular) (Six-base) MAPSORT of: pFN21AA1975.seq Check: 5086 from: 1 to: 6355 pFN21AA1975 6355 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3375 3375 Size: 6355 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3546 4165 278 Size: 53 83 186 936 2010 619 2468 Fragments arranged by size: 2468 2010 936 619 186 83 53 AccI GT'mk_AC Cuts at: 1937 3698 1937 Size: 1761 4594 Acc65I G'GTAC_C Cuts at: 3676 3676 Size: 6355 AclI AA'CG_TT Cuts at: 1563 4482 4855 1563 Size: 2919 373 3063 Fragments arranged by size: 3063 2919 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2432 2662 4418 5802 6070 1632 Size: 800 230 1756 1384 268 1917 Fragments arranged by size: 1917 1756 1384 800 268 230 AfeI AGC'GCT Cuts at: 3576 3576 Size: 6355 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6158 AflIII A'CryG_T Cuts at: 1203 5254 6231 1203 Size: 4051 977 1327 Fragments arranged by size: 4051 1327 977 AgeI A'CCGG_T Cuts at: 5186 5186 Size: 6355 AhdI GACnn_n'nnGTC Cuts at: 5084 5084 Size: 6355 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6355 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3635 1184 Size: 2451 3904 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3279 3311 3279 Size: 32 6323 AlwNI CAG_nnn'CTG Cuts at: 2923 3225 3367 3523 4098 5670 2923 Size: 302 142 156 575 1572 3608 Fragments arranged by size: 3608 1572 575 302 156 142 ApaLI G'TGCA_C Cuts at: 2117 4412 5568 2117 Size: 2295 1156 2904 Fragments arranged by size: 2904 2295 1156 ApoI r'AATT_y Cuts at: 1493 3664 3890 4042 6151 1493 Size: 2171 226 152 2109 1697 Fragments arranged by size: 2171 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4909 160 Size: 872 3877 1606 Fragments arranged by size: 3877 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6355 AvaI C'yCGr_G Cuts at: 1870 1943 3552 3680 1870 Size: 73 1609 128 4545 Fragments arranged by size: 4545 1609 128 73 BamHI G'GATC_C Cuts at: 3294 3685 4052 3294 Size: 391 367 5597 Fragments arranged by size: 5597 391 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3125 3676 5131 618 Size: 325 217 627 1338 551 1455 1842 Fragments arranged by size: 1842 1455 1338 627 551 325 217 BanII G_rGCy'C Cuts at: 1391 3180 3674 1391 Size: 1789 494 4072 Fragments arranged by size: 4072 1789 494 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2420 928 Size: 971 521 4863 Fragments arranged by size: 4863 971 521 BbvCI CC'TCA_GC Cuts at: 2808 2808 Size: 6355 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4546 4580 1903 Size: 34 2609 34 3678 Fragments arranged by size: 3678 2609 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4247 5457 4247 Size: 1210 5145 BclI T'GATC_A Cuts at: 1548 1548 Size: 6355 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2570 3593 4966 Size: 107 122 71 631 605 898 1023 1373 Cuts at: 4966 136 Size: 1525 Fragments arranged by size: 1525 1373 1023 898 631 605 122 107 71 BglII A'GATC_T Cuts at: 2066 6350 2066 Size: 4284 2071 BlpI GC'TnA_GC Cuts at: 3772 3772 Size: 6355 Bme1580I G_kGCm'C Cuts at: 1790 2121 4416 5572 1790 Size: 331 2295 1156 2573 Fragments arranged by size: 2573 2295 1156 331 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6355 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2679 4086 5044 Size: 416 53 83 399 24 1262 1407 958 Cuts at: 5044 442 Size: 1753 Fragments arranged by size: 1753 1407 1262 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6355 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2348 2572 2677 3173 3583 5015 1239 Size: 1109 224 105 496 410 1432 2579 Fragments arranged by size: 2579 1432 1109 496 410 224 105 Bpu10I CC'TnA_GC Cuts at: 2476 2808 3179 2476 Size: 332 371 5652 Fragments arranged by size: 5652 371 332 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4480 5345 5643 4480 Size: 865 298 5192 Fragments arranged by size: 5192 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3038 3440 5018 882 Size: 617 1539 402 1578 2219 Fragments arranged by size: 2219 1578 1539 617 402 BsaAI yAC'GTr Cuts at: 493 1204 4146 493 Size: 711 2942 2702 Fragments arranged by size: 2942 2702 711 BsaBI GATnn'nnATC Cuts at: 6349 6349 Size: 6355 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3543 4162 4544 5179 Size: 53 83 186 936 2010 619 382 635 Cuts at: 5179 275 Size: 1451 Fragments arranged by size: 2010 1451 936 635 619 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4788 5186 5460 5607 6018 4788 Size: 398 274 147 411 5125 Fragments arranged by size: 5125 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2150 2180 1178 Size: 30 165 30 747 30 5353 Fragments arranged by size: 5353 747 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2158 2897 3449 1186 Size: 972 739 552 4092 Fragments arranged by size: 4092 972 739 552 BseYI C'CCAG_C Cuts at: 2186 2659 2964 5558 6054 2186 Size: 473 305 2594 496 2487 Fragments arranged by size: 2594 2487 496 473 305 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3325 3454 1163 Size: 573 1589 129 4064 Fragments arranged by size: 4064 1589 573 129 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3805 4566 5594 1540 Size: 275 186 1804 761 1028 2301 Fragments arranged by size: 2301 1804 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2121 2460 3180 4416 4501 5572 2121 Size: 339 720 1236 85 1071 2904 Fragments arranged by size: 2904 1236 1071 720 339 85 BsmI GAATG_Cn' Cuts at: 3866 3959 3866 Size: 93 6262 BsmBI CGTCTCn'nnnn_ Cuts at: 3456 3456 Size: 6355 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2121 2460 3180 3674 4416 4501 5572 Size: 399 331 339 720 494 742 85 1071 Cuts at: 5572 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 720 494 399 339 331 85 BspEI T'CCGG_A Cuts at: 6018 6018 Size: 6355 BspHI T'CATG_A Cuts at: 4244 5974 4244 Size: 1730 4625 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2727 3375 3402 3710 844 Size: 1883 648 27 308 3489 Fragments arranged by size: 3489 1883 648 308 27 BsrBI CCG'CTC Cuts at: 1394 1823 2355 2887 4242 1394 Size: 429 532 532 1355 3507 Fragments arranged by size: 3507 1355 532 532 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2608 3211 4850 5024 65 Size: 1183 1360 603 1639 174 1396 Fragments arranged by size: 1639 1396 1360 1183 603 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4999 5186 1700 Size: 110 3189 187 2869 Fragments arranged by size: 3189 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6355 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6355 BssSI C'ACGA_G Cuts at: 4415 5427 4415 Size: 1012 5343 BstAPI GCAn_nnn'nTGC Cuts at: 794 3370 794 Size: 2576 3779 BstBI TT'CG_AA Cuts at: 5159 5159 Size: 6355 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6355 BstXI CCAn_nnnn'nTGG Cuts at: 2550 2620 2550 Size: 70 6285 BstYI r'GATC_y Cuts at: 1972 2066 2919 3294 3685 4052 4437 4454 5906 Size: 94 853 375 391 367 385 17 1452 Cuts at: 5906 5992 6004 6350 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 853 391 385 375 367 346 94 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2262 2988 3562 513 Size: 552 1197 726 574 3306 Fragments arranged by size: 3306 1197 726 574 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5616 Fragments arranged by size: 5616 683 56 BtsI GCAGTG_nn' Cuts at: 2226 2802 3028 3871 4665 4685 2226 Size: 576 226 843 794 20 3896 Fragments arranged by size: 3896 843 794 576 226 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6355 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6320 DraI TTT'AAA Cuts at: 2154 2866 3659 2154 Size: 712 793 4850 Fragments arranged by size: 4850 793 712 DraIII CAC_nnn'GTG Cuts at: 2442 3034 2442 Size: 592 5763 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5362 809 Size: 507 4046 1802 Fragments arranged by size: 4046 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2552 2848 2925 Size: 54 1425 50 275 18 722 296 77 Cuts at: 2925 3167 3802 4691 6320 8 Size: 242 635 889 1629 43 Fragments arranged by size: 1629 1425 889 722 635 296 275 242 77 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3802 1537 Size: 275 1990 4090 Fragments arranged by size: 4090 1990 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3273 4285 1353 Size: 1920 1012 3423 Fragments arranged by size: 3423 1920 1012 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4942 5316 5462 387 Size: 1064 138 3353 374 146 1280 Fragments arranged by size: 3353 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3178 3178 Size: 6355 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2348 2432 2572 2662 2677 3173 3583 Size: 393 716 84 140 90 15 496 410 Cuts at: 3583 4418 5015 5802 6070 1239 Size: 835 597 787 268 1524 Fragments arranged by size: 1524 835 787 716 597 496 410 393 268 140 90 84 15 EcoNI CCTnn'n_nnAGG Cuts at: 2612 2612 Size: 6355 EcoO109I rG'GnC_Cy Cuts at: 2203 3391 2203 Size: 1188 5167 EcoRI G'AATT_C Cuts at: 3664 4042 3664 Size: 378 5977 EcoRV GAT'ATC Cuts at: 2653 2653 Size: 6355 FspI TGC'GCA Cuts at: 2578 4861 2578 Size: 2283 4072 HaeII r_GCGC'y Cuts at: 1678 3479 3578 5502 1678 Size: 1801 99 1924 2531 Fragments arranged by size: 2531 1924 1801 99 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2023 2055 2780 2812 3037 Size: 32 135 32 451 32 725 32 225 Cuts at: 3037 3069 4996 5028 5070 5102 1373 Size: 32 1927 32 42 32 2626 Fragments arranged by size: 2626 1927 725 451 225 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3270 3699 3945 669 Size: 988 281 1332 429 246 3079 Fragments arranged by size: 3079 1332 988 429 281 246 HindIII A'AGCT_T Cuts at: 748 2473 748 Size: 1725 4630 HpaI GTT'AAC Cuts at: 3945 3945 Size: 6355 KpnI G_GTAC'C Cuts at: 3680 3680 Size: 6355 MfeI C'AATT_G Cuts at: 3954 3954 Size: 6355 MluI A'CGCG_T Cuts at: 6231 6231 Size: 6355 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2298 5469 5653 1305 Size: 993 3171 184 2007 Fragments arranged by size: 3171 2007 993 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2554 2850 2927 3169 10 Size: 54 1425 343 722 296 77 242 3196 Fragments arranged by size: 3196 1425 722 343 296 242 77 54 MslI CAynn'nnrTG Cuts at: 518 1166 3617 4313 4672 4831 518 Size: 648 2451 696 359 159 2042 Fragments arranged by size: 2451 2042 696 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2968 3383 3771 4448 5596 5841 1606 Size: 1362 415 388 677 1148 245 2120 Fragments arranged by size: 2120 1362 1148 677 415 388 245 NcoI C'CATG_G Cuts at: 513 1065 2262 2988 513 Size: 552 1197 726 3880 Fragments arranged by size: 3880 1197 726 552 NdeI CA'TA_TG Cuts at: 387 2634 2672 387 Size: 2247 38 4070 Fragments arranged by size: 4070 2247 38 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6355 NotI GC'GGCC_GC Cuts at: 3802 3802 Size: 6355 NspI r_CATG'y Cuts at: 1132 1229 2386 3016 3713 5258 1132 Size: 97 1157 630 697 1545 2229 Fragments arranged by size: 2229 1545 1157 697 630 97 PasI CC'CwG_GG Cuts at: 1348 3355 1348 Size: 2007 4348 PciI A'CATG_T Cuts at: 5254 5254 Size: 6355 PflMI CCAn_nnn'nTGG Cuts at: 1108 2268 2923 3034 1108 Size: 1160 655 111 4429 Fragments arranged by size: 4429 1160 655 111 PmeI GTTT'AAAC Cuts at: 3659 3659 Size: 6355 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4424 4456 5937 5969 4424 Size: 32 1481 32 4810 Fragments arranged by size: 4810 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2203 3391 2203 Size: 1188 5167 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6355 PsiI TTA'TAA Cuts at: 3925 3925 Size: 6355 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3085 3117 3085 Size: 32 6323 PstI C_TGCA'G Cuts at: 830 2937 3000 3630 3707 830 Size: 2107 63 630 77 3478 Fragments arranged by size: 3478 2107 630 77 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6355 PvuII CAG'CTG Cuts at: 2968 2968 Size: 6355 SacI G_AGCT'C Cuts at: 3180 3180 Size: 6355 SalI G'TCGA_C Cuts at: 1936 3697 1936 Size: 1761 4594 SbfI CC_TGCA'GG Cuts at: 3707 3707 Size: 6355 ScaI AGT'ACT Cuts at: 4603 6315 4603 Size: 1712 4643 SfcI C'TryA_G Cuts at: 826 1045 2933 2996 3626 3703 4838 5519 5710 Size: 219 1888 63 630 77 1135 681 191 Cuts at: 5710 826 Size: 1471 Fragments arranged by size: 1888 1471 1135 681 630 219 191 77 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6355 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6355 SmaI CCC'GGG Cuts at: 1872 3682 1872 Size: 1810 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4459 5360 5622 820 Size: 197 926 2516 901 262 1553 Fragments arranged by size: 2516 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4146 493 Size: 3653 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6355 SphI G_CATG'C Cuts at: 1132 2386 3713 1132 Size: 1254 1327 3774 Fragments arranged by size: 3774 1327 1254 SspI AAT'ATT Cuts at: 5 52 2497 4279 5 Size: 47 2445 1782 2081 Fragments arranged by size: 2445 2081 1782 47 StuI AGG'CCT Cuts at: 1517 3596 1517 Size: 2079 4276 StyI C'CwwG_G Cuts at: 513 1065 2025 2262 2613 2988 3794 513 Size: 552 960 237 351 375 806 3074 Fragments arranged by size: 3074 960 806 552 375 351 237 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2254 2596 2651 4382 4399 4552 4737 Size: 1255 674 342 55 1731 17 153 185 Cuts at: 4737 5182 325 Size: 445 1498 Fragments arranged by size: 1731 1498 1255 674 445 342 185 153 55 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4601 6313 96 Size: 274 80 33 51 1443 2624 1712 138 Fragments arranged by size: 2624 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6315 6347 6315 Size: 32 6323 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6355 XbaI T'CTAG_A Cuts at: 3691 3691 Size: 6355 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3014 3073 3119 3412 3014 Size: 59 46 293 5957 Fragments arranged by size: 5957 293 59 46 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6355 XmaI C'CCGG_G Cuts at: 1870 3680 1870 Size: 1810 4545 XmnI GAAnn'nnTTC Cuts at: 4484 4484 Size: 6355 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3544 4163 276 Size: 53 83 186 936 2010 619 2468 Fragments arranged by size: 2468 2010 936 619 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI