(Circular) (Six-base) MAPSORT of: pFN21AA1953.seq Check: 5214 from: 1 to: 7531 pFN21AA1953 7531 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3588 3588 Size: 7531 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2796 5341 278 Size: 53 83 186 936 1260 2545 2468 Fragments arranged by size: 2545 2468 1260 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2177 4874 1937 Size: 240 2697 4594 Fragments arranged by size: 4594 2697 240 Acc65I G'GTAC_C Cuts at: 4852 4852 Size: 7531 AclI AA'CG_TT Cuts at: 1563 5658 6031 1563 Size: 4095 373 3063 Fragments arranged by size: 4095 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2259 3319 3647 4595 5594 6978 7246 1632 Size: 627 1060 328 948 999 1384 268 1917 Fragments arranged by size: 1917 1384 1060 999 948 627 328 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7334 AflIII A'CryG_T Cuts at: 1203 6430 7407 1203 Size: 5227 977 1327 Fragments arranged by size: 5227 1327 977 AgeI A'CCGG_T Cuts at: 3943 6362 3943 Size: 2419 5112 AhdI GACnn_n'nnGTC Cuts at: 2105 6260 2105 Size: 4155 3376 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7531 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2546 2871 4241 1184 Size: 1362 325 1370 4474 Fragments arranged by size: 4474 1370 1362 325 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2240 2272 2240 Size: 32 7499 AlwNI CAG_nnn'CTG Cuts at: 2137 2545 3826 4515 4540 5274 6846 2137 Size: 408 1281 689 25 734 1572 2822 Fragments arranged by size: 2822 1572 1281 734 689 408 25 ApaI G_GGCC'C Cuts at: 3133 3820 4581 3133 Size: 687 761 6083 Fragments arranged by size: 6083 761 687 ApaLI G'TGCA_C Cuts at: 2846 3386 5588 6744 2846 Size: 540 2202 1156 3633 Fragments arranged by size: 3633 2202 1156 540 ApoI r'AATT_y Cuts at: 1493 2162 2763 3614 4840 5066 5218 7327 1493 Size: 669 601 851 1226 226 152 2109 1697 Fragments arranged by size: 2109 1697 1226 851 669 601 226 152 AseI AT'TA_AT Cuts at: 160 1032 6085 160 Size: 872 5053 1606 Fragments arranged by size: 5053 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7531 AvaI C'yCGr_G Cuts at: 1870 1943 2110 3273 3285 4112 4271 4856 1870 Size: 73 167 1163 12 827 159 585 4545 Fragments arranged by size: 4545 1163 827 585 167 159 73 12 BamHI G'GATC_C Cuts at: 4861 5228 4861 Size: 367 7164 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2499 4183 4453 4852 6307 Size: 325 217 627 712 1684 270 399 1455 Cuts at: 6307 618 Size: 1842 Fragments arranged by size: 1842 1684 1455 712 627 399 325 270 217 BanII G_rGCy'C Cuts at: 1391 3133 3767 3820 4412 4581 4830 4850 1391 Size: 1742 634 53 592 169 249 20 4072 Fragments arranged by size: 4072 1742 634 592 249 169 53 20 BbeI G_GCGC'C Cuts at: 2503 4457 2503 Size: 1954 5577 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2247 3202 3635 4572 928 Size: 971 348 955 433 937 3887 Fragments arranged by size: 3887 971 955 937 433 348 BbvCI CC'TCA_GC Cuts at: 3872 3872 Size: 7531 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5722 5756 1903 Size: 34 50 34 3701 34 3678 Fragments arranged by size: 3701 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5423 6633 5423 Size: 1210 6321 BclI T'GATC_A Cuts at: 1548 4138 1548 Size: 2590 4941 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3646 6142 136 Size: 107 122 71 631 605 1974 2496 1525 Fragments arranged by size: 2496 1974 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4262 7526 4262 Size: 3264 4267 BlpI GC'TnA_GC Cuts at: 2558 3761 4948 2558 Size: 1203 1187 5141 Fragments arranged by size: 5141 1203 1187 Bme1580I G_kGCm'C Cuts at: 1790 2850 3133 3390 3820 4186 4581 5592 6748 Size: 1060 283 257 430 366 395 1011 1156 Cuts at: 6748 1790 Size: 2573 Fragments arranged by size: 2573 1156 1060 1011 430 395 366 283 257 BmgBI CAC'GTC Cuts at: 1318 2569 3328 1318 Size: 1251 759 5521 Fragments arranged by size: 5521 1251 759 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4658 5262 6220 Size: 416 53 83 399 24 3241 604 958 Cuts at: 6220 442 Size: 1753 Fragments arranged by size: 3241 1753 958 604 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7531 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2600 2624 3042 3129 3365 3380 3711 3754 Size: 1361 24 418 87 236 15 331 43 Cuts at: 3754 3923 4382 6191 1239 Size: 169 459 1809 2579 Fragments arranged by size: 2579 1809 1361 459 418 331 236 169 87 43 24 15 Bpu10I CC'TnA_GC Cuts at: 3872 4073 3872 Size: 201 7330 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3239 3977 5656 6521 6819 3239 Size: 738 1679 865 298 3951 Fragments arranged by size: 3951 1679 865 738 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3180 4138 6194 882 Size: 617 1681 958 2056 2219 Fragments arranged by size: 2219 2056 1681 958 617 BsaAI yAC'GTr Cuts at: 493 1204 3166 3586 5322 493 Size: 711 1962 420 1736 2702 Fragments arranged by size: 2702 1962 1736 711 420 BsaBI GATnn'nnATC Cuts at: 4044 7525 4044 Size: 3481 4050 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2500 2793 4454 5338 Size: 53 83 186 936 967 293 1661 884 Cuts at: 5338 5720 6355 275 Size: 382 635 1451 Fragments arranged by size: 1661 1451 967 936 884 635 382 293 186 83 53 BsaWI w'CCGG_w Cuts at: 3018 3189 3790 3943 5964 6362 6636 6783 7194 Size: 171 601 153 2021 398 274 147 411 Cuts at: 7194 3018 Size: 3355 Fragments arranged by size: 3355 2021 601 411 398 274 171 153 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2304 2334 3338 3368 4305 Size: 30 165 30 901 30 1004 30 937 Cuts at: 4305 4335 1178 Size: 30 4374 Fragments arranged by size: 4374 1004 937 901 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2498 3143 3407 3410 3440 3920 3978 1186 Size: 1312 645 264 3 30 480 58 4739 Fragments arranged by size: 4739 1312 645 480 264 58 30 3 BseYI C'CCAG_C Cuts at: 2017 2073 2447 2538 3479 4353 4528 4580 6734 Size: 56 374 91 941 874 175 52 2154 Cuts at: 6734 7230 2017 Size: 496 2318 Fragments arranged by size: 2318 2154 941 874 496 374 175 91 56 52 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3512 3566 3971 1163 Size: 573 1776 54 405 4723 Fragments arranged by size: 4723 1776 573 405 54 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3237 4981 5742 6770 1540 Size: 275 186 1236 1744 761 1028 2301 Fragments arranged by size: 2301 1744 1236 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2520 2850 3390 3767 4323 4830 5592 5677 6748 Size: 330 540 377 556 507 762 85 1071 Cuts at: 6748 2520 Size: 3303 Fragments arranged by size: 3303 1071 762 556 540 507 377 330 85 BsmI GAATG_Cn' Cuts at: 2226 5042 5135 2226 Size: 2816 93 4622 Fragments arranged by size: 4622 2816 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3236 3734 3236 Size: 498 7033 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2520 2850 3133 3390 3767 3820 4186 Size: 399 730 330 283 257 377 53 366 Cuts at: 4186 4323 4412 4581 4830 4850 5592 5677 6748 Size: 137 89 169 249 20 742 85 1071 Cuts at: 6748 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 730 399 377 366 330 283 257 249 169 137 89 85 53 20 BspEI T'CCGG_A Cuts at: 7194 7194 Size: 7531 BspHI T'CATG_A Cuts at: 5420 7150 5420 Size: 1730 5801 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3588 4886 844 Size: 2744 1298 3489 Fragments arranged by size: 3489 2744 1298 BsrBI CCG'CTC Cuts at: 1394 1823 3448 3802 5418 1394 Size: 429 1625 354 1616 3507 Fragments arranged by size: 3507 1625 1616 429 354 BsrDI GCAATG_nn' Cuts at: 65 1248 3543 4485 6026 6200 65 Size: 1183 2295 942 1541 174 1396 Fragments arranged by size: 2295 1541 1396 1183 942 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3880 3943 4450 6175 6362 1700 Size: 110 2070 63 507 1725 187 2869 Fragments arranged by size: 2869 2070 1725 507 187 110 63 BsrGI T'GTAC_A Cuts at: 96 3084 96 Size: 2988 4543 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7531 BssSI C'ACGA_G Cuts at: 3389 5591 6603 3389 Size: 2202 1012 4317 Fragments arranged by size: 4317 2202 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3958 794 Size: 3164 4367 BstBI TT'CG_AA Cuts at: 6335 6335 Size: 7531 BstEII G'GTnAC_C Cuts at: 1184 3592 1184 Size: 2408 5123 BstXI CCAn_nnnn'nTGG Cuts at: 2890 3487 4671 2890 Size: 597 1184 5750 Fragments arranged by size: 5750 1184 597 BstYI r'GATC_y Cuts at: 1972 3258 4262 4861 5228 5613 5630 7082 7168 Size: 1286 1004 599 367 385 17 1452 86 Cuts at: 7168 7180 7526 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1286 1004 599 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4124 4629 4124 Size: 505 7026 BtgI C'CryG_G Cuts at: 513 1065 3231 3913 3930 4519 513 Size: 552 2166 682 17 589 3525 Fragments arranged by size: 3525 2166 682 589 552 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2578 3711 487 Size: 683 56 1352 1133 4307 Fragments arranged by size: 4307 1352 1133 683 56 BtsI GCAGTG_nn' Cuts at: 2445 2647 2917 5047 5841 5861 2445 Size: 202 270 2130 794 20 4115 Fragments arranged by size: 4115 2130 794 270 202 20 ClaI AT'CG_AT Cuts at: 1554 3468 1554 Size: 1914 5617 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7496 DraI TTT'AAA Cuts at: 2722 4835 2722 Size: 2113 5418 DraIII CAC_nnn'GTG Cuts at: 4089 4089 Size: 7531 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4154 4530 6538 809 Size: 507 2838 376 2008 1802 Fragments arranged by size: 2838 2008 1802 507 376 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4201 4807 4978 Size: 54 1425 50 275 18 2371 606 171 Cuts at: 4978 5867 7496 8 Size: 889 1629 43 Fragments arranged by size: 2371 1629 1425 889 606 275 171 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4978 1537 Size: 275 3166 4090 Fragments arranged by size: 4090 3166 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2055 2089 2348 2963 4695 5461 1353 Size: 702 34 259 615 1732 766 3423 Fragments arranged by size: 3423 1732 766 702 615 259 34 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6118 6492 6638 387 Size: 1064 138 4529 374 146 1280 Fragments arranged by size: 4529 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3765 4828 3765 Size: 1063 6468 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2259 2600 2624 3042 3129 3319 3365 Size: 393 627 341 24 418 87 190 46 Cuts at: 3365 3380 3647 3711 3754 3923 4382 4595 5594 Size: 15 267 64 43 169 459 213 999 Cuts at: 5594 6191 6978 7246 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 999 787 627 597 459 418 393 341 268 267 213 190 169 87 64 46 43 24 15 EcoNI CCTnn'n_nnAGG Cuts at: 3905 4544 3905 Size: 639 6892 EcoO109I rG'GnC_Cy Cuts at: 2107 3129 3130 3441 3823 3935 3983 4501 4577 Size: 1022 1 311 382 112 48 518 76 Cuts at: 4577 4625 2107 Size: 48 5013 Fragments arranged by size: 5013 1022 518 382 311 112 76 48 48 1 EcoRI G'AATT_C Cuts at: 2162 4840 5218 2162 Size: 2678 378 4475 Fragments arranged by size: 4475 2678 378 EcoRV GAT'ATC Cuts at: 3048 3048 Size: 7531 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3058 3058 Size: 7531 FspI TGC'GCA Cuts at: 4229 6037 4229 Size: 1808 5723 HaeII r_GCGC'y Cuts at: 1678 2503 4457 6678 1678 Size: 825 1954 2221 2531 Fragments arranged by size: 2531 2221 1954 825 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2091 2123 2763 2778 2795 Size: 32 135 32 519 32 640 15 17 Cuts at: 2795 2810 3279 3311 3338 3370 3392 3424 3975 Size: 15 469 32 27 32 22 32 551 Cuts at: 3975 4007 4252 4254 4284 4286 4305 4337 6172 Size: 32 245 2 30 2 19 32 1835 Cuts at: 6172 6204 6246 6278 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1835 640 551 519 469 245 135 42 32 32 32 32 32 32 32 32 32 32 30 27 22 19 17 15 15 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 4875 5121 669 Size: 988 281 2937 246 3079 Fragments arranged by size: 3079 2937 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7531 HpaI GTT'AAC Cuts at: 5121 5121 Size: 7531 KasI G'GCGC_C Cuts at: 2499 4453 2499 Size: 1954 5577 KpnI G_GTAC'C Cuts at: 4856 4856 Size: 7531 MfeI C'AATT_G Cuts at: 5130 5130 Size: 7531 MluI A'CGCG_T Cuts at: 7407 7407 Size: 7531 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3004 3039 6645 6829 1305 Size: 1699 35 3606 184 2007 Fragments arranged by size: 3606 2007 1699 184 35 MscI TGG'CCA Cuts at: 10 64 1489 1832 4203 4809 10 Size: 54 1425 343 2371 606 2732 Fragments arranged by size: 2732 2371 1425 606 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2528 3863 4796 5489 5848 6007 518 Size: 648 1362 1335 933 693 359 159 2042 Fragments arranged by size: 2042 1362 1335 933 693 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2276 2545 3814 4947 5624 6772 7017 1606 Size: 670 269 1269 1133 677 1148 245 2120 Fragments arranged by size: 2120 1269 1148 1133 677 670 269 245 NaeI GCC'GGC Cuts at: 4452 4452 Size: 7531 NarI GG'CG_CC Cuts at: 2500 4454 2500 Size: 1954 5577 NcoI C'CATG_G Cuts at: 513 1065 3930 513 Size: 552 2865 4114 Fragments arranged by size: 4114 2865 552 NdeI CA'TA_TG Cuts at: 387 3096 387 Size: 2709 4822 NgoMIV G'CCGG_C Cuts at: 4450 4450 Size: 7531 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7531 NotI GC'GGCC_GC Cuts at: 4978 4978 Size: 7531 NspI r_CATG'y Cuts at: 1132 1229 3922 4889 6434 1132 Size: 97 2693 967 1545 2229 Fragments arranged by size: 2693 2229 1545 967 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7531 PciI A'CATG_T Cuts at: 6430 6430 Size: 7531 PflMI CCAn_nnn'nTGG Cuts at: 1108 3826 4339 1108 Size: 2718 513 4300 Fragments arranged by size: 4300 2718 513 PfoI T'CCnGG_A Cuts at: 2549 2577 2549 Size: 28 7503 PmeI GTTT'AAAC Cuts at: 4835 4835 Size: 7531 PmlI CAC'GTG Cuts at: 3586 3586 Size: 7531 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5600 5632 7113 7145 5600 Size: 32 1481 32 5986 Fragments arranged by size: 5986 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2107 3441 3935 3983 2107 Size: 1334 494 48 5655 Fragments arranged by size: 5655 1334 494 48 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7531 PsiI TTA'TAA Cuts at: 5101 5101 Size: 7531 PspOMI G'GGCC_C Cuts at: 3129 3816 4577 3129 Size: 687 761 6083 Fragments arranged by size: 6083 761 687 PstI C_TGCA'G Cuts at: 830 2219 2281 2440 2496 3138 4576 4883 830 Size: 1389 62 159 56 642 1438 307 3478 Fragments arranged by size: 3478 1438 1389 642 307 159 62 56 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7531 PvuII CAG'CTG Cuts at: 2276 2545 2276 Size: 269 7262 SacI G_AGCT'C Cuts at: 3767 4830 3767 Size: 1063 6468 SalI G'TCGA_C Cuts at: 1936 4873 1936 Size: 2937 4594 SanDI GG'GwC_CC Cuts at: 2107 3935 2107 Size: 1828 5703 SapI GCTCTTCn'nnn_ Cuts at: 4695 4695 Size: 7531 SbfI CC_TGCA'GG Cuts at: 4576 4883 4576 Size: 307 7224 ScaI AGT'ACT Cuts at: 5779 7491 5779 Size: 1712 5819 SfcI C'TryA_G Cuts at: 826 1045 2215 2277 2436 2492 2730 3134 4572 Size: 219 1170 62 159 56 238 404 1438 Cuts at: 4572 4879 6014 6695 6886 826 Size: 307 1135 681 191 1471 Fragments arranged by size: 1471 1438 1170 1135 681 404 307 238 219 191 159 62 56 SfoI GGC'GCC Cuts at: 2501 4455 2501 Size: 1954 5577 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7531 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7531 SmaI CCC'GGG Cuts at: 1872 4114 4858 1872 Size: 2242 744 4545 Fragments arranged by size: 4545 2242 744 SmlI C'TyrA_G Cuts at: 820 1017 1943 3254 3992 4271 5635 6536 6798 Size: 197 926 1311 738 279 1364 901 262 Cuts at: 6798 820 Size: 1553 Fragments arranged by size: 1553 1364 1311 926 901 738 279 262 197 SnaBI TAC'GTA Cuts at: 493 5322 493 Size: 4829 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7531 SphI G_CATG'C Cuts at: 1132 4889 1132 Size: 3757 3774 SspI AAT'ATT Cuts at: 5 52 2424 5455 5 Size: 47 2372 3031 2081 Fragments arranged by size: 3031 2372 2081 47 StuI AGG'CCT Cuts at: 1517 3688 1517 Size: 2171 5360 StyI C'CwwG_G Cuts at: 513 1065 2946 3930 4970 513 Size: 552 1881 984 1040 3074 Fragments arranged by size: 3074 1881 1040 984 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3118 5558 5575 5728 5913 6358 325 Size: 1255 1538 2440 17 153 185 445 1498 Fragments arranged by size: 2440 1538 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2734 3084 4758 Size: 274 80 33 51 1443 757 350 1674 Cuts at: 4758 5777 7489 96 Size: 1019 1712 138 Fragments arranged by size: 1712 1674 1443 1019 757 350 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2304 2336 7491 7523 2304 Size: 32 5155 32 2312 Fragments arranged by size: 5155 2312 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7531 XbaI T'CTAG_A Cuts at: 4867 4867 Size: 7531 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2831 3179 2831 Size: 348 7183 XhoI C'TCGA_G Cuts at: 1943 4271 1943 Size: 2328 5203 XmaI C'CCGG_G Cuts at: 1870 4112 4856 1870 Size: 2242 744 4545 Fragments arranged by size: 4545 2242 744 XmnI GAAnn'nnTTC Cuts at: 5660 5660 Size: 7531 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2794 5339 276 Size: 53 83 186 936 1260 2545 2468 Fragments arranged by size: 2545 2468 1260 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI