(Circular) (Six-base) MAPSORT of: pFN21AA1948.seq Check: 814 from: 1 to: 6301 pFN21AA1948 6301 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4111 278 Size: 53 83 186 936 2575 2468 Fragments arranged by size: 2575 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3644 1937 Size: 1707 4594 Acc65I G'GTAC_C Cuts at: 3622 3622 Size: 6301 AclI AA'CG_TT Cuts at: 1563 4428 4801 1563 Size: 2865 373 3063 Fragments arranged by size: 3063 2865 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2401 2608 3280 4364 5748 6016 1632 Size: 769 207 672 1084 1384 268 1917 Fragments arranged by size: 1917 1384 1084 769 672 268 207 AflII C'TTAA_G Cuts at: 820 1017 2339 820 Size: 197 1322 4782 Fragments arranged by size: 4782 1322 197 AflIII A'CryG_T Cuts at: 1203 5200 6177 1203 Size: 3997 977 1327 Fragments arranged by size: 3997 1327 977 AgeI A'CCGG_T Cuts at: 5132 5132 Size: 6301 AhdI GACnn_n'nnGTC Cuts at: 5030 5030 Size: 6301 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6301 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6301 AlwNI CAG_nnn'CTG Cuts at: 4044 5616 4044 Size: 1572 4729 ApaLI G'TGCA_C Cuts at: 4358 5514 4358 Size: 1156 5145 ApoI r'AATT_y Cuts at: 1493 2293 2574 3078 3162 3498 3582 3610 3836 Size: 800 281 504 84 336 84 28 226 Cuts at: 3836 3988 6097 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 800 504 336 281 226 152 84 84 28 AseI AT'TA_AT Cuts at: 160 1032 4855 160 Size: 872 3823 1606 Fragments arranged by size: 3823 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 AvaI C'yCGr_G Cuts at: 1870 1943 3626 1870 Size: 73 1683 4545 Fragments arranged by size: 4545 1683 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2404 2437 2404 Size: 33 6268 BamHI G'GATC_C Cuts at: 3631 3998 3631 Size: 367 5934 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3622 5077 618 Size: 325 217 627 1835 1455 1842 Fragments arranged by size: 1842 1835 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3620 1391 Size: 2229 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2227 928 Size: 971 328 5002 Fragments arranged by size: 5002 971 328 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4492 4526 1903 Size: 34 2555 34 3678 Fragments arranged by size: 3678 2555 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2047 2405 4193 5403 2047 Size: 358 1788 1210 2945 Fragments arranged by size: 2945 1788 1210 358 BclI T'GATC_A Cuts at: 1548 1548 Size: 6301 BfrBI ATG'CAT Cuts at: 2746 2746 Size: 6301 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4912 136 Size: 107 122 71 631 605 3240 1525 Fragments arranged by size: 3240 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2722 6296 2722 Size: 3574 2727 BlpI GC'TnA_GC Cuts at: 3718 3718 Size: 6301 Bme1580I G_kGCm'C Cuts at: 1790 4362 5518 1790 Size: 2572 1156 2573 Fragments arranged by size: 2573 2572 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6301 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2147 2296 4032 Size: 416 53 83 399 24 730 149 1736 Cuts at: 4032 4990 442 Size: 958 1753 Fragments arranged by size: 1753 1736 958 730 416 399 149 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6301 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2037 4961 1239 Size: 798 2924 2579 Fragments arranged by size: 2924 2579 798 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2388 4426 5291 5589 2388 Size: 2038 865 298 3100 Fragments arranged by size: 3100 2038 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2137 2234 2246 2351 2392 4964 882 Size: 617 638 97 12 105 41 2572 2219 Fragments arranged by size: 2572 2219 638 617 105 97 41 12 BsaAI yAC'GTr Cuts at: 493 1204 4092 493 Size: 711 2888 2702 Fragments arranged by size: 2888 2702 711 BsaBI GATnn'nnATC Cuts at: 6295 6295 Size: 6301 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4108 4490 5125 275 Size: 53 83 186 936 2575 382 635 1451 Fragments arranged by size: 2575 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4734 5132 5406 5553 5964 4734 Size: 398 274 147 411 5071 Fragments arranged by size: 5071 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2231 2261 3051 3081 1178 Size: 30 165 30 828 30 790 30 4398 Fragments arranged by size: 4398 828 790 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6301 BseYI C'CCAG_C Cuts at: 5504 6000 5504 Size: 496 5805 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2619 2739 1163 Size: 573 883 120 4725 Fragments arranged by size: 4725 883 573 120 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3751 4512 5540 1540 Size: 275 186 1750 761 1028 2301 Fragments arranged by size: 2301 1750 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4362 4447 5518 4362 Size: 85 1071 5145 Fragments arranged by size: 5145 1071 85 BsmI GAATG_Cn' Cuts at: 2942 3035 3203 3287 3414 3812 3905 2942 Size: 93 168 84 127 398 93 5338 Fragments arranged by size: 5338 398 168 127 93 93 84 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3620 4362 4447 5518 1391 Size: 399 1830 742 85 1071 2174 Fragments arranged by size: 2174 1830 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5964 5964 Size: 6301 BspHI T'CATG_A Cuts at: 4190 5920 4190 Size: 1730 4571 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3656 844 Size: 2812 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4188 1394 Size: 429 2365 3507 Fragments arranged by size: 3507 2365 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4796 4970 65 Size: 1183 3548 174 1396 Fragments arranged by size: 3548 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4945 5132 1700 Size: 110 3135 187 2869 Fragments arranged by size: 3135 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2094 96 Size: 1998 4303 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6301 BssSI C'ACGA_G Cuts at: 4361 5373 4361 Size: 1012 5289 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6301 BstBI TT'CG_AA Cuts at: 5105 5105 Size: 6301 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6301 BstXI CCAn_nnnn'nTGG Cuts at: 2061 2061 Size: 6301 BstYI r'GATC_y Cuts at: 1972 2722 3631 3998 4383 4400 5852 5938 5950 Size: 750 909 367 385 17 1452 86 12 Cuts at: 5950 6296 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 909 750 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2332 3133 513 Size: 552 939 328 801 3681 Fragments arranged by size: 3681 939 801 552 328 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5562 Fragments arranged by size: 5562 683 56 BtsI GCAGTG_nn' Cuts at: 3817 4611 4631 3817 Size: 794 20 5487 Fragments arranged by size: 5487 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6301 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2929 2964 529 Size: 35 2365 35 3866 Fragments arranged by size: 3866 2365 35 35 DraI TTT'AAA Cuts at: 2355 3605 2355 Size: 1250 5051 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5308 809 Size: 507 3992 1802 Fragments arranged by size: 3992 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3748 4637 6266 Size: 54 1425 50 275 18 1918 889 1629 Cuts at: 6266 8 Size: 43 Fragments arranged by size: 1918 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3748 1537 Size: 275 1936 4090 Fragments arranged by size: 4090 1936 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2052 4231 1353 Size: 699 2179 3423 Fragments arranged by size: 3423 2179 699 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4888 5262 5408 387 Size: 1064 138 3299 374 146 1280 Fragments arranged by size: 3299 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2037 2401 2608 3280 4364 4961 5748 Size: 393 405 364 207 672 1084 597 787 Cuts at: 5748 6016 1239 Size: 268 1524 Fragments arranged by size: 1524 1084 787 672 597 405 393 364 268 207 EcoNI CCTnn'n_nnAGG Cuts at: 2406 2406 Size: 6301 EcoRI G'AATT_C Cuts at: 2574 3078 3162 3498 3610 3988 2574 Size: 504 84 336 112 378 4887 Fragments arranged by size: 4887 504 378 336 112 84 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3562 3562 Size: 6301 FspI TGC'GCA Cuts at: 4807 4807 Size: 6301 HaeII r_GCGC'y Cuts at: 1678 5448 1678 Size: 3770 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2354 2386 2887 2919 4942 Size: 32 135 32 782 32 501 32 2023 Cuts at: 4942 4974 5016 5048 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2023 782 501 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3645 3891 669 Size: 988 281 1707 246 3079 Fragments arranged by size: 3079 1707 988 281 246 HindIII A'AGCT_T Cuts at: 748 2703 2909 748 Size: 1955 206 4140 Fragments arranged by size: 4140 1955 206 HpaI GTT'AAC Cuts at: 3891 3891 Size: 6301 KpnI G_GTAC'C Cuts at: 3626 3626 Size: 6301 MfeI C'AATT_G Cuts at: 3900 3900 Size: 6301 MluI A'CGCG_T Cuts at: 6177 6177 Size: 6301 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2091 5415 5599 1305 Size: 786 3324 184 2007 Fragments arranged by size: 3324 2007 786 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4479 Fragments arranged by size: 4479 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2140 2583 2667 2751 2835 3003 3087 Size: 648 974 443 84 84 84 168 84 Cuts at: 3087 3171 3255 3339 3507 4259 4618 4777 518 Size: 84 84 84 168 752 359 159 2042 Fragments arranged by size: 2042 974 752 648 443 359 168 168 159 84 84 84 84 84 84 84 MspA1I CmG'CkG Cuts at: 1606 3717 4394 5542 5787 1606 Size: 2111 677 1148 245 2120 Fragments arranged by size: 2120 2111 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2332 513 Size: 552 939 328 4482 Fragments arranged by size: 4482 939 552 328 NdeI CA'TA_TG Cuts at: 387 2711 387 Size: 2324 3977 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6301 NotI GC'GGCC_GC Cuts at: 3748 3748 Size: 6301 NsiI A_TGCA'T Cuts at: 2748 2748 Size: 6301 NspI r_CATG'y Cuts at: 1132 1229 3659 5204 1132 Size: 97 2430 1545 2229 Fragments arranged by size: 2430 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6301 PciI A'CATG_T Cuts at: 5200 5200 Size: 6301 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6301 PmeI GTTT'AAAC Cuts at: 3605 3605 Size: 6301 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4370 4402 5883 5915 4370 Size: 32 1481 32 4756 Fragments arranged by size: 4756 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6301 PsiI TTA'TAA Cuts at: 3354 3871 3354 Size: 517 5784 PstI C_TGCA'G Cuts at: 830 3653 830 Size: 2823 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6301 SalI G'TCGA_C Cuts at: 1936 3643 1936 Size: 1707 4594 SbfI CC_TGCA'GG Cuts at: 3653 3653 Size: 6301 ScaI AGT'ACT Cuts at: 4549 6261 4549 Size: 1712 4589 SfcI C'TryA_G Cuts at: 826 1045 2119 3649 4784 5465 5656 826 Size: 219 1074 1530 1135 681 191 1471 Fragments arranged by size: 1530 1471 1135 1074 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6301 SmaI CCC'GGG Cuts at: 1872 3628 1872 Size: 1756 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2339 2403 4405 5306 5568 820 Size: 197 926 396 64 2002 901 262 1553 Fragments arranged by size: 2002 1553 926 901 396 262 197 64 SnaBI TAC'GTA Cuts at: 493 4092 493 Size: 3599 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6301 SphI G_CATG'C Cuts at: 1132 3659 1132 Size: 2527 3774 SspI AAT'ATT Cuts at: 5 52 4225 5 Size: 47 4173 2081 Fragments arranged by size: 4173 2081 47 StuI AGG'CCT Cuts at: 1517 2957 3041 3293 3377 3461 3545 1517 Size: 1440 84 252 84 84 84 4273 Fragments arranged by size: 4273 1440 252 84 84 84 84 StyI C'CwwG_G Cuts at: 513 1065 2004 2195 2332 3740 513 Size: 552 939 191 137 1408 3074 Fragments arranged by size: 3074 1408 939 552 191 137 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4328 4345 4498 4683 5128 325 Size: 1255 2748 17 153 185 445 1498 Fragments arranged by size: 2748 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2094 2731 2983 Size: 274 80 33 51 1443 117 637 252 Cuts at: 2983 4547 6259 96 Size: 1564 1712 138 Fragments arranged by size: 1712 1564 1443 637 274 252 138 117 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6261 6293 6261 Size: 32 6269 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6301 XbaI T'CTAG_A Cuts at: 3637 3637 Size: 6301 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6301 XmaI C'CCGG_G Cuts at: 1870 3626 1870 Size: 1756 4545 XmnI GAAnn'nnTTC Cuts at: 2537 3293 3502 4430 2537 Size: 756 209 928 4408 Fragments arranged by size: 4408 928 756 209 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4109 276 Size: 53 83 186 936 2575 2468 Fragments arranged by size: 2575 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI