(Circular) (Six-base) MAPSORT of: pFN21AA1936.seq Check: 3355 from: 1 to: 7117 pFN21AA1936 7117 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3565 3565 Size: 7117 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2069 4927 278 Size: 53 83 186 936 533 2858 2468 Fragments arranged by size: 2858 2468 936 533 186 83 53 AccI GT'mk_AC Cuts at: 1937 4460 1937 Size: 2523 4594 Acc65I G'GTAC_C Cuts at: 4438 4438 Size: 7117 AclI AA'CG_TT Cuts at: 1563 5244 5617 1563 Size: 3681 373 3063 Fragments arranged by size: 3681 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3485 5180 6564 6832 1632 Size: 1853 1695 1384 268 1917 Fragments arranged by size: 1917 1853 1695 1384 268 AfeI AGC'GCT Cuts at: 2664 2664 Size: 7117 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6920 AflIII A'CryG_T Cuts at: 1203 2316 2375 3489 6016 6993 1203 Size: 1113 59 1114 2527 977 1327 Fragments arranged by size: 2527 1327 1114 1113 977 59 AgeI A'CCGG_T Cuts at: 5948 5948 Size: 7117 AhdI GACnn_n'nnGTC Cuts at: 3006 5846 3006 Size: 2840 4277 AleI CACnn'nnGTG Cuts at: 1166 3644 1166 Size: 2478 4639 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2808 3593 3877 1184 Size: 1624 785 284 4424 Fragments arranged by size: 4424 1624 785 284 AlwNI CAG_nnn'CTG Cuts at: 2264 2563 3769 4042 4860 6432 2264 Size: 299 1206 273 818 1572 2949 Fragments arranged by size: 2949 1572 1206 818 299 273 ApaI G_GGCC'C Cuts at: 3843 3843 Size: 7117 ApaLI G'TGCA_C Cuts at: 5174 6330 5174 Size: 1156 5961 ApoI r'AATT_y Cuts at: 1493 2237 3323 4426 4652 4804 6913 1493 Size: 744 1086 1103 226 152 2109 1697 Fragments arranged by size: 2109 1697 1103 1086 744 226 152 AseI AT'TA_AT Cuts at: 160 1032 3246 5671 160 Size: 872 2214 2425 1606 Fragments arranged by size: 2425 2214 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7117 AvaI C'yCGr_G Cuts at: 1870 1943 4122 4442 1870 Size: 73 2179 320 4545 Fragments arranged by size: 4545 2179 320 73 AvrII C'CTAG_G Cuts at: 3187 3187 Size: 7117 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2396 2429 2396 Size: 33 7084 BamHI G'GATC_C Cuts at: 4447 4814 4447 Size: 367 6750 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4050 4438 5893 618 Size: 325 217 627 2263 388 1455 1842 Fragments arranged by size: 2263 1842 1455 627 388 325 217 BanII G_rGCy'C Cuts at: 1391 2778 2991 3843 4236 4359 4436 1391 Size: 1387 213 852 393 123 77 4072 Fragments arranged by size: 4072 1387 852 393 213 123 77 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3271 928 Size: 971 1372 4774 Fragments arranged by size: 4774 1372 971 BbvCI CC'TCA_GC Cuts at: 2666 3770 4305 2666 Size: 1104 535 5478 Fragments arranged by size: 5478 1104 535 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5308 5342 1903 Size: 34 3371 34 3678 Fragments arranged by size: 3678 3371 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2424 5009 6219 2424 Size: 2585 1210 3322 Fragments arranged by size: 3322 2585 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7117 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5728 136 Size: 107 122 71 631 605 4056 1525 Fragments arranged by size: 4056 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3917 4251 7112 3917 Size: 334 2861 3922 Fragments arranged by size: 3922 2861 334 BlpI GC'TnA_GC Cuts at: 4534 4534 Size: 7117 Bme1580I G_kGCm'C Cuts at: 1790 3843 5178 6334 1790 Size: 2053 1335 1156 2573 Fragments arranged by size: 2573 2053 1335 1156 BmgBI CAC'GTC Cuts at: 1318 2376 3457 3898 1318 Size: 1058 1081 441 4537 Fragments arranged by size: 4537 1081 1058 441 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3044 4848 5806 Size: 416 53 83 399 24 1627 1804 958 Cuts at: 5806 442 Size: 1753 Fragments arranged by size: 1804 1753 1627 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7117 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3084 3084 Size: 7117 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4343 5777 1239 Size: 3104 1434 2579 Fragments arranged by size: 3104 2579 1434 Bpu10I CC'TnA_GC Cuts at: 2666 3513 3770 4286 4305 2666 Size: 847 257 516 19 5478 Fragments arranged by size: 5478 847 516 257 19 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2122 2672 5242 6107 6405 2122 Size: 550 2570 865 298 2834 Fragments arranged by size: 2834 2570 865 550 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2090 3215 3780 3961 5780 882 Size: 617 591 1125 565 181 1819 2219 Fragments arranged by size: 2219 1819 1125 617 591 565 181 BsaAI yAC'GTr Cuts at: 493 1204 2447 4908 493 Size: 711 1243 2461 2702 Fragments arranged by size: 2702 2461 1243 711 BsaBI GATnn'nnATC Cuts at: 7111 7111 Size: 7117 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2066 3680 4062 4924 Size: 53 83 186 936 533 1614 382 862 Cuts at: 4924 5306 5941 275 Size: 382 635 1451 Fragments arranged by size: 1614 1451 936 862 635 533 382 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5550 5948 6222 6369 6780 5550 Size: 398 274 147 411 5887 Fragments arranged by size: 5887 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2253 2283 3882 3912 4301 Size: 30 165 30 850 30 1599 30 389 Cuts at: 4301 4331 1178 Size: 30 3964 Fragments arranged by size: 3964 1599 850 389 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2113 2196 2687 3403 4189 4322 1186 Size: 927 83 491 716 786 133 3981 Fragments arranged by size: 3981 927 786 716 491 133 83 BseYI C'CCAG_C Cuts at: 2629 6320 6816 2629 Size: 3691 496 2930 Fragments arranged by size: 3691 2930 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2804 3333 3376 3945 4307 1163 Size: 573 1068 529 43 569 362 3973 Fragments arranged by size: 3973 1068 573 569 529 362 43 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4567 5328 6356 1540 Size: 275 186 2566 761 1028 2301 Fragments arranged by size: 2566 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2778 2991 3321 3337 4089 4236 4292 4359 5178 Size: 213 330 16 752 147 56 67 819 Cuts at: 5178 5263 6334 2778 Size: 85 1071 3561 Fragments arranged by size: 3561 1071 819 752 330 213 147 85 67 56 16 BsmI GAATG_Cn' Cuts at: 3854 4628 4721 3854 Size: 774 93 6250 Fragments arranged by size: 6250 774 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2366 2366 Size: 7117 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2778 2991 3321 3337 3843 4089 4236 Size: 399 988 213 330 16 506 246 147 Cuts at: 4236 4292 4359 4436 5178 5263 6334 1391 Size: 56 67 77 742 85 1071 2174 Fragments arranged by size: 2174 1071 988 742 506 399 330 246 213 147 85 77 67 56 16 BspEI T'CCGG_A Cuts at: 6780 6780 Size: 7117 BspHI T'CATG_A Cuts at: 5006 6736 5006 Size: 1730 5387 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3565 3973 4472 844 Size: 2721 408 499 3489 Fragments arranged by size: 3489 2721 499 408 BsrBI CCG'CTC Cuts at: 1394 1823 2340 5004 1394 Size: 429 517 2664 3507 Fragments arranged by size: 3507 2664 517 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3051 5612 5786 65 Size: 1183 1803 2561 174 1396 Fragments arranged by size: 2561 1803 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5761 5948 1700 Size: 110 3951 187 2869 Fragments arranged by size: 3951 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7117 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7117 BssSI C'ACGA_G Cuts at: 5177 6189 5177 Size: 1012 6105 BstAPI GCAn_nnn'nTGC Cuts at: 794 3870 794 Size: 3076 4041 BstBI TT'CG_AA Cuts at: 5921 5921 Size: 7117 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7117 BstXI CCAn_nnnn'nTGG Cuts at: 3722 3722 Size: 7117 BstYI r'GATC_y Cuts at: 1972 2008 2722 3917 4251 4447 4814 5199 5216 Size: 36 714 1195 334 196 367 385 17 Cuts at: 5216 6668 6754 6766 7112 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1195 714 385 367 346 334 196 86 36 17 12 Bsu36I CC'TnA_GG Cuts at: 2142 3059 4138 2142 Size: 917 1079 5121 Fragments arranged by size: 5121 1079 917 BtgI C'CryG_G Cuts at: 513 1065 2004 4092 4201 4334 513 Size: 552 939 2088 109 133 3296 Fragments arranged by size: 3296 2088 939 552 133 109 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3491 3533 487 Size: 683 56 2265 42 4071 Fragments arranged by size: 4071 2265 683 56 42 BtsI GCAGTG_nn' Cuts at: 2274 3438 3715 4633 5427 5447 2274 Size: 1164 277 918 794 20 3944 Fragments arranged by size: 3944 1164 918 794 277 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7117 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7082 DraI TTT'AAA Cuts at: 2730 4421 2730 Size: 1691 5426 DraIII CAC_nnn'GTG Cuts at: 3038 4093 3038 Size: 1055 6062 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2867 3953 6124 809 Size: 507 1551 1086 2171 1802 Fragments arranged by size: 2171 1802 1551 1086 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2640 2916 4564 Size: 54 1425 50 275 18 810 276 1648 Cuts at: 4564 5453 7082 8 Size: 889 1629 43 Fragments arranged by size: 1648 1629 1425 889 810 276 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4564 1537 Size: 275 2752 4090 Fragments arranged by size: 4090 2752 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2131 2357 4241 5047 1353 Size: 778 226 1884 806 3423 Fragments arranged by size: 3423 1884 806 778 226 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3922 5704 6078 6224 387 Size: 1064 138 2333 1782 374 146 1280 Fragments arranged by size: 2333 1782 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2776 2989 4234 4357 2776 Size: 213 1245 123 5536 Fragments arranged by size: 5536 1245 213 123 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3485 4343 5180 5777 6564 6832 1239 Size: 393 1853 858 837 597 787 268 1524 Fragments arranged by size: 1853 1524 858 837 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2730 2783 3015 2730 Size: 53 232 6832 Fragments arranged by size: 6832 232 53 EcoO109I rG'GnC_Cy Cuts at: 2881 3542 3839 3970 4406 2881 Size: 661 297 131 436 5592 Fragments arranged by size: 5592 661 436 297 131 EcoRI G'AATT_C Cuts at: 4426 4804 4426 Size: 378 6739 EcoRV GAT'ATC Cuts at: 2110 2110 Size: 7117 FspI TGC'GCA Cuts at: 5623 5623 Size: 7117 HaeII r_GCGC'y Cuts at: 1678 2666 3881 6264 1678 Size: 988 1215 2383 2531 Fragments arranged by size: 2531 2383 1215 988 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2015 2047 2303 2335 2992 Size: 32 135 32 443 32 256 32 657 Cuts at: 2992 3024 4301 4333 5758 5790 5832 5864 1373 Size: 32 1277 32 1425 32 42 32 2626 Fragments arranged by size: 2626 1425 1277 657 443 256 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3066 4461 4707 669 Size: 988 281 1128 1395 246 3079 Fragments arranged by size: 3079 1395 1128 988 281 246 HindIII A'AGCT_T Cuts at: 748 2623 3110 4001 748 Size: 1875 487 891 3864 Fragments arranged by size: 3864 1875 891 487 HpaI GTT'AAC Cuts at: 4707 4707 Size: 7117 KpnI G_GTAC'C Cuts at: 4442 4442 Size: 7117 MfeI C'AATT_G Cuts at: 4716 4716 Size: 7117 MluI A'CGCG_T Cuts at: 6993 6993 Size: 7117 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4362 6231 6415 1305 Size: 3057 1869 184 2007 Fragments arranged by size: 3057 2007 1869 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2642 2918 10 Size: 54 1425 343 810 276 4209 Fragments arranged by size: 4209 1425 810 343 276 54 MslI CAynn'nnrTG Cuts at: 518 1166 3644 3720 5075 5434 5593 518 Size: 648 2478 76 1355 359 159 2042 Fragments arranged by size: 2478 2042 1355 648 359 159 76 MspA1I CmG'CkG Cuts at: 1606 3688 4039 4533 5210 6358 6603 1606 Size: 2082 351 494 677 1148 245 2120 Fragments arranged by size: 2120 2082 1148 677 494 351 245 NcoI C'CATG_G Cuts at: 513 1065 2004 4334 513 Size: 552 939 2330 3296 Fragments arranged by size: 3296 2330 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7117 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7117 NotI GC'GGCC_GC Cuts at: 4564 4564 Size: 7117 NspI r_CATG'y Cuts at: 1132 1229 2320 3493 4475 6020 1132 Size: 97 1091 1173 982 1545 2229 Fragments arranged by size: 2229 1545 1173 1091 982 97 PasI CC'CwG_GG Cuts at: 1348 2470 1348 Size: 1122 5995 PciI A'CATG_T Cuts at: 2316 3489 6016 2316 Size: 1173 2527 3417 Fragments arranged by size: 3417 2527 1173 PflMI CCAn_nnn'nTGG Cuts at: 1108 3233 1108 Size: 2125 4992 PfoI T'CCnGG_A Cuts at: 3232 4350 3232 Size: 1118 5999 PmeI GTTT'AAAC Cuts at: 4421 4421 Size: 7117 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5186 5218 6699 6731 5186 Size: 32 1481 32 5572 Fragments arranged by size: 5572 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2881 3542 3970 2881 Size: 661 428 6028 Fragments arranged by size: 6028 661 428 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7117 PsiI TTA'TAA Cuts at: 3270 4687 3270 Size: 1417 5700 PspOMI G'GGCC_C Cuts at: 3839 3839 Size: 7117 PstI C_TGCA'G Cuts at: 830 2269 2484 2568 3838 4469 830 Size: 1439 215 84 1270 631 3478 Fragments arranged by size: 3478 1439 1270 631 215 84 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7117 SacI G_AGCT'C Cuts at: 2778 2991 4236 4359 2778 Size: 213 1245 123 5536 Fragments arranged by size: 5536 1245 213 123 SalI G'TCGA_C Cuts at: 1936 4459 1936 Size: 2523 4594 SapI GCTCTTCn'nnn_ Cuts at: 4241 4241 Size: 7117 SbfI CC_TGCA'GG Cuts at: 4469 4469 Size: 7117 ScaI AGT'ACT Cuts at: 5365 7077 5365 Size: 1712 5405 SexAI A'CCwGG_T Cuts at: 3951 3951 Size: 7117 SfcI C'TryA_G Cuts at: 826 1045 2088 2215 2265 2480 2564 3628 3834 Size: 219 1043 127 50 215 84 1064 206 Cuts at: 3834 3974 4169 4401 4465 5600 6281 6472 826 Size: 140 195 232 64 1135 681 191 1471 Fragments arranged by size: 1471 1135 1064 1043 681 232 219 215 206 195 191 140 127 84 64 50 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7117 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7117 SmaI CCC'GGG Cuts at: 1872 4124 4444 1872 Size: 2252 320 4545 Fragments arranged by size: 4545 2252 320 SmlI C'TyrA_G Cuts at: 820 1017 1943 2101 2651 5221 6122 6384 820 Size: 197 926 158 550 2570 901 262 1553 Fragments arranged by size: 2570 1553 926 901 550 262 197 158 SnaBI TAC'GTA Cuts at: 493 2447 4908 493 Size: 1954 2461 2702 Fragments arranged by size: 2702 2461 1954 SpeI A'CTAG_T Cuts at: 152 152 Size: 7117 SphI G_CATG'C Cuts at: 1132 4475 1132 Size: 3343 3774 SrfI GCCC'GGGC Cuts at: 4124 4124 Size: 7117 SspI AAT'ATT Cuts at: 5 52 5041 5 Size: 47 4989 2081 Fragments arranged by size: 4989 2081 47 StuI AGG'CCT Cuts at: 1517 2302 2981 1517 Size: 785 679 5653 Fragments arranged by size: 5653 785 679 StyI C'CwwG_G Cuts at: 513 1065 2004 3187 4334 4556 513 Size: 552 939 1183 1147 222 3074 Fragments arranged by size: 3074 1183 1147 939 552 222 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3026 4178 4408 5144 5161 5314 5499 Size: 1255 1446 1152 230 736 17 153 185 Cuts at: 5499 5944 325 Size: 445 1498 Fragments arranged by size: 1498 1446 1255 1152 736 445 230 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2274 5363 7075 Size: 274 80 33 51 1443 297 3089 1712 Cuts at: 7075 96 Size: 138 Fragments arranged by size: 3089 1712 1443 297 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2253 2285 3882 3914 4193 4225 7077 7109 2253 Size: 32 1597 32 279 32 2852 32 2261 Fragments arranged by size: 2852 2261 1597 279 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7117 XbaI T'CTAG_A Cuts at: 4453 4453 Size: 7117 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4219 4219 Size: 7117 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7117 XmaI C'CCGG_G Cuts at: 1870 4122 4442 1870 Size: 2252 320 4545 Fragments arranged by size: 4545 2252 320 XmnI GAAnn'nnTTC Cuts at: 5246 5246 Size: 7117 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2067 4925 276 Size: 53 83 186 936 533 2858 2468 Fragments arranged by size: 2858 2468 936 533 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BbeI BfrBI BsiWI BstZ17I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI SfiI SfoI SwaI