(Circular) (Six-base) MAPSORT of: pFN21AA1910.seq Check: 4551 from: 1 to: 6793 pFN21AA1910 6793 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2036 4603 278 Size: 53 83 186 936 500 2567 2468 Fragments arranged by size: 2567 2468 936 500 186 83 53 AccI GT'mk_AC Cuts at: 1937 2124 4136 1937 Size: 187 2012 4594 Fragments arranged by size: 4594 2012 187 Acc65I G'GTAC_C Cuts at: 4114 4114 Size: 6793 AclI AA'CG_TT Cuts at: 1563 2058 4920 5293 1563 Size: 495 2862 373 3063 Fragments arranged by size: 3063 2862 495 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4856 6240 6508 1632 Size: 3224 1384 268 1917 Fragments arranged by size: 3224 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6596 AflIII A'CryG_T Cuts at: 1203 2119 2520 3471 5692 6669 1203 Size: 916 401 951 2221 977 1327 Fragments arranged by size: 2221 1327 977 951 916 401 AgeI A'CCGG_T Cuts at: 5624 5624 Size: 6793 AhdI GACnn_n'nnGTC Cuts at: 3316 5522 3316 Size: 2206 4587 AleI CACnn'nnGTG Cuts at: 1166 3614 3908 1166 Size: 2448 294 4051 Fragments arranged by size: 4051 2448 294 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3632 1184 Size: 2448 4345 AlwNI CAG_nnn'CTG Cuts at: 2912 4536 6108 2912 Size: 1624 1572 3597 Fragments arranged by size: 3597 1624 1572 ApaI G_GGCC'C Cuts at: 4054 4054 Size: 6793 ApaLI G'TGCA_C Cuts at: 4850 6006 4850 Size: 1156 5637 ApoI r'AATT_y Cuts at: 1493 3329 4102 4328 4480 6589 1493 Size: 1836 773 226 152 2109 1697 Fragments arranged by size: 2109 1836 1697 773 226 152 AseI AT'TA_AT Cuts at: 160 1032 3987 5347 160 Size: 872 2955 1360 1606 Fragments arranged by size: 2955 1606 1360 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6793 AvaI C'yCGr_G Cuts at: 1870 1943 2443 3321 3394 3868 4118 1870 Size: 73 500 878 73 474 250 4545 Fragments arranged by size: 4545 878 500 474 250 73 73 BamHI G'GATC_C Cuts at: 3426 4123 4490 3426 Size: 697 367 5729 Fragments arranged by size: 5729 697 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2821 4114 5569 618 Size: 325 217 627 1034 1293 1455 1842 Fragments arranged by size: 1842 1455 1293 1034 627 325 217 BanII G_rGCy'C Cuts at: 1391 4054 4112 1391 Size: 2663 58 4072 Fragments arranged by size: 4072 2663 58 BbeI G_GCGC'C Cuts at: 2825 2825 Size: 6793 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5822 BbvCI CC'TCA_GC Cuts at: 3452 3741 3452 Size: 289 6504 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2706 2740 4984 5018 1903 Size: 34 50 34 685 34 2244 34 3678 Fragments arranged by size: 3678 2244 685 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4685 5895 4685 Size: 1210 5583 BclI T'GATC_A Cuts at: 1548 1548 Size: 6793 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3033 5404 136 Size: 107 122 71 631 605 1361 2371 1525 Fragments arranged by size: 2371 1525 1361 631 605 122 107 71 BglII A'GATC_T Cuts at: 2085 3732 6788 2085 Size: 1647 3056 2090 Fragments arranged by size: 3056 2090 1647 BlpI GC'TnA_GC Cuts at: 4210 4210 Size: 6793 Bme1580I G_kGCm'C Cuts at: 1790 2538 3401 4054 4854 6010 1790 Size: 748 863 653 800 1156 2573 Fragments arranged by size: 2573 1156 863 800 748 653 BmgBI CAC'GTC Cuts at: 1318 3472 1318 Size: 2154 4639 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2173 3804 4524 Size: 416 53 83 399 24 756 1631 720 Cuts at: 4524 5482 442 Size: 958 1753 Fragments arranged by size: 1753 1631 958 756 720 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6793 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2048 2393 2492 2977 5453 1239 Size: 809 345 99 485 2476 2579 Fragments arranged by size: 2579 2476 809 485 345 99 Bpu10I CC'TnA_GC Cuts at: 3007 3452 3741 3007 Size: 445 289 6059 Fragments arranged by size: 6059 445 289 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3656 4918 5783 6081 3656 Size: 1262 865 298 4368 Fragments arranged by size: 4368 1262 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2834 3490 3800 5456 882 Size: 617 1335 656 310 1656 2219 Fragments arranged by size: 2219 1656 1335 656 617 310 BsaAI yAC'GTr Cuts at: 493 1204 2122 4584 493 Size: 711 918 2462 2702 Fragments arranged by size: 2702 2462 918 711 BsaBI GATnn'nnATC Cuts at: 2957 6787 2957 Size: 3830 2963 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2033 2822 3683 4600 Size: 53 83 186 936 500 789 861 917 Cuts at: 4600 4982 5617 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 917 861 789 635 500 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5226 5624 5898 6045 6456 5226 Size: 398 274 147 411 5563 Fragments arranged by size: 5563 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2833 2863 3809 3839 1178 Size: 30 165 30 1430 30 946 30 4132 Fragments arranged by size: 4132 1430 946 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2603 3707 3961 1186 Size: 1417 1104 254 4018 Fragments arranged by size: 4018 1417 1104 254 BseYI C'CCAG_C Cuts at: 3479 5996 6492 3479 Size: 2517 496 3780 Fragments arranged by size: 3780 2517 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2321 2635 3155 3491 3592 1163 Size: 573 585 314 520 336 101 4364 Fragments arranged by size: 4364 585 573 520 336 314 101 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2716 4243 5004 6032 1540 Size: 275 186 715 1527 761 1028 2301 Fragments arranged by size: 2301 1527 1028 761 715 275 186 BsiHKAI G_wGCw'C Cuts at: 2481 3605 4854 4939 6010 2481 Size: 1124 1249 85 1071 3264 Fragments arranged by size: 3264 1249 1124 1071 85 BsmI GAATG_Cn' Cuts at: 2707 4304 4397 2707 Size: 1597 93 5103 Fragments arranged by size: 5103 1597 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2024 2040 3674 3949 2024 Size: 16 1634 275 4868 Fragments arranged by size: 4868 1634 275 16 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2481 2538 3401 3605 4054 4112 4854 Size: 399 691 57 863 204 449 58 742 Cuts at: 4854 4939 6010 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 863 742 691 449 399 204 85 58 57 BspEI T'CCGG_A Cuts at: 6456 6456 Size: 6793 BspHI T'CATG_A Cuts at: 4682 6412 4682 Size: 1730 5063 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2521 3456 4148 844 Size: 1677 935 692 3489 Fragments arranged by size: 3489 1677 935 692 BsrBI CCG'CTC Cuts at: 1394 1823 4680 1394 Size: 429 2857 3507 Fragments arranged by size: 3507 2857 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2104 5288 5462 65 Size: 1183 856 3184 174 1396 Fragments arranged by size: 3184 1396 1183 856 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3685 5437 5624 1700 Size: 110 1875 1752 187 2869 Fragments arranged by size: 2869 1875 1752 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6793 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6793 BssSI C'ACGA_G Cuts at: 3920 4853 5865 3920 Size: 933 1012 4848 Fragments arranged by size: 4848 1012 933 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6793 BstBI TT'CG_AA Cuts at: 2368 5597 2368 Size: 3229 3564 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6793 BstXI CCAn_nnnn'nTGG Cuts at: 2946 2946 Size: 6793 BstYI r'GATC_y Cuts at: 1972 2085 2622 2934 3162 3217 3426 3732 3756 Size: 113 537 312 228 55 209 306 24 Cuts at: 3756 4123 4490 4875 4892 6344 6430 6442 6788 Size: 367 367 385 17 1452 86 12 346 Cuts at: 6788 1972 Size: 1977 Fragments arranged by size: 1977 1452 537 385 367 367 346 312 306 228 209 113 86 55 24 17 12 Bsu36I CC'TnA_GG Cuts at: 3278 3932 3278 Size: 654 6139 BtgI C'CryG_G Cuts at: 513 1065 3583 3906 513 Size: 552 2518 323 3400 Fragments arranged by size: 3400 2518 552 323 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3220 487 Size: 683 56 1994 4060 Fragments arranged by size: 4060 1994 683 56 BtsI GCAGTG_nn' Cuts at: 2163 3635 4309 5103 5123 2163 Size: 1472 674 794 20 3833 Fragments arranged by size: 3833 1472 794 674 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6793 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6758 DraI TTT'AAA Cuts at: 2487 3069 4097 2487 Size: 582 1028 5183 Fragments arranged by size: 5183 1028 582 DraIII CAC_nnn'GTG Cuts at: 3190 4060 3190 Size: 870 5923 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5800 809 Size: 507 4484 1802 Fragments arranged by size: 4484 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2713 4240 5129 Size: 54 1425 50 275 18 883 1527 889 Cuts at: 5129 6758 8 Size: 1629 43 Fragments arranged by size: 1629 1527 1425 889 883 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2713 4240 1537 Size: 275 901 1527 4090 Fragments arranged by size: 4090 1527 901 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2832 4018 4723 1353 Size: 1479 1186 705 3423 Fragments arranged by size: 3423 1479 1186 705 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3818 3886 5380 5754 5900 387 Size: 1064 138 2229 68 1494 374 146 1280 Fragments arranged by size: 2229 1494 1280 1064 374 146 138 68 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2393 2492 2977 4856 5453 6240 Size: 393 416 345 99 485 1879 597 787 Cuts at: 6240 6508 1239 Size: 268 1524 Fragments arranged by size: 1879 1524 787 597 485 416 393 345 268 99 EcoNI CCTnn'n_nnAGG Cuts at: 2630 2630 Size: 6793 EcoO109I rG'GnC_Cy Cuts at: 2113 2448 3456 4050 2113 Size: 335 1008 594 4856 Fragments arranged by size: 4856 1008 594 335 EcoRI G'AATT_C Cuts at: 4102 4480 4102 Size: 378 6415 FspI TGC'GCA Cuts at: 5299 5299 Size: 6793 HaeII r_GCGC'y Cuts at: 1678 2825 5940 1678 Size: 1147 3115 2531 Fragments arranged by size: 3115 2531 1147 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2651 2683 2833 2865 3302 Size: 32 135 32 1079 32 150 32 437 Cuts at: 3302 3334 3724 3756 3953 3985 4058 4090 5434 Size: 32 390 32 197 32 73 32 1344 Cuts at: 5434 5466 5508 5540 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1344 1079 437 390 197 150 135 73 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3558 3771 4137 4383 669 Size: 988 281 1620 213 366 246 3079 Fragments arranged by size: 3079 1620 988 366 281 246 213 HindIII A'AGCT_T Cuts at: 748 748 Size: 6793 HpaI GTT'AAC Cuts at: 3558 3771 4383 3558 Size: 213 612 5968 Fragments arranged by size: 5968 612 213 KasI G'GCGC_C Cuts at: 2821 2821 Size: 6793 KpnI G_GTAC'C Cuts at: 4118 4118 Size: 6793 MfeI C'AATT_G Cuts at: 3612 4392 3612 Size: 780 6013 MluI A'CGCG_T Cuts at: 6669 6669 Size: 6793 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3195 3846 5907 6091 1305 Size: 1890 651 2061 184 2007 Fragments arranged by size: 2061 2007 1890 651 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4971 Fragments arranged by size: 4971 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3614 3857 3908 3920 4751 5110 5269 Size: 648 2448 243 51 12 831 359 159 Cuts at: 5269 518 Size: 2042 Fragments arranged by size: 2448 2042 831 648 359 243 159 51 12 MspA1I CmG'CkG Cuts at: 1606 2051 2323 3042 3745 4209 4886 6034 6279 Size: 445 272 719 703 464 677 1148 245 Cuts at: 6279 1606 Size: 2120 Fragments arranged by size: 2120 1148 719 703 677 464 445 272 245 NarI GG'CG_CC Cuts at: 2822 2822 Size: 6793 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6241 NdeI CA'TA_TG Cuts at: 387 387 Size: 6793 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6793 NotI GC'GGCC_GC Cuts at: 4240 4240 Size: 6793 NspI r_CATG'y Cuts at: 1132 1229 3919 4151 5696 1132 Size: 97 2690 232 1545 2229 Fragments arranged by size: 2690 2229 1545 232 97 PasI CC'CwG_GG Cuts at: 1348 3029 3056 3594 1348 Size: 1681 27 538 4547 Fragments arranged by size: 4547 1681 538 27 PciI A'CATG_T Cuts at: 5692 5692 Size: 6793 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6793 PfoI T'CCnGG_A Cuts at: 2454 2851 2993 2454 Size: 397 142 6254 Fragments arranged by size: 6254 397 142 PmeI GTTT'AAAC Cuts at: 4097 4097 Size: 6793 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4862 4894 6375 6407 4862 Size: 32 1481 32 5248 Fragments arranged by size: 5248 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2113 3456 2113 Size: 1343 5450 PshAI GACnn'nnGTC Cuts at: 1613 2784 1613 Size: 1171 5622 PsiI TTA'TAA Cuts at: 2250 4363 2250 Size: 2113 4680 PspOMI G'GGCC_C Cuts at: 4050 4050 Size: 6793 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3353 3385 3353 Size: 32 6761 PstI C_TGCA'G Cuts at: 830 2157 2322 2748 3041 4145 830 Size: 1327 165 426 293 1104 3478 Fragments arranged by size: 3478 1327 1104 426 293 165 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6793 PvuII CAG'CTG Cuts at: 2323 3042 3745 2323 Size: 719 703 5371 Fragments arranged by size: 5371 719 703 SalI G'TCGA_C Cuts at: 1936 4135 1936 Size: 2199 4594 SbfI CC_TGCA'GG Cuts at: 4145 4145 Size: 6793 ScaI AGT'ACT Cuts at: 5041 6753 5041 Size: 1712 5081 SfcI C'TryA_G Cuts at: 826 1045 2153 2318 2744 3037 3243 3995 4141 Size: 219 1108 165 426 293 206 752 146 Cuts at: 4141 5276 5957 6148 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1108 752 681 426 293 219 206 191 165 146 SfoI GGC'GCC Cuts at: 2823 2823 Size: 6793 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6793 SgrAI Cr'CCGG_yG Cuts at: 1700 3685 1700 Size: 1985 4808 SmaI CCC'GGG Cuts at: 1872 2445 3323 3396 3870 4120 1872 Size: 573 878 73 474 250 4545 Fragments arranged by size: 4545 878 573 474 250 73 SmlI C'TyrA_G Cuts at: 820 1017 1943 3671 4897 5798 6060 820 Size: 197 926 1728 1226 901 262 1553 Fragments arranged by size: 1728 1553 1226 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4584 493 Size: 4091 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6793 SphI G_CATG'C Cuts at: 1132 3919 4151 1132 Size: 2787 232 3774 Fragments arranged by size: 3774 2787 232 SspI AAT'ATT Cuts at: 5 52 4717 5 Size: 47 4665 2081 Fragments arranged by size: 4665 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6793 StyI C'CwwG_G Cuts at: 513 1065 2642 4232 513 Size: 552 1577 1590 3074 Fragments arranged by size: 3074 1590 1577 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2558 4820 4837 4990 5175 5620 325 Size: 1255 978 2262 17 153 185 445 1498 Fragments arranged by size: 2262 1498 1255 978 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3369 5039 6751 Size: 274 80 33 51 1443 1392 1670 1712 Cuts at: 6751 96 Size: 138 Fragments arranged by size: 1712 1670 1443 1392 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3266 3298 3809 3841 6753 6785 3266 Size: 32 511 32 2912 32 3274 Fragments arranged by size: 3274 2912 511 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6793 XbaI T'CTAG_A Cuts at: 4129 4129 Size: 6793 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3859 3859 Size: 6793 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6793 XmaI C'CCGG_G Cuts at: 1870 2443 3321 3394 3868 4118 1870 Size: 573 878 73 474 250 4545 Fragments arranged by size: 4545 878 573 474 250 73 XmnI GAAnn'nnTTC Cuts at: 2295 2691 4922 2295 Size: 396 2231 4166 Fragments arranged by size: 4166 2231 396 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2034 4601 276 Size: 53 83 186 936 500 2567 2468 Fragments arranged by size: 2567 2468 936 500 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI