(Circular) (Six-base) MAPSORT of: pFN21AA1885.seq Check: 4301 from: 1 to: 7894 pFN21AA1885 7894 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2218 4030 4726 2218 Size: 1812 696 5386 Fragments arranged by size: 5386 1812 696 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4464 5704 278 Size: 53 83 186 936 2928 1240 2468 Fragments arranged by size: 2928 2468 1240 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4464 4950 5237 1937 Size: 2527 486 287 4594 Fragments arranged by size: 4594 2527 486 287 Acc65I G'GTAC_C Cuts at: 2343 5215 2343 Size: 2872 5022 AclI AA'CG_TT Cuts at: 1563 6021 6394 1563 Size: 4458 373 3063 Fragments arranged by size: 4458 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2799 3452 3913 4608 5957 7341 7609 1632 Size: 1167 653 461 695 1349 1384 268 1917 Fragments arranged by size: 1917 1384 1349 1167 695 653 461 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7697 AflIII A'CryG_T Cuts at: 1203 3157 6793 7770 1203 Size: 1954 3636 977 1327 Fragments arranged by size: 3636 1954 1327 977 AgeI A'CCGG_T Cuts at: 3306 6725 3306 Size: 3419 4475 AhdI GACnn_n'nnGTC Cuts at: 6623 6623 Size: 7894 AleI CACnn'nnGTG Cuts at: 1166 2936 1166 Size: 1770 6124 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7894 AlwNI CAG_nnn'CTG Cuts at: 2771 3674 4045 4214 4528 4606 4668 4811 4969 Size: 903 371 169 314 78 62 143 158 Cuts at: 4969 5155 5637 7209 2771 Size: 186 482 1572 3456 Fragments arranged by size: 3456 1572 903 482 371 314 186 169 158 143 78 62 ApaI G_GGCC'C Cuts at: 2301 3223 3472 3502 4518 4921 2301 Size: 922 249 30 1016 403 5274 Fragments arranged by size: 5274 1016 922 403 249 30 ApaLI G'TGCA_C Cuts at: 5951 7107 5951 Size: 1156 6738 ApoI r'AATT_y Cuts at: 1493 4069 4292 5203 5429 5581 7690 1493 Size: 2576 223 911 226 152 2109 1697 Fragments arranged by size: 2576 2109 1697 911 226 223 152 AseI AT'TA_AT Cuts at: 160 1032 6448 160 Size: 872 5416 1606 Fragments arranged by size: 5416 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7894 AvaI C'yCGr_G Cuts at: 1870 1943 3318 3471 4401 4716 4785 5219 1870 Size: 73 1375 153 930 315 69 434 4545 Fragments arranged by size: 4545 1375 930 434 315 153 73 69 BamHI G'GATC_C Cuts at: 3739 4131 4347 5224 5591 3739 Size: 392 216 877 367 6042 Fragments arranged by size: 6042 877 392 367 216 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2343 2511 2714 4686 4853 Size: 325 217 627 556 168 203 1972 167 Cuts at: 4853 5215 6670 618 Size: 362 1455 1842 Fragments arranged by size: 1972 1842 1455 627 556 362 325 217 203 168 167 BanII G_rGCy'C Cuts at: 1391 2106 2301 2710 3223 3239 3472 3502 3510 Size: 715 195 409 513 16 233 30 8 Cuts at: 3510 4410 4518 4596 4750 4805 4848 4921 5186 Size: 900 108 78 154 55 43 73 265 Cuts at: 5186 5193 5213 1391 Size: 7 20 4072 Fragments arranged by size: 4072 900 715 513 409 265 233 195 154 108 78 73 55 43 30 20 16 8 7 BbeI G_GCGC'C Cuts at: 4690 4690 Size: 7894 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3293 928 Size: 971 1394 5529 Fragments arranged by size: 5529 1394 971 BbvCI CC'TCA_GC Cuts at: 2051 3675 4311 2051 Size: 1624 636 5634 Fragments arranged by size: 5634 1624 636 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4221 4255 6085 6119 1903 Size: 34 50 34 2200 34 1830 34 3678 Fragments arranged by size: 3678 2200 1830 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2441 5786 6996 2441 Size: 3345 1210 3339 Fragments arranged by size: 3345 3339 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7894 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4097 4615 6505 Size: 107 122 71 631 605 2425 518 1890 Cuts at: 6505 136 Size: 1525 Fragments arranged by size: 2425 1890 1525 631 605 518 122 107 71 BglII A'GATC_T Cuts at: 2688 4248 7889 2688 Size: 1560 3641 2693 Fragments arranged by size: 3641 2693 1560 BlpI GC'TnA_GC Cuts at: 2630 5311 2630 Size: 2681 5213 Bme1580I G_kGCm'C Cuts at: 1790 2301 3167 3223 3472 3502 4518 4856 4921 Size: 511 866 56 249 30 1016 338 65 Cuts at: 4921 5955 7111 1790 Size: 1034 1156 2573 Fragments arranged by size: 2573 1156 1034 1016 866 511 338 249 65 56 30 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7894 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2737 5625 6583 Size: 416 53 83 399 24 1320 2888 958 Cuts at: 6583 442 Size: 1753 Fragments arranged by size: 2888 1753 1320 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7894 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2198 2251 3054 3171 3353 3524 3752 4490 Size: 959 53 803 117 182 171 228 738 Cuts at: 4490 4721 5090 5132 5154 6554 1239 Size: 231 369 42 22 1400 2579 Fragments arranged by size: 2579 1400 959 803 738 369 231 228 182 171 117 53 42 22 Bpu10I CC'TnA_GC Cuts at: 2051 3675 4311 4352 4742 2051 Size: 1624 636 41 390 5203 Fragments arranged by size: 5203 1624 636 390 41 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3253 4929 6019 6884 7182 3253 Size: 1676 1090 865 298 3965 Fragments arranged by size: 3965 1676 1090 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2408 3803 6557 882 Size: 617 909 1395 2754 2219 Fragments arranged by size: 2754 2219 1395 909 617 BsaAI yAC'GTr Cuts at: 493 1204 4761 5685 493 Size: 711 3557 924 2702 Fragments arranged by size: 3557 2702 924 711 BsaBI GATnn'nnATC Cuts at: 7888 7888 Size: 7894 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4461 4687 5701 6083 Size: 53 83 186 936 2928 226 1014 382 Cuts at: 6083 6718 275 Size: 635 1451 Fragments arranged by size: 2928 1451 1014 936 635 382 226 186 83 53 BsaWI w'CCGG_w Cuts at: 2514 2817 3306 6327 6725 6999 7146 7557 2514 Size: 303 489 3021 398 274 147 411 2851 Fragments arranged by size: 3021 2851 489 411 398 303 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2052 2082 2119 2149 2189 Size: 30 165 30 649 30 37 30 40 Cuts at: 2189 2219 1178 Size: 30 6853 Fragments arranged by size: 6853 649 165 40 37 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2632 2752 4318 4328 1186 Size: 1446 120 1566 10 4752 Fragments arranged by size: 4752 1566 1446 120 10 BseYI C'CCAG_C Cuts at: 3906 4637 4661 4914 7097 7593 3906 Size: 731 24 253 2183 496 4207 Fragments arranged by size: 4207 2183 731 496 253 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2444 4415 4754 1163 Size: 573 708 1971 339 4303 Fragments arranged by size: 4303 1971 708 573 339 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5344 6105 7133 1540 Size: 275 186 3343 761 1028 2301 Fragments arranged by size: 3343 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2710 3510 4410 4596 4683 4805 5193 5955 6040 Size: 800 900 186 87 122 388 762 85 Cuts at: 6040 7111 2710 Size: 1071 3493 Fragments arranged by size: 3493 1071 900 800 762 388 186 122 87 85 BsmI GAATG_Cn' Cuts at: 4261 5405 5498 4261 Size: 1144 93 6657 Fragments arranged by size: 6657 1144 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3017 3017 Size: 7894 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2301 2710 3167 3223 3239 3472 Size: 399 316 195 409 457 56 16 233 Cuts at: 3472 3502 3510 4410 4518 4596 4683 4750 4805 Size: 30 8 900 108 78 87 67 55 Cuts at: 4805 4848 4856 4921 5186 5193 5213 5955 6040 Size: 43 8 65 265 7 20 742 85 Cuts at: 6040 7111 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 900 742 457 409 399 316 265 233 195 108 87 85 78 67 65 56 55 43 30 20 16 8 8 7 BspEI T'CCGG_A Cuts at: 7557 7557 Size: 7894 BspHI T'CATG_A Cuts at: 5783 7513 5783 Size: 1730 6164 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2218 3435 4030 4726 5249 844 Size: 1374 1217 595 696 523 3489 Fragments arranged by size: 3489 1374 1217 696 595 523 BsrBI CCG'CTC Cuts at: 1394 1823 4387 4705 5781 1394 Size: 429 2564 318 1076 3507 Fragments arranged by size: 3507 2564 1076 429 318 BsrDI GCAATG_nn' Cuts at: 65 1248 3249 6389 6563 65 Size: 1183 2001 3140 174 1396 Fragments arranged by size: 3140 2001 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2886 3306 6538 6725 1700 Size: 110 1076 420 3232 187 2869 Fragments arranged by size: 3232 2869 1076 420 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7894 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7894 BssSI C'ACGA_G Cuts at: 2501 5954 6966 2501 Size: 3453 1012 3429 Fragments arranged by size: 3453 3429 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2518 2567 4045 4606 794 Size: 1724 49 1478 561 4082 Fragments arranged by size: 4082 1724 1478 561 49 BstBI TT'CG_AA Cuts at: 6698 6698 Size: 7894 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7894 BstXI CCAn_nnnn'nTGG Cuts at: 2043 3487 2043 Size: 1444 6450 BstYI r'GATC_y Cuts at: 1972 2111 2688 3739 4131 4248 4347 4983 5224 Size: 139 577 1051 392 117 99 636 241 Cuts at: 5224 5591 5976 5993 7445 7531 7543 7889 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1051 636 577 392 385 367 346 241 139 117 99 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2238 2648 4521 2238 Size: 410 1873 5611 Fragments arranged by size: 5611 1873 410 BtgI C'CryG_G Cuts at: 513 1065 2107 2348 2748 2973 3381 3951 4177 Size: 552 1042 241 400 225 408 570 226 Cuts at: 4177 513 Size: 4230 Fragments arranged by size: 4230 1042 570 552 408 400 241 226 225 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7155 Fragments arranged by size: 7155 683 56 BtsI GCAGTG_nn' Cuts at: 2669 3180 5410 6204 6224 2669 Size: 511 2230 794 20 4339 Fragments arranged by size: 4339 2230 794 511 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7894 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2519 2554 529 Size: 35 1955 35 5869 Fragments arranged by size: 5869 1955 35 35 DraI TTT'AAA Cuts at: 5198 5198 Size: 7894 DraIII CAC_nnn'GTG Cuts at: 4934 4934 Size: 7894 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3099 3569 6901 809 Size: 507 1783 470 3332 1802 Fragments arranged by size: 3332 1802 1783 507 470 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2467 2484 2899 Size: 54 1425 50 275 18 637 17 415 Cuts at: 2899 3048 4089 5341 6230 7859 8 Size: 149 1041 1252 889 1629 43 Fragments arranged by size: 1629 1425 1252 1041 889 637 415 275 149 54 50 43 18 17 EagI C'GGCC_G Cuts at: 1537 1812 5341 1537 Size: 275 3529 4090 Fragments arranged by size: 4090 3529 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3129 3636 4266 4584 4832 5824 1353 Size: 1776 507 630 318 248 992 3423 Fragments arranged by size: 3423 1776 992 630 507 318 248 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4544 6481 6855 7001 387 Size: 1064 138 2955 1937 374 146 1280 Fragments arranged by size: 2955 1937 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2708 3508 4408 4594 4803 5191 2708 Size: 800 900 186 209 388 5411 Fragments arranged by size: 5411 900 800 388 209 186 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2198 2251 2799 3054 3171 3353 3452 Size: 393 566 53 548 255 117 182 99 Cuts at: 3452 3524 3752 3913 4490 4608 4721 5090 5132 Size: 72 228 161 577 118 113 369 42 Cuts at: 5132 5154 5957 6554 7341 7609 1239 Size: 22 803 597 787 268 1524 Fragments arranged by size: 1524 803 787 597 577 566 548 393 369 268 255 228 182 161 118 117 113 99 72 53 42 22 EcoNI CCTnn'n_nnAGG Cuts at: 2647 2651 4829 2647 Size: 4 2178 5712 Fragments arranged by size: 5712 2178 4 EcoO109I rG'GnC_Cy Cuts at: 2065 2298 2542 3219 3352 3364 3468 3498 3499 Size: 233 244 677 133 12 104 30 1 Cuts at: 3499 3903 4514 4515 4525 4822 4966 5052 2065 Size: 404 611 1 10 297 144 86 4907 Fragments arranged by size: 4907 677 611 404 297 244 233 144 133 104 86 30 12 10 1 1 EcoRI G'AATT_C Cuts at: 5203 5581 5203 Size: 378 7516 FspI TGC'GCA Cuts at: 3633 6400 3633 Size: 2767 5127 HaeII r_GCGC'y Cuts at: 1678 2155 4668 4690 4711 7041 1678 Size: 477 2513 22 21 2330 2531 Fragments arranged by size: 2531 2513 2330 477 22 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2103 2135 2189 2221 2744 Size: 32 135 32 531 32 54 32 523 Cuts at: 2744 2776 2847 2879 3175 3207 4005 4037 4152 Size: 32 71 32 296 32 798 32 115 Cuts at: 4152 4184 4304 4336 6535 6567 6609 6641 1373 Size: 32 120 32 2199 32 42 32 2626 Fragments arranged by size: 2626 2199 798 531 523 296 135 120 115 71 54 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2836 5238 5484 669 Size: 988 281 898 2402 246 3079 Fragments arranged by size: 3079 2402 988 898 281 246 HindIII A'AGCT_T Cuts at: 748 2781 5093 748 Size: 2033 2312 3549 Fragments arranged by size: 3549 2312 2033 HpaI GTT'AAC Cuts at: 5484 5484 Size: 7894 KasI G'GCGC_C Cuts at: 4686 4686 Size: 7894 KpnI G_GTAC'C Cuts at: 2347 5219 2347 Size: 2872 5022 MfeI C'AATT_G Cuts at: 5493 5493 Size: 7894 MluI A'CGCG_T Cuts at: 7770 7770 Size: 7894 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2079 7008 7192 1305 Size: 774 4929 184 2007 Fragments arranged by size: 4929 2007 774 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2469 2486 2901 4091 10 Size: 54 1425 343 637 17 415 1190 3813 Fragments arranged by size: 3813 1425 1190 637 415 343 54 17 MslI CAynn'nnrTG Cuts at: 518 1166 2876 2936 3893 5852 6211 6370 518 Size: 648 1710 60 957 1959 359 159 2042 Fragments arranged by size: 2042 1959 1710 957 648 359 159 60 MspA1I CmG'CkG Cuts at: 1606 2701 3453 3583 3695 3922 5110 5155 5310 Size: 1095 752 130 112 227 1188 45 155 Cuts at: 5310 5987 7135 7380 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1188 1148 1095 752 677 245 227 155 130 112 45 NarI GG'CG_CC Cuts at: 4687 4687 Size: 7894 NcoI C'CATG_G Cuts at: 513 1065 2107 2748 3381 3951 4177 513 Size: 552 1042 641 633 570 226 4230 Fragments arranged by size: 4230 1042 641 633 570 552 226 NdeI CA'TA_TG Cuts at: 387 387 Size: 7894 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7894 NotI GC'GGCC_GC Cuts at: 5341 5341 Size: 7894 NruI TCG'CGA Cuts at: 3070 3070 Size: 7894 NspI r_CATG'y Cuts at: 1132 1229 3161 5252 6797 1132 Size: 97 1932 2091 1545 2229 Fragments arranged by size: 2229 2091 1932 1545 97 PasI CC'CwG_GG Cuts at: 1348 3793 3991 4510 4849 4858 5177 1348 Size: 2445 198 519 339 9 319 4065 Fragments arranged by size: 4065 2445 519 339 319 198 9 PciI A'CATG_T Cuts at: 3157 6793 3157 Size: 3636 4258 PflMI CCAn_nnn'nTGG Cuts at: 1108 3382 3463 1108 Size: 2274 81 5539 Fragments arranged by size: 5539 2274 81 PfoI T'CCnGG_A Cuts at: 2123 2123 Size: 7894 PmeI GTTT'AAAC Cuts at: 5198 5198 Size: 7894 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5963 5995 7476 7508 5963 Size: 32 1481 32 6349 Fragments arranged by size: 6349 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2542 3903 4525 5052 2542 Size: 1361 622 527 5384 Fragments arranged by size: 5384 1361 622 527 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7894 PsiI TTA'TAA Cuts at: 5464 5464 Size: 7894 PspOMI G'GGCC_C Cuts at: 2297 3219 3468 3498 4514 4917 2297 Size: 922 249 30 1016 403 5274 Fragments arranged by size: 5274 1016 922 403 249 30 PstI C_TGCA'G Cuts at: 830 2215 3297 3401 4050 4084 4105 4673 4800 Size: 1385 1082 104 649 34 21 568 127 Cuts at: 4800 4965 5246 830 Size: 165 281 3478 Fragments arranged by size: 3478 1385 1082 649 568 281 165 127 104 34 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7894 PvuII CAG'CTG Cuts at: 2701 3583 3922 5110 5155 2701 Size: 882 339 1188 45 5440 Fragments arranged by size: 5440 1188 882 339 45 SacI G_AGCT'C Cuts at: 2710 3510 4410 4596 4805 5193 2710 Size: 800 900 186 209 388 5411 Fragments arranged by size: 5411 900 800 388 209 186 SalI G'TCGA_C Cuts at: 1936 5236 1936 Size: 3300 4594 SanDI GG'GwC_CC Cuts at: 2542 3903 2542 Size: 1361 6533 SapI GCTCTTCn'nnn_ Cuts at: 4266 4584 4266 Size: 318 7576 SbfI CC_TGCA'GG Cuts at: 5246 5246 Size: 7894 ScaI AGT'ACT Cuts at: 6142 7854 6142 Size: 1712 6182 SexAI A'CCwGG_T Cuts at: 3612 3612 Size: 7894 SfcI C'TryA_G Cuts at: 826 1045 2211 2406 3293 3397 4046 4080 4101 Size: 219 1166 195 887 104 649 34 21 Cuts at: 4101 4669 4796 4961 5242 6377 7058 7249 826 Size: 568 127 165 281 1135 681 191 1471 Fragments arranged by size: 1471 1166 1135 887 681 649 568 281 219 195 191 165 127 104 34 21 SfoI GGC'GCC Cuts at: 4688 4688 Size: 7894 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7894 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7894 SmaI CCC'GGG Cuts at: 1872 3320 4718 5221 1872 Size: 1448 1398 503 4545 Fragments arranged by size: 4545 1448 1398 503 SmlI C'TyrA_G Cuts at: 820 1017 1943 3232 4944 5998 6899 7161 820 Size: 197 926 1289 1712 1054 901 262 1553 Fragments arranged by size: 1712 1553 1289 1054 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5685 493 Size: 5192 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7894 SphI G_CATG'C Cuts at: 1132 5252 1132 Size: 4120 3774 SspI AAT'ATT Cuts at: 5 52 5818 5 Size: 47 5766 2081 Fragments arranged by size: 5766 2081 47 StuI AGG'CCT Cuts at: 1517 2228 2236 2642 4618 4835 1517 Size: 711 8 406 1976 217 4576 Fragments arranged by size: 4576 1976 711 406 217 8 StyI C'CwwG_G Cuts at: 513 1065 2107 2748 3381 3480 3951 4177 5333 Size: 552 1042 641 633 99 471 226 1156 Cuts at: 5333 513 Size: 3074 Fragments arranged by size: 3074 1156 1042 641 633 552 471 226 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2702 3643 3868 5921 5938 6091 6276 Size: 1255 1122 941 225 2053 17 153 185 Cuts at: 6276 6721 325 Size: 445 1498 Fragments arranged by size: 2053 1498 1255 1122 941 445 225 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6140 7852 96 Size: 274 80 33 51 1443 4163 1712 138 Fragments arranged by size: 4163 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2052 2084 2119 2151 2723 2755 3533 3565 7854 Size: 32 35 32 572 32 778 32 4289 Cuts at: 7854 7886 2052 Size: 32 2060 Fragments arranged by size: 4289 2060 778 572 35 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7894 XbaI T'CTAG_A Cuts at: 5230 5230 Size: 7894 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7894 XmaI C'CCGG_G Cuts at: 1870 3318 4716 5219 1870 Size: 1448 1398 503 4545 Fragments arranged by size: 4545 1448 1398 503 XmnI GAAnn'nnTTC Cuts at: 6023 6023 Size: 7894 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4462 5702 276 Size: 53 83 186 936 2928 1240 2468 Fragments arranged by size: 2928 2468 1240 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NsiI PacI PmlI PspXI PsrI RsrII SacII SfiI SrfI SwaI XcmI