(Circular) (Six-base) MAPSORT of: pFN21AA1880.seq Check: 6812 from: 1 to: 6541 pFN21AA1880 6541 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2118 4351 278 Size: 53 83 186 936 582 2233 2468 Fragments arranged by size: 2468 2233 936 582 186 83 53 AccI GT'mk_AC Cuts at: 1937 3884 1937 Size: 1947 4594 Acc65I G'GTAC_C Cuts at: 3862 3862 Size: 6541 AclI AA'CG_TT Cuts at: 1563 2140 4668 5041 1563 Size: 577 2528 373 3063 Fragments arranged by size: 3063 2528 577 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2247 2712 4604 5988 6256 1632 Size: 615 465 1892 1384 268 1917 Fragments arranged by size: 1917 1892 1384 615 465 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6344 AflIII A'CryG_T Cuts at: 1203 3389 5440 6417 1203 Size: 2186 2051 977 1327 Fragments arranged by size: 2186 2051 1327 977 AgeI A'CCGG_T Cuts at: 5372 5372 Size: 6541 AhdI GACnn_n'nnGTC Cuts at: 5270 5270 Size: 6541 AleI CACnn'nnGTG Cuts at: 1166 2123 1166 Size: 957 5584 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2191 1184 Size: 1007 5534 AlwNI CAG_nnn'CTG Cuts at: 4284 5856 4284 Size: 1572 4969 ApaLI G'TGCA_C Cuts at: 2060 4598 5754 2060 Size: 2538 1156 2847 Fragments arranged by size: 2847 2538 1156 ApoI r'AATT_y Cuts at: 1493 2235 3850 4076 4228 6337 1493 Size: 742 1615 226 152 2109 1697 Fragments arranged by size: 2109 1697 1615 742 226 152 AseI AT'TA_AT Cuts at: 160 1032 5095 160 Size: 872 4063 1606 Fragments arranged by size: 4063 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6541 AvaI C'yCGr_G Cuts at: 1870 1943 3866 1870 Size: 73 1923 4545 Fragments arranged by size: 4545 1923 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3005 3038 3005 Size: 33 6508 BamHI G'GATC_C Cuts at: 3871 4238 3871 Size: 367 6174 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2084 2175 2937 3772 3862 Size: 325 217 627 297 91 762 835 90 Cuts at: 3862 5317 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 835 762 627 325 297 217 91 90 BanII G_rGCy'C Cuts at: 1391 2857 3860 1391 Size: 1466 1003 4072 Fragments arranged by size: 4072 1466 1003 BbeI G_GCGC'C Cuts at: 3776 3776 Size: 6541 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5570 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3743 3777 4732 4766 1903 Size: 34 1806 34 955 34 3678 Fragments arranged by size: 3678 1806 955 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3006 4433 5643 3006 Size: 1427 1210 3904 Fragments arranged by size: 3904 1427 1210 BclI T'GATC_A Cuts at: 1548 2053 1548 Size: 505 6036 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2964 5152 136 Size: 107 122 71 631 605 1292 2188 1525 Fragments arranged by size: 2188 1525 1292 631 605 122 107 71 BglII A'GATC_T Cuts at: 6536 6536 Size: 6541 BlpI GC'TnA_GC Cuts at: 3958 3958 Size: 6541 Bme1580I G_kGCm'C Cuts at: 1790 2064 4602 5758 1790 Size: 274 2538 1156 2573 Fragments arranged by size: 2573 2538 1156 274 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6541 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2304 3382 4272 Size: 416 53 83 399 24 887 1078 890 Cuts at: 4272 5230 442 Size: 958 1753 Fragments arranged by size: 1753 1078 958 890 887 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6541 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2370 2831 2370 Size: 461 6080 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3781 5201 1239 Size: 2542 1420 2579 Fragments arranged by size: 2579 2542 1420 Bpu10I CC'TnA_GC Cuts at: 2511 2511 Size: 6541 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2509 3272 4666 5531 5829 2509 Size: 763 1394 865 298 3221 Fragments arranged by size: 3221 1394 865 763 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5204 882 Size: 617 3705 2219 Fragments arranged by size: 3705 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4332 493 Size: 711 3128 2702 Fragments arranged by size: 3128 2702 711 BsaBI GATnn'nnATC Cuts at: 2919 6535 2919 Size: 3616 2925 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2115 3773 4348 4730 Size: 53 83 186 936 582 1658 575 382 Cuts at: 4730 5365 275 Size: 635 1451 Fragments arranged by size: 1658 1451 936 635 582 575 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4974 5372 5646 5793 6204 4974 Size: 398 274 147 411 5311 Fragments arranged by size: 5311 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2844 2874 3803 3833 1178 Size: 30 165 30 1441 30 929 30 3886 Fragments arranged by size: 3886 1441 929 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2339 1186 Size: 1153 5388 BseYI C'CCAG_C Cuts at: 5744 6240 5744 Size: 496 6045 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2389 1163 Size: 573 653 5315 Fragments arranged by size: 5315 653 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3991 4752 5780 1540 Size: 275 186 1990 761 1028 2301 Fragments arranged by size: 2301 1990 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2064 4602 4687 5758 2064 Size: 2538 85 1071 2847 Fragments arranged by size: 2847 2538 1071 85 BsmI GAATG_Cn' Cuts at: 2214 2264 2512 4052 4145 2214 Size: 50 248 1540 93 4610 Fragments arranged by size: 4610 1540 248 93 50 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2857 3860 4602 4687 5758 1391 Size: 399 274 793 1003 742 85 1071 2174 Fragments arranged by size: 2174 1071 1003 793 742 399 274 85 BspEI T'CCGG_A Cuts at: 6204 6204 Size: 6541 BspHI T'CATG_A Cuts at: 2691 4430 6160 2691 Size: 1739 1730 3072 Fragments arranged by size: 3072 1739 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3147 3896 844 Size: 2303 749 3489 Fragments arranged by size: 3489 2303 749 BsrBI CCG'CTC Cuts at: 1394 1823 4428 1394 Size: 429 2605 3507 Fragments arranged by size: 3507 2605 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2832 5036 5210 65 Size: 1183 1584 2204 174 1396 Fragments arranged by size: 2204 1584 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3036 3740 3828 5185 5372 1700 Size: 110 1226 704 88 1357 187 2869 Fragments arranged by size: 2869 1357 1226 704 187 110 88 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6541 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6541 BssSI C'ACGA_G Cuts at: 4601 5613 4601 Size: 1012 5529 BstAPI GCAn_nnn'nTGC Cuts at: 794 2677 3293 794 Size: 1883 616 4042 Fragments arranged by size: 4042 1883 616 BstBI TT'CG_AA Cuts at: 3166 5345 3166 Size: 2179 4362 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6541 BstYI r'GATC_y Cuts at: 1972 3871 4238 4623 4640 6092 6178 6190 6536 Size: 1899 367 385 17 1452 86 12 346 Cuts at: 6536 1972 Size: 1977 Fragments arranged by size: 1977 1899 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3273 3534 513 Size: 552 2208 261 3520 Fragments arranged by size: 3520 2208 552 261 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5802 Fragments arranged by size: 5802 683 56 BtsI GCAGTG_nn' Cuts at: 2046 2253 4057 4851 4871 2046 Size: 207 1804 794 20 3716 Fragments arranged by size: 3716 1804 794 207 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6541 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6506 DraI TTT'AAA Cuts at: 2565 3845 2565 Size: 1280 5261 DraIII CAC_nnn'GTG Cuts at: 3211 3211 Size: 6541 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5548 809 Size: 507 4232 1802 Fragments arranged by size: 4232 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3738 3988 4877 Size: 54 1425 50 275 18 1908 250 889 Cuts at: 4877 6506 8 Size: 1629 43 Fragments arranged by size: 1908 1629 1425 889 275 250 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3988 1537 Size: 275 2176 4090 Fragments arranged by size: 4090 2176 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2028 4471 1353 Size: 675 2443 3423 Fragments arranged by size: 3423 2443 675 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5128 5502 5648 387 Size: 1064 138 3539 374 146 1280 Fragments arranged by size: 3539 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2247 2712 3781 4604 5201 5988 6256 Size: 393 615 465 1069 823 597 787 268 Cuts at: 6256 1239 Size: 1524 Fragments arranged by size: 1524 1069 823 787 615 597 465 393 268 EcoRI G'AATT_C Cuts at: 3850 4228 3850 Size: 378 6163 FspI TGC'GCA Cuts at: 5047 5047 Size: 6541 HaeII r_GCGC'y Cuts at: 1678 3776 5688 1678 Size: 2098 1912 2531 Fragments arranged by size: 2531 2098 1912 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2375 2407 2864 2896 3023 Size: 32 135 32 803 32 457 32 127 Cuts at: 3023 3055 5182 5214 5256 5288 1373 Size: 32 2127 32 42 32 2626 Fragments arranged by size: 2626 2127 803 457 135 127 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2475 3885 4131 669 Size: 988 281 537 1410 246 3079 Fragments arranged by size: 3079 1410 988 537 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6541 HpaI GTT'AAC Cuts at: 2475 4131 2475 Size: 1656 4885 KasI G'GCGC_C Cuts at: 3772 3772 Size: 6541 KpnI G_GTAC'C Cuts at: 3866 3866 Size: 6541 MfeI C'AATT_G Cuts at: 2943 3291 4140 2943 Size: 348 849 5344 Fragments arranged by size: 5344 849 348 MluI A'CGCG_T Cuts at: 6417 6417 Size: 6541 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3308 3834 5655 5839 1305 Size: 2003 526 1821 184 2007 Fragments arranged by size: 2007 2003 1821 526 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4719 Fragments arranged by size: 4719 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2082 2123 2361 2495 2768 3059 4499 Size: 648 916 41 238 134 273 291 1440 Cuts at: 4499 4858 5017 518 Size: 359 159 2042 Fragments arranged by size: 2042 1440 916 648 359 291 273 238 159 134 41 MspA1I CmG'CkG Cuts at: 1606 3957 4634 5782 6027 1606 Size: 2351 677 1148 245 2120 Fragments arranged by size: 2351 2120 1148 677 245 NarI GG'CG_CC Cuts at: 3773 3773 Size: 6541 NcoI C'CATG_G Cuts at: 513 1065 3273 3534 513 Size: 552 2208 261 3520 Fragments arranged by size: 3520 2208 552 261 NdeI CA'TA_TG Cuts at: 387 387 Size: 6541 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6541 NotI GC'GGCC_GC Cuts at: 3988 3988 Size: 6541 NspI r_CATG'y Cuts at: 1132 1229 2494 2815 3393 3899 5444 1132 Size: 97 1265 321 578 506 1545 2229 Fragments arranged by size: 2229 1545 1265 578 506 321 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6541 PciI A'CATG_T Cuts at: 3389 5440 3389 Size: 2051 4490 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6541 PfoI T'CCnGG_A Cuts at: 2754 2754 Size: 6541 PmeI GTTT'AAAC Cuts at: 3845 3845 Size: 6541 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4610 4642 6123 6155 4610 Size: 32 1481 32 4996 Fragments arranged by size: 4996 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2737 1613 Size: 1124 5417 PsiI TTA'TAA Cuts at: 4111 4111 Size: 6541 PstI C_TGCA'G Cuts at: 830 2041 2260 3158 3893 830 Size: 1211 219 898 735 3478 Fragments arranged by size: 3478 1211 898 735 219 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6541 SalI G'TCGA_C Cuts at: 1936 3883 1936 Size: 1947 4594 SbfI CC_TGCA'GG Cuts at: 3893 3893 Size: 6541 ScaI AGT'ACT Cuts at: 4789 6501 4789 Size: 1712 4829 SfcI C'TryA_G Cuts at: 826 1045 2037 2256 3154 3490 3889 5024 5705 Size: 219 992 219 898 336 399 1135 681 Cuts at: 5705 5896 826 Size: 191 1471 Fragments arranged by size: 1471 1135 992 898 681 399 336 219 219 191 SfoI GGC'GCC Cuts at: 3774 3774 Size: 6541 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6541 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6541 SmaI CCC'GGG Cuts at: 1872 3868 1872 Size: 1996 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2524 3251 4645 5546 5808 820 Size: 197 926 581 727 1394 901 262 1553 Fragments arranged by size: 1553 1394 926 901 727 581 262 197 SnaBI TAC'GTA Cuts at: 493 4332 493 Size: 3839 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6541 SphI G_CATG'C Cuts at: 1132 2815 3899 1132 Size: 1683 1084 3774 Fragments arranged by size: 3774 1683 1084 SspI AAT'ATT Cuts at: 5 52 2183 4465 5 Size: 47 2131 2282 2081 Fragments arranged by size: 2282 2131 2081 47 StuI AGG'CCT Cuts at: 1517 2725 1517 Size: 1208 5333 StyI C'CwwG_G Cuts at: 513 1065 3273 3534 3980 513 Size: 552 2208 261 446 3074 Fragments arranged by size: 3074 2208 552 446 261 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2955 3042 4568 4585 4738 4923 5368 Size: 1255 1375 87 1526 17 153 185 445 Cuts at: 5368 325 Size: 1498 Fragments arranged by size: 1526 1498 1375 1255 445 185 153 87 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4787 6499 96 Size: 274 80 33 51 1443 2810 1712 138 Fragments arranged by size: 2810 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6501 6533 6501 Size: 32 6509 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6541 XbaI T'CTAG_A Cuts at: 3877 3877 Size: 6541 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3404 3404 Size: 6541 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6541 XmaI C'CCGG_G Cuts at: 1870 3866 1870 Size: 1996 4545 XmnI GAAnn'nnTTC Cuts at: 4670 4670 Size: 6541 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2116 4349 276 Size: 53 83 186 936 582 2233 2468 Fragments arranged by size: 2468 2233 936 582 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI