Restriction map for
pFN21AA1868
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII AccI AccII AccIII AclI
AflII AgeI AocI Aor51HI AscI
AseI AsnI AspI AvaI AviII
AvrII BamHI BbrPI BbvCI BcgI
BciVI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspMI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoT22I FseI
FspI HincII HindII HpaI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspI SwaI
Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 1985 GGTACC G^GTACC
AcyI 1334 GGCGCC G[GA]^CG[CT]C
AflIII 1481 ACATGT A^C[GA][CT]GT
AhdI 1405 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 475 GTGCAC G^TGCAC
ApaI 1310 GGGCCC GGGCC^C
ApaLI 475 GTGCAC G^TGCAC
Asp718I 1985 GGTACC G^GTACC
AspEI 1405 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1334 GGCGCC GGCGC^C
BclI 779 TGATCA T^GATCA
BcnI 1966 CCCGG CC^[GC]GG
BsaHI 1334 GGCGCC G[GA]^CG[CT]C
BsaWI 345 ACCGGA [AT]^CCGG[AT]
BsmI 100 GAATGC GAATGC(1/-1)
Bsp1407I 1577 TGTACA T^GTACA
BspLU11I 1481 ACATGT A^CATGT
BsrBI 1932 GAGCGG CCGCTC(-3/-3)
BsrGI 1577 TGTACA T^GTACA
BstAPI 1011 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 464 CCAACCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 487 TTTAAA TTT^AAA
DsaI 1862 CCACGG C^C[GA][CT]GG
Eam1105I 1405 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1282 GATATC GAT^ATC
FbaI 779 TGATCA T^GATCA
Hin1I 1334 GGCGCC G[GA]^CG[CT]C
KasI 1334 GGCGCC G^GCGCC
KpnI 1985 GGTACC GGTAC^C
MslI 1233 CACAAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1334 GGCGCC GG^CGCC
NciI 1966 CCCGG CC^[GC]GG
NspI 1481 ACATGT [GA]CATG^[CT]
PpuMI 1397 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 646 CAGCTG CAG^CTG
SacI 1571 GAGCTC GAGCT^C
SapI 1717 GAAGAGC GCTCTTC(1/4)
SfoI 1334 GGCGCC GGC^GCC
SmlI 414 CTTGAG C^T[CT][GA]AG
SpeI 39 ACTAGT A^CTAGT
SspBI 1577 TGTACA T^GTACA
TaqI 224 TCGA T^CGA
TthHB8I 224 TCGA T^CGA
XbaI 753 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 20 enzymes) #
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AvaII 1398 GGACC G^G[AT]CC
1776 GGACC
BalI 1172 TGGCCA TGG^CCA
1533 TGGCCA
BglI 898 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1489 GCCAAGGTGGC
BsgI 1138 CTGCAC GTGCAG(16/14)
1924 GTGCAG
BsrI 1200 ACTGG ACTGG(1/-1)
1990 CCAGT
EcoRI 289 GAATTC G^AATTC
1690 GAATTC
FauI 1338 CCCGC CCCGC(4/6)
1934 GCGGG
HaeII 1025 GGCGCT [GA]GCGC^[CT]
1334 GGCGCC
HapII 346 CCGG C^CGG
1967 CCGG
HgaI 803 GCGTC GACGC(5/10)
1195 GCGTC
HindIII 580 AAGCTT A^AGCTT
1852 AAGCTT
HpaII 346 CCGG C^CGG
1967 CCGG
MaeII 663 ACGT A^CGT
1154 ACGT
MluNI 1172 TGGCCA TGG^CCA
1533 TGGCCA
MscI 1172 TGGCCA TGG^CCA
1533 TGGCCA
MspI 346 CCGG C^CGG
1967 CCGG
PflMI 279 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
642 CCAGCAGCTGG
ScaI 329 AGTACT AGT^ACT
1430 AGTACT
StuI 868 AGGCCT AGG^CCT
1500 AGGCCT
Van91I 279 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
642 CCAGCAGCTGG
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI BanI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BsrDI BssKI BssSI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI PstI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII XmnI