(Circular) (Six-base) MAPSORT of: pFN21AA1868.seq Check: 1484 from: 1 to: 6700 pFN21AA1868 6700 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4510 278 Size: 53 83 186 936 2974 2468 Fragments arranged by size: 2974 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4043 1937 Size: 2106 4594 Acc65I G'GTAC_C Cuts at: 3990 4021 3990 Size: 31 6669 AclI AA'CG_TT Cuts at: 1563 4827 5200 1563 Size: 3264 373 3063 Fragments arranged by size: 3264 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2987 3119 3290 3419 4763 6147 6415 1632 Size: 1355 132 171 129 1344 1384 268 1917 Fragments arranged by size: 1917 1384 1355 1344 268 171 132 129 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6503 AflIII A'CryG_T Cuts at: 1203 3486 5599 6576 1203 Size: 2283 2113 977 1327 Fragments arranged by size: 2283 2113 1327 977 AgeI A'CCGG_T Cuts at: 5531 5531 Size: 6700 AhdI GACnn_n'nnGTC Cuts at: 3415 5429 3415 Size: 2014 4686 AleI CACnn'nnGTG Cuts at: 1166 3242 1166 Size: 2076 4624 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6700 AlwNI CAG_nnn'CTG Cuts at: 2290 2653 2848 3022 3142 3256 4443 6015 2290 Size: 363 195 174 120 114 1187 1572 2975 Fragments arranged by size: 2975 1572 1187 363 195 174 120 114 ApaI G_GGCC'C Cuts at: 3319 3319 Size: 6700 ApaLI G'TGCA_C Cuts at: 2480 4757 5913 2480 Size: 2277 1156 3267 Fragments arranged by size: 3267 2277 1156 ApoI r'AATT_y Cuts at: 1493 2072 2294 3695 3715 4009 4235 4387 6496 Size: 579 222 1401 20 294 226 152 2109 Cuts at: 6496 1493 Size: 1697 Fragments arranged by size: 2109 1697 1401 579 294 226 222 152 20 AseI AT'TA_AT Cuts at: 160 1032 5254 160 Size: 872 4222 1606 Fragments arranged by size: 4222 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6700 AvaI C'yCGr_G Cuts at: 1870 1943 4025 1870 Size: 73 2082 4545 Fragments arranged by size: 4545 2082 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3548 3581 3548 Size: 33 6667 BamHI G'GATC_C Cuts at: 4030 4397 4030 Size: 367 6333 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2911 3339 3990 4021 5476 Size: 325 217 627 1124 428 651 31 1455 Cuts at: 5476 618 Size: 1842 Fragments arranged by size: 1842 1455 1124 651 627 428 325 217 31 BanII G_rGCy'C Cuts at: 1391 3319 3580 3951 4019 1391 Size: 1928 261 371 68 4072 Fragments arranged by size: 4072 1928 371 261 68 BbeI G_GCGC'C Cuts at: 3343 3343 Size: 6700 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2085 3645 3908 928 Size: 971 186 1560 263 3720 Fragments arranged by size: 3720 1560 971 263 186 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4891 4925 1903 Size: 34 2954 34 3678 Fragments arranged by size: 3678 2954 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4592 5802 4592 Size: 1210 5490 BclI T'GATC_A Cuts at: 1548 2784 1548 Size: 1236 5464 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2909 3500 5311 Size: 107 122 71 631 605 1237 591 1811 Cuts at: 5311 136 Size: 1525 Fragments arranged by size: 1811 1525 1237 631 605 591 122 107 71 BglII A'GATC_T Cuts at: 6695 6695 Size: 6700 BlpI GC'TnA_GC Cuts at: 4117 4117 Size: 6700 Bme1580I G_kGCm'C Cuts at: 1790 2484 3319 4761 5917 1790 Size: 694 835 1442 1156 2573 Fragments arranged by size: 2573 1442 1156 835 694 BmgBI CAC'GTC Cuts at: 1318 3160 1318 Size: 1842 4858 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3989 4431 5389 Size: 416 53 83 399 24 2572 442 958 Cuts at: 5389 442 Size: 1753 Fragments arranged by size: 2572 1753 958 442 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6700 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2420 2505 2786 3072 3278 3452 5360 1239 Size: 1181 85 281 286 206 174 1908 2579 Fragments arranged by size: 2579 1908 1181 286 281 206 174 85 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2440 4825 5690 5988 2440 Size: 2385 865 298 3152 Fragments arranged by size: 3152 2385 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2205 2341 2396 3059 5363 882 Size: 617 706 136 55 663 2304 2219 Fragments arranged by size: 2304 2219 706 663 617 136 55 BsaAI yAC'GTr Cuts at: 493 1204 4491 493 Size: 711 3287 2702 Fragments arranged by size: 3287 2702 711 BsaBI GATnn'nnATC Cuts at: 6694 6694 Size: 6700 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3340 4507 4889 5524 Size: 53 83 186 936 1807 1167 382 635 Cuts at: 5524 275 Size: 1451 Fragments arranged by size: 1807 1451 1167 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2350 5133 5531 5805 5952 6363 2350 Size: 2783 398 274 147 411 2687 Fragments arranged by size: 2783 2687 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6475 Fragments arranged by size: 6475 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2255 2375 3139 3290 1186 Size: 1069 120 764 151 4596 Fragments arranged by size: 4596 1069 764 151 120 BseYI C'CCAG_C Cuts at: 3831 5903 6399 3831 Size: 2072 496 4132 Fragments arranged by size: 4132 2072 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3128 3950 1163 Size: 573 1392 822 3913 Fragments arranged by size: 3913 1392 822 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4150 4911 5939 1540 Size: 275 186 2149 761 1028 2301 Fragments arranged by size: 2301 2149 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2247 2484 3516 3580 4761 4846 5917 2247 Size: 237 1032 64 1181 85 1071 3030 Fragments arranged by size: 3030 1181 1071 1032 237 85 64 BsmI GAATG_Cn' Cuts at: 2111 4211 4304 2111 Size: 2100 93 4507 Fragments arranged by size: 4507 2100 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2247 2484 3319 3516 3580 3951 4019 Size: 399 457 237 835 197 64 371 68 Cuts at: 4019 4761 4846 5917 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 835 742 457 399 371 237 197 85 68 64 BspEI T'CCGG_A Cuts at: 6363 6363 Size: 6700 BspHI T'CATG_A Cuts at: 2023 3453 3885 4589 6319 2023 Size: 1430 432 704 1730 2404 Fragments arranged by size: 2404 1730 1430 704 432 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4055 844 Size: 3211 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3939 4587 1394 Size: 429 2116 648 3507 Fragments arranged by size: 3507 2116 648 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3097 3633 3676 5195 5369 65 Size: 1183 1849 536 43 1519 174 1396 Fragments arranged by size: 1849 1519 1396 1183 536 174 43 BsrFI r'CCGG_y Cuts at: 1700 1810 5344 5531 1700 Size: 110 3534 187 2869 Fragments arranged by size: 3534 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3582 96 Size: 3486 3214 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6700 BssSI C'ACGA_G Cuts at: 2869 3151 3509 4760 5772 2869 Size: 282 358 1251 1012 3797 Fragments arranged by size: 3797 1251 1012 358 282 BstAPI GCAn_nnn'nTGC Cuts at: 794 3022 794 Size: 2228 4472 BstBI TT'CG_AA Cuts at: 5504 5504 Size: 6700 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6700 BstXI CCAn_nnnn'nTGG Cuts at: 2476 2476 Size: 6700 BstYI r'GATC_y Cuts at: 1972 2147 2185 2703 3529 4030 4397 4782 4799 Size: 175 38 518 826 501 367 385 17 Cuts at: 4799 6251 6337 6349 6695 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 826 518 501 385 367 346 175 86 38 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3867 513 Size: 552 939 1863 3346 Fragments arranged by size: 3346 1863 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3371 487 Size: 683 56 2145 3816 Fragments arranged by size: 3816 2145 683 56 BtsI GCAGTG_nn' Cuts at: 3938 4216 5010 5030 3938 Size: 278 794 20 5608 Fragments arranged by size: 5608 794 278 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6700 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6665 DraI TTT'AAA Cuts at: 2494 4004 2494 Size: 1510 5190 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5707 809 Size: 507 4391 1802 Fragments arranged by size: 4391 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3177 3538 3976 Size: 54 1425 50 275 18 1347 361 438 Cuts at: 3976 4147 5036 6665 8 Size: 171 889 1629 43 Fragments arranged by size: 1629 1425 1347 889 438 361 275 171 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4147 1537 Size: 275 2335 4090 Fragments arranged by size: 4090 2335 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2507 3717 3771 4630 1353 Size: 1154 1210 54 859 3423 Fragments arranged by size: 3423 1210 1154 859 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2160 2807 3674 3925 5287 5661 Size: 1064 138 571 647 867 251 1362 374 Cuts at: 5661 5807 387 Size: 146 1280 Fragments arranged by size: 1362 1280 1064 867 647 571 374 251 146 138 EcoICRI GAG'CTC Cuts at: 3578 3578 Size: 6700 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2420 2505 2786 2987 3072 3119 3278 Size: 393 788 85 281 201 85 47 159 Cuts at: 3278 3290 3419 3452 4763 5360 6147 6415 1239 Size: 12 129 33 1311 597 787 268 1524 Fragments arranged by size: 1524 1311 788 787 597 393 281 268 201 159 129 85 85 47 33 12 EcoO109I rG'GnC_Cy Cuts at: 3315 3316 3403 3315 Size: 1 87 6612 Fragments arranged by size: 6612 87 1 EcoRI G'AATT_C Cuts at: 2294 3695 4009 4387 2294 Size: 1401 314 378 4607 Fragments arranged by size: 4607 1401 378 314 EcoRV GAT'ATC Cuts at: 3289 3289 Size: 6700 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2242 2242 Size: 6700 FspI TGC'GCA Cuts at: 5206 5206 Size: 6700 HaeII r_GCGC'y Cuts at: 1678 3034 3343 5847 1678 Size: 1356 309 2504 2531 Fragments arranged by size: 2531 2504 1356 309 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2150 2182 2258 2290 3401 Size: 32 135 32 578 32 76 32 1111 Cuts at: 3401 3433 5341 5373 5415 5447 1373 Size: 32 1908 32 42 32 2626 Fragments arranged by size: 2626 1908 1111 578 135 76 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4044 4290 669 Size: 988 281 2106 246 3079 Fragments arranged by size: 3079 2106 988 281 246 HindIII A'AGCT_T Cuts at: 748 2585 3857 748 Size: 1837 1272 3591 Fragments arranged by size: 3591 1837 1272 HpaI GTT'AAC Cuts at: 4290 4290 Size: 6700 KasI G'GCGC_C Cuts at: 3339 3339 Size: 6700 KpnI G_GTAC'C Cuts at: 3994 4025 3994 Size: 31 6669 MfeI C'AATT_G Cuts at: 4299 4299 Size: 6700 MluI A'CGCG_T Cuts at: 6576 6576 Size: 6700 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2520 5814 5998 1305 Size: 1215 3294 184 2007 Fragments arranged by size: 3294 2007 1215 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3179 3540 10 Size: 54 1425 343 1347 361 3170 Fragments arranged by size: 3170 1425 1347 361 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3242 4658 5017 5176 518 Size: 648 2076 1416 359 159 2042 Fragments arranged by size: 2076 2042 1416 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2653 2775 3346 4116 4793 5941 6186 1606 Size: 1047 122 571 770 677 1148 245 2120 Fragments arranged by size: 2120 1148 1047 770 677 571 245 122 NarI GG'CG_CC Cuts at: 3340 3340 Size: 6700 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5209 Fragments arranged by size: 5209 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6700 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6700 NotI GC'GGCC_GC Cuts at: 4147 4147 Size: 6700 NspI r_CATG'y Cuts at: 1132 1229 3490 4058 5603 1132 Size: 97 2261 568 1545 2229 Fragments arranged by size: 2261 2229 1545 568 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6700 PciI A'CATG_T Cuts at: 3486 5599 3486 Size: 2113 4587 PflMI CCAn_nnn'nTGG Cuts at: 1108 2290 2653 1108 Size: 1182 363 5155 Fragments arranged by size: 5155 1182 363 PfoI T'CCnGG_A Cuts at: 2505 2513 3049 2505 Size: 8 536 6156 Fragments arranged by size: 6156 536 8 PmeI GTTT'AAAC Cuts at: 4004 4004 Size: 6700 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4769 4801 6282 6314 4769 Size: 32 1481 32 5155 Fragments arranged by size: 5155 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3403 3403 Size: 6700 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6700 PsiI TTA'TAA Cuts at: 4270 4270 Size: 6700 PspOMI G'GGCC_C Cuts at: 3315 3315 Size: 6700 PstI C_TGCA'G Cuts at: 830 3252 3324 3351 4052 830 Size: 2422 72 27 701 3478 Fragments arranged by size: 3478 2422 701 72 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6700 PvuII CAG'CTG Cuts at: 2653 2653 Size: 6700 SacI G_AGCT'C Cuts at: 3580 3580 Size: 6700 SalI G'TCGA_C Cuts at: 1936 4042 1936 Size: 2106 4594 SapI GCTCTTCn'nnn_ Cuts at: 3717 3717 Size: 6700 SbfI CC_TGCA'GG Cuts at: 4052 4052 Size: 6700 ScaI AGT'ACT Cuts at: 2336 3437 4948 6660 2336 Size: 1101 1511 1712 2376 Fragments arranged by size: 2376 1712 1511 1101 SfcI C'TryA_G Cuts at: 826 1045 3248 3320 3347 4048 5183 5864 6055 Size: 219 2203 72 27 701 1135 681 191 Cuts at: 6055 826 Size: 1471 Fragments arranged by size: 2203 1471 1135 701 681 219 191 72 27 SfoI GGC'GCC Cuts at: 3341 3341 Size: 6700 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6700 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6700 SmaI CCC'GGG Cuts at: 1872 4027 1872 Size: 2155 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2419 4804 5705 5967 820 Size: 197 926 476 2385 901 262 1553 Fragments arranged by size: 2385 1553 926 901 476 262 197 SnaBI TAC'GTA Cuts at: 493 4491 493 Size: 3998 2702 SpeI A'CTAG_T Cuts at: 152 2044 152 Size: 1892 4808 SphI G_CATG'C Cuts at: 1132 4058 1132 Size: 2926 3774 SspI AAT'ATT Cuts at: 5 52 4624 5 Size: 47 4572 2081 Fragments arranged by size: 4572 2081 47 StuI AGG'CCT Cuts at: 1517 2875 3507 1517 Size: 1358 632 4710 Fragments arranged by size: 4710 1358 632 StyI C'CwwG_G Cuts at: 513 1065 2004 2907 2949 3495 4139 513 Size: 552 939 903 42 546 644 3074 Fragments arranged by size: 3074 939 903 644 552 546 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4727 4744 4897 5082 5527 325 Size: 1255 3147 17 153 185 445 1498 Fragments arranged by size: 3147 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2334 3171 3435 Size: 274 80 33 51 1443 357 837 264 Cuts at: 3435 3582 4946 6658 96 Size: 147 1364 1712 138 Fragments arranged by size: 1712 1443 1364 837 357 274 264 147 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3031 3063 6660 6692 3031 Size: 32 3597 32 3039 Fragments arranged by size: 3597 3039 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6700 XbaI T'CTAG_A Cuts at: 2758 4036 2758 Size: 1278 5422 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2726 3157 3874 2726 Size: 431 717 5552 Fragments arranged by size: 5552 717 431 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6700 XmaI C'CCGG_G Cuts at: 1870 4025 1870 Size: 2155 4545 XmnI GAAnn'nnTTC Cuts at: 2222 2547 3005 4829 2222 Size: 325 458 1824 4093 Fragments arranged by size: 4093 1824 458 325 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4508 276 Size: 53 83 186 936 2974 2468 Fragments arranged by size: 2974 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI