Restriction map for
pFN21AA1868
(Linear) (Six-base) MAPSORT of: KIAA1868.seq Check: 3479 from: 1 to: 1995
KIAA1868.orf
With 182 enzymes: SgfI *
July 31, 2008 16:04 ..
Acc65I G'GTAC_C
Cuts at: 0 1985 1995
Size: 1985 10
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 982 1114 1285 1414 1995
Size: 982 132 171 129 581
Fragments arranged by size:
982 581 171 132 129
AflIII A'CryG_T
Cuts at: 0 1481 1995
Size: 1481 514
AhdI GACnn_n'nnGTC
Cuts at: 0 1410 1995
Size: 1410 585
AleI CACnn'nnGTG
Cuts at: 0 1237 1995
Size: 1237 758
AlwNI CAG_nnn'CTG
Cuts at: 0 285 648 843 1017 1137 1251 1995
Size: 285 363 195 174 120 114 744
Fragments arranged by size:
744 363 285 195 174 120 114
ApaI G_GGCC'C
Cuts at: 0 1314 1995
Size: 1314 681
ApaLI G'TGCA_C
Cuts at: 0 475 1995
Size: 475 1520
ApoI r'AATT_y
Cuts at: 0 67 289 1690 1710 1995
Size: 67 222 1401 20 285
Fragments arranged by size:
1401 285 222 67 20
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1543 1576 1995
Size: 1543 33 419
Fragments arranged by size:
1543 419 33
BanI G'GyrC_C
Cuts at: 0 906 1334 1985 1995
Size: 906 428 651 10
Fragments arranged by size:
906 651 428 10
BanII G_rGCy'C
Cuts at: 0 1314 1575 1946 1995
Size: 1314 261 371 49
Fragments arranged by size:
1314 371 261 49
BbeI G_GCGC'C
Cuts at: 0 1338 1995
Size: 1338 657
BbsI GAAGACnn'nnnn_
Cuts at: 0 80 1640 1903 1995
Size: 80 1560 263 92
Fragments arranged by size:
1560 263 92 80
BclI T'GATC_A
Cuts at: 0 779 1995
Size: 779 1216
BglI GCCn_nnn'nGGC
Cuts at: 0 904 1495 1995
Size: 904 591 500
Fragments arranged by size:
904 591 500
Bme1580I G_kGCm'C
Cuts at: 0 479 1314 1995
Size: 479 835 681
Fragments arranged by size:
835 681 479
BmgBI CAC'GTC
Cuts at: 0 1155 1995
Size: 1155 840
BmrI ACTGGGnnnn_n'
Cuts at: 0 1984 1995
Size: 1984 11
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 500 781 1067 1273 1447 1995
Size: 415 85 281 286 206 174 548
Fragments arranged by size:
548 415 286 281 206 174 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 435 1995
Size: 435 1560
BsaI GGTCTCn'nnnn_
Cuts at: 0 200 336 391 1054 1995
Size: 200 136 55 663 941
Fragments arranged by size:
941 663 200 136 55
BsaHI Gr'CG_yC
Cuts at: 0 1335 1995
Size: 1335 660
BsaWI w'CCGG_w
Cuts at: 0 345 1995
Size: 345 1650
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 250 370 1134 1285 1995
Size: 250 120 764 151 710
Fragments arranged by size:
764 710 250 151 120
BseYI C'CCAG_C
Cuts at: 0 1826 1995
Size: 1826 169
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1123 1945 1995
Size: 1123 822 50
Fragments arranged by size:
1123 822 50
BsiHKAI G_wGCw'C
Cuts at: 0 242 479 1511 1575 1995
Size: 242 237 1032 64 420
Fragments arranged by size:
1032 420 242 237 64
BsmI GAATG_Cn'
Cuts at: 0 106 1995
Size: 106 1889
Bsp1286I G_dGCh'C
Cuts at: 0 242 479 1314 1511 1575 1946 1995
Size: 242 237 835 197 64 371 49
Fragments arranged by size:
835 371 242 237 197 64 49
BspHI T'CATG_A
Cuts at: 0 18 1448 1880 1995
Size: 18 1430 432 115
Fragments arranged by size:
1430 432 115 18
BsrBI CCG'CTC
Cuts at: 0 1934 1995
Size: 1934 61
BsrDI GCAATG_nn'
Cuts at: 0 1092 1628 1671 1995
Size: 1092 536 43 324
Fragments arranged by size:
1092 536 324 43
BsrGI T'GTAC_A
Cuts at: 0 1577 1995
Size: 1577 418
BssSI C'ACGA_G
Cuts at: 0 864 1146 1504 1995
Size: 864 282 358 491
Fragments arranged by size:
864 491 358 282
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1017 1995
Size: 1017 978
BstXI CCAn_nnnn'nTGG
Cuts at: 0 471 1995
Size: 471 1524
BstYI r'GATC_y
Cuts at: 0 142 180 698 1524 1995
Size: 142 38 518 826 471
Fragments arranged by size:
826 518 471 142 38
BtgI C'CryG_G
Cuts at: 0 1862 1995
Size: 1862 133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1366 1995
Size: 1366 629
BtsI GCAGTG_nn'
Cuts at: 0 1933 1995
Size: 1933 62
DraI TTT'AAA
Cuts at: 0 489 1995
Size: 489 1506
EaeI y'GGCC_r
Cuts at: 0 1172 1533 1971 1995
Size: 1172 361 438 24
Fragments arranged by size:
1172 438 361 24
EarI CTCTTCn'nnn_
Cuts at: 0 502 1712 1766 1995
Size: 502 1210 54 229
Fragments arranged by size:
1210 502 229 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 155 802 1669 1920 1995
Size: 155 647 867 251 75
Fragments arranged by size:
867 647 251 155 75
EcoICRI GAG'CTC
Cuts at: 0 1573 1995
Size: 1573 422
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 500 781 982 1067 1114 1273 1285
Size: 415 85 281 201 85 47 159 12
Cuts at: 1285 1414 1447 1995
Size: 129 33 548
Fragments arranged by size:
548 415 281 201 159 129 85 85
47 33 12
EcoO109I rG'GnC_Cy
Cuts at: 0 1310 1311 1398 1995
Size: 1310 1 87 597
Fragments arranged by size:
1310 597 87 1
EcoRI G'AATT_C
Cuts at: 0 289 1690 1995
Size: 289 1401 305
Fragments arranged by size:
1401 305 289
EcoRV GAT'ATC
Cuts at: 0 1284 1995
Size: 1284 711
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 237 1995
Size: 237 1758
HaeII r_GCGC'y
Cuts at: 0 1029 1338 1995
Size: 1029 309 657
Fragments arranged by size:
1029 657 309
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 145 177 253 285 1396 1428 1995
Size: 145 32 76 32 1111 32 567
Fragments arranged by size:
1111 567 145 76 32 32 32
HindIII A'AGCT_T
Cuts at: 0 580 1852 1995
Size: 580 1272 143
Fragments arranged by size:
1272 580 143
KasI G'GCGC_C
Cuts at: 0 1334 1995
Size: 1334 661
KpnI G_GTAC'C
Cuts at: 0 1989 1995
Size: 1989 6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 1995
Size: 515 1480
MscI TGG'CCA
Cuts at: 0 1174 1535 1995
Size: 1174 361 460
Fragments arranged by size:
1174 460 361
MslI CAynn'nnrTG
Cuts at: 0 1237 1995
Size: 1237 758
MspA1I CmG'CkG
Cuts at: 0 648 770 1341 1995
Size: 648 122 571 654
Fragments arranged by size:
654 648 571 122
NarI GG'CG_CC
Cuts at: 0 1335 1995
Size: 1335 660
NspI r_CATG'y
Cuts at: 0 1485 1995
Size: 1485 510
PciI A'CATG_T
Cuts at: 0 1481 1995
Size: 1481 514
PflMI CCAn_nnn'nTGG
Cuts at: 0 285 648 1995
Size: 285 363 1347
Fragments arranged by size:
1347 363 285
PfoI T'CCnGG_A
Cuts at: 0 500 508 1044 1995
Size: 500 8 536 951
Fragments arranged by size:
951 536 500 8
PpuMI rG'GwC_Cy
Cuts at: 0 1398 1995
Size: 1398 597
PspOMI G'GGCC_C
Cuts at: 0 1310 1995
Size: 1310 685
PstI C_TGCA'G
Cuts at: 0 1247 1319 1346 1995
Size: 1247 72 27 649
Fragments arranged by size:
1247 649 72 27
PvuII CAG'CTG
Cuts at: 0 648 1995
Size: 648 1347
SacI G_AGCT'C
Cuts at: 0 1575 1995
Size: 1575 420
SapI GCTCTTCn'nnn_
Cuts at: 0 1712 1995
Size: 1712 283
ScaI AGT'ACT
Cuts at: 0 331 1432 1995
Size: 331 1101 563
Fragments arranged by size:
1101 563 331
SfcI C'TryA_G
Cuts at: 0 1243 1315 1342 1995
Size: 1243 72 27 653
Fragments arranged by size:
1243 653 72 27
SfoI GGC'GCC
Cuts at: 0 1336 1995
Size: 1336 659
SmlI C'TyrA_G
Cuts at: 0 414 1995
Size: 414 1581
SpeI A'CTAG_T
Cuts at: 0 39 1995
Size: 39 1956
StuI AGG'CCT
Cuts at: 0 870 1502 1995
Size: 870 632 493
Fragments arranged by size:
870 632 493
StyI C'CwwG_G
Cuts at: 0 902 944 1490 1995
Size: 902 42 546 505
Fragments arranged by size:
902 546 505 42
TatI w'GTAC_w
Cuts at: 0 329 1166 1430 1577 1995
Size: 329 837 264 147 418
Fragments arranged by size:
837 418 329 264 147
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1026 1058 1995
Size: 1026 32 937
Fragments arranged by size:
1026 937 32
XbaI T'CTAG_A
Cuts at: 0 753 1995
Size: 753 1242
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 721 1152 1869 1995
Size: 721 431 717 126
Fragments arranged by size:
721 717 431 126
XmnI GAAnn'nnTTC
Cuts at: 0 217 542 1000 1995
Size: 217 325 458 995
Fragments arranged by size:
995 458 325 217
Enzymes that do cut:
Acc65I AcuI AflIII AhdI AleI AlwNI ApaI ApaLI
ApoI BaeI BanI BanII BbeI BbsI BclI BglI
Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaHI BsaWI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrBI
BsrDI BsrGI BssSI BstAPI BstXI BstYI BtgI BtgZI
BtsI DraI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I HindIII KasI KpnI
MmeI MscI MslI MspA1I NarI NspI PciI PflMI
PfoI PpuMI PspOMI PstI PvuII SacI SapI ScaI
SfcI SfoI SmlI SpeI StuI StyI TatI TstI
XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AlfI
AloI AscI AseI AsiSI AvaI AvrII BamHI BbvCI
BcgI BciVI BfrBI BglII BlpI BmtI BplI Bpu10I
BsaAI BsaBI BsaXI BsiEI BsiWI BsmBI BspEI BspMI
BsrFI BssHII BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DraIII DrdI EagI EcoNI FseI FspI FspAI HincII
HpaI MfeI MluI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI
SphI SrfI SspI SwaI TaqII Tth111I XhoI XmaI
ZraI