Restriction map for
pFN21AA1866
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# NO CUT ENZYMES ( 64 enzymes) #
##################################
AccIII AclI AgeI AscI AseI
AsnI AspI AviII AvrII BclI
BlnI BseAI BsgI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
Bst1107I BstAPI BstBI BstXI BstZ17I
ClaI CpoI EcoRI EcoRV FbaI
FseI FspI HpaI MfeI MroI
MunI NdeI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SapI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AatII 4341 GACGTC GACGT^C
Acc65I 3767 GGTACC G^GTACC
AflII 2379 CTTAAG C^TTAAG
Alw44I 1714 GTGCAC G^TGCAC
ApaLI 1714 GTGCAC G^TGCAC
Asp718I 3767 GGTACC G^GTACC
BbvCI 1790 CCTCAGC CCTCAGC(-5/-2)
BciVI 2276 GTATCC GTATCC(6/5)
BfrI 2379 CTTAAG C^TTAAG
BglII 3834 AGATCT A^GATCT
BlpI 2265 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2265 GCTTAGC GC^T[ACGT]AGC
BsaBI 2458 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 341 TCCGGT [AT]^CCGG[AT]
BsrFI 1740 GCCGGC [GA]^CCGG[CT]
BssSI 4806 CACGAG CACGAG(-5/-1)
CelII 2265 GCTTAGC GC^T[ACGT]AGC
Cfr10I 1740 GCCGGC [GA]^CCGG[CT]
DrdI 637 GACATCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2649 CGGCCG C^GGCCG
EclXI 2649 CGGCCG C^GGCCG
Eco52I 2649 CGGCCG C^GGCCG
EcoT22I 3562 ATGCAT ATGCA^T
KpnI 3767 GGTACC GGTAC^C
KspI 3490 CCGCGG CCGC^GG
MamI 2458 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1740 GCCGGC GCC^GGC
NgoMIV 1740 GCCGGC G^CCGGC
NheI 507 GCTAGC G^CTAGC
NsiI 3562 ATGCAT ATGCA^T
SacII 3490 CCGCGG CCGC^GG
ScaI 2477 AGTACT AGT^ACT
SspI 1191 AATATT AAT^ATT
StuI 4304 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 28 enzymes) #
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AflIII 1523 ACGCGT A^C[GA][CT]GT
2579 ACGCGT
AocI 1350 CCTGAGG CC^T[ACGT]AGG
2467 CCTCAGG
Aor51HI 1422 AGCGCT AGC^GCT
3448 AGCGCT
Asp700I 599 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3232 GAAGACCTTC
BcgI 4025 CGACGCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2903 GCACAGAAGTCG
BfaI 508 CTAG C^TAG
3808 CTAG
BsiEI 2649 CGGCCG CG[GA][CT]^CG
3433 CGACCG
BsmI 111 GAATGC GAATGC(1/-1)
3753 GAATGC
BsrBI 2796 CCGCTC CCGCTC(-3/-3)
1994 GAGCGG
BsrDI 66 CATTGC GCAATG(2/0)
5128 CATTGC
BstEII 2732 GGTCACC G^GT[ACGT]ACC
3022 GGTGACC
BstPI 2732 GGTCACC G^GT[ACGT]ACC
3022 GGTGACC
Bsu36I 1350 CCTGAGG CC^T[ACGT]AGG
2467 CCTCAGG
DraI 3217 TTTAAA TTT^AAA
4819 TTTAAA
DraIII 857 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
1375 CACTCGGTG
Eco47III 1422 AGCGCT AGC^GCT
3448 AGCGCT
Eco81I 1350 CCTGAGG CC^T[ACGT]AGG
2467 CCTCAGG
EcoO65I 2732 GGTCACC G^GT[ACGT]ACC
3022 GGTGACC
HincII 1263 GTCGAC GT[CT]^[GA]AC
2205 GTCGAC
HindII 1263 GTCGAC GT[CT]^[GA]AC
2205 GTCGAC
HindIII 2263 AAGCTT A^AGCTT
4760 AAGCTT
MaeI 508 CTAG C^TAG
3808 CTAG
MluI 1523 ACGCGT A^CGCGT
2579 ACGCGT
PshAI 1977 GACGCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2722 GACGCGGGTC
PvuII 2963 CAGCTG CAG^CTG
5106 CAGCTG
SalI 1263 GTCGAC G^TCGAC
2205 GTCGAC
SphI 3564 GCATGC GCATG^C
4605 GCATGC
XmnI 599 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3232 GAAGACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AhdI AluI AlwI AlwNI
ApaI ApoI AspEI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvI BcnI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrI BssHII
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI Eam1105I EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI