(Circular) (Six-base) MAPSORT of: pFN21AA1866.seq Check: 4166 from: 1 to: 10024 pFN21AA1866 10024 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4984 5012 4984 Size: 28 9996 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6350 7834 278 Size: 53 83 186 936 4814 1484 2468 Fragments arranged by size: 4814 2468 1484 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2050 2580 3269 3370 4211 7367 1937 Size: 113 530 689 101 841 3156 4594 Fragments arranged by size: 4594 3156 841 689 530 113 101 Acc65I G'GTAC_C Cuts at: 5772 7345 5772 Size: 1573 8451 AclI AA'CG_TT Cuts at: 1563 8151 8524 1563 Size: 6588 373 3063 Fragments arranged by size: 6588 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3248 6276 6456 6605 8087 9471 9739 1632 Size: 1616 3028 180 149 1482 1384 268 1917 Fragments arranged by size: 3028 1917 1616 1482 1384 268 180 149 AfeI AGC'GCT Cuts at: 3429 5455 3429 Size: 2026 7998 AflII C'TTAA_G Cuts at: 820 1017 4384 820 Size: 197 3367 6460 Fragments arranged by size: 6460 3367 197 AflIII A'CryG_T Cuts at: 1203 3528 4584 8923 9900 1203 Size: 2325 1056 4339 977 1327 Fragments arranged by size: 4339 2325 1327 1056 977 AgeI A'CCGG_T Cuts at: 8855 8855 Size: 10024 AhdI GACnn_n'nnGTC Cuts at: 2365 5035 5245 8753 2365 Size: 2670 210 3508 3636 Fragments arranged by size: 3636 3508 2670 210 AleI CACnn'nnGTG Cuts at: 1166 6632 1166 Size: 5466 4558 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 10024 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4433 4465 6740 6772 4433 Size: 32 2275 32 7685 Fragments arranged by size: 7685 2275 32 32 AlwNI CAG_nnn'CTG Cuts at: 2834 2918 2936 3011 4081 7767 9339 2834 Size: 84 18 75 1070 3686 1572 3519 Fragments arranged by size: 3686 3519 1572 1070 84 75 18 ApaI G_GGCC'C Cuts at: 3418 4090 4663 4939 3418 Size: 672 573 276 8503 Fragments arranged by size: 8503 672 573 276 ApaLI G'TGCA_C Cuts at: 3719 8081 9237 3719 Size: 4362 1156 4506 Fragments arranged by size: 4506 4362 1156 ApoI r'AATT_y Cuts at: 1493 2843 6338 6443 7333 7559 7711 9820 1493 Size: 1350 3495 105 890 226 152 2109 1697 Fragments arranged by size: 3495 2109 1697 1350 890 226 152 105 AseI AT'TA_AT Cuts at: 160 1032 8578 160 Size: 872 7546 1606 Fragments arranged by size: 7546 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10024 AvaI C'yCGr_G Cuts at: 1870 1943 3303 4224 4368 4708 5103 6055 7349 Size: 73 1360 921 144 340 395 952 1294 Cuts at: 7349 1870 Size: 4545 Fragments arranged by size: 4545 1360 1294 952 921 395 340 144 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 6279 6312 7282 7315 6279 Size: 33 970 33 8988 Fragments arranged by size: 8988 970 33 33 BamHI G'GATC_C Cuts at: 2692 6237 6942 7354 7721 2692 Size: 3545 705 412 367 4995 Fragments arranged by size: 4995 3545 705 412 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2383 3214 3493 3542 3641 Size: 325 217 627 596 831 279 49 99 Cuts at: 3641 5480 5772 6416 7345 8800 618 Size: 1839 292 644 929 1455 1842 Fragments arranged by size: 1842 1839 1455 929 831 644 627 596 325 292 279 217 99 49 BanII G_rGCy'C Cuts at: 1391 3112 3418 3626 3872 4090 4231 4663 4838 Size: 1721 306 208 246 218 141 432 175 Cuts at: 4838 4939 5471 6230 7343 1391 Size: 101 532 759 1113 4072 Fragments arranged by size: 4072 1721 1113 759 532 432 306 246 218 208 175 141 101 BbeI G_GCGC'C Cuts at: 3218 3497 3645 6420 3218 Size: 279 148 2775 6822 Fragments arranged by size: 6822 2775 279 148 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2890 4050 5241 5244 6741 7134 928 Size: 971 991 1160 1191 3 1497 393 3818 Fragments arranged by size: 3818 1497 1191 1160 991 971 393 3 BbvCI CC'TCA_GC Cuts at: 3796 3796 Size: 10024 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4897 4931 6019 6053 8215 8249 1903 Size: 34 2960 34 1088 34 2162 34 3678 Fragments arranged by size: 3678 2960 2162 1088 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4292 7916 9126 4292 Size: 3624 1210 5190 Fragments arranged by size: 5190 3624 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 10024 BfrBI ATG'CAT Cuts at: 5569 5569 Size: 10024 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3035 3245 3746 Size: 107 122 71 631 605 1363 210 501 Cuts at: 3746 4502 4964 5082 5266 8635 136 Size: 756 462 118 184 3369 1525 Fragments arranged by size: 3369 1525 1363 756 631 605 501 462 210 184 122 118 107 71 BglII A'GATC_T Cuts at: 5839 10019 5839 Size: 4180 5844 BlpI GC'TnA_GC Cuts at: 4271 7441 4271 Size: 3170 6854 Bme1580I G_kGCm'C Cuts at: 1790 2322 3418 3545 3723 4090 4221 4663 4939 Size: 532 1096 127 178 367 131 442 276 Cuts at: 4939 5483 8085 9241 1790 Size: 544 2602 1156 2573 Fragments arranged by size: 2602 2573 1156 1096 544 532 442 367 276 178 131 127 BmgBI CAC'GTC Cuts at: 1318 4695 4791 5428 1318 Size: 3377 96 637 5914 Fragments arranged by size: 5914 3377 637 96 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3291 4623 5086 Size: 416 53 83 399 24 1874 1332 463 Cuts at: 5086 6682 7755 8713 442 Size: 1596 1073 958 1753 Fragments arranged by size: 1874 1753 1596 1332 1073 958 463 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2516 1056 Size: 1460 8564 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2146 3109 4865 5388 2146 Size: 963 1756 523 6782 Fragments arranged by size: 6782 1756 963 523 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2259 2418 3096 4180 4336 4822 4847 5385 Size: 1020 159 678 1084 156 486 25 538 Cuts at: 5385 8684 1239 Size: 3299 2579 Fragments arranged by size: 3299 2579 1084 1020 678 538 486 159 156 25 Bpu10I CC'TnA_GC Cuts at: 3084 3796 3866 4266 4276 4506 3084 Size: 712 70 400 10 230 8602 Fragments arranged by size: 8602 712 400 230 70 10 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3793 5697 6747 8149 9014 9312 3793 Size: 1904 1050 1402 865 298 4505 Fragments arranged by size: 4505 1904 1402 1050 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2542 3994 4155 5188 6880 7309 8687 Size: 617 1043 1452 161 1033 1692 429 1378 Cuts at: 8687 882 Size: 2219 Fragments arranged by size: 2219 1692 1452 1378 1043 1033 617 429 161 BsaAI yAC'GTr Cuts at: 493 1204 2022 4339 6484 7011 7815 493 Size: 711 818 2317 2145 527 804 2702 Fragments arranged by size: 2702 2317 2145 818 804 711 527 BsaBI GATnn'nnATC Cuts at: 4467 10018 4467 Size: 5551 4473 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3215 3494 3642 3906 Size: 53 83 186 936 1682 279 148 264 Cuts at: 3906 5301 6032 6097 6347 6417 7831 8213 8848 Size: 1395 731 65 250 70 1414 382 635 Cuts at: 8848 275 Size: 1451 Fragments arranged by size: 1682 1451 1414 1395 936 731 635 382 279 264 250 186 148 83 70 65 53 BsaWI w'CCGG_w Cuts at: 2346 8457 8855 9129 9276 9687 2346 Size: 6111 398 274 147 411 2683 Fragments arranged by size: 6111 2683 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2917 2947 5576 5606 6684 Size: 30 165 30 1514 30 2629 30 1078 Cuts at: 6684 6714 1178 Size: 30 4488 Fragments arranged by size: 4488 2629 1514 1078 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3266 3302 3305 3943 4310 4339 4477 4671 Size: 2080 36 3 638 367 29 138 194 Cuts at: 4671 4801 4804 5213 5216 5219 5832 1186 Size: 130 3 409 3 3 613 5378 Fragments arranged by size: 5378 2080 638 613 409 367 194 138 130 36 29 3 3 3 3 BseYI C'CCAG_C Cuts at: 3228 3295 3651 3674 3855 3910 4374 4698 4746 Size: 67 356 23 181 55 464 324 48 Cuts at: 4746 5040 5072 5111 5172 6606 6968 7113 9227 Size: 294 32 39 61 1434 362 145 2114 Cuts at: 9227 9723 3228 Size: 496 3529 Fragments arranged by size: 3529 2114 1434 496 464 362 356 324 294 181 145 67 61 55 48 39 32 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 9451 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4657 5441 7474 8235 9263 1540 Size: 275 186 2656 784 2033 761 1028 2301 Fragments arranged by size: 2656 2301 2033 1028 784 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3112 3723 3872 4526 4838 6230 6521 8085 8170 Size: 611 149 654 312 1392 291 1564 85 Cuts at: 8170 9241 3112 Size: 1071 3895 Fragments arranged by size: 3895 1564 1392 1071 654 611 312 291 149 85 BsmI GAATG_Cn' Cuts at: 2122 5764 7535 7628 2122 Size: 3642 1771 93 4518 Fragments arranged by size: 4518 3642 1771 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3964 4284 4719 6338 3964 Size: 320 435 1619 7650 Fragments arranged by size: 7650 1619 435 320 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2322 3112 3418 3545 3626 3723 3872 Size: 399 532 790 306 127 81 97 149 Cuts at: 3872 4090 4221 4231 4526 4663 4838 4939 5471 Size: 218 131 10 295 137 175 101 532 Cuts at: 5471 5483 6230 6521 7343 8085 8170 9241 1391 Size: 12 747 291 822 742 85 1071 2174 Fragments arranged by size: 2174 1071 822 790 747 742 532 532 399 306 295 291 218 175 149 137 131 127 101 97 85 81 12 10 BspEI T'CCGG_A Cuts at: 9687 9687 Size: 10024 BspHI T'CATG_A Cuts at: 7913 9643 7913 Size: 1730 8294 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3976 4149 4984 5012 6606 7379 844 Size: 3132 173 835 28 1594 773 3489 Fragments arranged by size: 3489 3132 1594 835 773 173 28 BsrBI CCG'CTC Cuts at: 1394 1823 4001 4803 7911 1394 Size: 429 2178 802 3108 3507 Fragments arranged by size: 3507 3108 2178 802 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2070 7132 8519 8693 65 Size: 1183 822 5062 1387 174 1396 Fragments arranged by size: 5062 1396 1387 1183 822 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3745 8668 8855 1700 Size: 110 1935 4923 187 2869 Fragments arranged by size: 4923 2869 1935 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 10024 BssHII G'CGCG_C Cuts at: 1925 3613 4952 4954 5509 1925 Size: 1688 1339 2 555 6440 Fragments arranged by size: 6440 1688 1339 555 2 BssSI C'ACGA_G Cuts at: 6811 8084 9096 6811 Size: 1273 1012 7739 Fragments arranged by size: 7739 1273 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 10024 BstBI TT'CG_AA Cuts at: 8828 8828 Size: 10024 BstEII G'GTnAC_C Cuts at: 1184 4737 5027 1184 Size: 3553 290 6181 Fragments arranged by size: 6181 3553 290 BstYI r'GATC_y Cuts at: 1972 2692 3259 5839 6237 6942 7354 7721 8106 Size: 720 567 2580 398 705 412 367 385 Cuts at: 8106 8123 9575 9661 9673 10019 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2580 1977 1452 720 705 567 412 398 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3356 4473 3356 Size: 1117 8907 BtgI C'CryG_G Cuts at: 513 1065 2004 3826 4024 4068 4285 4854 5495 Size: 552 939 1822 198 44 217 569 641 Cuts at: 5495 5856 6630 6639 6985 513 Size: 361 774 9 346 3552 Fragments arranged by size: 3552 1822 939 774 641 569 552 361 346 217 198 44 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5326 487 Size: 683 56 4100 5185 Fragments arranged by size: 5185 4100 683 56 BtsI GCAGTG_nn' Cuts at: 3414 6066 6288 6381 7540 8334 8354 3414 Size: 2652 222 93 1159 794 20 5084 Fragments arranged by size: 5084 2652 1159 794 222 93 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10024 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9989 DraI TTT'AAA Cuts at: 5224 6826 7328 5224 Size: 1602 502 7920 Fragments arranged by size: 7920 1602 502 DraIII CAC_nnn'GTG Cuts at: 2867 3385 2867 Size: 518 9506 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2648 9031 809 Size: 507 1332 6383 1802 Fragments arranged by size: 6383 1802 1332 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2452 2769 2954 Size: 54 1425 50 275 18 622 317 185 Cuts at: 2954 3376 3471 3659 4654 5492 5521 5913 6636 Size: 422 95 188 995 838 29 392 723 Cuts at: 6636 7471 8360 9989 8 Size: 835 889 1629 43 Fragments arranged by size: 1629 1425 995 889 838 835 723 622 422 392 317 275 188 185 95 54 50 43 29 18 EagI C'GGCC_G Cuts at: 1537 1812 4654 7471 1537 Size: 275 2842 2817 4090 Fragments arranged by size: 4090 2842 2817 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2610 3967 5278 5319 5805 6300 6584 7114 Size: 1257 1357 1311 41 486 495 284 530 Cuts at: 7114 7954 1353 Size: 840 3423 Fragments arranged by size: 3423 1357 1311 1257 840 530 495 486 284 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3053 4065 4076 8611 8985 9131 Size: 1064 138 1464 1012 11 4535 374 146 Cuts at: 9131 387 Size: 1280 Fragments arranged by size: 4535 1464 1280 1064 1012 374 146 138 11 EcoICRI GAG'CTC Cuts at: 3110 3870 4836 6228 3110 Size: 760 966 1392 6906 Fragments arranged by size: 6906 1392 966 760 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2259 2418 3096 3248 4180 4336 4822 Size: 393 627 159 678 152 932 156 486 Cuts at: 4822 4847 5385 6276 6456 6605 8087 8684 9471 Size: 25 538 891 180 149 1482 597 787 Cuts at: 9471 9739 1239 Size: 268 1524 Fragments arranged by size: 1524 1482 932 891 787 678 627 597 538 486 393 268 180 159 156 152 149 25 EcoNI CCTnn'n_nnAGG Cuts at: 2141 4351 5318 5358 2141 Size: 2210 967 40 6807 Fragments arranged by size: 6807 2210 967 40 EcoO109I rG'GnC_Cy Cuts at: 2026 3169 3414 3756 4178 4660 4712 4936 5037 Size: 1143 245 342 422 482 52 224 101 Cuts at: 5037 5107 5418 5850 6186 6918 7172 2026 Size: 70 311 432 336 732 254 4878 Fragments arranged by size: 4878 1143 732 482 432 422 342 336 311 254 245 224 101 70 52 EcoRI G'AATT_C Cuts at: 7333 7711 7333 Size: 378 9646 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3478 3661 5258 3478 Size: 183 1597 8244 Fragments arranged by size: 8244 1597 183 FspI TGC'GCA Cuts at: 8530 8530 Size: 10024 HaeII r_GCGC'y Cuts at: 1678 3218 3431 3497 3645 5126 5457 6420 9171 Size: 1540 213 66 148 1481 331 963 2751 Cuts at: 9171 1678 Size: 2531 Fragments arranged by size: 2751 2531 1540 1481 963 331 213 148 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2351 2383 3251 3283 3698 Size: 32 135 32 779 32 868 32 415 Cuts at: 3698 3730 5021 5053 5231 5263 6254 6286 6684 Size: 32 1291 32 178 32 991 32 398 Cuts at: 6684 6716 8665 8697 8739 8771 1373 Size: 32 1949 32 42 32 2626 Fragments arranged by size: 2626 1949 1291 991 868 779 415 398 178 135 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3270 4212 7368 7614 669 Size: 988 281 1332 942 3156 246 3079 Fragments arranged by size: 3156 3079 1332 988 942 281 246 HindIII A'AGCT_T Cuts at: 748 4268 6765 748 Size: 3520 2497 4007 Fragments arranged by size: 4007 3520 2497 HpaI GTT'AAC Cuts at: 7614 7614 Size: 10024 KasI G'GCGC_C Cuts at: 3214 3493 3641 6416 3214 Size: 279 148 2775 6822 Fragments arranged by size: 6822 2775 279 148 KpnI G_GTAC'C Cuts at: 5776 7349 5776 Size: 1573 8451 MfeI C'AATT_G Cuts at: 7623 7623 Size: 10024 MluI A'CGCG_T Cuts at: 3528 4584 9900 3528 Size: 1056 5316 3652 Fragments arranged by size: 5316 3652 1056 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5115 6125 9138 9322 1305 Size: 3810 1010 3013 184 2007 Fragments arranged by size: 3810 3013 2007 1010 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2771 2956 3473 5523 5915 Size: 54 1425 343 939 185 517 2050 392 Cuts at: 5915 10 Size: 4119 Fragments arranged by size: 4119 2050 1425 939 517 392 343 185 54 MslI CAynn'nnrTG Cuts at: 518 1166 2111 4629 5540 6008 6204 6629 6632 Size: 648 945 2518 911 468 196 425 3 Cuts at: 6632 7158 7982 8341 8500 518 Size: 526 824 359 159 2042 Fragments arranged by size: 2518 2042 945 911 824 648 526 468 425 359 196 159 3 MspA1I CmG'CkG Cuts at: 1606 4970 5497 5959 7113 7440 8117 9265 9510 Size: 3364 527 462 1154 327 677 1148 245 Cuts at: 9510 1606 Size: 2120 Fragments arranged by size: 3364 2120 1154 1148 677 527 462 327 245 NaeI GCC'GGC Cuts at: 3747 3747 Size: 10024 NarI GG'CG_CC Cuts at: 3215 3494 3642 6417 3215 Size: 279 148 2775 6822 Fragments arranged by size: 6822 2775 279 148 NcoI C'CATG_G Cuts at: 513 1065 2004 3826 4285 6985 513 Size: 552 939 1822 459 2700 3552 Fragments arranged by size: 3552 2700 1822 939 552 459 NdeI CA'TA_TG Cuts at: 387 387 Size: 10024 NgoMIV G'CCGG_C Cuts at: 3745 3745 Size: 10024 NheI G'CTAG_C Cuts at: 1052 2512 1052 Size: 1460 8564 NotI GC'GGCC_GC Cuts at: 7471 7471 Size: 10024 NsiI A_TGCA'T Cuts at: 5571 5571 Size: 10024 NspI r_CATG'y Cuts at: 1132 1229 5054 5573 6614 7382 8927 1132 Size: 97 3825 519 1041 768 1545 2229 Fragments arranged by size: 3825 2229 1545 1041 768 519 97 PasI CC'CwG_GG Cuts at: 1348 3488 3538 4236 4885 5286 5415 6182 1348 Size: 2140 50 698 649 401 129 767 5190 Fragments arranged by size: 5190 2140 767 698 649 401 129 50 PciI A'CATG_T Cuts at: 8923 8923 Size: 10024 PflMI CCAn_nnn'nTGG Cuts at: 1108 2963 3385 3827 4996 5885 7144 1108 Size: 1855 422 442 1169 889 1259 3988 Fragments arranged by size: 3988 1855 1259 1169 889 442 422 PfoI T'CCnGG_A Cuts at: 2281 3977 2281 Size: 1696 8328 PmeI GTTT'AAAC Cuts at: 7328 7328 Size: 10024 PmlI CAC'GTG Cuts at: 2022 4339 7011 2022 Size: 2317 2672 5035 Fragments arranged by size: 5035 2672 2317 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7159 7191 8093 8125 9606 9638 7159 Size: 32 902 32 1481 32 7545 Fragments arranged by size: 7545 1481 902 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2026 3169 3756 4178 4712 5037 5107 5850 6186 Size: 1143 587 422 534 325 70 743 336 Cuts at: 6186 2026 Size: 5864 Fragments arranged by size: 5864 1143 743 587 534 422 336 325 70 PshAI GACnn'nnGTC Cuts at: 1613 3986 4731 1613 Size: 2373 745 6906 Fragments arranged by size: 6906 2373 745 PsiI TTA'TAA Cuts at: 2784 5631 7594 2784 Size: 2847 1963 5214 Fragments arranged by size: 5214 2847 1963 PspOMI G'GGCC_C Cuts at: 3414 4086 4659 4935 3414 Size: 672 573 276 8503 Fragments arranged by size: 8503 672 573 276 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 7007 7039 7007 Size: 32 9992 PstI C_TGCA'G Cuts at: 830 2188 2547 3409 4191 6295 7376 830 Size: 1358 359 862 782 2104 1081 3478 Fragments arranged by size: 3478 2104 1358 1081 862 782 359 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10024 PvuII CAG'CTG Cuts at: 4970 7113 4970 Size: 2143 7881 SacI G_AGCT'C Cuts at: 3112 3872 4838 6230 3112 Size: 760 966 1392 6906 Fragments arranged by size: 6906 1392 966 760 SacII CC_GC'GG Cuts at: 5498 5498 Size: 10024 SalI G'TCGA_C Cuts at: 1936 3268 4210 7366 1936 Size: 1332 942 3156 4594 Fragments arranged by size: 4594 3156 1332 942 SanDI GG'GwC_CC Cuts at: 2026 4712 5107 5850 2026 Size: 2686 395 743 6200 Fragments arranged by size: 6200 2686 743 395 SbfI CC_TGCA'GG Cuts at: 7376 7376 Size: 10024 ScaI AGT'ACT Cuts at: 4484 8272 9984 4484 Size: 3788 1712 4524 Fragments arranged by size: 4524 3788 1712 SfcI C'TryA_G Cuts at: 826 1045 2184 2543 2581 2973 3405 4187 6291 Size: 219 1139 359 38 392 432 782 2104 Cuts at: 6291 7096 7372 8507 9188 9379 826 Size: 805 276 1135 681 191 1471 Fragments arranged by size: 2104 1471 1139 1135 805 782 681 432 392 359 276 219 191 38 SfoI GGC'GCC Cuts at: 3216 3495 3643 6418 3216 Size: 279 148 2775 6822 Fragments arranged by size: 6822 2775 279 148 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10024 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10024 SmaI CCC'GGG Cuts at: 1872 3305 4370 4710 5105 7351 1872 Size: 1433 1065 340 395 2246 4545 Fragments arranged by size: 4545 2246 1433 1065 395 340 SmlI C'TyrA_G Cuts at: 820 1017 1943 3808 4384 5712 6762 8128 9029 Size: 197 926 1865 576 1328 1050 1366 901 Cuts at: 9029 9291 820 Size: 262 1553 Fragments arranged by size: 1865 1553 1366 1328 1050 926 901 576 262 197 SnaBI TAC'GTA Cuts at: 493 7815 493 Size: 7322 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10024 SphI G_CATG'C Cuts at: 1132 5573 6614 7382 1132 Size: 4441 1041 768 3774 Fragments arranged by size: 4441 3774 1041 768 SspI AAT'ATT Cuts at: 5 52 3198 7948 5 Size: 47 3146 4750 2081 Fragments arranged by size: 4750 3146 2081 47 StuI AGG'CCT Cuts at: 1517 6311 1517 Size: 4794 5230 StyI C'CwwG_G Cuts at: 513 1065 2004 3151 3165 3636 3826 3882 4285 Size: 552 939 1147 14 471 190 56 403 Cuts at: 4285 4500 4959 5319 6189 6985 7463 513 Size: 215 459 360 870 796 478 3074 Fragments arranged by size: 3074 1147 939 870 796 552 478 471 459 403 360 215 190 56 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2047 2677 3350 3551 3869 4725 5170 Size: 1255 467 630 673 201 318 856 445 Cuts at: 5170 5498 6005 6862 8051 8068 8221 8406 8851 Size: 328 507 857 1189 17 153 185 445 Cuts at: 8851 325 Size: 1498 Fragments arranged by size: 1498 1255 1189 857 856 673 630 507 467 445 445 328 318 201 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2166 2736 4482 Size: 274 80 33 51 1443 189 570 1746 Cuts at: 4482 8270 9982 96 Size: 3788 1712 138 Fragments arranged by size: 3788 1746 1712 1443 570 274 189 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3515 3547 3883 3915 4241 4273 4328 4360 5417 Size: 32 336 32 326 32 55 32 1057 Cuts at: 5417 5449 6082 6114 9984 10016 3515 Size: 32 633 32 3870 32 3523 Fragments arranged by size: 3870 3523 1057 633 336 326 55 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 10024 XbaI T'CTAG_A Cuts at: 7360 7360 Size: 10024 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2053 5786 5839 2053 Size: 3733 53 6238 Fragments arranged by size: 6238 3733 53 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10024 XmaI C'CCGG_G Cuts at: 1870 3303 4368 4708 5103 7349 1870 Size: 1433 1065 340 395 2246 4545 Fragments arranged by size: 4545 2246 1433 1065 395 340 XmnI GAAnn'nnTTC Cuts at: 2608 5241 8153 2608 Size: 2633 2912 4479 Fragments arranged by size: 4479 2912 2633 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6348 7832 276 Size: 53 83 186 936 4814 1484 2468 Fragments arranged by size: 4814 2468 1484 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BsiWI BstXI BstZ17I EcoRV FseI FspAI NruI PacI PspXI RsrII SapI SexAI SfiI SrfI SwaI