Restriction map for
pFN21AA1866
(Linear) (Six-base) MAPSORT of: KIAA1866.seq Check: 5636 from: 1 to: 5319
KIAA1866.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2979 3007 5319
Size: 2979 28 2312
Fragments arranged by size:
2979 2312 28
AatII G_ACGT'C
Cuts at: 0 4345 5319
Size: 4345 974
AccI GT'mk_AC
Cuts at: 0 45 575 1264 1365 2206 5319
Size: 45 530 689 101 841 3113
Fragments arranged by size:
3113 841 689 530 101 45
Acc65I G'GTAC_C
Cuts at: 0 3767 5319
Size: 3767 1552
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1243 4271 4451 4600 5319
Size: 1243 3028 180 149 719
Fragments arranged by size:
3028 1243 719 180 149
AfeI AGC'GCT
Cuts at: 0 1424 3450 5319
Size: 1424 2026 1869
Fragments arranged by size:
2026 1869 1424
AflII C'TTAA_G
Cuts at: 0 2379 5319
Size: 2379 2940
AflIII A'CryG_T
Cuts at: 0 1523 2579 5319
Size: 1523 1056 2740
Fragments arranged by size:
2740 1523 1056
AhdI GACnn_n'nnGTC
Cuts at: 0 360 3030 3240 5319
Size: 360 2670 210 2079
Fragments arranged by size:
2670 2079 360 210
AleI CACnn'nnGTG
Cuts at: 0 4627 5319
Size: 4627 692
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2428 2460 4735 4767 5319
Size: 2428 32 2275 32 552
Fragments arranged by size:
2428 2275 552 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 829 913 931 1006 2076 5319
Size: 829 84 18 75 1070 3243
Fragments arranged by size:
3243 1070 829 84 75 18
ApaI G_GGCC'C
Cuts at: 0 1413 2085 2658 2934 5319
Size: 1413 672 573 276 2385
Fragments arranged by size:
2385 1413 672 573 276
ApaLI G'TGCA_C
Cuts at: 0 1714 5319
Size: 1714 3605
ApoI r'AATT_y
Cuts at: 0 838 4333 4438 5319
Size: 838 3495 105 881
Fragments arranged by size:
3495 881 838 105
AvaI C'yCGr_G
Cuts at: 0 1298 2219 2363 2703 3098 4050 5319
Size: 1298 921 144 340 395 952 1269
Fragments arranged by size:
1298 1269 952 921 395 340 144
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 4274 4307 5277 5310 5319
Size: 4274 33 970 33 9
Fragments arranged by size:
4274 970 33 33 9
BamHI G'GATC_C
Cuts at: 0 687 4232 4937 5319
Size: 687 3545 705 382
Fragments arranged by size:
3545 705 687 382
BanI G'GyrC_C
Cuts at: 0 378 1209 1488 1537 1636 3475 3767 4411
Size: 378 831 279 49 99 1839 292 644
Cuts at: 4411 5319
Size: 908
Fragments arranged by size:
1839 908 831 644 378 292 279 99
49
BanII G_rGCy'C
Cuts at: 0 1107 1413 1621 1867 2085 2226 2658 2833
Size: 1107 306 208 246 218 141 432 175
Cuts at: 2833 2934 3466 4225 5319
Size: 101 532 759 1094
Fragments arranged by size:
1107 1094 759 532 432 306 246 218
208 175 141 101
BbeI G_GCGC'C
Cuts at: 0 1213 1492 1640 4415 5319
Size: 1213 279 148 2775 904
Fragments arranged by size:
2775 1213 904 279 148
BbsI GAAGACnn'nnnn_
Cuts at: 0 885 2045 3236 3239 4736 5129 5319
Size: 885 1160 1191 3 1497 393 190
Fragments arranged by size:
1497 1191 1160 885 393 190 3
BbvCI CC'TCA_GC
Cuts at: 0 1791 5319
Size: 1791 3528
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2892 2926 4014 4048 5319
Size: 2892 34 1088 34 1271
Fragments arranged by size:
2892 1271 1088 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2287 5319
Size: 2287 3032
BfrBI ATG'CAT
Cuts at: 0 3564 5319
Size: 3564 1755
BglI GCCn_nnn'nGGC
Cuts at: 0 1030 1240 1741 2497 2959 3077 3261 5319
Size: 1030 210 501 756 462 118 184 2058
Fragments arranged by size:
2058 1030 756 501 462 210 184 118
BglII A'GATC_T
Cuts at: 0 3834 5319
Size: 3834 1485
BlpI GC'TnA_GC
Cuts at: 0 2266 5319
Size: 2266 3053
Bme1580I G_kGCm'C
Cuts at: 0 317 1413 1540 1718 2085 2216 2658 2934
Size: 317 1096 127 178 367 131 442 276
Cuts at: 2934 3478 5319
Size: 544 1841
Fragments arranged by size:
1841 1096 544 442 367 317 276 178
131 127
BmgBI CAC'GTC
Cuts at: 0 2690 2786 3423 5319
Size: 2690 96 637 1896
Fragments arranged by size:
2690 1896 637 96
BmrI ACTGGGnnnn_n'
Cuts at: 0 1286 2618 3081 4677 5319
Size: 1286 1332 463 1596 642
Fragments arranged by size:
1596 1332 1286 642 463
BmtI G_CTAG'C
Cuts at: 0 511 5319
Size: 511 4808
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 141 1104 2860 3383 5319
Size: 141 963 1756 523 1936
Fragments arranged by size:
1936 1756 963 523 141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 413 1091 2175 2331 2817 2842 3380
Size: 254 159 678 1084 156 486 25 538
Cuts at: 3380 5319
Size: 1939
Fragments arranged by size:
1939 1084 678 538 486 254 159 156
25
Bpu10I CC'TnA_GC
Cuts at: 0 1079 1791 1861 2261 2271 2501 5319
Size: 1079 712 70 400 10 230 2818
Fragments arranged by size:
2818 1079 712 400 230 70 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1788 3692 4742 5319
Size: 1788 1904 1050 577
Fragments arranged by size:
1904 1788 1050 577
BsaI GGTCTCn'nnnn_
Cuts at: 0 537 1989 2150 3183 4875 5304 5319
Size: 537 1452 161 1033 1692 429 15
Fragments arranged by size:
1692 1452 1033 537 429 161 15
BsaAI yAC'GTr
Cuts at: 0 17 2334 4479 5006 5319
Size: 17 2317 2145 527 313
Fragments arranged by size:
2317 2145 527 313 17
BsaBI GATnn'nnATC
Cuts at: 0 2462 5319
Size: 2462 2857
BsaHI Gr'CG_yC
Cuts at: 0 1210 1489 1637 1901 3296 4027 4092 4342
Size: 1210 279 148 264 1395 731 65 250
Cuts at: 4342 4412 5319
Size: 70 907
Fragments arranged by size:
1395 1210 907 731 279 264 250 148
70 65
BsaWI w'CCGG_w
Cuts at: 0 341 5319
Size: 341 4978
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 912 942 3571 3601 4679 4709 5319
Size: 912 30 2629 30 1078 30 610
Fragments arranged by size:
2629 1078 912 610 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1261 1297 1300 1938 2305 2334 2472 2666
Size: 1261 36 3 638 367 29 138 194
Cuts at: 2666 2796 2799 3208 3211 3214 3827 5319
Size: 130 3 409 3 3 613 1492
Fragments arranged by size:
1492 1261 638 613 409 367 194 138
130 36 29 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1223 1290 1646 1669 1850 1905 2369 2693
Size: 1223 67 356 23 181 55 464 324
Cuts at: 2693 2741 3035 3067 3106 3167 4601 4963 5108
Size: 48 294 32 39 61 1434 362 145
Cuts at: 5108 5319
Size: 211
Fragments arranged by size:
1434 1223 464 362 356 324 294 211
181 145 67 61 55 48 39 32
23
BsiEI CG_ry'CG
Cuts at: 0 2652 3436 5319
Size: 2652 784 1883
Fragments arranged by size:
2652 1883 784
BsiHKAI G_wGCw'C
Cuts at: 0 1107 1718 1867 2521 2833 4225 4516 5319
Size: 1107 611 149 654 312 1392 291 803
Fragments arranged by size:
1392 1107 803 654 611 312 291 149
BsmI GAATG_Cn'
Cuts at: 0 117 3759 5319
Size: 117 3642 1560
Fragments arranged by size:
3642 1560 117
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1959 2279 2714 4333 5319
Size: 1959 320 435 1619 986
Fragments arranged by size:
1959 1619 986 435 320
Bsp1286I G_dGCh'C
Cuts at: 0 317 1107 1413 1540 1621 1718 1867 2085
Size: 317 790 306 127 81 97 149 218
Cuts at: 2085 2216 2226 2521 2658 2833 2934 3466 3478
Size: 131 10 295 137 175 101 532 12
Cuts at: 3478 4225 4516 5319
Size: 747 291 803
Fragments arranged by size:
803 790 747 532 317 306 295 291
218 175 149 137 131 127 101 97
81 12 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1971 2144 2979 3007 4601 5319
Size: 1971 173 835 28 1594 718
Fragments arranged by size:
1971 1594 835 718 173 28
BsrBI CCG'CTC
Cuts at: 0 1996 2798 5319
Size: 1996 802 2521
Fragments arranged by size:
2521 1996 802
BsrDI GCAATG_nn'
Cuts at: 0 65 5127 5319
Size: 65 5062 192
Fragments arranged by size:
5062 192 65
BsrFI r'CCGG_y
Cuts at: 0 1740 5319
Size: 1740 3579
BssHII G'CGCG_C
Cuts at: 0 1608 2947 2949 3504 5319
Size: 1608 1339 2 555 1815
Fragments arranged by size:
1815 1608 1339 555 2
BssSI C'ACGA_G
Cuts at: 0 4806 5319
Size: 4806 513
BstEII G'GTnAC_C
Cuts at: 0 2732 3022 5319
Size: 2732 290 2297
Fragments arranged by size:
2732 2297 290
BstYI r'GATC_y
Cuts at: 0 687 1254 3834 4232 4937 5319
Size: 687 567 2580 398 705 382
Fragments arranged by size:
2580 705 687 567 398 382
Bsu36I CC'TnA_GG
Cuts at: 0 1351 2468 5319
Size: 1351 1117 2851
Fragments arranged by size:
2851 1351 1117
BtgI C'CryG_G
Cuts at: 0 1821 2019 2063 2280 2849 3490 3851 4625
Size: 1821 198 44 217 569 641 361 774
Cuts at: 4625 4634 4980 5319
Size: 9 346 339
Fragments arranged by size:
1821 774 641 569 361 346 339 217
198 44 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3321 5319
Size: 3321 1998
BtsI GCAGTG_nn'
Cuts at: 0 1409 4061 4283 4376 5319
Size: 1409 2652 222 93 943
Fragments arranged by size:
2652 1409 943 222 93
DraI TTT'AAA
Cuts at: 0 3219 4821 5319
Size: 3219 1602 498
Fragments arranged by size:
3219 1602 498
DraIII CAC_nnn'GTG
Cuts at: 0 862 1380 5319
Size: 862 518 3939
Fragments arranged by size:
3939 862 518
DrdI GACnn_nn'nnGTC
Cuts at: 0 643 5319
Size: 643 4676
EaeI y'GGCC_r
Cuts at: 0 447 764 949 1371 1466 1654 2649 3487
Size: 447 317 185 422 95 188 995 838
Cuts at: 3487 3516 3908 4631 5319
Size: 29 392 723 688
Fragments arranged by size:
995 838 723 688 447 422 392 317
188 185 95 29
EagI C'GGCC_G
Cuts at: 0 2649 5319
Size: 2649 2670
EarI CTCTTCn'nnn_
Cuts at: 0 605 1962 3273 3314 3800 4295 4579 5109
Size: 605 1357 1311 41 486 495 284 530
Cuts at: 5109 5319
Size: 210
Fragments arranged by size:
1357 1311 605 530 495 486 284 210
41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1048 2060 2071 5319
Size: 1048 1012 11 3248
Fragments arranged by size:
3248 1048 1012 11
EcoICRI GAG'CTC
Cuts at: 0 1105 1865 2831 4223 5319
Size: 1105 760 966 1392 1096
Fragments arranged by size:
1392 1105 1096 966 760
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 413 1091 1243 2175 2331 2817 2842
Size: 254 159 678 152 932 156 486 25
Cuts at: 2842 3380 4271 4451 4600 5319
Size: 538 891 180 149 719
Fragments arranged by size:
932 891 719 678 538 486 254 180
159 156 152 149 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 136 2346 3313 3353 5319
Size: 136 2210 967 40 1966
Fragments arranged by size:
2210 1966 967 136 40
EcoO109I rG'GnC_Cy
Cuts at: 0 21 1164 1409 1751 2173 2655 2707 2931
Size: 21 1143 245 342 422 482 52 224
Cuts at: 2931 3032 3102 3413 3845 4181 4913 5167 5319
Size: 101 70 311 432 336 732 254 152
Fragments arranged by size:
1143 732 482 432 422 342 336 311
254 245 224 152 101 70 52 21
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1473 1656 3253 5319
Size: 1473 183 1597 2066
Fragments arranged by size:
2066 1597 1473 183
HaeII r_GCGC'y
Cuts at: 0 1213 1426 1492 1640 3121 3452 4415 5319
Size: 1213 213 66 148 1481 331 963 904
Fragments arranged by size:
1481 1213 963 904 331 213 148 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 346 378 1246 1278 1693 1725 3016 3048
Size: 346 32 868 32 415 32 1291 32
Cuts at: 3048 3226 3258 4249 4281 4679 4711 5319
Size: 178 32 991 32 398 32 608
Fragments arranged by size:
1291 991 868 608 415 398 346 178
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1265 2207 5319
Size: 1265 942 3112
Fragments arranged by size:
3112 1265 942
HindIII A'AGCT_T
Cuts at: 0 2263 4760 5319
Size: 2263 2497 559
Fragments arranged by size:
2497 2263 559
KasI G'GCGC_C
Cuts at: 0 1209 1488 1636 4411 5319
Size: 1209 279 148 2775 908
Fragments arranged by size:
2775 1209 908 279 148
KpnI G_GTAC'C
Cuts at: 0 3771 5319
Size: 3771 1548
MluI A'CGCG_T
Cuts at: 0 1523 2579 5319
Size: 1523 1056 2740
Fragments arranged by size:
2740 1523 1056
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3110 4120 5319
Size: 3110 1010 1199
Fragments arranged by size:
3110 1199 1010
MscI TGG'CCA
Cuts at: 0 766 951 1468 3518 3910 5319
Size: 766 185 517 2050 392 1409
Fragments arranged by size:
2050 1409 766 517 392 185
MslI CAynn'nnrTG
Cuts at: 0 106 2624 3535 4003 4199 4624 4627 5153
Size: 106 2518 911 468 196 425 3 526
Cuts at: 5153 5319
Size: 166
Fragments arranged by size:
2518 911 526 468 425 196 166 106
3
MspA1I CmG'CkG
Cuts at: 0 2965 3492 3954 5108 5319
Size: 2965 527 462 1154 211
Fragments arranged by size:
2965 1154 527 462 211
NaeI GCC'GGC
Cuts at: 0 1742 5319
Size: 1742 3577
NarI GG'CG_CC
Cuts at: 0 1210 1489 1637 4412 5319
Size: 1210 279 148 2775 907
Fragments arranged by size:
2775 1210 907 279 148
NcoI C'CATG_G
Cuts at: 0 1821 2280 4980 5319
Size: 1821 459 2700 339
Fragments arranged by size:
2700 1821 459 339
NgoMIV G'CCGG_C
Cuts at: 0 1740 5319
Size: 1740 3579
NheI G'CTAG_C
Cuts at: 0 507 5319
Size: 507 4812
NsiI A_TGCA'T
Cuts at: 0 3566 5319
Size: 3566 1753
NspI r_CATG'y
Cuts at: 0 3049 3568 4609 5319
Size: 3049 519 1041 710
Fragments arranged by size:
3049 1041 710 519
PasI CC'CwG_GG
Cuts at: 0 1483 1533 2231 2880 3281 3410 4177 5319
Size: 1483 50 698 649 401 129 767 1142
Fragments arranged by size:
1483 1142 767 698 649 401 129 50
PflMI CCAn_nnn'nTGG
Cuts at: 0 958 1380 1822 2991 3880 5139 5319
Size: 958 422 442 1169 889 1259 180
Fragments arranged by size:
1259 1169 958 889 442 422 180
PfoI T'CCnGG_A
Cuts at: 0 276 1972 5319
Size: 276 1696 3347
Fragments arranged by size:
3347 1696 276
PmlI CAC'GTG
Cuts at: 0 17 2334 5006 5319
Size: 17 2317 2672 313
Fragments arranged by size:
2672 2317 313 17
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5154 5186 5319
Size: 5154 32 133
Fragments arranged by size:
5154 133 32
PpuMI rG'GwC_Cy
Cuts at: 0 21 1164 1751 2173 2707 3032 3102 3845
Size: 21 1143 587 422 534 325 70 743
Cuts at: 3845 4181 5319
Size: 336 1138
Fragments arranged by size:
1143 1138 743 587 534 422 336 325
70 21
PshAI GACnn'nnGTC
Cuts at: 0 1981 2726 5319
Size: 1981 745 2593
Fragments arranged by size:
2593 1981 745
PsiI TTA'TAA
Cuts at: 0 779 3626 5319
Size: 779 2847 1693
Fragments arranged by size:
2847 1693 779
PspOMI G'GGCC_C
Cuts at: 0 1409 2081 2654 2930 5319
Size: 1409 672 573 276 2389
Fragments arranged by size:
2389 1409 672 573 276
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 5002 5034 5319
Size: 5002 32 285
Fragments arranged by size:
5002 285 32
PstI C_TGCA'G
Cuts at: 0 183 542 1404 2186 4290 5319
Size: 183 359 862 782 2104 1029
Fragments arranged by size:
2104 1029 862 782 359 183
PvuII CAG'CTG
Cuts at: 0 2965 5108 5319
Size: 2965 2143 211
Fragments arranged by size:
2965 2143 211
SacI G_AGCT'C
Cuts at: 0 1107 1867 2833 4225 5319
Size: 1107 760 966 1392 1094
Fragments arranged by size:
1392 1107 1094 966 760
SacII CC_GC'GG
Cuts at: 0 3493 5319
Size: 3493 1826
SalI G'TCGA_C
Cuts at: 0 1263 2205 5319
Size: 1263 942 3114
Fragments arranged by size:
3114 1263 942
SanDI GG'GwC_CC
Cuts at: 0 21 2707 3102 3845 5319
Size: 21 2686 395 743 1474
Fragments arranged by size:
2686 1474 743 395 21
ScaI AGT'ACT
Cuts at: 0 2479 5319
Size: 2479 2840
SfcI C'TryA_G
Cuts at: 0 179 538 576 968 1400 2182 4286 5091
Size: 179 359 38 392 432 782 2104 805
Cuts at: 5091 5319
Size: 228
Fragments arranged by size:
2104 805 782 432 392 359 228 179
38
SfoI GGC'GCC
Cuts at: 0 1211 1490 1638 4413 5319
Size: 1211 279 148 2775 906
Fragments arranged by size:
2775 1211 906 279 148
SmaI CCC'GGG
Cuts at: 0 1300 2365 2705 3100 5319
Size: 1300 1065 340 395 2219
Fragments arranged by size:
2219 1300 1065 395 340
SmlI C'TyrA_G
Cuts at: 0 1803 2379 3707 4757 5319
Size: 1803 576 1328 1050 562
Fragments arranged by size:
1803 1328 1050 576 562
SphI G_CATG'C
Cuts at: 0 3568 4609 5319
Size: 3568 1041 710
Fragments arranged by size:
3568 1041 710
SspI AAT'ATT
Cuts at: 0 1193 5319
Size: 1193 4126
StuI AGG'CCT
Cuts at: 0 4306 5319
Size: 4306 1013
StyI C'CwwG_G
Cuts at: 0 1146 1160 1631 1821 1877 2280 2495 2954
Size: 1146 14 471 190 56 403 215 459
Cuts at: 2954 3314 4184 4980 5319
Size: 360 870 796 339
Fragments arranged by size:
1146 870 796 471 459 403 360 339
215 190 56 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 42 672 1345 1546 1864 2720 3165 3493
Size: 42 630 673 201 318 856 445 328
Cuts at: 3493 4000 4857 5319
Size: 507 857 462
Fragments arranged by size:
857 856 673 630 507 462 445 328
318 201 42
TatI w'GTAC_w
Cuts at: 0 161 731 2477 5319
Size: 161 570 1746 2842
Fragments arranged by size:
2842 1746 570 161
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1510 1542 1878 1910 2236 2268 2323 2355
Size: 1510 32 336 32 326 32 55 32
Cuts at: 2355 3412 3444 4077 4109 5319
Size: 1057 32 633 32 1210
Fragments arranged by size:
1510 1210 1057 633 336 326 55 32
32 32 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 48 3781 3834 5319
Size: 48 3733 53 1485
Fragments arranged by size:
3733 1485 53 48
XmaI C'CCGG_G
Cuts at: 0 1298 2363 2703 3098 5319
Size: 1298 1065 340 395 2221
Fragments arranged by size:
2221 1298 1065 395 340
XmnI GAAnn'nnTTC
Cuts at: 0 603 3236 5319
Size: 603 2633 2083
Fragments arranged by size:
2633 2083 603
ZraI GAC'GTC
Cuts at: 0 4343 5319
Size: 4343 976
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NsiI NspI PasI PflMI PfoI PmlI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuII
SacI SacII SalI SanDI ScaI SfcI SfoI SmaI
SmlI SphI SspI StuI StyI TaqII TatI TstI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AgeI AlfI AscI AseI AsiSI AvrII BclI
BsgI BsiWI BspEI BspHI BsrGI BstAPI BstBI BstXI
BstZ17I ClaI CspCI EcoRI EcoRV FseI FspI FspAI
HpaI MfeI NdeI NotI NruI PacI PciI PmeI
PspXI PvuI RsrII SapI SbfI SexAI SfiI SgfI
SgrAI SnaBI SpeI SrfI SwaI Tth111I XbaI XhoI