(Circular) (Six-base) MAPSORT of: pFN21AA1861.seq Check: 5648 from: 1 to: 7576 pFN21AA1861 7576 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5386 278 Size: 53 83 186 936 3850 2468 Fragments arranged by size: 3850 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3994 4537 4919 1937 Size: 2057 543 382 4594 Fragments arranged by size: 4594 2057 543 382 Acc65I G'GTAC_C Cuts at: 4897 4897 Size: 7576 AclI AA'CG_TT Cuts at: 1563 5703 6076 1563 Size: 4140 373 3063 Fragments arranged by size: 4140 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3033 3078 3102 3306 4205 4253 5639 7023 Size: 1401 45 24 204 899 48 1386 1384 Cuts at: 7023 7291 1632 Size: 268 1917 Fragments arranged by size: 1917 1401 1386 1384 899 268 204 48 45 24 AflII C'TTAA_G Cuts at: 820 1017 3866 820 Size: 197 2849 4530 Fragments arranged by size: 4530 2849 197 AflIII A'CryG_T Cuts at: 1203 2926 6475 7452 1203 Size: 1723 3549 977 1327 Fragments arranged by size: 3549 1723 1327 977 AgeI A'CCGG_T Cuts at: 6407 6407 Size: 7576 AhdI GACnn_n'nnGTC Cuts at: 6305 6305 Size: 7576 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7576 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2983 3266 4520 1184 Size: 1799 283 1254 4240 Fragments arranged by size: 4240 1799 1254 283 AlwNI CAG_nnn'CTG Cuts at: 2020 2482 3056 4366 5319 6891 2020 Size: 462 574 1310 953 1572 2705 Fragments arranged by size: 2705 1572 1310 953 574 462 ApaLI G'TGCA_C Cuts at: 5633 6789 5633 Size: 1156 6420 ApoI r'AATT_y Cuts at: 1493 2090 2650 2722 2891 3266 3412 3437 3443 Size: 597 560 72 169 375 146 25 6 Cuts at: 3443 3558 4448 4685 4885 5111 5263 7372 1493 Size: 115 890 237 200 226 152 2109 1697 Fragments arranged by size: 2109 1697 890 597 560 375 237 226 200 169 152 146 115 72 25 6 AseI AT'TA_AT Cuts at: 160 1032 2145 6130 160 Size: 872 1113 3985 1606 Fragments arranged by size: 3985 1606 1113 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7576 AvaI C'yCGr_G Cuts at: 1870 1943 3738 4901 1870 Size: 73 1795 1163 4545 Fragments arranged by size: 4545 1795 1163 73 BamHI G'GATC_C Cuts at: 4805 4906 5273 4805 Size: 101 367 7108 Fragments arranged by size: 7108 367 101 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2054 4099 4897 6352 618 Size: 325 217 627 267 2045 798 1455 1842 Fragments arranged by size: 2045 1842 1455 798 627 325 267 217 BanII G_rGCy'C Cuts at: 1391 4895 1391 Size: 3504 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2360 2410 928 Size: 971 461 50 6094 Fragments arranged by size: 6094 971 461 50 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5767 5801 1903 Size: 34 3830 34 3678 Fragments arranged by size: 3830 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3034 4138 5468 6678 3034 Size: 1104 1330 1210 3932 Fragments arranged by size: 3932 1330 1210 1104 BclI T'GATC_A Cuts at: 1548 1548 Size: 7576 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6187 136 Size: 107 122 71 631 605 4515 1525 Fragments arranged by size: 4515 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7571 7571 Size: 7576 BlpI GC'TnA_GC Cuts at: 2993 4993 2993 Size: 2000 5576 Bme1580I G_kGCm'C Cuts at: 1790 4609 5637 6793 1790 Size: 2819 1028 1156 2573 Fragments arranged by size: 2819 2573 1156 1028 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7576 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5307 6265 442 Size: 416 53 83 399 24 3890 958 1753 Fragments arranged by size: 3890 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7576 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2225 2576 6236 1239 Size: 986 351 3660 2579 Fragments arranged by size: 3660 2579 986 351 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2016 2109 2421 5701 6566 6864 2016 Size: 93 312 3280 865 298 2728 Fragments arranged by size: 3280 2728 865 312 298 93 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6239 882 Size: 617 4740 2219 Fragments arranged by size: 4740 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5367 493 Size: 711 4163 2702 Fragments arranged by size: 4163 2702 711 BsaBI GATnn'nnATC Cuts at: 7570 7570 Size: 7576 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5383 5765 6400 275 Size: 53 83 186 936 3850 382 635 1451 Fragments arranged by size: 3850 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6009 6407 6681 6828 7239 6009 Size: 398 274 147 411 6346 Fragments arranged by size: 6346 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7351 Fragments arranged by size: 7351 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2573 1186 Size: 1387 6189 BseYI C'CCAG_C Cuts at: 6779 7275 6779 Size: 496 7080 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2892 1163 Size: 573 1156 5847 Fragments arranged by size: 5847 1156 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5026 5787 6815 1540 Size: 275 186 3025 761 1028 2301 Fragments arranged by size: 3025 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4755 5637 5722 6793 4755 Size: 882 85 1071 5538 Fragments arranged by size: 5538 1071 882 85 BsmI GAATG_Cn' Cuts at: 3796 5087 5180 3796 Size: 1291 93 6192 Fragments arranged by size: 6192 1291 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4609 4755 4895 5637 5722 6793 1391 Size: 399 2819 146 140 742 85 1071 2174 Fragments arranged by size: 2819 2174 1071 742 399 146 140 85 BspEI T'CCGG_A Cuts at: 7239 7239 Size: 7576 BspHI T'CATG_A Cuts at: 3433 5465 7195 3433 Size: 2032 1730 3814 Fragments arranged by size: 3814 2032 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3234 4931 844 Size: 2390 1697 3489 Fragments arranged by size: 3489 2390 1697 BsrBI CCG'CTC Cuts at: 1394 1823 5463 1394 Size: 429 3640 3507 Fragments arranged by size: 3640 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2354 2519 2796 6071 6245 65 Size: 1183 1106 165 277 3275 174 1396 Fragments arranged by size: 3275 1396 1183 1106 277 174 165 BsrFI r'CCGG_y Cuts at: 1700 1810 6220 6407 1700 Size: 110 4410 187 2869 Fragments arranged by size: 4410 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2390 2921 96 Size: 2294 531 4751 Fragments arranged by size: 4751 2294 531 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7576 BssSI C'ACGA_G Cuts at: 5636 6648 5636 Size: 1012 6564 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7576 BstBI TT'CG_AA Cuts at: 6380 6380 Size: 7576 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7576 BstXI CCAn_nnnn'nTGG Cuts at: 4326 4326 Size: 7576 BstYI r'GATC_y Cuts at: 1972 2914 4805 4906 5273 5658 5675 7127 7213 Size: 942 1891 101 367 385 17 1452 86 Cuts at: 7213 7225 7571 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1891 1452 942 385 367 346 101 86 17 12 BstZ17I GTA'TAC Cuts at: 4538 4538 Size: 7576 Bsu36I CC'TnA_GG Cuts at: 3675 3675 Size: 7576 BtgI C'CryG_G Cuts at: 513 1065 4179 513 Size: 552 3114 3910 Fragments arranged by size: 3910 3114 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6837 Fragments arranged by size: 6837 683 56 BtsI GCAGTG_nn' Cuts at: 5092 5886 5906 5092 Size: 794 20 6762 Fragments arranged by size: 6762 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7576 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7541 DraI TTT'AAA Cuts at: 2623 3442 4880 2623 Size: 819 1438 5319 Fragments arranged by size: 5319 1438 819 DraIII CAC_nnn'GTG Cuts at: 4126 4126 Size: 7576 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6583 809 Size: 507 5267 1802 Fragments arranged by size: 5267 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5023 5912 7541 Size: 54 1425 50 275 18 3193 889 1629 Cuts at: 7541 8 Size: 43 Fragments arranged by size: 3193 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5023 1537 Size: 275 3211 4090 Fragments arranged by size: 4090 3211 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5506 1353 Size: 4153 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6163 6537 6683 387 Size: 1064 138 4574 374 146 1280 Fragments arranged by size: 4574 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2225 2576 3033 3078 3102 3306 4205 Size: 393 593 351 457 45 24 204 899 Cuts at: 4205 4253 5639 6236 7023 7291 1239 Size: 48 1386 597 787 268 1524 Fragments arranged by size: 1524 1386 899 787 597 593 457 393 351 268 204 48 45 24 EcoO109I rG'GnC_Cy Cuts at: 2074 2074 Size: 7576 EcoRI G'AATT_C Cuts at: 2090 2650 4885 5263 2090 Size: 560 2235 378 4403 Fragments arranged by size: 4403 2235 560 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2867 4046 2867 Size: 1179 6397 FspI TGC'GCA Cuts at: 6082 6082 Size: 7576 HaeII r_GCGC'y Cuts at: 1678 6723 1678 Size: 5045 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3628 3660 4797 4829 6217 Size: 32 135 32 2056 32 1137 32 1388 Cuts at: 6217 6249 6291 6323 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2056 1388 1137 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4920 5166 669 Size: 988 281 2982 246 3079 Fragments arranged by size: 3079 2982 988 281 246 HindIII A'AGCT_T Cuts at: 748 2210 2757 4707 748 Size: 1462 547 1950 3617 Fragments arranged by size: 3617 1950 1462 547 HpaI GTT'AAC Cuts at: 5166 5166 Size: 7576 KpnI G_GTAC'C Cuts at: 4901 4901 Size: 7576 MfeI C'AATT_G Cuts at: 3873 4616 5175 3873 Size: 743 559 6274 Fragments arranged by size: 6274 743 559 MluI A'CGCG_T Cuts at: 7452 7452 Size: 7576 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3238 3817 3929 6690 6874 1305 Size: 1933 579 112 2761 184 2007 Fragments arranged by size: 2761 2007 1933 579 184 112 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5754 Fragments arranged by size: 5754 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3033 3142 3576 4233 5534 5893 6052 Size: 648 1867 109 434 657 1301 359 159 Cuts at: 6052 518 Size: 2042 Fragments arranged by size: 2042 1867 1301 657 648 434 359 159 109 MspA1I CmG'CkG Cuts at: 1606 2692 4052 4777 4992 5669 6817 7062 1606 Size: 1086 1360 725 215 677 1148 245 2120 Fragments arranged by size: 2120 1360 1148 1086 725 677 245 215 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7024 NdeI CA'TA_TG Cuts at: 387 2805 387 Size: 2418 5158 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7576 NotI GC'GGCC_GC Cuts at: 5023 5023 Size: 7576 NspI r_CATG'y Cuts at: 1132 1229 2930 4934 6479 1132 Size: 97 1701 2004 1545 2229 Fragments arranged by size: 2229 2004 1701 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7576 PciI A'CATG_T Cuts at: 2926 6475 2926 Size: 3549 4027 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7576 PfoI T'CCnGG_A Cuts at: 2572 2572 Size: 7576 PmeI GTTT'AAAC Cuts at: 4880 4880 Size: 7576 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5645 5677 7158 7190 5645 Size: 32 1481 32 6031 Fragments arranged by size: 6031 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2074 2074 Size: 7576 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7576 PsiI TTA'TAA Cuts at: 5146 5146 Size: 7576 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4521 4553 4521 Size: 32 7544 PstI C_TGCA'G Cuts at: 830 2281 4252 4928 830 Size: 1451 1971 676 3478 Fragments arranged by size: 3478 1971 1451 676 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7576 PvuII CAG'CTG Cuts at: 2692 4052 4777 2692 Size: 1360 725 5491 Fragments arranged by size: 5491 1360 725 SalI G'TCGA_C Cuts at: 1936 4918 1936 Size: 2982 4594 SanDI GG'GwC_CC Cuts at: 2074 2074 Size: 7576 SbfI CC_TGCA'GG Cuts at: 4928 4928 Size: 7576 ScaI AGT'ACT Cuts at: 3533 5824 7536 3533 Size: 2291 1712 3573 Fragments arranged by size: 3573 2291 1712 SfcI C'TryA_G Cuts at: 826 1045 2277 3535 4248 4848 4924 6059 6740 Size: 219 1232 1258 713 600 76 1135 681 Cuts at: 6740 6931 826 Size: 191 1471 Fragments arranged by size: 1471 1258 1232 1135 713 681 600 219 191 76 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7576 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7576 SmaI CCC'GGG Cuts at: 1872 4903 1872 Size: 3031 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2031 2124 2436 3866 5680 6581 Size: 197 926 88 93 312 1430 1814 901 Cuts at: 6581 6843 820 Size: 262 1553 Fragments arranged by size: 1814 1553 1430 926 901 312 262 197 93 88 SnaBI TAC'GTA Cuts at: 493 5367 493 Size: 4874 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7576 SphI G_CATG'C Cuts at: 1132 4934 1132 Size: 3802 3774 SspI AAT'ATT Cuts at: 5 52 2416 4763 5500 5 Size: 47 2364 2347 737 2081 Fragments arranged by size: 2364 2347 2081 737 47 StuI AGG'CCT Cuts at: 1517 2451 1517 Size: 934 6642 StyI C'CwwG_G Cuts at: 513 1065 2745 4188 5015 513 Size: 552 1680 1443 827 3074 Fragments arranged by size: 3074 1680 1443 827 552 SwaI ATTT'AAAT Cuts at: 3442 3442 Size: 7576 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2831 5603 5620 5773 5958 6403 325 Size: 1255 1251 2772 17 153 185 445 1498 Fragments arranged by size: 2772 1498 1255 1251 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2390 2921 3531 Size: 274 80 33 51 1443 413 531 610 Cuts at: 3531 5822 7534 96 Size: 2291 1712 138 Fragments arranged by size: 2291 1712 1443 610 531 413 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7536 7568 7536 Size: 32 7544 Tth111I GACn'n_nGTC Cuts at: 1316 2016 1316 Size: 700 6876 XbaI T'CTAG_A Cuts at: 4039 4912 4039 Size: 873 6703 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4186 4186 Size: 7576 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7576 XmaI C'CCGG_G Cuts at: 1870 4901 1870 Size: 3031 4545 XmnI GAAnn'nnTTC Cuts at: 5705 5705 Size: 7576 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5384 276 Size: 53 83 186 936 3850 2468 Fragments arranged by size: 3850 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI