Restriction map for
pFN21AA1853
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbeI BbrPI BbsI BciVI
BclI BfrI BglI BglII BlnI
BpuAI BsaAI BsaBI BsaWI BseAI
BsgI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KasI KpnI MamI
MfeI MluI MroI MseI MslI
MunI NarI NcoI NdeI NheI
NotI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
PvuII RsrII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp509I Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 1457 GACGTC GACGT^C
AcyI 1457 GACGTC G[GA]^CG[CT]C
AflIII 869 ACACGT A^C[GA][CT]GT
AlwNI 193 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 993 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1398 GGATCC G^GATCC
BbvCI 1587 CCTCAGC CCTCAGC(-5/-2)
BcgI 89 GCATCATTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 1457 GACGTC G[GA]^CG[CT]C
BsaI 908 GGTCTC GGTCTC(1/5)
BsiEI 621 CGGCCG CG[GA][CT]^CG
BspHI 285 TCATGA T^CATGA
BspMI 434 GCAGGT ACCTGC(4/8)
BstXI 738 CCAGATGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1398 GGATCC [GA]^GATC[CT]
DsaI 618 CCGCGG C^C[GA][CT]GG
EagI 621 CGGCCG C^GGCCG
EclXI 621 CGGCCG C^GGCCG
Eco52I 621 CGGCCG C^GGCCG
HaeII 1484 AGCGCC [GA]GCGC^[CT]
Hin1I 1457 GACGTC G[GA]^CG[CT]C
HincII 728 GTCAAC GT[CT]^[GA]AC
HindII 728 GTCAAC GT[CT]^[GA]AC
KspI 618 CCGCGG CCGC^GG
MflI 1398 GGATCC [GA]^GATC[CT]
NaeI 524 GCCGGC GCC^GGC
NgoMIV 524 GCCGGC G^CCGGC
NruI 832 TCGCGA TCG^CGA
PflMI 221 CCAACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 285 TCATGA T^CATGA
SacII 618 CCGCGG CCGC^GG
TaqI 519 TCGA T^CGA
TthHB8I 519 TCGA T^CGA
Van91I 221 CCAACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 203 CCACCGAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1398 GGATCC [GA]^GATC[CT]
XmnI 993 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AocI 631 CCTGAGG CC^T[ACGT]AGG
700 CCTGAGG
BalI 68 TGGCCA TGG^CCA
200 TGGCCA
BlpI 899 GCTCAGC GC^T[ACGT]AGC
1802 GCTCAGC
Bpu1102I 899 GCTCAGC GC^T[ACGT]AGC
1802 GCTCAGC
BsmBI 1455 GAGACG CGTCTC(1/5)
1677 GAGACG
BsrFI 524 GCCGGC [GA]^CCGG[CT]
623 GCCGGT
BssSI 1695 CACGAG CACGAG(-5/-1)
1824 CACGAG
Bsu36I 631 CCTGAGG CC^T[ACGT]AGG
700 CCTGAGG
CelII 899 GCTCAGC GC^T[ACGT]AGC
1802 GCTCAGC
Cfr10I 524 GCCGGC [GA]^CCGG[CT]
623 GCCGGT
Eco81I 631 CCTGAGG CC^T[ACGT]AGG
700 CCTGAGG
EcoNI 631 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
684 CCTCTGCAAGG
EcoT14I 127 CCAAGG C^C[AT][AT]GG
212 CCTTGG
HgaI 431 GACGC GACGC(5/10)
1449 GCGTC
MaeIII 1086 GTCAC ^GT[ACGT]AC
1205 GTCAC
MluNI 68 TGGCCA TGG^CCA
200 TGGCCA
MscI 68 TGGCCA TGG^CCA
200 TGGCCA
PshAI 431 GACGCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1457 GACGTCGGTC
SmlI 597 CTCAAG C^T[CT][GA]AG
945 CTTGAG
StyI 127 CCAAGG C^C[AT][AT]GG
212 CCTTGG
TfiI 985 GATTC G^A[AT]TC
1041 GAATC
Tsp45I 1086 GTCAC ^GT[GC]AC
1205 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI TspRI