(Circular) (Six-base) MAPSORT of: pFN21AA1853.seq Check: 9788 from: 1 to: 6538 pFN21AA1853 6538 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3466 4348 278 Size: 53 83 186 936 1930 882 2468 Fragments arranged by size: 2468 1930 936 882 186 83 53 AccI GT'mk_AC Cuts at: 1937 3881 1937 Size: 1944 4594 Acc65I G'GTAC_C Cuts at: 3859 3859 Size: 6538 AclI AA'CG_TT Cuts at: 1563 4665 5038 1563 Size: 3102 373 3063 Fragments arranged by size: 3102 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4601 5985 6253 1632 Size: 2969 1384 268 1917 Fragments arranged by size: 2969 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6341 AflIII A'CryG_T Cuts at: 1203 2874 5437 6414 1203 Size: 1671 2563 977 1327 Fragments arranged by size: 2563 1671 1327 977 AgeI A'CCGG_T Cuts at: 5369 5369 Size: 6538 AhdI GACnn_n'nnGTC Cuts at: 5267 5267 Size: 6538 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6538 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2144 1184 Size: 960 5578 AlwNI CAG_nnn'CTG Cuts at: 2203 4281 5853 2203 Size: 2078 1572 2888 Fragments arranged by size: 2888 2078 1572 ApaLI G'TGCA_C Cuts at: 4595 5751 4595 Size: 1156 5382 ApoI r'AATT_y Cuts at: 1493 3847 4073 4225 6334 1493 Size: 2354 226 152 2109 1697 Fragments arranged by size: 2354 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5092 160 Size: 872 4060 1606 Fragments arranged by size: 4060 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6538 AvaI C'yCGr_G Cuts at: 1870 1943 2087 2916 3505 3863 1870 Size: 73 144 829 589 358 4545 Fragments arranged by size: 4545 829 589 358 144 73 BamHI G'GATC_C Cuts at: 3403 3868 4235 3403 Size: 465 367 5706 Fragments arranged by size: 5706 465 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2222 2264 2619 3859 5314 Size: 325 217 627 435 42 355 1240 1455 Cuts at: 5314 618 Size: 1842 Fragments arranged by size: 1842 1455 1240 627 435 355 325 217 42 BanII G_rGCy'C Cuts at: 1391 2145 2217 2588 2939 2970 3155 3335 3740 Size: 754 72 371 351 31 185 180 405 Cuts at: 3740 3857 1391 Size: 117 4072 Fragments arranged by size: 4072 754 405 371 351 185 180 117 72 31 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5567 BbvCI CC'TCA_GC Cuts at: 3593 3593 Size: 6538 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2083 2117 4729 4763 1903 Size: 34 146 34 2612 34 3678 Fragments arranged by size: 3678 2612 146 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4430 5640 4430 Size: 1210 5328 BclI T'GATC_A Cuts at: 1548 1548 Size: 6538 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5149 136 Size: 107 122 71 631 605 3477 1525 Fragments arranged by size: 3477 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6533 6533 Size: 6538 BlpI GC'TnA_GC Cuts at: 2905 3808 3955 2905 Size: 903 147 5488 Fragments arranged by size: 5488 903 147 Bme1580I G_kGCm'C Cuts at: 1790 2225 3541 4599 5755 1790 Size: 435 1316 1058 1156 2573 Fragments arranged by size: 2573 1316 1156 1058 435 BmgBI CAC'GTC Cuts at: 1318 2877 1318 Size: 1559 4979 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2270 2707 2724 Size: 416 53 83 399 24 853 437 17 Cuts at: 2724 2790 2934 4269 5227 442 Size: 66 144 1335 958 1753 Fragments arranged by size: 1753 1335 958 853 437 416 399 144 83 66 53 24 17 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6538 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2878 3067 3442 2878 Size: 189 375 5974 Fragments arranged by size: 5974 375 189 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2305 2726 3190 3536 3682 3808 5198 1239 Size: 1066 421 464 346 146 126 1390 2579 Fragments arranged by size: 2579 1390 1066 464 421 346 146 126 Bpu10I CC'TnA_GC Cuts at: 2169 3593 2169 Size: 1424 5114 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2587 2971 4663 5528 5826 2587 Size: 384 1692 865 298 3299 Fragments arranged by size: 3299 1692 865 384 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2919 5201 882 Size: 617 1420 2282 2219 Fragments arranged by size: 2282 2219 1420 617 BsaAI yAC'GTr Cuts at: 493 1204 4329 493 Size: 711 3125 2702 Fragments arranged by size: 3125 2702 711 BsaBI GATnn'nnATC Cuts at: 6532 6532 Size: 6538 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3463 4345 4727 5362 Size: 53 83 186 936 1930 882 382 635 Cuts at: 5362 275 Size: 1451 Fragments arranged by size: 1930 1451 936 882 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4971 5369 5643 5790 6201 4971 Size: 398 274 147 411 5308 Fragments arranged by size: 5308 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2926 2956 3649 3679 3806 Size: 30 165 30 1523 30 693 30 127 Cuts at: 3806 3836 1178 Size: 30 3880 Fragments arranged by size: 3880 1523 693 165 127 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2461 3004 3172 3343 3568 3595 1186 Size: 1275 543 168 171 225 27 4129 Fragments arranged by size: 4129 1275 543 225 171 168 27 BseYI C'CCAG_C Cuts at: 2164 2191 3302 3338 3389 3569 5741 6237 2164 Size: 27 1111 36 51 180 2172 496 2465 Fragments arranged by size: 2465 2172 1111 496 180 51 36 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5965 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2629 3988 4749 5777 1540 Size: 275 186 628 1359 761 1028 2301 Fragments arranged by size: 2301 1359 1028 761 628 275 186 BsiHKAI G_wGCw'C Cuts at: 2588 2970 3155 4599 4684 5755 2588 Size: 382 185 1444 85 1071 3371 Fragments arranged by size: 3371 1444 1071 382 185 85 BsmI GAATG_Cn' Cuts at: 4049 4142 4049 Size: 93 6445 BsmBI CGTCTCn'nnnn_ Cuts at: 3454 3676 3454 Size: 222 6316 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2217 2225 2588 2939 2970 3155 Size: 399 355 72 8 363 351 31 185 Cuts at: 3155 3335 3541 3740 3857 4599 4684 5755 1391 Size: 180 206 199 117 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 363 355 351 206 199 185 180 117 85 72 31 8 BspEI T'CCGG_A Cuts at: 6201 6201 Size: 6538 BspHI T'CATG_A Cuts at: 2290 4427 6157 2290 Size: 2137 1730 2671 Fragments arranged by size: 2671 2137 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2430 3893 844 Size: 1586 1463 3489 Fragments arranged by size: 3489 1586 1463 BsrBI CCG'CTC Cuts at: 1394 1823 2827 3297 3400 3633 4425 1394 Size: 429 1004 470 103 233 792 3507 Fragments arranged by size: 3507 1004 792 470 429 233 103 BsrDI GCAATG_nn' Cuts at: 65 1248 5033 5207 65 Size: 1183 3785 174 1396 Fragments arranged by size: 3785 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2529 2628 5182 5369 1700 Size: 110 719 99 2554 187 2869 Fragments arranged by size: 2869 2554 719 187 110 99 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6538 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6538 BssSI C'ACGA_G Cuts at: 3700 3829 4598 5610 3700 Size: 129 769 1012 4628 Fragments arranged by size: 4628 1012 769 129 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6538 BstBI TT'CG_AA Cuts at: 5342 5342 Size: 6538 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6538 BstXI CCAn_nnnn'nTGG Cuts at: 2750 2750 Size: 6538 BstYI r'GATC_y Cuts at: 1972 3403 3868 4235 4620 4637 6089 6175 6187 Size: 1431 465 367 385 17 1452 86 12 Cuts at: 6187 6533 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1431 465 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2637 2706 2637 Size: 69 6469 BtgI C'CryG_G Cuts at: 513 1065 2004 2623 513 Size: 552 939 619 4428 Fragments arranged by size: 4428 939 619 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2373 487 Size: 683 56 1147 4652 Fragments arranged by size: 4652 1147 683 56 BtsI GCAGTG_nn' Cuts at: 4054 4848 4868 4054 Size: 794 20 5724 Fragments arranged by size: 5724 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6538 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6503 DraI TTT'AAA Cuts at: 3842 3842 Size: 6538 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5545 809 Size: 507 4229 1802 Fragments arranged by size: 4229 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 2205 2626 Size: 54 1425 50 275 18 243 132 421 Cuts at: 2626 3636 3985 4874 6503 8 Size: 1010 349 889 1629 43 Fragments arranged by size: 1629 1425 1010 889 421 349 275 243 132 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2626 3985 1537 Size: 275 814 1359 4090 Fragments arranged by size: 4090 1359 814 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2354 2450 2488 2498 3277 4468 1353 Size: 1001 96 38 10 779 1191 3423 Fragments arranged by size: 3423 1191 1001 779 96 38 10 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5125 5499 5645 387 Size: 1064 138 3536 374 146 1280 Fragments arranged by size: 3536 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2586 2968 3153 2586 Size: 382 185 5971 Fragments arranged by size: 5971 382 185 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2305 2726 3190 3536 3682 3808 4601 Size: 393 673 421 464 346 146 126 793 Cuts at: 4601 5198 5985 6253 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 793 787 673 597 464 421 393 346 268 146 126 EcoNI CCTnn'n_nnAGG Cuts at: 2640 2693 2640 Size: 53 6485 EcoO109I rG'GnC_Cy Cuts at: 2370 3285 3354 2370 Size: 915 69 5554 Fragments arranged by size: 5554 915 69 EcoRI G'AATT_C Cuts at: 3847 4225 3847 Size: 378 6160 FspI TGC'GCA Cuts at: 5044 5044 Size: 6538 HaeII r_GCGC'y Cuts at: 1678 3493 5685 1678 Size: 1815 2192 2531 Fragments arranged by size: 2531 2192 1815 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2165 2197 2699 2731 2864 Size: 32 135 32 593 32 502 32 133 Cuts at: 2864 2896 3649 3681 3806 3838 5179 5211 5253 Size: 32 753 32 125 32 1341 32 42 Cuts at: 5253 5285 1373 Size: 32 2626 Fragments arranged by size: 2626 1341 753 593 502 135 133 125 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2735 3882 4128 669 Size: 988 281 797 1147 246 3079 Fragments arranged by size: 3079 1147 988 797 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6538 HpaI GTT'AAC Cuts at: 4128 4128 Size: 6538 KpnI G_GTAC'C Cuts at: 3863 3863 Size: 6538 MfeI C'AATT_G Cuts at: 4137 4137 Size: 6538 MluI A'CGCG_T Cuts at: 6414 6414 Size: 6538 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3021 3437 3647 5652 5836 1305 Size: 1716 416 210 2005 184 2007 Fragments arranged by size: 2007 2005 1716 416 210 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2075 2207 10 Size: 54 1425 343 243 132 4341 Fragments arranged by size: 4341 1425 343 243 132 54 MslI CAynn'nnrTG Cuts at: 518 1166 4496 4855 5014 518 Size: 648 3330 359 159 2042 Fragments arranged by size: 3330 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2128 2565 2625 2676 3436 3954 4631 5779 Size: 522 437 60 51 760 518 677 1148 Cuts at: 5779 6024 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 760 677 522 518 437 245 60 51 NaeI GCC'GGC Cuts at: 2531 2531 Size: 6538 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5047 Fragments arranged by size: 5047 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6538 NgoMIV G'CCGG_C Cuts at: 2529 2529 Size: 6538 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6538 NotI GC'GGCC_GC Cuts at: 3985 3985 Size: 6538 NruI TCG'CGA Cuts at: 2839 2839 Size: 6538 NspI r_CATG'y Cuts at: 1132 1229 3896 5441 1132 Size: 97 2667 1545 2229 Fragments arranged by size: 2667 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3234 1348 Size: 1886 4652 PciI A'CATG_T Cuts at: 5437 5437 Size: 6538 PflMI CCAn_nnn'nTGG Cuts at: 1108 2232 1108 Size: 1124 5414 PfoI T'CCnGG_A Cuts at: 3653 3653 Size: 6538 PmeI GTTT'AAAC Cuts at: 3842 3842 Size: 6538 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4607 4639 6120 6152 4607 Size: 32 1481 32 4993 Fragments arranged by size: 4993 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2370 3285 3354 2370 Size: 915 69 5554 Fragments arranged by size: 5554 915 69 PshAI GACnn'nnGTC Cuts at: 1613 2440 3466 1613 Size: 827 1026 4685 Fragments arranged by size: 4685 1026 827 PsiI TTA'TAA Cuts at: 4108 4108 Size: 6538 PstI C_TGCA'G Cuts at: 830 3890 830 Size: 3060 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6538 SacI G_AGCT'C Cuts at: 2588 2970 3155 2588 Size: 382 185 5971 Fragments arranged by size: 5971 382 185 SacII CC_GC'GG Cuts at: 2626 2626 Size: 6538 SalI G'TCGA_C Cuts at: 1936 3880 1936 Size: 1944 4594 SbfI CC_TGCA'GG Cuts at: 3890 3890 Size: 6538 ScaI AGT'ACT Cuts at: 4786 6498 4786 Size: 1712 4826 SfcI C'TryA_G Cuts at: 826 1045 2380 3382 3802 3886 5021 5702 5893 Size: 219 1335 1002 420 84 1135 681 191 Cuts at: 5893 826 Size: 1471 Fragments arranged by size: 1471 1335 1135 1002 681 420 219 191 84 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6538 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6538 SmaI CCC'GGG Cuts at: 1872 3865 1872 Size: 1993 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2602 2950 4642 5543 5805 820 Size: 197 926 659 348 1692 901 262 1553 Fragments arranged by size: 1692 1553 926 901 659 348 262 197 SnaBI TAC'GTA Cuts at: 493 4329 493 Size: 3836 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6538 SphI G_CATG'C Cuts at: 1132 3896 1132 Size: 2764 3774 SspI AAT'ATT Cuts at: 5 52 4462 5 Size: 47 4410 2081 Fragments arranged by size: 4410 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6538 StyI C'CwwG_G Cuts at: 513 1065 2004 2132 2217 3977 513 Size: 552 939 128 85 1760 3074 Fragments arranged by size: 3074 1760 939 552 128 85 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2252 3172 3269 3456 4565 4582 4735 Size: 1255 672 920 97 187 1109 17 153 Cuts at: 4735 4920 5365 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1109 920 672 445 187 185 153 97 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4784 6496 96 Size: 274 80 33 51 1443 2807 1712 138 Fragments arranged by size: 2807 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2068 2100 2926 2958 3520 3552 6498 6530 2068 Size: 32 826 32 562 32 2946 32 2076 Fragments arranged by size: 2946 2076 826 562 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6538 XbaI T'CTAG_A Cuts at: 3874 3874 Size: 6538 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2215 2215 Size: 6538 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6538 XmaI C'CCGG_G Cuts at: 1870 3863 1870 Size: 1993 4545 XmnI GAAnn'nnTTC Cuts at: 3002 4667 3002 Size: 1665 4873 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3464 4346 276 Size: 53 83 186 936 1930 882 2468 Fragments arranged by size: 2468 1930 936 882 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI