Restriction map for
pFN21AA1853
(Linear) (Six-base) MAPSORT of: KIAA1853.seq Check: 1058 from: 1 to: 1833
KIAA1853.orf
With 182 enzymes: SgfI *
November 13, 2006 11:21 ..
AatII G_ACGT'C
Cuts at: 0 1461 1833
Size: 1461 372
AflIII A'CryG_T
Cuts at: 0 869 1833
Size: 869 964
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 139 1833
Size: 139 1694
AlwNI CAG_nnn'CTG
Cuts at: 0 198 1833
Size: 198 1635
AvaI C'yCGr_G
Cuts at: 0 82 911 1500 1833
Size: 82 829 589 333
Fragments arranged by size:
829 589 333 82
BamHI G'GATC_C
Cuts at: 0 1398 1833
Size: 1398 435
BanI G'GyrC_C
Cuts at: 0 217 259 614 1833
Size: 217 42 355 1219
Fragments arranged by size:
1219 355 217 42
BanII G_rGCy'C
Cuts at: 0 140 212 583 934 965 1150 1330 1735
Size: 140 72 371 351 31 185 180 405
Cuts at: 1735 1833
Size: 98
Fragments arranged by size:
405 371 351 185 180 140 98 72
31
BbvCI CC'TCA_GC
Cuts at: 0 1588 1833
Size: 1588 245
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 78 112 1833
Size: 78 34 1721
Fragments arranged by size:
1721 78 34
BlpI GC'TnA_GC
Cuts at: 0 900 1803 1833
Size: 900 903 30
Fragments arranged by size:
903 900 30
Bme1580I G_kGCm'C
Cuts at: 0 220 1536 1833
Size: 220 1316 297
Fragments arranged by size:
1316 297 220
BmgBI CAC'GTC
Cuts at: 0 872 1833
Size: 872 961
BmrI ACTGGGnnnn_n'
Cuts at: 0 265 702 719 785 929 1833
Size: 265 437 17 66 144 904
Fragments arranged by size:
904 437 265 144 66 17
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 873 1062 1437 1833
Size: 873 189 375 396
Fragments arranged by size:
873 396 375 189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 721 1185 1531 1677 1803 1833
Size: 300 421 464 346 146 126 30
Fragments arranged by size:
464 421 346 300 146 126 30
Bpu10I CC'TnA_GC
Cuts at: 0 164 1588 1833
Size: 164 1424 245
Fragments arranged by size:
1424 245 164
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 582 966 1833
Size: 582 384 867
Fragments arranged by size:
867 582 384
BsaI GGTCTCn'nnnn_
Cuts at: 0 914 1833
Size: 914 919
BsaHI Gr'CG_yC
Cuts at: 0 1458 1833
Size: 1458 375
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 921 951 1644 1674 1801 1831 1833
Size: 921 30 693 30 127 30 2
Fragments arranged by size:
921 693 127 30 30 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 456 999 1167 1338 1563 1590 1833
Size: 456 543 168 171 225 27 243
Fragments arranged by size:
543 456 243 225 171 168 27
BseYI C'CCAG_C
Cuts at: 0 159 186 1297 1333 1384 1564 1833
Size: 159 27 1111 36 51 180 269
Fragments arranged by size:
1111 269 180 159 51 36 27
BsiEI CG_ry'CG
Cuts at: 0 624 1833
Size: 624 1209
BsiHKAI G_wGCw'C
Cuts at: 0 583 965 1150 1833
Size: 583 382 185 683
Fragments arranged by size:
683 583 382 185
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1449 1671 1833
Size: 1449 222 162
Fragments arranged by size:
1449 222 162
Bsp1286I G_dGCh'C
Cuts at: 0 140 212 220 583 934 965 1150 1330
Size: 140 72 8 363 351 31 185 180
Cuts at: 1330 1536 1735 1833
Size: 206 199 98
Fragments arranged by size:
363 351 206 199 185 180 140 98
72 31 8
BspHI T'CATG_A
Cuts at: 0 285 1833
Size: 285 1548
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 425 1833
Size: 425 1408
BsrBI CCG'CTC
Cuts at: 0 822 1292 1395 1628 1833
Size: 822 470 103 233 205
Fragments arranged by size:
822 470 233 205 103
BsrFI r'CCGG_y
Cuts at: 0 524 623 1833
Size: 524 99 1210
Fragments arranged by size:
1210 524 99
BssSI C'ACGA_G
Cuts at: 0 1695 1824 1833
Size: 1695 129 9
Fragments arranged by size:
1695 129 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 745 1833
Size: 745 1088
BstYI r'GATC_y
Cuts at: 0 1398 1833
Size: 1398 435
Bsu36I CC'TnA_GG
Cuts at: 0 632 701 1833
Size: 632 69 1132
Fragments arranged by size:
1132 632 69
BtgI C'CryG_G
Cuts at: 0 618 1833
Size: 618 1215
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 368 1833
Size: 368 1465
EaeI y'GGCC_r
Cuts at: 0 68 200 621 1631 1833
Size: 68 132 421 1010 202
Fragments arranged by size:
1010 421 202 132 68
EagI C'GGCC_G
Cuts at: 0 621 1833
Size: 621 1212
EarI CTCTTCn'nnn_
Cuts at: 0 349 445 483 493 1272 1833
Size: 349 96 38 10 779 561
Fragments arranged by size:
779 561 349 96 38 10
EcoICRI GAG'CTC
Cuts at: 0 581 963 1148 1833
Size: 581 382 185 685
Fragments arranged by size:
685 581 382 185
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 721 1185 1531 1677 1803 1833
Size: 300 421 464 346 146 126 30
Fragments arranged by size:
464 421 346 300 146 126 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 635 688 1833
Size: 635 53 1145
Fragments arranged by size:
1145 635 53
EcoO109I rG'GnC_Cy
Cuts at: 0 365 1280 1349 1833
Size: 365 915 69 484
Fragments arranged by size:
915 484 365 69
HaeII r_GCGC'y
Cuts at: 0 1488 1833
Size: 1488 345
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 160 192 694 726 859 891 1644 1676
Size: 160 32 502 32 133 32 753 32
Cuts at: 1676 1801 1833
Size: 125 32
Fragments arranged by size:
753 502 160 133 125 32 32 32
32 32
HincII GTy'rAC
Cuts at: 0 730 1833
Size: 730 1103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1016 1432 1642 1833
Size: 1016 416 210 191
Fragments arranged by size:
1016 416 210 191
MscI TGG'CCA
Cuts at: 0 70 202 1833
Size: 70 132 1631
Fragments arranged by size:
1631 132 70
MspA1I CmG'CkG
Cuts at: 0 123 560 620 671 1431 1833
Size: 123 437 60 51 760 402
Fragments arranged by size:
760 437 402 123 60 51
NaeI GCC'GGC
Cuts at: 0 526 1833
Size: 526 1307
NgoMIV G'CCGG_C
Cuts at: 0 524 1833
Size: 524 1309
NruI TCG'CGA
Cuts at: 0 834 1833
Size: 834 999
PasI CC'CwG_GG
Cuts at: 0 1229 1833
Size: 1229 604
PflMI CCAn_nnn'nTGG
Cuts at: 0 227 1833
Size: 227 1606
PfoI T'CCnGG_A
Cuts at: 0 1648 1833
Size: 1648 185
PpuMI rG'GwC_Cy
Cuts at: 0 365 1280 1349 1833
Size: 365 915 69 484
Fragments arranged by size:
915 484 365 69
PshAI GACnn'nnGTC
Cuts at: 0 435 1461 1833
Size: 435 1026 372
Fragments arranged by size:
1026 435 372
SacI G_AGCT'C
Cuts at: 0 583 965 1150 1833
Size: 583 382 185 683
Fragments arranged by size:
683 583 382 185
SacII CC_GC'GG
Cuts at: 0 621 1833
Size: 621 1212
SfcI C'TryA_G
Cuts at: 0 375 1377 1797 1833
Size: 375 1002 420 36
Fragments arranged by size:
1002 420 375 36
SmlI C'TyrA_G
Cuts at: 0 597 945 1833
Size: 597 348 888
Fragments arranged by size:
888 597 348
StyI C'CwwG_G
Cuts at: 0 127 212 1833
Size: 127 85 1621
Fragments arranged by size:
1621 127 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 247 1167 1264 1451 1833
Size: 247 920 97 187 382
Fragments arranged by size:
920 382 247 187 97
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 63 95 921 953 1515 1547 1833
Size: 63 32 826 32 562 32 286
Fragments arranged by size:
826 562 286 63 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 210 1833
Size: 210 1623
XmnI GAAnn'nnTTC
Cuts at: 0 997 1833
Size: 997 836
ZraI GAC'GTC
Cuts at: 0 1459 1833
Size: 1459 374
Enzymes that do cut:
AatII AflIII AlfI AlwNI AvaI BamHI BanI BanII
BbvCI BcgI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BssSI
BstXI BstYI Bsu36I BtgI BtgZI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII MmeI
MscI MspA1I NaeI NgoMIV NruI PasI PflMI PfoI
PpuMI PshAI SacI SacII SfcI SmlI StyI TaqII
TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BbsI BciVI BclI BfrBI
BglI BglII BmtI BsaAI BsaBI BsaWI BsgI BsiWI
BsmI BspEI BsrDI BsrGI BssHII BstAPI BstBI BstEII
BstZ17I BtsI ClaI CspCI DraI DraIII DrdI EciI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI MslI NarI NcoI NdeI
NheI NotI NsiI NspI PacI PciI PmeI PmlI
PpiI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI Tth111I XbaI XhoI XmaI