(Circular) (Six-base) MAPSORT of: pFN21AA1851.seq Check: 4505 from: 1 to: 6217 pFN21AA1851 6217 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2541 2565 2700 2541 Size: 24 135 6058 Fragments arranged by size: 6058 135 24 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4027 278 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3560 1937 Size: 1623 4594 Acc65I G'GTAC_C Cuts at: 2770 3538 2770 Size: 768 5449 AclI AA'CG_TT Cuts at: 1563 4344 4717 1563 Size: 2781 373 3063 Fragments arranged by size: 3063 2781 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3482 4280 5664 5932 1632 Size: 1850 798 1384 268 1917 Fragments arranged by size: 1917 1850 1384 798 268 AfeI AGC'GCT Cuts at: 2702 2702 Size: 6217 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6020 AflIII A'CryG_T Cuts at: 1203 2361 5116 6093 1203 Size: 1158 2755 977 1327 Fragments arranged by size: 2755 1327 1158 977 AgeI A'CCGG_T Cuts at: 5048 5048 Size: 6217 AhdI GACnn_n'nnGTC Cuts at: 4946 4946 Size: 6217 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6217 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3011 1184 Size: 1827 4390 AlwNI CAG_nnn'CTG Cuts at: 2230 3422 3960 5532 2230 Size: 1192 538 1572 2915 Fragments arranged by size: 2915 1572 1192 538 ApaI G_GGCC'C Cuts at: 3075 3075 Size: 6217 ApaLI G'TGCA_C Cuts at: 2441 3116 4274 5430 2441 Size: 675 1158 1156 3228 Fragments arranged by size: 3228 1158 1156 675 ApoI r'AATT_y Cuts at: 1493 3428 3526 3752 3904 6013 1493 Size: 1935 98 226 152 2109 1697 Fragments arranged by size: 2109 1935 1697 226 152 98 AseI AT'TA_AT Cuts at: 160 1032 4771 160 Size: 872 3739 1606 Fragments arranged by size: 3739 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 AvaI C'yCGr_G Cuts at: 1870 1943 3137 3542 1870 Size: 73 1194 405 4545 Fragments arranged by size: 4545 1194 405 73 AvrII C'CTAG_G Cuts at: 2152 2152 Size: 6217 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2101 2134 2762 2795 2101 Size: 33 628 33 5523 Fragments arranged by size: 5523 628 33 33 BamHI G'GATC_C Cuts at: 2972 3547 3914 2972 Size: 575 367 5275 Fragments arranged by size: 5275 575 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2060 2248 2706 2770 2965 Size: 325 217 627 273 188 458 64 195 Cuts at: 2965 3538 4993 618 Size: 573 1455 1842 Fragments arranged by size: 1842 1455 627 573 458 325 273 217 195 188 64 BanII G_rGCy'C Cuts at: 1391 3075 3138 3536 1391 Size: 1684 63 398 4072 Fragments arranged by size: 4072 1684 398 63 BbeI G_GCGC'C Cuts at: 2252 2969 2252 Size: 717 5500 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2308 928 Size: 971 409 4837 Fragments arranged by size: 4837 971 409 BbvCI CC'TCA_GC Cuts at: 3423 3423 Size: 6217 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2165 2199 2279 2313 2491 2525 4408 Size: 34 228 34 80 34 178 34 1883 Cuts at: 4408 4442 1903 Size: 34 3678 Fragments arranged by size: 3678 1883 228 178 80 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4109 5319 4109 Size: 1210 5007 BclI T'GATC_A Cuts at: 1548 1548 Size: 6217 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3390 3399 4828 Size: 107 122 71 631 605 1718 9 1429 Cuts at: 4828 136 Size: 1525 Fragments arranged by size: 1718 1525 1429 631 605 122 107 71 9 BglII A'GATC_T Cuts at: 6212 6212 Size: 6217 BlpI GC'TnA_GC Cuts at: 2138 2480 3290 3634 2138 Size: 342 810 344 4721 Fragments arranged by size: 4721 810 344 342 Bme1580I G_kGCm'C Cuts at: 1790 2445 3075 3120 4278 5434 1790 Size: 655 630 45 1158 1156 2573 Fragments arranged by size: 2573 1158 1156 655 630 45 BmgBI CAC'GTC Cuts at: 1318 2167 1318 Size: 849 5368 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3078 3948 4906 Size: 416 53 83 399 24 1661 870 958 Cuts at: 4906 442 Size: 1753 Fragments arranged by size: 1753 1661 958 870 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6217 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2839 2839 Size: 6217 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 2429 3395 3468 4877 1239 Size: 968 222 966 73 1409 2579 Fragments arranged by size: 2579 1409 968 966 222 73 Bpu10I CC'TnA_GC Cuts at: 2207 2607 2720 3423 2207 Size: 400 113 703 5001 Fragments arranged by size: 5001 703 400 113 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2618 4342 5207 5505 2618 Size: 1724 865 298 3330 Fragments arranged by size: 3330 1724 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4880 882 Size: 617 3381 2219 Fragments arranged by size: 3381 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2752 3364 4008 493 Size: 711 1548 612 644 2702 Fragments arranged by size: 2702 1548 711 644 612 BsaBI GATnn'nnATC Cuts at: 6211 6211 Size: 6217 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2249 2313 2966 4024 Size: 53 83 186 936 716 64 653 1058 Cuts at: 4024 4406 5041 275 Size: 382 635 1451 Fragments arranged by size: 1451 1058 936 716 653 635 382 186 83 64 53 BsaWI w'CCGG_w Cuts at: 4650 5048 5322 5469 5880 4650 Size: 398 274 147 411 4987 Fragments arranged by size: 4987 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2561 2591 1178 Size: 30 165 30 1158 30 4804 Fragments arranged by size: 4804 1158 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2566 1186 Size: 1380 4837 BseYI C'CCAG_C Cuts at: 2023 2302 2398 2587 2833 3169 3272 3335 5420 Size: 279 96 189 246 336 103 63 2085 Cuts at: 5420 5916 2023 Size: 496 2324 Fragments arranged by size: 2324 2085 496 336 279 246 189 103 96 63 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2128 2258 2519 3065 3428 1163 Size: 573 392 130 261 546 363 3952 Fragments arranged by size: 3952 573 546 392 363 261 130 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2855 3667 4428 5456 1540 Size: 275 186 854 812 761 1028 2301 Fragments arranged by size: 2301 1028 854 812 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2445 2865 3120 3195 4278 4363 5434 2445 Size: 420 255 75 1083 85 1071 3228 Fragments arranged by size: 3228 1083 1071 420 255 85 75 BsmI GAATG_Cn' Cuts at: 3728 3821 3728 Size: 93 6124 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2445 2865 3075 3120 3138 3195 3536 Size: 399 655 420 210 45 18 57 341 Cuts at: 3536 4278 4363 5434 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 655 420 399 341 210 85 57 45 18 BspEI T'CCGG_A Cuts at: 5880 5880 Size: 6217 BspHI T'CATG_A Cuts at: 4106 5836 4106 Size: 1730 4487 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2142 2191 2541 2565 2700 3572 844 Size: 1298 49 350 24 135 872 3489 Fragments arranged by size: 3489 1298 872 350 135 49 24 BsrBI CCG'CTC Cuts at: 1394 1823 2851 3280 4104 1394 Size: 429 1028 429 824 3507 Fragments arranged by size: 3507 1028 824 429 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2928 4712 4886 65 Size: 1183 1680 1784 174 1396 Fragments arranged by size: 1784 1680 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2583 2623 4861 5048 1700 Size: 110 773 40 2238 187 2869 Fragments arranged by size: 2869 2238 773 187 110 40 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6217 BssHII G'CGCG_C Cuts at: 1925 2266 2473 2475 2741 1925 Size: 341 207 2 266 5401 Fragments arranged by size: 5401 341 266 207 2 BssSI C'ACGA_G Cuts at: 3098 3194 4277 5289 3098 Size: 96 1083 1012 4026 Fragments arranged by size: 4026 1083 1012 96 BstAPI GCAn_nnn'nTGC Cuts at: 794 2167 794 Size: 1373 4844 BstBI TT'CG_AA Cuts at: 5021 5021 Size: 6217 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6217 BstXI CCAn_nnnn'nTGG Cuts at: 2587 3169 2587 Size: 582 5635 BstYI r'GATC_y Cuts at: 1972 2972 3547 3914 4299 4316 5768 5854 5866 Size: 1000 575 367 385 17 1452 86 12 Cuts at: 5866 6212 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1000 575 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2336 2891 2336 Size: 555 5662 BtgI C'CryG_G Cuts at: 513 1065 2464 3180 513 Size: 552 1399 716 3550 Fragments arranged by size: 3550 1399 716 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2974 2994 487 Size: 683 56 1748 20 3710 Fragments arranged by size: 3710 1748 683 56 20 BtsI GCAGTG_nn' Cuts at: 2030 3164 3733 4527 4547 2030 Size: 1134 569 794 20 3700 Fragments arranged by size: 3700 1134 794 569 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6217 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6182 DraI TTT'AAA Cuts at: 3521 3521 Size: 6217 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5224 809 Size: 507 3908 1802 Fragments arranged by size: 3908 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2377 2613 2852 Size: 54 1425 50 275 18 547 236 239 Cuts at: 2852 2919 3000 3015 3063 3400 3664 4553 6182 Size: 67 81 15 48 337 264 889 1629 Cuts at: 6182 8 Size: 43 Fragments arranged by size: 1629 1425 889 547 337 275 264 239 236 81 67 54 50 48 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2852 3664 1537 Size: 275 1040 812 4090 Fragments arranged by size: 4090 1040 812 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2261 2853 3056 4147 1353 Size: 908 592 203 1091 3423 Fragments arranged by size: 3423 1091 908 592 203 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3469 4804 5178 5324 387 Size: 1064 138 1880 1335 374 146 1280 Fragments arranged by size: 1880 1335 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2429 3395 3468 3482 4280 4877 Size: 393 575 222 966 73 14 798 597 Cuts at: 4877 5664 5932 1239 Size: 787 268 1524 Fragments arranged by size: 1524 966 798 787 597 575 393 268 222 73 14 EcoO109I rG'GnC_Cy Cuts at: 2716 3072 3391 3503 2716 Size: 356 319 112 5430 Fragments arranged by size: 5430 356 319 112 EcoRI G'AATT_C Cuts at: 3428 3526 3904 3428 Size: 98 378 5741 Fragments arranged by size: 5741 378 98 FspI TGC'GCA Cuts at: 2927 4723 2927 Size: 1796 4421 FspAI rTGC'GCAy Cuts at: 2927 2927 Size: 6217 HaeII r_GCGC'y Cuts at: 1678 2230 2252 2375 2408 2550 2704 2727 2969 Size: 552 22 123 33 142 154 23 242 Cuts at: 2969 5364 1678 Size: 2395 2531 Fragments arranged by size: 2531 2395 552 242 154 142 123 33 23 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2339 2371 2561 2593 4858 Size: 32 135 32 767 32 190 32 2265 Cuts at: 4858 4890 4932 4964 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2265 767 190 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2323 3561 3807 669 Size: 988 281 385 1238 246 3079 Fragments arranged by size: 3079 1238 988 385 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6217 HpaI GTT'AAC Cuts at: 3807 3807 Size: 6217 KasI G'GCGC_C Cuts at: 2248 2965 2248 Size: 717 5500 KpnI G_GTAC'C Cuts at: 2774 3542 2774 Size: 768 5449 MfeI C'AATT_G Cuts at: 3816 3816 Size: 6217 MluI A'CGCG_T Cuts at: 6093 6093 Size: 6217 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2604 5331 5515 1305 Size: 1299 2727 184 2007 Fragments arranged by size: 2727 2007 1299 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3017 3065 10 Size: 54 1425 343 1185 48 3162 Fragments arranged by size: 3162 1425 1185 343 54 48 MslI CAynn'nnrTG Cuts at: 518 1166 2993 4175 4534 4693 518 Size: 648 1827 1182 359 159 2042 Fragments arranged by size: 2042 1827 1182 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2263 2331 2713 3228 3249 3258 3412 3460 Size: 657 68 382 515 21 9 154 48 Cuts at: 3460 3633 4310 5458 5703 1606 Size: 173 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 657 515 382 245 173 154 68 48 21 9 NaeI GCC'GGC Cuts at: 2625 2625 Size: 6217 NarI GG'CG_CC Cuts at: 2249 2966 2249 Size: 717 5500 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5665 NdeI CA'TA_TG Cuts at: 387 387 Size: 6217 NgoMIV G'CCGG_C Cuts at: 2623 2623 Size: 6217 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6217 NotI GC'GGCC_GC Cuts at: 3664 3664 Size: 6217 NspI r_CATG'y Cuts at: 1132 1229 2044 2459 3044 3575 5120 1132 Size: 97 815 415 585 531 1545 2229 Fragments arranged by size: 2229 1545 815 585 531 415 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6217 PciI A'CATG_T Cuts at: 5116 5116 Size: 6217 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2840 1108 Size: 902 830 4485 Fragments arranged by size: 4485 902 830 PmeI GTTT'AAAC Cuts at: 3521 3521 Size: 6217 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4286 4318 5799 5831 4286 Size: 32 1481 32 4672 Fragments arranged by size: 4672 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3503 3503 Size: 6217 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6217 PsiI TTA'TAA Cuts at: 3787 3787 Size: 6217 PspOMI G'GGCC_C Cuts at: 3071 3071 Size: 6217 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2630 2662 2630 Size: 32 6185 PstI C_TGCA'G Cuts at: 830 2163 3459 3569 830 Size: 1333 1296 110 3478 Fragments arranged by size: 3478 1333 1296 110 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6217 PvuII CAG'CTG Cuts at: 2263 3412 3460 2263 Size: 1149 48 5020 Fragments arranged by size: 5020 1149 48 SalI G'TCGA_C Cuts at: 1936 3559 1936 Size: 1623 4594 SanDI GG'GwC_CC Cuts at: 3503 3503 Size: 6217 SapI GCTCTTCn'nnn_ Cuts at: 2853 2853 Size: 6217 SbfI CC_TGCA'GG Cuts at: 3569 3569 Size: 6217 ScaI AGT'ACT Cuts at: 3309 4465 6177 3309 Size: 1156 1712 3349 Fragments arranged by size: 3349 1712 1156 SexAI A'CCwGG_T Cuts at: 2009 2009 Size: 6217 SfcI C'TryA_G Cuts at: 826 1045 2159 2696 3455 3565 4700 5381 5572 Size: 219 1114 537 759 110 1135 681 191 Cuts at: 5572 826 Size: 1471 Fragments arranged by size: 1471 1135 1114 759 681 537 219 191 110 SfiI GGCCn_nnn'nGGCC Cuts at: 3390 3399 3390 Size: 9 6208 SfoI GGC'GCC Cuts at: 2250 2967 2250 Size: 717 5500 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6217 SmaI CCC'GGG Cuts at: 1872 3544 1872 Size: 1672 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2633 4321 5222 5484 820 Size: 197 926 690 1688 901 262 1553 Fragments arranged by size: 1688 1553 926 901 690 262 197 SnaBI TAC'GTA Cuts at: 493 2752 4008 493 Size: 2259 1256 2702 Fragments arranged by size: 2702 2259 1256 SpeI A'CTAG_T Cuts at: 152 152 Size: 6217 SphI G_CATG'C Cuts at: 1132 2044 2459 3044 3575 1132 Size: 912 415 585 531 3774 Fragments arranged by size: 3774 912 585 531 415 SspI AAT'ATT Cuts at: 5 52 4141 5 Size: 47 4089 2081 Fragments arranged by size: 4089 2081 47 StuI AGG'CCT Cuts at: 1517 3453 1517 Size: 1936 4281 StyI C'CwwG_G Cuts at: 513 1065 2152 3656 513 Size: 552 1087 1504 3074 Fragments arranged by size: 3074 1504 1087 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2567 2874 3286 3349 4244 4261 4414 Size: 1255 987 307 412 63 895 17 153 Cuts at: 4414 4599 5044 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 987 895 445 412 307 185 153 63 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3102 3307 4463 Size: 274 80 33 51 1443 1125 205 1156 Cuts at: 4463 6175 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1156 1125 274 205 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3039 3071 6177 6209 3039 Size: 32 3106 32 3047 Fragments arranged by size: 3106 3047 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6217 XbaI T'CTAG_A Cuts at: 3553 3553 Size: 6217 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6217 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6217 XmaI C'CCGG_G Cuts at: 1870 3542 1870 Size: 1672 4545 XmnI GAAnn'nnTTC Cuts at: 4346 4346 Size: 6217 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4025 276 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI NruI NsiI PacI PfoI PmlI PspXI RsrII SacI SacII SrfI SwaI