(Circular) (Six-base) MAPSORT of: pFN21AA1847.seq Check: 7020 from: 1 to: 6238 pFN21AA1847 6238 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2050 2339 2593 2050 Size: 289 254 5695 Fragments arranged by size: 5695 289 254 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4048 278 Size: 53 83 186 936 2512 2468 Fragments arranged by size: 2512 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3581 1937 Size: 1644 4594 Acc65I G'GTAC_C Cuts at: 3559 3559 Size: 6238 AclI AA'CG_TT Cuts at: 1563 4365 4738 1563 Size: 2802 373 3063 Fragments arranged by size: 3063 2802 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2153 2555 4301 5685 5953 1632 Size: 521 402 1746 1384 268 1917 Fragments arranged by size: 1917 1746 1384 521 402 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6041 AflIII A'CryG_T Cuts at: 1203 3273 5137 6114 1203 Size: 2070 1864 977 1327 Fragments arranged by size: 2070 1864 1327 977 AgeI A'CCGG_T Cuts at: 5069 5069 Size: 6238 AhdI GACnn_n'nnGTC Cuts at: 4967 4967 Size: 6238 AleI CACnn'nnGTG Cuts at: 1166 2723 1166 Size: 1557 4681 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6238 AlwNI CAG_nnn'CTG Cuts at: 2342 2427 2891 3981 5553 2342 Size: 85 464 1090 1572 3027 Fragments arranged by size: 3027 1572 1090 464 85 ApaI G_GGCC'C Cuts at: 3232 3307 3232 Size: 75 6163 ApaLI G'TGCA_C Cuts at: 2936 4295 5451 2936 Size: 1359 1156 3723 Fragments arranged by size: 3723 1359 1156 ApoI r'AATT_y Cuts at: 1493 3547 3773 3925 6034 1493 Size: 2054 226 152 2109 1697 Fragments arranged by size: 2109 2054 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4792 160 Size: 872 3760 1606 Fragments arranged by size: 3760 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 AvaI C'yCGr_G Cuts at: 1870 1943 2215 3051 3563 1870 Size: 73 272 836 512 4545 Fragments arranged by size: 4545 836 512 272 73 BamHI G'GATC_C Cuts at: 3568 3935 3568 Size: 367 5871 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2078 2278 3116 3135 3559 Size: 325 217 627 291 200 838 19 424 Cuts at: 3559 5014 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 838 627 424 325 291 217 200 19 BanII G_rGCy'C Cuts at: 1391 2142 2151 2654 3030 3232 3307 3448 3475 Size: 751 9 503 376 202 75 141 27 Cuts at: 3475 3557 1391 Size: 82 4072 Fragments arranged by size: 4072 751 503 376 202 141 82 75 27 9 BbeI G_GCGC'C Cuts at: 2082 2082 Size: 6238 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5267 BbvCI CC'TCA_GC Cuts at: 2768 2768 Size: 6238 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4429 4463 1903 Size: 34 2492 34 3678 Fragments arranged by size: 3678 2492 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4130 5340 4130 Size: 1210 5028 BclI T'GATC_A Cuts at: 1548 1548 Size: 6238 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3245 3302 4849 Size: 107 122 71 631 605 1573 57 1547 Cuts at: 4849 136 Size: 1525 Fragments arranged by size: 1573 1547 1525 631 605 122 107 71 57 BglII A'GATC_T Cuts at: 6233 6233 Size: 6238 BlpI GC'TnA_GC Cuts at: 3655 3655 Size: 6238 Bme1580I G_kGCm'C Cuts at: 1790 2435 2546 2940 3232 3307 4299 5455 1790 Size: 645 111 394 292 75 992 1156 2573 Fragments arranged by size: 2573 1156 992 645 394 292 111 75 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6238 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3969 4927 442 Size: 416 53 83 399 24 2552 958 1753 Fragments arranged by size: 2552 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6238 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2503 2851 2503 Size: 348 5890 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2042 2459 2576 2845 2941 4898 1239 Size: 803 417 117 269 96 1957 2579 Fragments arranged by size: 2579 1957 803 417 269 117 96 Bpu10I CC'TnA_GC Cuts at: 2648 2768 3308 3474 2648 Size: 120 540 166 5412 Fragments arranged by size: 5412 540 166 120 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4363 5228 5526 4363 Size: 865 298 5075 Fragments arranged by size: 5075 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2303 2614 3074 4901 882 Size: 617 804 311 460 1827 2219 Fragments arranged by size: 2219 1827 804 617 460 311 BsaAI yAC'GTr Cuts at: 493 1204 4029 493 Size: 711 2825 2702 Fragments arranged by size: 2825 2702 711 BsaBI GATnn'nnATC Cuts at: 6232 6232 Size: 6238 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2079 3413 4045 4427 Size: 53 83 186 936 546 1334 632 382 Cuts at: 4427 5062 275 Size: 635 1451 Fragments arranged by size: 1451 1334 936 635 632 546 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2300 4671 5069 5343 5490 5901 2300 Size: 2371 398 274 147 411 2637 Fragments arranged by size: 2637 2371 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2408 2438 2549 2579 2636 Size: 30 165 30 1005 30 111 30 57 Cuts at: 2636 2666 1178 Size: 30 4750 Fragments arranged by size: 4750 1005 165 111 57 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 2375 2954 3165 3274 3277 3347 1186 Size: 841 348 579 211 109 3 70 4077 Fragments arranged by size: 4077 841 579 348 211 109 70 3 BseYI C'CCAG_C Cuts at: 2928 3169 3250 3351 3420 3447 3457 5441 5937 Size: 241 81 101 69 27 10 1984 496 Cuts at: 5937 2928 Size: 3229 Fragments arranged by size: 3229 1984 496 241 101 81 69 27 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5665 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3688 4449 5477 1540 Size: 275 186 1687 761 1028 2301 Fragments arranged by size: 2301 1687 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2142 2151 2654 2940 4299 4384 5455 2142 Size: 9 503 286 1359 85 1071 2925 Fragments arranged by size: 2925 1359 1071 503 286 85 9 BsmI GAATG_Cn' Cuts at: 3749 3842 3749 Size: 93 6145 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2151 2435 2546 2654 2940 3030 Size: 399 352 9 284 111 108 286 90 Cuts at: 3030 3232 3307 3448 3475 3557 4299 4384 5455 Size: 202 75 141 27 82 742 85 1071 Cuts at: 5455 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 352 286 284 202 141 111 108 90 85 82 75 27 9 BspEI T'CCGG_A Cuts at: 2300 5901 2300 Size: 3601 2637 BspHI T'CATG_A Cuts at: 4127 5857 4127 Size: 1730 4508 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2050 2118 2175 2339 2577 2593 2915 3593 Size: 1206 68 57 164 238 16 322 678 Cuts at: 3593 844 Size: 3489 Fragments arranged by size: 3489 1206 678 322 238 164 68 57 16 BsrBI CCG'CTC Cuts at: 1394 1823 4125 1394 Size: 429 2302 3507 Fragments arranged by size: 3507 2302 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4733 4907 65 Size: 1183 3485 174 1396 Fragments arranged by size: 3485 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2081 2663 3399 3482 4882 5069 1700 Size: 110 271 582 736 83 1400 187 2869 Fragments arranged by size: 2869 1400 736 582 271 187 110 83 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6238 BssHII G'CGCG_C Cuts at: 1925 2409 1925 Size: 484 5754 BssSI C'ACGA_G Cuts at: 4298 5310 4298 Size: 1012 5226 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6238 BstBI TT'CG_AA Cuts at: 5042 5042 Size: 6238 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6238 BstXI CCAn_nnnn'nTGG Cuts at: 2461 2878 2461 Size: 417 5821 BstYI r'GATC_y Cuts at: 1972 3568 3935 4320 4337 5789 5875 5887 6233 Size: 1596 367 385 17 1452 86 12 346 Cuts at: 6233 1972 Size: 1977 Fragments arranged by size: 1977 1596 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2293 2454 2596 2778 513 Size: 552 939 289 161 142 182 3973 Fragments arranged by size: 3973 939 552 289 182 161 142 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5499 Fragments arranged by size: 5499 683 56 BtsI GCAGTG_nn' Cuts at: 2807 3754 4548 4568 2807 Size: 947 794 20 4477 Fragments arranged by size: 4477 947 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6238 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6203 DraI TTT'AAA Cuts at: 3542 3542 Size: 6238 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5245 809 Size: 507 3929 1802 Fragments arranged by size: 3929 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2193 2679 3397 Size: 54 1425 50 275 18 363 486 718 Cuts at: 3397 3685 4574 6203 8 Size: 288 889 1629 43 Fragments arranged by size: 1629 1425 889 718 486 363 288 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3685 1537 Size: 275 1873 4090 Fragments arranged by size: 4090 1873 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2174 2202 2361 2379 2466 3139 3326 4168 Size: 821 28 159 18 87 673 187 842 Cuts at: 4168 1353 Size: 3423 Fragments arranged by size: 3423 842 821 673 187 159 87 28 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2492 4825 5199 5345 387 Size: 1064 138 903 2333 374 146 1280 Fragments arranged by size: 2333 1280 1064 903 374 146 138 EcoICRI GAG'CTC Cuts at: 2140 2149 2652 2140 Size: 9 503 5726 Fragments arranged by size: 5726 503 9 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2153 2459 2555 2576 2845 2941 Size: 393 410 111 306 96 21 269 96 Cuts at: 2941 4301 4898 5685 5953 1239 Size: 1360 597 787 268 1524 Fragments arranged by size: 1524 1360 787 597 410 393 306 269 268 111 96 96 21 EcoNI CCTnn'n_nnAGG Cuts at: 3491 3491 Size: 6238 EcoO109I rG'GnC_Cy Cuts at: 2128 2638 2904 3228 3229 3304 2128 Size: 510 266 324 1 75 5062 Fragments arranged by size: 5062 510 324 266 75 1 EcoRI G'AATT_C Cuts at: 3547 3925 3547 Size: 378 5860 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2587 2587 Size: 6238 FspI TGC'GCA Cuts at: 2049 2813 4744 2049 Size: 764 1931 3543 Fragments arranged by size: 3543 1931 764 HaeII r_GCGC'y Cuts at: 1678 2082 2215 3454 5385 1678 Size: 404 133 1239 1931 2531 Fragments arranged by size: 2531 1931 1239 404 133 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2636 2668 4879 4911 4953 Size: 32 135 32 1064 32 2211 32 42 Cuts at: 4953 4985 1373 Size: 32 2626 Fragments arranged by size: 2626 2211 1064 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3582 3828 669 Size: 988 281 1644 246 3079 Fragments arranged by size: 3079 1644 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6238 HpaI GTT'AAC Cuts at: 3828 3828 Size: 6238 KasI G'GCGC_C Cuts at: 2078 2078 Size: 6238 KpnI G_GTAC'C Cuts at: 3563 3563 Size: 6238 MfeI C'AATT_G Cuts at: 3837 3837 Size: 6238 MluI A'CGCG_T Cuts at: 6114 6114 Size: 6238 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2856 5352 5536 1305 Size: 1551 2496 184 2007 Fragments arranged by size: 2496 2007 1551 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2681 10 Size: 54 1425 343 849 3567 Fragments arranged by size: 3567 1425 849 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2459 2691 2723 2972 4196 4555 4714 Size: 648 1293 232 32 249 1224 359 159 Cuts at: 4714 518 Size: 2042 Fragments arranged by size: 2042 1293 1224 648 359 249 232 159 32 MspA1I CmG'CkG Cuts at: 1606 2053 2119 2288 3254 3355 3424 3457 3654 Size: 447 66 169 966 101 69 33 197 Cuts at: 3654 4331 5479 5724 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 966 677 447 245 197 169 101 69 66 33 NaeI GCC'GGC Cuts at: 2083 2665 3401 3484 2083 Size: 582 736 83 4837 Fragments arranged by size: 4837 736 582 83 NarI GG'CG_CC Cuts at: 2079 2079 Size: 6238 NcoI C'CATG_G Cuts at: 513 1065 2004 2454 2596 513 Size: 552 939 450 142 4155 Fragments arranged by size: 4155 939 552 450 142 NdeI CA'TA_TG Cuts at: 387 387 Size: 6238 NgoMIV G'CCGG_C Cuts at: 2081 2663 3399 3482 2081 Size: 582 736 83 4837 Fragments arranged by size: 4837 736 582 83 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6238 NotI GC'GGCC_GC Cuts at: 3685 3685 Size: 6238 NspI r_CATG'y Cuts at: 1132 1229 3277 3596 5141 1132 Size: 97 2048 319 1545 2229 Fragments arranged by size: 2229 2048 1545 319 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6238 PciI A'CATG_T Cuts at: 3273 5137 3273 Size: 1864 4374 PflMI CCAn_nnn'nTGG Cuts at: 1108 3040 3076 1108 Size: 1932 36 4270 Fragments arranged by size: 4270 1932 36 PfoI T'CCnGG_A Cuts at: 2250 2553 2634 3372 2250 Size: 303 81 738 5116 Fragments arranged by size: 5116 738 303 81 PmeI GTTT'AAAC Cuts at: 3542 3542 Size: 6238 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4307 4339 5820 5852 4307 Size: 32 1481 32 4693 Fragments arranged by size: 4693 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2128 2638 2904 2128 Size: 510 266 5462 Fragments arranged by size: 5462 510 266 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6238 PsiI TTA'TAA Cuts at: 3808 3808 Size: 6238 PspOMI G'GGCC_C Cuts at: 3228 3303 3228 Size: 75 6163 PspXI vC'TCGA_Gb Cuts at: 3051 3051 Size: 6238 PstI C_TGCA'G Cuts at: 830 2058 2124 2588 2661 2912 3090 3429 3492 Size: 1228 66 464 73 251 178 339 63 Cuts at: 3492 3590 830 Size: 98 3478 Fragments arranged by size: 3478 1228 464 339 251 178 98 73 66 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6238 PvuII CAG'CTG Cuts at: 2053 2119 3424 3457 2053 Size: 66 1305 33 4834 Fragments arranged by size: 4834 1305 66 33 SacI G_AGCT'C Cuts at: 2142 2151 2654 2142 Size: 9 503 5726 Fragments arranged by size: 5726 503 9 SalI G'TCGA_C Cuts at: 1936 3580 1936 Size: 1644 4594 SapI GCTCTTCn'nnn_ Cuts at: 2174 2202 2379 3326 2174 Size: 28 177 947 5086 Fragments arranged by size: 5086 947 177 28 SbfI CC_TGCA'GG Cuts at: 2661 3590 2661 Size: 929 5309 ScaI AGT'ACT Cuts at: 4486 6198 4486 Size: 1712 4526 SfcI C'TryA_G Cuts at: 826 1045 2054 2120 2419 2584 2657 2908 3086 Size: 219 1009 66 299 165 73 251 178 Cuts at: 3086 3425 3488 3586 4721 5402 5593 826 Size: 339 63 98 1135 681 191 1471 Fragments arranged by size: 1471 1135 1009 681 339 299 251 219 191 178 165 98 73 66 63 SfoI GGC'GCC Cuts at: 2080 2080 Size: 6238 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6238 SmaI CCC'GGG Cuts at: 1872 2217 3565 1872 Size: 345 1348 4545 Fragments arranged by size: 4545 1348 345 SmlI C'TyrA_G Cuts at: 820 1017 1943 3051 4342 5243 5505 820 Size: 197 926 1108 1291 901 262 1553 Fragments arranged by size: 1553 1291 1108 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4029 493 Size: 3536 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6238 SphI G_CATG'C Cuts at: 1132 3596 1132 Size: 2464 3774 SrfI GCCC'GGGC Cuts at: 2217 2217 Size: 6238 SspI AAT'ATT Cuts at: 5 52 4162 5 Size: 47 4110 2081 Fragments arranged by size: 4110 2081 47 StuI AGG'CCT Cuts at: 1517 3157 1517 Size: 1640 4598 StyI C'CwwG_G Cuts at: 513 1065 2004 2073 2454 2596 3677 513 Size: 552 939 69 381 142 1081 3074 Fragments arranged by size: 3074 1081 939 552 381 142 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3530 4265 4282 4435 4620 5065 325 Size: 1255 1950 735 17 153 185 445 1498 Fragments arranged by size: 1950 1498 1255 735 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4484 6196 96 Size: 274 80 33 51 1443 2507 1712 138 Fragments arranged by size: 2507 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2549 2581 6198 6230 2549 Size: 32 3617 32 2557 Fragments arranged by size: 3617 2557 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6238 XbaI T'CTAG_A Cuts at: 3574 3574 Size: 6238 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3037 3037 Size: 6238 XhoI C'TCGA_G Cuts at: 1943 3051 1943 Size: 1108 5130 XmaI C'CCGG_G Cuts at: 1870 2215 3563 1870 Size: 345 1348 4545 Fragments arranged by size: 4545 1348 345 XmnI GAAnn'nnTTC Cuts at: 4367 4367 Size: 6238 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4046 276 Size: 53 83 186 936 2512 2468 Fragments arranged by size: 2512 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PsrI RsrII SacII SanDI SexAI SfiI SwaI