Restriction map for
pFN21AA1838
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# NO CUT ENZYMES ( 101 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AvaI
AvrII BamHI BbeI BbvCI BcgI
BclI BfrI BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BssHII BstBI
BstEII BstPI CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoT22I FbaI FseI HpaI
KasI KpnI MamI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmeI PpuMI
PshBI Psp1406I PvuI RsrII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccII 2332 CGCG CG^CG
AcyI 1000 GACGCC G[GA]^CG[CT]C
AflIII 1171 ACGTGT A^C[GA][CT]GT
AhdI 1877 GACAGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 401 CCTCAGG CC^T[ACGT]AGG
Asp700I 1015 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1877 GACAGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2229 TGCGCA TGC^GCA
BalI 183 TGGCCA TGG^CCA
BanI 1976 GGCACC G^G[CT][GA]CC
BbrPI 1170 CACGTG CAC^GTG
BcnI 1980 CCCGG CC^[GC]GG
BglI 680 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2491 AGATCT A^GATCT
BsaAI 1170 CACGTG [CT]AC^GT[GA]
BsaHI 1000 GACGCC G[GA]^CG[CT]C
BsaI 2678 GAGACC GGTCTC(1/5)
BseRI 2112 CTCCTC GAGGAG(10/8)
BsgI 2308 GTGCAG GTGCAG(16/14)
BsmI 1804 GAATGC GAATGC(1/-1)
BspHI 1784 TCATGA T^CATGA
BssSI 990 CACGAG CACGAG(-5/-1)
BstAPI 2534 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 2332 CGCG CG^CG
Bsu36I 401 CCTCAGG CC^T[ACGT]AGG
Eam1105I 1877 GACAGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 401 CCTCAGG CC^T[ACGT]AGG
EcoRV 333 GATATC GAT^ATC
FauI 1881 GCGGG CCCGC(4/6)
FspI 2229 TGCGCA TGC^GCA
HaeII 2075 GGCGCT [GA]GCGC^[CT]
HapII 1981 CCGG C^CGG
Hin1I 1000 GACGCC G[GA]^CG[CT]C
HincII 2356 GTCAAC GT[CT]^[GA]AC
HindII 2356 GTCAAC GT[CT]^[GA]AC
HpaII 1981 CCGG C^CGG
KspI 2331 CCGCGG CCGC^GG
MluNI 183 TGGCCA TGG^CCA
MscI 183 TGGCCA TGG^CCA
MspI 1981 CCGG C^CGG
MvnI 2332 CGCG CG^CG
NciI 1980 CCCGG CC^[GC]GG
NdeI 1527 CATATG CA^TATG
NheI 408 GCTAGC G^CTAGC
PmaCI 1170 CACGTG CAC^GTG
PmlI 1170 CACGTG CAC^GTG
PshAI 556 GACACTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1784 TCATGA T^CATGA
SacI 583 GAGCTC GAGCT^C
SacII 2331 CCGCGG CCGC^GG
SapI 2122 GAAGAGC GCTCTTC(1/4)
ScaI 2438 AGTACT AGT^ACT
SexAI 1769 ACCTGGT A^CC[AT]GGT
SphI 2217 GCATGC GCATG^C
SspI 2037 AATATT AAT^ATT
XmnI 1015 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AspHI 82 GAGCAC G[AT]GC[AT]^C
583 GAGCTC
AspI 604 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1984 GACCTCGTC
BciVI 99 GTATCC GTATCC(6/5)
1956 GTATCC
BsiHKAI 82 GAGCAC G[AT]GC[AT]^C
583 GAGCTC
BsmAI 2678 GAGAC GTCTC(1/5)
143 GTCTC
BspMI 37 ACCTGC ACCTGC(4/8)
1927 ACCTGC
BstF5I 396 CATCC GGATG(2/0)
106 GGATG
BstXI 163 CCAGAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1670 CCACAAATGTGG
FokI 396 CATCC GGATG(9/13)
106 GGATG
HgaI 1000 GACGC GACGC(5/10)
2558 GACGC
MaeII 1171 ACGT A^CGT
1751 ACGT
NcoI 934 CCATGG C^CATGG
1706 CCATGG
PflFI 604 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1984 GACCTCGTC
PvuII 239 CAGCTG CAG^CTG
2311 CAGCTG
SmlI 316 CTCAAG C^T[CT][GA]AG
2115 CTCAAG
Tsp45I 360 GTCAC ^GT[GC]AC
1918 GTCAC
Tth111I 604 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1984 GACCTCGTC
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaII BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BsiYI BslI BsmFI
Bsp1286I Bsp1407I BsrGI BsrI BssKI
Bst1107I BstNI BstYI BstZ17I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI HaeIII HhaI HinP1I
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII