(Circular) (Six-base) MAPSORT of: pFN21AA1838.seq Check: 5920 from: 1 to: 7435 pFN21AA1838 7435 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3941 3941 Size: 7435 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5245 278 Size: 53 83 186 936 3709 2468 Fragments arranged by size: 3709 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2162 3107 3287 3323 3359 3395 3431 3891 Size: 225 945 180 36 36 36 36 460 Cuts at: 3891 4778 1937 Size: 887 4594 Fragments arranged by size: 4594 945 887 460 225 180 36 36 36 36 Acc65I G'GTAC_C Cuts at: 4756 4756 Size: 7435 AclI AA'CG_TT Cuts at: 1563 5562 5935 1563 Size: 3999 373 3063 Fragments arranged by size: 3999 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3282 3711 4544 5498 6882 7150 1632 Size: 1650 429 833 954 1384 268 1917 Fragments arranged by size: 1917 1650 1384 954 833 429 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7238 AflIII A'CryG_T Cuts at: 1203 3176 6334 7311 1203 Size: 1973 3158 977 1327 Fragments arranged by size: 3158 1973 1327 977 AgeI A'CCGG_T Cuts at: 6266 6266 Size: 7435 AhdI GACnn_n'nnGTC Cuts at: 3887 6164 3887 Size: 2277 5158 AleI CACnn'nnGTG Cuts at: 1166 3680 1166 Size: 2514 4921 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4552 1184 Size: 3368 4067 AlwNI CAG_nnn'CTG Cuts at: 3044 3080 3116 3152 3188 3224 3260 3296 3332 Size: 36 36 36 36 36 36 36 36 Cuts at: 3332 3368 3404 3440 3476 3725 3933 5178 6750 Size: 36 36 36 36 249 208 1245 1572 Cuts at: 6750 3044 Size: 3729 Fragments arranged by size: 3729 1572 1245 249 208 36 36 36 36 36 36 36 36 36 36 36 36 ApaLI G'TGCA_C Cuts at: 5492 6648 5492 Size: 1156 6279 ApoI r'AATT_y Cuts at: 1493 2068 3620 4511 4744 4970 5122 7231 1493 Size: 575 1552 891 233 226 152 2109 1697 Fragments arranged by size: 2109 1697 1552 891 575 233 226 152 AseI AT'TA_AT Cuts at: 160 1032 5989 160 Size: 872 4957 1606 Fragments arranged by size: 4957 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7435 AvaI C'yCGr_G Cuts at: 1870 1943 4760 1870 Size: 73 2817 4545 Fragments arranged by size: 4545 2817 73 BamHI G'GATC_C Cuts at: 4765 5132 4765 Size: 367 7068 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3981 4756 6211 618 Size: 325 217 627 2194 775 1455 1842 Fragments arranged by size: 2194 1842 1455 775 627 325 217 BanII G_rGCy'C Cuts at: 1391 2592 4325 4582 4592 4625 4646 4754 1391 Size: 1201 1733 257 10 33 21 108 4072 Fragments arranged by size: 4072 1733 1201 257 108 33 21 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2544 3567 3675 4394 4621 928 Size: 971 645 1023 108 719 227 3742 Fragments arranged by size: 3742 1023 971 719 645 227 108 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5626 5660 1903 Size: 34 3689 34 3678 Fragments arranged by size: 3689 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2115 3972 5327 6537 2115 Size: 1857 1355 1210 3013 Fragments arranged by size: 3013 1857 1355 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7435 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2691 6046 136 Size: 107 122 71 631 605 1019 3355 1525 Fragments arranged by size: 3355 1525 1019 631 605 122 107 71 BglII A'GATC_T Cuts at: 4496 7430 4496 Size: 2934 4501 BlpI GC'TnA_GC Cuts at: 4852 4852 Size: 7435 Bme1580I G_kGCm'C Cuts at: 1790 5496 6652 1790 Size: 3706 1156 2573 Fragments arranged by size: 3706 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3757 1318 Size: 2439 4996 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2456 3914 4594 Size: 416 53 83 399 24 1039 1458 680 Cuts at: 4594 5166 6124 442 Size: 572 958 1753 Fragments arranged by size: 1753 1458 1039 958 680 572 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2417 1056 Size: 1361 6074 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2651 2651 Size: 7435 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2151 2492 4232 4609 6095 1239 Size: 912 341 1740 377 1486 2579 Fragments arranged by size: 2579 1740 1486 912 377 341 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2306 4105 5560 6425 6723 2306 Size: 1799 1455 865 298 3018 Fragments arranged by size: 3018 1799 1455 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4677 6098 882 Size: 617 3178 1421 2219 Fragments arranged by size: 3178 2219 1421 617 BsaAI yAC'GTr Cuts at: 493 1204 3177 5226 493 Size: 711 1973 2049 2702 Fragments arranged by size: 2702 2049 1973 711 BsaBI GATnn'nnATC Cuts at: 7429 7429 Size: 7435 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3006 5242 5624 6259 Size: 53 83 186 936 1473 2236 382 635 Cuts at: 6259 275 Size: 1451 Fragments arranged by size: 2236 1473 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5868 6266 6540 6687 7098 5868 Size: 398 274 147 411 6205 Fragments arranged by size: 6205 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7210 Fragments arranged by size: 7210 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4108 1186 Size: 2922 4513 BseYI C'CCAG_C Cuts at: 2246 3851 4318 6638 7134 2246 Size: 1605 467 2320 496 2547 Fragments arranged by size: 2547 2320 1605 496 467 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4334 1163 Size: 573 2598 4264 Fragments arranged by size: 4264 2598 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4885 5646 6674 1540 Size: 275 186 2884 761 1028 2301 Fragments arranged by size: 2884 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2091 2592 5496 5581 6652 2091 Size: 501 2904 85 1071 2874 Fragments arranged by size: 2904 2874 1071 501 85 BsmI GAATG_Cn' Cuts at: 3815 4946 5039 3815 Size: 1131 93 6211 Fragments arranged by size: 6211 1131 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2592 4325 4582 4592 4625 4646 Size: 399 301 501 1733 257 10 33 21 Cuts at: 4646 4754 5496 5581 6652 1391 Size: 108 742 85 1071 2174 Fragments arranged by size: 2174 1733 1071 742 501 399 301 257 108 85 33 21 10 BspEI T'CCGG_A Cuts at: 7098 7098 Size: 7435 BspHI T'CATG_A Cuts at: 3789 5324 7054 3789 Size: 1535 1730 4170 Fragments arranged by size: 4170 1730 1535 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2051 3941 4790 844 Size: 1207 1890 849 3489 Fragments arranged by size: 3489 1890 1207 849 BsrBI CCG'CTC Cuts at: 1394 1823 5322 1394 Size: 429 3499 3507 Fragments arranged by size: 3507 3499 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5930 6104 65 Size: 1183 4682 174 1396 Fragments arranged by size: 4682 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6079 6266 1700 Size: 110 4269 187 2869 Fragments arranged by size: 4269 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2057 3071 3179 3251 3500 3536 3572 3644 Size: 1961 1014 108 72 249 36 36 72 Cuts at: 3644 3780 96 Size: 136 3751 Fragments arranged by size: 3751 1961 1014 249 136 108 72 72 36 36 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7435 BssSI C'ACGA_G Cuts at: 2995 5495 6507 2995 Size: 2500 1012 3923 Fragments arranged by size: 3923 2500 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4545 794 Size: 3751 3684 BstBI TT'CG_AA Cuts at: 6239 6239 Size: 7435 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7435 BstXI CCAn_nnnn'nTGG Cuts at: 2175 3682 2175 Size: 1507 5928 BstYI r'GATC_y Cuts at: 1972 2178 3541 4496 4765 5132 5517 5534 6986 Size: 206 1363 955 269 367 385 17 1452 Cuts at: 6986 7072 7084 7430 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1363 955 385 367 346 269 206 86 17 12 BstZ17I GTA'TAC Cuts at: 2163 3108 3288 3324 3360 3396 3432 2163 Size: 945 180 36 36 36 36 6166 Fragments arranged by size: 6166 945 180 36 36 36 36 Bsu36I CC'TnA_GG Cuts at: 2407 2407 Size: 7435 BtgI C'CryG_G Cuts at: 513 1065 2004 2939 3711 4336 4676 513 Size: 552 939 935 772 625 340 3272 Fragments arranged by size: 3272 939 935 772 625 552 340 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6696 Fragments arranged by size: 6696 683 56 BtsI GCAGTG_nn' Cuts at: 3802 4951 5745 5765 3802 Size: 1149 794 20 5472 Fragments arranged by size: 5472 1149 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7435 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7400 DraI TTT'AAA Cuts at: 4739 4739 Size: 7435 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6442 809 Size: 507 5126 1802 Fragments arranged by size: 5126 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2188 2661 4573 Size: 54 1425 50 275 18 358 473 1912 Cuts at: 4573 4882 5771 7400 8 Size: 309 889 1629 43 Fragments arranged by size: 1912 1629 1425 889 473 358 309 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4882 1537 Size: 275 3070 4090 Fragments arranged by size: 4090 3070 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2679 2853 4122 5365 1353 Size: 1326 174 1269 1243 3423 Fragments arranged by size: 3423 1326 1269 1243 174 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6022 6396 6542 387 Size: 1064 138 4433 374 146 1280 Fragments arranged by size: 4433 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2590 2590 Size: 7435 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2151 2492 3282 3711 4232 4544 4609 Size: 393 519 341 790 429 521 312 65 Cuts at: 4609 5498 6095 6882 7150 1239 Size: 889 597 787 268 1524 Fragments arranged by size: 1524 889 790 787 597 521 519 429 393 341 312 268 65 EcoO109I rG'GnC_Cy Cuts at: 3077 3185 3257 3401 3437 3686 3077 Size: 108 72 144 36 249 6826 Fragments arranged by size: 6826 249 144 108 72 36 EcoRI G'AATT_C Cuts at: 4744 5122 4744 Size: 378 7057 EcoRV GAT'ATC Cuts at: 2340 2340 Size: 7435 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3851 3851 Size: 7435 FspI TGC'GCA Cuts at: 4236 5941 4236 Size: 1705 5730 HaeII r_GCGC'y Cuts at: 1678 4084 6582 1678 Size: 2406 2498 2531 Fragments arranged by size: 2531 2498 2406 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2801 2833 2854 2886 2996 Size: 32 135 32 1229 32 21 32 110 Cuts at: 2996 3028 3032 3064 3464 3496 3533 3565 3873 Size: 32 4 32 400 32 37 32 308 Cuts at: 3873 3905 4488 4520 6076 6108 6150 6182 1373 Size: 32 583 32 1556 32 42 32 2626 Fragments arranged by size: 2626 1556 1229 583 400 308 135 110 42 37 32 32 32 32 32 32 32 32 32 32 32 32 21 4 HincII GTy'rAC Cuts at: 669 1657 1938 4363 4779 5025 669 Size: 988 281 2425 416 246 3079 Fragments arranged by size: 3079 2425 988 416 281 246 HindIII A'AGCT_T Cuts at: 748 2725 4158 4427 748 Size: 1977 1433 269 3756 Fragments arranged by size: 3756 1977 1433 269 HpaI GTT'AAC Cuts at: 5025 5025 Size: 7435 KpnI G_GTAC'C Cuts at: 4760 4760 Size: 7435 MfeI C'AATT_G Cuts at: 5034 5034 Size: 7435 MluI A'CGCG_T Cuts at: 7311 7311 Size: 7435 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6549 6733 1305 Size: 5244 184 2007 Fragments arranged by size: 5244 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2190 10 Size: 54 1425 343 358 5255 Fragments arranged by size: 5255 1425 358 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2053 2615 3680 5393 5752 5911 Size: 648 843 44 562 1065 1713 359 159 Cuts at: 5911 518 Size: 2042 Fragments arranged by size: 2042 1713 1065 843 648 562 359 159 44 MspA1I CmG'CkG Cuts at: 1606 2246 3886 4003 4318 4338 4851 5528 6676 Size: 640 1640 117 315 20 513 677 1148 Cuts at: 6676 6921 1606 Size: 245 2120 Fragments arranged by size: 2120 1640 1148 677 640 513 315 245 117 20 NcoI C'CATG_G Cuts at: 513 1065 2004 2939 3711 513 Size: 552 939 935 772 4237 Fragments arranged by size: 4237 939 935 772 552 NdeI CA'TA_TG Cuts at: 387 3533 387 Size: 3146 4289 NheI G'CTAG_C Cuts at: 1052 2413 1052 Size: 1361 6074 NotI GC'GGCC_GC Cuts at: 4882 4882 Size: 7435 NspI r_CATG'y Cuts at: 1132 1229 2719 4226 4374 4793 6338 1132 Size: 97 1490 1507 148 419 1545 2229 Fragments arranged by size: 2229 1545 1507 1490 419 148 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7435 PciI A'CATG_T Cuts at: 6334 6334 Size: 7435 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7435 PfoI T'CCnGG_A Cuts at: 2107 2342 2107 Size: 235 7200 PmeI GTTT'AAAC Cuts at: 4739 4739 Size: 7435 PmlI CAC'GTG Cuts at: 3177 3177 Size: 7435 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5504 5536 7017 7049 5504 Size: 32 1481 32 5890 Fragments arranged by size: 5890 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2565 1613 Size: 952 6483 PsiI TTA'TAA Cuts at: 2898 5005 2898 Size: 2107 5328 PstI C_TGCA'G Cuts at: 830 2627 4008 4137 4787 830 Size: 1797 1381 129 650 3478 Fragments arranged by size: 3478 1797 1381 650 129 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7435 PvuII CAG'CTG Cuts at: 2246 4318 2246 Size: 2072 5363 SacI G_AGCT'C Cuts at: 2592 2592 Size: 7435 SacII CC_GC'GG Cuts at: 4339 4339 Size: 7435 SalI G'TCGA_C Cuts at: 1936 4777 1936 Size: 2841 4594 SapI GCTCTTCn'nnn_ Cuts at: 4122 4122 Size: 7435 SbfI CC_TGCA'GG Cuts at: 4787 4787 Size: 7435 ScaI AGT'ACT Cuts at: 4445 5683 7395 4445 Size: 1238 1712 4485 Fragments arranged by size: 4485 1712 1238 SexAI A'CCwGG_T Cuts at: 3774 3774 Size: 7435 SfcI C'TryA_G Cuts at: 826 1045 2623 3603 4004 4133 4283 4783 5918 Size: 219 1578 980 401 129 150 500 1135 Cuts at: 5918 6599 6790 826 Size: 681 191 1471 Fragments arranged by size: 1578 1471 1135 980 681 500 401 219 191 150 129 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7435 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7435 SmaI CCC'GGG Cuts at: 1872 4762 1872 Size: 2890 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2321 4120 5539 6440 6702 820 Size: 197 926 378 1799 1419 901 262 1553 Fragments arranged by size: 1799 1553 1419 926 901 378 262 197 SnaBI TAC'GTA Cuts at: 493 5226 493 Size: 4733 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7435 SphI G_CATG'C Cuts at: 1132 4226 4793 1132 Size: 3094 567 3774 Fragments arranged by size: 3774 3094 567 SspI AAT'ATT Cuts at: 5 52 4044 5359 5 Size: 47 3992 1315 2081 Fragments arranged by size: 3992 2081 1315 47 StuI AGG'CCT Cuts at: 1517 3845 3860 4241 4544 1517 Size: 2328 15 381 303 4408 Fragments arranged by size: 4408 2328 381 303 15 StyI C'CwwG_G Cuts at: 513 1065 2004 2689 2939 3711 4252 4874 513 Size: 552 939 685 250 772 541 622 3074 Fragments arranged by size: 3074 939 772 685 622 552 541 250 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1997 2296 5462 5479 5632 5817 6262 Size: 1255 417 299 3166 17 153 185 445 Cuts at: 6262 325 Size: 1498 Fragments arranged by size: 3166 1498 1255 445 417 299 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2057 3071 3179 Size: 274 80 33 51 1443 80 1014 108 Cuts at: 3179 3251 3500 3536 3572 3644 3780 4443 5681 Size: 72 249 36 36 72 136 663 1238 Cuts at: 5681 7393 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1238 1014 663 274 249 138 136 108 80 80 72 72 51 36 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2170 2202 2447 2479 3741 3773 4378 4410 7395 Size: 32 245 32 1262 32 605 32 2985 Cuts at: 7395 7427 2170 Size: 32 2178 Fragments arranged by size: 2985 2178 1262 605 245 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2612 3992 1316 Size: 1296 1380 4759 Fragments arranged by size: 4759 1380 1296 XbaI T'CTAG_A Cuts at: 4771 4771 Size: 7435 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7435 XmaI C'CCGG_G Cuts at: 1870 4760 1870 Size: 2890 4545 XmnI GAAnn'nnTTC Cuts at: 3024 5564 3024 Size: 2540 4895 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5243 276 Size: 53 83 186 936 3709 2468 Fragments arranged by size: 3709 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SanDI SfiI SfoI SrfI SwaI XcmI