Restriction map for
pFN21AA1838
(Linear) (Six-base) MAPSORT of: KIAA1838.seq Check: 3956 from: 1 to: 2730
KIAA1838.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1936 2730
Size: 1936 794
AccI GT'mk_AC
Cuts at: 0 157 1102 1282 1318 1354 1390 1426 1886
Size: 157 945 180 36 36 36 36 460
Cuts at: 1886 2730
Size: 844
Fragments arranged by size:
945 844 460 180 157 36 36 36
36
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1277 1706 2539 2730
Size: 1277 429 833 191
Fragments arranged by size:
1277 833 429 191
AflIII A'CryG_T
Cuts at: 0 1171 2730
Size: 1171 1559
AhdI GACnn_n'nnGTC
Cuts at: 0 1882 2730
Size: 1882 848
AleI CACnn'nnGTG
Cuts at: 0 1675 2730
Size: 1675 1055
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2547 2730
Size: 2547 183
AlwNI CAG_nnn'CTG
Cuts at: 0 1039 1075 1111 1147 1183 1219 1255 1291
Size: 1039 36 36 36 36 36 36 36
Cuts at: 1291 1327 1363 1399 1435 1471 1720 1928 2730
Size: 36 36 36 36 36 249 208 802
Fragments arranged by size:
1039 802 249 208 36 36 36 36
36 36 36 36 36 36 36 36
ApoI r'AATT_y
Cuts at: 0 63 1615 2506 2730
Size: 63 1552 891 224
Fragments arranged by size:
1552 891 224 63
BanI G'GyrC_C
Cuts at: 0 1976 2730
Size: 1976 754
BanII G_rGCy'C
Cuts at: 0 587 2320 2577 2587 2620 2641 2730
Size: 587 1733 257 10 33 21 89
Fragments arranged by size:
1733 587 257 89 33 21 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 539 1562 1670 2389 2616 2730
Size: 539 1023 108 719 227 114
Fragments arranged by size:
1023 719 539 227 114 108
BciVI GTATCCnnnnn_n'
Cuts at: 0 110 1967 2730
Size: 110 1857 763
Fragments arranged by size:
1857 763 110
BglI GCCn_nnn'nGGC
Cuts at: 0 686 2730
Size: 686 2044
BglII A'GATC_T
Cuts at: 0 2491 2730
Size: 2491 239
BmgBI CAC'GTC
Cuts at: 0 1752 2730
Size: 1752 978
BmrI ACTGGGnnnn_n'
Cuts at: 0 451 1909 2589 2730
Size: 451 1458 680 141
Fragments arranged by size:
1458 680 451 141
BmtI G_CTAG'C
Cuts at: 0 412 2730
Size: 412 2318
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 646 2730
Size: 646 2084
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 487 2227 2604 2730
Size: 146 341 1740 377 126
Fragments arranged by size:
1740 377 341 146 126
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 2100 2730
Size: 301 1799 630
Fragments arranged by size:
1799 630 301
BsaI GGTCTCn'nnnn_
Cuts at: 0 2672 2730
Size: 2672 58
BsaAI yAC'GTr
Cuts at: 0 1172 2730
Size: 1172 1558
BsaHI Gr'CG_yC
Cuts at: 0 1001 2730
Size: 1001 1729
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2103 2730
Size: 2103 627
BseYI C'CCAG_C
Cuts at: 0 241 1846 2313 2730
Size: 241 1605 467 417
Fragments arranged by size:
1605 467 417 241
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2329 2730
Size: 2329 401
BsiHKAI G_wGCw'C
Cuts at: 0 86 587 2730
Size: 86 501 2143
Fragments arranged by size:
2143 501 86
BsmI GAATG_Cn'
Cuts at: 0 1810 2730
Size: 1810 920
Bsp1286I G_dGCh'C
Cuts at: 0 86 587 2320 2577 2587 2620 2641 2730
Size: 86 501 1733 257 10 33 21 89
Fragments arranged by size:
1733 501 257 89 86 33 21 10
BspHI T'CATG_A
Cuts at: 0 1784 2730
Size: 1784 946
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 46 1936 2730
Size: 46 1890 794
Fragments arranged by size:
1890 794 46
BsrGI T'GTAC_A
Cuts at: 0 52 1066 1174 1246 1495 1531 1567 1639
Size: 52 1014 108 72 249 36 36 72
Cuts at: 1639 1775 2730
Size: 136 955
Fragments arranged by size:
1014 955 249 136 108 72 72 52
36 36
BssSI C'ACGA_G
Cuts at: 0 990 2730
Size: 990 1740
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2540 2730
Size: 2540 190
BstXI CCAn_nnnn'nTGG
Cuts at: 0 170 1677 2730
Size: 170 1507 1053
Fragments arranged by size:
1507 1053 170
BstYI r'GATC_y
Cuts at: 0 173 1536 2491 2730
Size: 173 1363 955 239
Fragments arranged by size:
1363 955 239 173
BstZ17I GTA'TAC
Cuts at: 0 158 1103 1283 1319 1355 1391 1427 2730
Size: 158 945 180 36 36 36 36 1303
Fragments arranged by size:
1303 945 180 158 36 36 36 36
Bsu36I CC'TnA_GG
Cuts at: 0 402 2730
Size: 402 2328
BtgI C'CryG_G
Cuts at: 0 934 1706 2331 2671 2730
Size: 934 772 625 340 59
Fragments arranged by size:
934 772 625 340 59
BtsI GCAGTG_nn'
Cuts at: 0 1797 2730
Size: 1797 933
EaeI y'GGCC_r
Cuts at: 0 183 656 2568 2730
Size: 183 473 1912 162
Fragments arranged by size:
1912 473 183 162
EarI CTCTTCn'nnn_
Cuts at: 0 674 848 2117 2730
Size: 674 174 1269 613
Fragments arranged by size:
1269 674 613 174
EcoICRI GAG'CTC
Cuts at: 0 585 2730
Size: 585 2145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 487 1277 1706 2227 2539 2604 2730
Size: 146 341 790 429 521 312 65 126
Fragments arranged by size:
790 521 429 341 312 146 126 65
EcoO109I rG'GnC_Cy
Cuts at: 0 1072 1180 1252 1396 1432 1681 2730
Size: 1072 108 72 144 36 249 1049
Fragments arranged by size:
1072 1049 249 144 108 72 36
EcoRV GAT'ATC
Cuts at: 0 335 2730
Size: 335 2395
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1846 2730
Size: 1846 884
FspI TGC'GCA
Cuts at: 0 2231 2730
Size: 2231 499
HaeII r_GCGC'y
Cuts at: 0 2079 2730
Size: 2079 651
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 796 828 849 881 991 1023 1027 1059
Size: 796 32 21 32 110 32 4 32
Cuts at: 1059 1459 1491 1528 1560 1868 1900 2483 2515
Size: 400 32 37 32 308 32 583 32
Cuts at: 2515 2730
Size: 215
Fragments arranged by size:
796 583 400 308 215 110 37 32
32 32 32 32 32 32 32 21
4
HincII GTy'rAC
Cuts at: 0 2358 2730
Size: 2358 372
HindIII A'AGCT_T
Cuts at: 0 720 2153 2422 2730
Size: 720 1433 269 308
Fragments arranged by size:
1433 720 308 269
MscI TGG'CCA
Cuts at: 0 185 2730
Size: 185 2545
MslI CAynn'nnrTG
Cuts at: 0 48 610 1675 2730
Size: 48 562 1065 1055
Fragments arranged by size:
1065 1055 562 48
MspA1I CmG'CkG
Cuts at: 0 241 1881 1998 2313 2333 2730
Size: 241 1640 117 315 20 397
Fragments arranged by size:
1640 397 315 241 117 20
NcoI C'CATG_G
Cuts at: 0 934 1706 2730
Size: 934 772 1024
Fragments arranged by size:
1024 934 772
NdeI CA'TA_TG
Cuts at: 0 1528 2730
Size: 1528 1202
NheI G'CTAG_C
Cuts at: 0 408 2730
Size: 408 2322
NspI r_CATG'y
Cuts at: 0 714 2221 2369 2730
Size: 714 1507 148 361
Fragments arranged by size:
1507 714 361 148
PfoI T'CCnGG_A
Cuts at: 0 102 337 2730
Size: 102 235 2393
Fragments arranged by size:
2393 235 102
PmlI CAC'GTG
Cuts at: 0 1172 2730
Size: 1172 1558
PshAI GACnn'nnGTC
Cuts at: 0 560 2730
Size: 560 2170
PsiI TTA'TAA
Cuts at: 0 893 2730
Size: 893 1837
PstI C_TGCA'G
Cuts at: 0 622 2003 2132 2730
Size: 622 1381 129 598
Fragments arranged by size:
1381 622 598 129
PvuII CAG'CTG
Cuts at: 0 241 2313 2730
Size: 241 2072 417
Fragments arranged by size:
2072 417 241
SacI G_AGCT'C
Cuts at: 0 587 2730
Size: 587 2143
SacII CC_GC'GG
Cuts at: 0 2334 2730
Size: 2334 396
SapI GCTCTTCn'nnn_
Cuts at: 0 2117 2730
Size: 2117 613
ScaI AGT'ACT
Cuts at: 0 2440 2730
Size: 2440 290
SexAI A'CCwGG_T
Cuts at: 0 1769 2730
Size: 1769 961
SfcI C'TryA_G
Cuts at: 0 618 1598 1999 2128 2278 2730
Size: 618 980 401 129 150 452
Fragments arranged by size:
980 618 452 401 150 129
SmlI C'TyrA_G
Cuts at: 0 316 2115 2730
Size: 316 1799 615
Fragments arranged by size:
1799 615 316
SphI G_CATG'C
Cuts at: 0 2221 2730
Size: 2221 509
SspI AAT'ATT
Cuts at: 0 2039 2730
Size: 2039 691
StuI AGG'CCT
Cuts at: 0 1840 1855 2236 2539 2730
Size: 1840 15 381 303 191
Fragments arranged by size:
1840 381 303 191 15
StyI C'CwwG_G
Cuts at: 0 684 934 1706 2247 2730
Size: 684 250 772 541 483
Fragments arranged by size:
772 684 541 483 250
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 291 2730
Size: 291 2439
TatI w'GTAC_w
Cuts at: 0 52 1066 1174 1246 1495 1531 1567 1639
Size: 52 1014 108 72 249 36 36 72
Cuts at: 1639 1775 2438 2730
Size: 136 663 292
Fragments arranged by size:
1014 663 292 249 136 108 72 72
52 36 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 165 197 442 474 1736 1768 2373 2405
Size: 165 32 245 32 1262 32 605 32
Cuts at: 2405 2730
Size: 325
Fragments arranged by size:
1262 605 325 245 165 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 607 1987 2730
Size: 607 1380 743
Fragments arranged by size:
1380 743 607
XmnI GAAnn'nnTTC
Cuts at: 0 1019 2730
Size: 1019 1711
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlfI AlwNI
ApoI BanI BanII BbsI BciVI BglI BglII BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaHI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrGI BssSI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI
BtsI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV FalI
FspI HaeII Hin4I HincII HindIII MscI MslI MspA1I
NcoI NdeI NheI NspI PfoI PmlI PshAI PsiI
PstI PvuII SacI SacII SapI ScaI SexAI SfcI
SmlI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AloI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbeI BbvCI BcgI BclI BfrBI BlpI Bme1580I Bpu10I
BsaBI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BsrBI
BsrDI BsrFI BssHII BstBI BstEII BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoNI EcoRI FseI
FspAI HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NgoMIV NotI NruI NsiI PacI PasI PciI
PflMI PmeI PpiI PpuMI PspOMI PspXI PsrI PvuI
RsrII SalI SanDI SbfI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SwaI XbaI XcmI XhoI
XmaI ZraI