(Circular) (Six-base) MAPSORT of: pFN21AA1826.seq Check: 3017 from: 1 to: 5740 pFN21AA1826 5740 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2396 2396 Size: 5740 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3550 278 Size: 53 83 186 936 2014 2468 Fragments arranged by size: 2468 2014 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3083 1937 Size: 1146 4594 Acc65I G'GTAC_C Cuts at: 2220 3061 2220 Size: 841 4899 AclI AA'CG_TT Cuts at: 1563 3867 4240 1563 Size: 2304 373 3063 Fragments arranged by size: 3063 2304 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2349 2738 3803 5187 5455 1632 Size: 717 389 1065 1384 268 1917 Fragments arranged by size: 1917 1384 1065 717 389 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5543 AflIII A'CryG_T Cuts at: 1203 4639 5616 1203 Size: 3436 977 1327 Fragments arranged by size: 3436 1327 977 AgeI A'CCGG_T Cuts at: 4571 4571 Size: 5740 AhdI GACnn_n'nnGTC Cuts at: 4469 4469 Size: 5740 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5740 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5740 AlwNI CAG_nnn'CTG Cuts at: 3483 5055 3483 Size: 1572 4168 ApaLI G'TGCA_C Cuts at: 3797 4953 3797 Size: 1156 4584 ApoI r'AATT_y Cuts at: 1493 2368 2465 3015 3049 3275 3427 5536 1493 Size: 875 97 550 34 226 152 2109 1697 Fragments arranged by size: 2109 1697 875 550 226 152 97 34 AseI AT'TA_AT Cuts at: 160 1032 2124 4294 160 Size: 872 1092 2170 1606 Fragments arranged by size: 2170 1606 1092 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 AvaI C'yCGr_G Cuts at: 1870 1943 3065 1870 Size: 73 1122 4545 Fragments arranged by size: 4545 1122 73 BamHI G'GATC_C Cuts at: 2411 2449 3070 3437 2411 Size: 38 621 367 4714 Fragments arranged by size: 4714 621 367 38 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2220 3061 4516 618 Size: 325 217 627 433 841 1455 1842 Fragments arranged by size: 1842 1455 841 627 433 325 217 BanII G_rGCy'C Cuts at: 1391 3059 1391 Size: 1668 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4769 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3931 3965 1903 Size: 34 1994 34 3678 Fragments arranged by size: 3678 1994 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3632 4842 3632 Size: 1210 4530 BclI T'GATC_A Cuts at: 1548 1548 Size: 5740 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4351 136 Size: 107 122 71 631 605 2679 1525 Fragments arranged by size: 2679 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2588 5735 2588 Size: 3147 2593 BlpI GC'TnA_GC Cuts at: 3157 3157 Size: 5740 Bme1580I G_kGCm'C Cuts at: 1790 3801 4957 1790 Size: 2011 1156 2573 Fragments arranged by size: 2573 2011 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5740 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3471 4429 442 Size: 416 53 83 399 24 2054 958 1753 Fragments arranged by size: 2054 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5740 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4400 1239 Size: 3161 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2924 3865 4730 5028 2924 Size: 941 865 298 3636 Fragments arranged by size: 3636 941 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4403 882 Size: 617 2904 2219 Fragments arranged by size: 2904 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3531 493 Size: 711 2327 2702 Fragments arranged by size: 2702 2327 711 BsaBI GATnn'nnATC Cuts at: 5734 5734 Size: 5740 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3547 3929 4564 275 Size: 53 83 186 936 2014 382 635 1451 Fragments arranged by size: 2014 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4173 4571 4845 4992 5403 4173 Size: 398 274 147 411 4510 Fragments arranged by size: 4510 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5515 Fragments arranged by size: 5515 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2293 2492 2495 1186 Size: 1107 199 3 4431 Fragments arranged by size: 4431 1107 199 3 BseYI C'CCAG_C Cuts at: 4943 5439 4943 Size: 496 5244 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5167 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3190 3951 4979 1540 Size: 275 186 1189 761 1028 2301 Fragments arranged by size: 2301 1189 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2239 3801 3886 4957 2239 Size: 1562 85 1071 3022 Fragments arranged by size: 3022 1562 1071 85 BsmI GAATG_Cn' Cuts at: 2176 3251 3344 2176 Size: 1075 93 4572 Fragments arranged by size: 4572 1075 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2239 3059 3801 3886 4957 1391 Size: 399 449 820 742 85 1071 2174 Fragments arranged by size: 2174 1071 820 742 449 399 85 BspEI T'CCGG_A Cuts at: 5403 5403 Size: 5740 BspHI T'CATG_A Cuts at: 3629 5359 3629 Size: 1730 4010 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2396 2674 3028 3095 844 Size: 1552 278 354 67 3489 Fragments arranged by size: 3489 1552 354 278 67 BsrBI CCG'CTC Cuts at: 1394 1823 2749 2770 3627 1394 Size: 429 926 21 857 3507 Fragments arranged by size: 3507 926 857 429 21 BsrDI GCAATG_nn' Cuts at: 65 1248 4235 4409 65 Size: 1183 2987 174 1396 Fragments arranged by size: 2987 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4384 4571 1700 Size: 110 2574 187 2869 Fragments arranged by size: 2869 2574 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5740 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5740 BssSI C'ACGA_G Cuts at: 3800 4812 3800 Size: 1012 4728 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5740 BstBI TT'CG_AA Cuts at: 3013 4544 3013 Size: 1531 4209 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5740 BstYI r'GATC_y Cuts at: 1972 2411 2449 2588 3070 3437 3822 3839 5291 Size: 439 38 139 482 367 385 17 1452 Cuts at: 5291 5377 5389 5735 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 482 439 385 367 346 139 86 38 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5188 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5001 Fragments arranged by size: 5001 683 56 BtsI GCAGTG_nn' Cuts at: 3256 4050 4070 3256 Size: 794 20 4926 Fragments arranged by size: 4926 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5740 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5705 DraI TTT'AAA Cuts at: 3044 3044 Size: 5740 DraIII CAC_nnn'GTG Cuts at: 2165 2165 Size: 5740 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4747 809 Size: 507 3431 1802 Fragments arranged by size: 3431 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3187 4076 5705 Size: 54 1425 50 275 18 1357 889 1629 Cuts at: 5705 8 Size: 43 Fragments arranged by size: 1629 1425 1357 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3187 1537 Size: 275 1375 4090 Fragments arranged by size: 4090 1375 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2145 2240 2325 2430 3670 1353 Size: 792 95 85 105 1240 3423 Fragments arranged by size: 3423 1240 792 105 95 85 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4327 4701 4847 387 Size: 1064 138 2738 374 146 1280 Fragments arranged by size: 2738 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2349 2738 3803 4400 5187 5455 1239 Size: 393 717 389 1065 597 787 268 1524 Fragments arranged by size: 1524 1065 787 717 597 393 389 268 EcoRI G'AATT_C Cuts at: 3015 3049 3427 3015 Size: 34 378 5328 Fragments arranged by size: 5328 378 34 EcoRV GAT'ATC Cuts at: 2665 2665 Size: 5740 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3004 3026 3004 Size: 22 5718 FspI TGC'GCA Cuts at: 4246 4246 Size: 5740 HaeII r_GCGC'y Cuts at: 1678 4887 1678 Size: 3209 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2649 2681 2810 2842 4381 Size: 32 135 32 1077 32 129 32 1539 Cuts at: 4381 4413 4455 4487 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1539 1077 135 129 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3084 3330 669 Size: 988 281 1146 246 3079 Fragments arranged by size: 3079 1146 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5740 HpaI GTT'AAC Cuts at: 3330 3330 Size: 5740 KpnI G_GTAC'C Cuts at: 2224 3065 2224 Size: 841 4899 MfeI C'AATT_G Cuts at: 3339 3339 Size: 5740 MluI A'CGCG_T Cuts at: 5616 5616 Size: 5740 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2625 4854 5038 1305 Size: 1320 2229 184 2007 Fragments arranged by size: 2229 2007 1320 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3918 Fragments arranged by size: 3918 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2553 3698 4057 4216 518 Size: 648 1387 1145 359 159 2042 Fragments arranged by size: 2042 1387 1145 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3156 3833 4981 5226 1606 Size: 1550 677 1148 245 2120 Fragments arranged by size: 2120 1550 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5188 NdeI CA'TA_TG Cuts at: 387 2596 387 Size: 2209 3531 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5740 NotI GC'GGCC_GC Cuts at: 3187 3187 Size: 5740 NspI r_CATG'y Cuts at: 1132 1229 3098 4643 1132 Size: 97 1869 1545 2229 Fragments arranged by size: 2229 1869 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5740 PciI A'CATG_T Cuts at: 4639 4639 Size: 5740 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5740 PfoI T'CCnGG_A Cuts at: 2516 2516 Size: 5740 PmeI GTTT'AAAC Cuts at: 3044 3044 Size: 5740 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3809 3841 5322 5354 3809 Size: 32 1481 32 4195 Fragments arranged by size: 4195 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5740 PsiI TTA'TAA Cuts at: 3310 3310 Size: 5740 PstI C_TGCA'G Cuts at: 830 2685 2778 2943 2973 3092 830 Size: 1855 93 165 30 119 3478 Fragments arranged by size: 3478 1855 165 119 93 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5740 SalI G'TCGA_C Cuts at: 1936 3082 1936 Size: 1146 4594 SbfI CC_TGCA'GG Cuts at: 3092 3092 Size: 5740 ScaI AGT'ACT Cuts at: 3988 5700 3988 Size: 1712 4028 SfcI C'TryA_G Cuts at: 826 1045 2175 2681 2774 2939 2969 3088 4223 Size: 219 1130 506 93 165 30 119 1135 Cuts at: 4223 4904 5095 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1130 681 506 219 191 165 119 93 30 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5740 SmaI CCC'GGG Cuts at: 1872 3067 1872 Size: 1195 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2903 3844 4745 5007 820 Size: 197 926 960 941 901 262 1553 Fragments arranged by size: 1553 960 941 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3531 493 Size: 3038 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5740 SphI G_CATG'C Cuts at: 1132 3098 1132 Size: 1966 3774 SspI AAT'ATT Cuts at: 5 52 2623 3664 5 Size: 47 2571 1041 2081 Fragments arranged by size: 2571 2081 1041 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5740 StyI C'CwwG_G Cuts at: 513 1065 2829 3179 513 Size: 552 1764 350 3074 Fragments arranged by size: 3074 1764 552 350 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3767 3784 3937 4122 4567 325 Size: 1255 2187 17 153 185 445 1498 Fragments arranged by size: 2187 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3986 5698 96 Size: 274 80 33 51 1443 2009 1712 138 Fragments arranged by size: 2009 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5700 5732 5700 Size: 32 5708 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5740 XbaI T'CTAG_A Cuts at: 2585 3076 2585 Size: 491 5249 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2272 2272 Size: 5740 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5740 XmaI C'CCGG_G Cuts at: 1870 3065 1870 Size: 1195 4545 XmnI GAAnn'nnTTC Cuts at: 3869 3869 Size: 5740 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3548 276 Size: 53 83 186 936 2014 2468 Fragments arranged by size: 2468 2014 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI