Restriction map for
pFN21AA1803
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# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AhdI AocI Aor51HI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BfrI BglI BlnI BsaBI
BseAI BsiWI Bsp1407I BspDI BspEI
BsrGI BssHII Bsu36I ClaI CpoI
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoRV FseI FspI KpnI KspI
MamI MfeI MluI MroI MunI
NcoI NotI NruI PacI PflMI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI TfiI Van91I XmaCI
XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccII 2779 CGCG CG^CG
AflIII 4011 ACATGT A^C[GA][CT]GT
AgeI 1265 ACCGGT A^CCGGT
Asp700I 3369 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2707 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2765 TGGCCA TGG^CCA
BbrPI 3430 CACGTG CAC^GTG
BciVI 2686 GGATAC GTATCC(6/5)
BclI 1763 TGATCA T^GATCA
BglII 3474 AGATCT A^GATCT
BlpI 1800 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1800 GCTGAGC GC^T[ACGT]AGC
BsaAI 3430 CACGTG [CT]AC^GT[GA]
BsgI 3541 CTGCAC GTGCAG(16/14)
BsmBI 1228 GAGACG CGTCTC(1/5)
BspHI 2742 TCATGA T^CATGA
BspLU11I 4011 ACATGT A^CATGT
BsrBI 301 GAGCGG CCGCTC(-3/-3)
BssSI 1545 CACGAG CACGAG(-5/-1)
Bst1107I 830 GTATAC GTA^TAC
BstEII 1161 GGTGACC G^GT[ACGT]ACC
BstPI 1161 GGTGACC G^GT[ACGT]ACC
BstUI 2779 CGCG CG^CG
BstZ17I 830 GTATAC GTA^TAC
CelII 1800 GCTGAGC GC^T[ACGT]AGC
DraI 3409 TTTAAA TTT^AAA
EcoO65I 1161 GGTGACC G^GT[ACGT]ACC
EcoT22I 2125 ATGCAT ATGCA^T
FbaI 1763 TGATCA T^GATCA
HincII 3805 GTTAAC GT[CT]^[GA]AC
HindII 3805 GTTAAC GT[CT]^[GA]AC
HindIII 3370 AAGCTT A^AGCTT
HpaI 3805 GTTAAC GTT^AAC
MluNI 2765 TGGCCA TGG^CCA
MscI 2765 TGGCCA TGG^CCA
MvnI 2779 CGCG CG^CG
NaeI 2918 GCCGGC GCC^GGC
NdeI 2845 CATATG CA^TATG
NgoMIV 2918 GCCGGC G^CCGGC
NheI 906 GCTAGC G^CTAGC
NsiI 2125 ATGCAT ATGCA^T
PaeR7I 1390 CTCGAG C^TCGAG
PflFI 2707 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1265 ACCGGT A^CCGGT
PmaCI 3430 CACGTG CAC^GTG
PmlI 3430 CACGTG CAC^GTG
PpuMI 2150 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 1145 GACACCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2742 TCATGA T^CATGA
SacI 3846 GAGCTC GAGCT^C
SapI 3550 GAAGAGC GCTCTTC(1/4)
ScaI 1532 AGTACT AGT^ACT
SexAI 1493 ACCTGGT A^CC[AT]GGT
StuI 2380 AGGCCT AGG^CCT
Tth111I 2707 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 444 TCTAGA T^CTAGA
XcmI 2606 CCAGTGGCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1390 CTCGAG C^TCGAG
XmnI 3369 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 830 GTATAC GT^[AC][GT]AC
1075 GTCTAC
ApaI 19 GGGCCC GGGCC^C
3465 GGGCCC
BbeI 1258 GGCGCC GGCGC^C
2611 GGCGCC
BbsI 3133 GAAGAC GAAGAC(2/6)
3898 GAAGAC
BbvCI 1795 CCTCAGC CCTCAGC(-5/-2)
1168 GCTGAGG
BfaI 445 CTAG C^TAG
907 CTAG
BpuAI 3133 GAAGAC GAAGAC(2/6)
3898 GAAGAC
BsaI 3724 GAGACC GGTCTC(1/5)
1497 GGTCTC
BsmI 4215 GAATGC GAATGC(1/-1)
703 GCATTC
BspMI 3640 ACCTGC ACCTGC(4/8)
4050 GCAGGT
BstBI 233 TTCGAA TT^CGAA
661 TTCGAA
BstXI 1934 CCAGCAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1988 CCACACTGATGG
BstYI 1238 GGATCT [GA]^GATC[CT]
3474 AGATCT
DraIII 1504 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
3532 CACATCGTG
EagI 1958 CGGCCG C^GGCCG
2677 CGGCCG
EclXI 1958 CGGCCG C^GGCCG
2677 CGGCCG
Eco52I 1958 CGGCCG C^GGCCG
2677 CGGCCG
EcoT14I 24 CCAAGG C^C[AT][AT]GG
1781 CCAAGG
HgaI 2602 GACGC GACGC(5/10)
3339 GCGTC
KasI 1258 GGCGCC G^GCGCC
2611 GGCGCC
MaeI 445 CTAG C^TAG
907 CTAG
MflI 1238 GGATCT [GA]^GATC[CT]
3474 AGATCT
NarI 1258 GGCGCC GG^CGCC
2611 GGCGCC
NspV 233 TTCGAA TT^CGAA
661 TTCGAA
PstI 901 CTGCAG CTGCA^G
2108 CTGCAG
SfoI 1258 GGCGCC GGC^GCC
2611 GGCGCC
SfuI 233 TTCGAA TT^CGAA
661 TTCGAA
SmlI 1390 CTCGAG C^T[CT][GA]AG
3025 CTCAAG
SphI 2123 GCATGC GCATG^C
2958 GCATGC
StyI 24 CCAAGG C^C[AT][AT]GG
1781 CCAAGG
XhoII 1238 GGATCT [GA]^GATC[CT]
3474 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
AspHI AvaI AvaII BanI BanII
BbvI BcgI BcnI BmyI BpmI
BsaHI BsaJI BsaWI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I