(Circular) (Six-base) MAPSORT of: pFN21AA1803.seq Check: 7806 from: 1 to: 8941 pFN21AA1803 8941 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5654 6046 5654 Size: 392 8549 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6751 278 Size: 53 83 186 936 5215 2468 Fragments arranged by size: 5215 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2836 3081 6284 1937 Size: 899 245 3203 4594 Fragments arranged by size: 4594 3203 899 245 Acc65I G'GTAC_C Cuts at: 6262 6262 Size: 8941 AclI AA'CG_TT Cuts at: 1563 7068 7441 1563 Size: 5505 373 3063 Fragments arranged by size: 5505 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2901 3435 5036 5148 5304 5922 7004 8388 Size: 1269 534 1601 112 156 618 1082 1384 Cuts at: 8388 8656 1632 Size: 268 1917 Fragments arranged by size: 1917 1601 1384 1269 1082 618 534 268 156 112 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8744 AflIII A'CryG_T Cuts at: 1203 6016 7840 8817 1203 Size: 4813 1824 977 1327 Fragments arranged by size: 4813 1824 1327 977 AgeI A'CCGG_T Cuts at: 3270 7772 3270 Size: 4502 4439 AhdI GACnn_n'nnGTC Cuts at: 7670 7670 Size: 8941 AleI CACnn'nnGTG Cuts at: 1166 3416 1166 Size: 2250 6691 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4365 1184 Size: 3181 5760 AlwNI CAG_nnn'CTG Cuts at: 2658 2816 4544 4738 6684 8256 2658 Size: 158 1728 194 1946 1572 3343 Fragments arranged by size: 3343 1946 1728 1572 194 158 ApaI G_GGCC'C Cuts at: 2028 5474 2028 Size: 3446 5495 ApaLI G'TGCA_C Cuts at: 2953 3185 4466 6998 8154 2953 Size: 232 1281 2532 1156 3740 Fragments arranged by size: 3740 2532 1281 1156 232 ApoI r'AATT_y Cuts at: 1493 3350 4289 4451 5303 5312 5972 6250 6476 Size: 1857 939 162 852 9 660 278 226 Cuts at: 6476 6628 8737 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1857 1697 939 852 660 278 226 162 152 9 AseI AT'TA_AT Cuts at: 160 1032 7495 160 Size: 872 6463 1606 Fragments arranged by size: 6463 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8941 AvaI C'yCGr_G Cuts at: 1870 1943 3395 3401 5462 6266 1870 Size: 73 1452 6 2061 804 4545 Fragments arranged by size: 4545 2061 1452 804 73 6 BamHI G'GATC_C Cuts at: 6271 6638 6271 Size: 367 8574 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3074 3263 3522 4408 4616 Size: 325 217 627 1287 189 259 886 208 Cuts at: 4616 6262 7717 618 Size: 1646 1455 1842 Fragments arranged by size: 1842 1646 1455 1287 886 627 325 259 217 208 189 BanII G_rGCy'C Cuts at: 1391 2028 2511 3402 3425 3468 3604 5169 5474 Size: 637 483 891 23 43 136 1565 305 Cuts at: 5474 5855 6002 6260 1391 Size: 381 147 258 4072 Fragments arranged by size: 4072 1565 891 637 483 381 305 258 147 136 43 23 BbeI G_GCGC'C Cuts at: 3267 4620 3267 Size: 1353 7588 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5145 5910 928 Size: 971 3246 765 3959 Fragments arranged by size: 3959 3246 971 765 BbvCI CC'TCA_GC Cuts at: 3174 3801 3174 Size: 627 8314 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2229 2263 3689 3723 4470 4504 7132 Size: 34 292 34 1426 34 747 34 2628 Cuts at: 7132 7166 1903 Size: 34 3678 Fragments arranged by size: 3678 2628 1426 747 292 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4685 6833 8043 4685 Size: 2148 1210 5583 Fragments arranged by size: 5583 2148 1210 BclI T'GATC_A Cuts at: 1548 3768 1548 Size: 2220 6721 BfrBI ATG'CAT Cuts at: 4132 4132 Size: 8941 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7552 136 Size: 107 122 71 631 605 5880 1525 Fragments arranged by size: 5880 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5479 8936 5479 Size: 3457 5484 BlpI GC'TnA_GC Cuts at: 3806 6358 3806 Size: 2552 6389 Bme1580I G_kGCm'C Cuts at: 1790 2028 2957 3189 3640 4470 5451 5474 7002 Size: 238 929 232 451 830 981 23 1528 Cuts at: 7002 8158 1790 Size: 1156 2573 Fragments arranged by size: 2573 1528 1156 981 929 830 451 238 232 23 BmgBI CAC'GTC Cuts at: 1318 2427 3226 4510 1318 Size: 1109 799 1284 5749 Fragments arranged by size: 5749 1284 1109 799 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3361 6672 7630 Size: 416 53 83 399 24 1944 3311 958 Cuts at: 7630 442 Size: 1753 Fragments arranged by size: 3311 1944 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2915 1056 Size: 1859 7082 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4279 4279 Size: 8941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2324 3721 5635 5651 5839 6236 7601 1239 Size: 1085 1397 1914 16 188 397 1365 2579 Fragments arranged by size: 2579 1914 1397 1365 1085 397 188 16 Bpu10I CC'TnA_GC Cuts at: 3174 3801 4053 3174 Size: 627 252 8062 Fragments arranged by size: 8062 627 252 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5015 7066 7931 8229 5015 Size: 2051 865 298 5727 Fragments arranged by size: 5727 2051 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3508 5723 7604 882 Size: 617 2009 2215 1881 2219 Fragments arranged by size: 2219 2215 2009 1881 617 BsaAI yAC'GTr Cuts at: 493 1204 5437 6732 493 Size: 711 4233 1295 2702 Fragments arranged by size: 4233 2702 1295 711 BsaBI GATnn'nnATC Cuts at: 8935 8935 Size: 8941 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3264 4608 4617 5344 Size: 53 83 186 936 1731 1344 9 727 Cuts at: 5344 6748 7130 7765 275 Size: 1404 382 635 1451 Fragments arranged by size: 1731 1451 1404 1344 936 727 635 382 186 83 53 9 BsaWI w'CCGG_w Cuts at: 2145 3270 3519 7374 7772 8046 8193 8604 2145 Size: 1125 249 3855 398 274 147 411 2482 Fragments arranged by size: 3855 2482 1125 411 398 274 249 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3455 3485 3488 3518 3530 Size: 30 165 30 2052 30 3 30 12 Cuts at: 3530 3560 5090 5120 1178 Size: 30 1530 30 4999 Fragments arranged by size: 4999 2052 1530 165 30 30 30 30 30 30 12 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3245 3461 3464 3476 4025 4333 4768 5072 Size: 2059 216 3 12 549 308 435 304 Cuts at: 5072 5077 5095 5308 5480 1186 Size: 5 18 213 172 4647 Fragments arranged by size: 4647 2059 549 435 308 304 216 213 172 18 12 5 3 BseYI C'CCAG_C Cuts at: 2591 3603 4528 5444 8144 8640 2591 Size: 1012 925 916 2700 496 2892 Fragments arranged by size: 2892 2700 1012 925 916 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5531 1163 Size: 573 3795 4573 Fragments arranged by size: 4573 3795 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2254 3966 4685 6391 7152 8180 Size: 275 186 253 1712 719 1706 761 1028 Cuts at: 8180 1540 Size: 2301 Fragments arranged by size: 2301 1712 1706 1028 761 719 275 253 186 BsiHKAI G_wGCw'C Cuts at: 2332 2351 2957 3189 3848 3857 4470 4988 5855 Size: 19 606 232 659 9 613 518 867 Cuts at: 5855 7002 7087 8158 2332 Size: 1147 85 1071 3115 Fragments arranged by size: 3115 1147 1071 867 659 613 606 518 232 85 19 9 BsmI GAATG_Cn' Cuts at: 2708 6226 6452 6545 2708 Size: 3518 226 93 5104 Fragments arranged by size: 5104 3518 226 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3227 3227 Size: 8941 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2332 2351 2511 2957 3189 3402 Size: 399 238 304 19 160 446 232 213 Cuts at: 3402 3425 3468 3604 3640 3848 3857 4470 4988 Size: 23 43 136 36 208 9 613 518 Cuts at: 4988 5169 5451 5474 5855 6002 6260 7002 7087 Size: 181 282 23 381 147 258 742 85 Cuts at: 7087 8158 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 613 518 446 399 381 304 282 258 238 232 213 208 181 160 147 136 85 43 36 23 23 19 9 BspEI T'CCGG_A Cuts at: 8604 8604 Size: 8941 BspHI T'CATG_A Cuts at: 4747 6830 8560 4747 Size: 2083 1730 5128 Fragments arranged by size: 5128 2083 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5654 6046 6296 844 Size: 4810 392 250 3489 Fragments arranged by size: 4810 3489 392 250 BsrBI CCG'CTC Cuts at: 1394 1823 2308 6828 1394 Size: 429 485 4520 3507 Fragments arranged by size: 4520 3507 485 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3094 4528 4696 7436 7610 65 Size: 1183 1846 1434 168 2740 174 1396 Fragments arranged by size: 2740 1846 1434 1396 1183 174 168 BsrFI r'CCGG_y Cuts at: 1700 1810 3270 4923 5583 7585 7772 1700 Size: 110 1460 1653 660 2002 187 2869 Fragments arranged by size: 2869 2002 1653 1460 660 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8941 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8941 BssSI C'ACGA_G Cuts at: 3550 7001 8013 3550 Size: 3451 1012 4478 Fragments arranged by size: 4478 3451 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2961 3096 4089 4358 4738 4896 5394 794 Size: 2167 135 993 269 380 158 498 4341 Fragments arranged by size: 4341 2167 993 498 380 269 158 135 BstBI TT'CG_AA Cuts at: 2239 2667 7745 2239 Size: 428 5078 3435 Fragments arranged by size: 5078 3435 428 BstEII G'GTnAC_C Cuts at: 1184 3166 1184 Size: 1982 6959 BstXI CCAn_nnnn'nTGG Cuts at: 3946 4000 3946 Size: 54 8887 BstYI r'GATC_y Cuts at: 1972 3243 5479 6271 6638 7023 7040 8492 8578 Size: 1271 2236 792 367 385 17 1452 86 Cuts at: 8578 8590 8936 1972 Size: 12 346 1977 Fragments arranged by size: 2236 1977 1452 1271 792 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2837 2837 Size: 8941 BtgI C'CryG_G Cuts at: 513 1065 3121 4864 5038 5066 5932 513 Size: 552 2056 1743 174 28 866 3522 Fragments arranged by size: 3522 2056 1743 866 552 174 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2452 5877 487 Size: 683 56 1226 3425 3551 Fragments arranged by size: 3551 3425 1226 683 56 BtsI GCAGTG_nn' Cuts at: 2351 2476 3721 3834 4110 4645 5296 6457 7251 Size: 125 1245 113 276 535 651 1161 794 Cuts at: 7251 7271 2351 Size: 20 4021 Fragments arranged by size: 4021 1245 1161 794 651 535 276 125 113 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5347 5382 529 Size: 35 4783 35 4088 Fragments arranged by size: 4783 4088 35 35 DraI TTT'AAA Cuts at: 5416 6245 5416 Size: 829 8112 DraIII CAC_nnn'GTG Cuts at: 3514 5542 3514 Size: 2028 6913 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7948 809 Size: 507 6632 1802 Fragments arranged by size: 6632 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2042 2229 3963 Size: 54 1425 50 275 18 212 187 1734 Cuts at: 3963 4682 4770 6388 7277 8906 8 Size: 719 88 1618 889 1629 43 Fragments arranged by size: 1734 1629 1618 1425 889 719 275 212 187 88 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3963 4682 6388 1537 Size: 275 2151 719 1706 4090 Fragments arranged by size: 4090 2151 1706 719 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2796 3632 4002 4398 5550 6871 1353 Size: 1443 836 370 396 1152 1321 3423 Fragments arranged by size: 3423 1443 1321 1152 836 396 370 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4574 7528 7902 8048 387 Size: 1064 138 2985 2954 374 146 1280 Fragments arranged by size: 2985 2954 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5853 5853 Size: 8941 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2324 2901 3435 3721 5036 5148 5304 Size: 393 692 577 534 286 1315 112 156 Cuts at: 5304 5635 5651 5839 5922 6236 7004 7601 8388 Size: 331 16 188 83 314 768 597 787 Cuts at: 8388 8656 1239 Size: 268 1524 Fragments arranged by size: 1524 1315 787 768 692 597 577 534 393 331 314 286 268 188 156 112 83 16 EcoO109I rG'GnC_Cy Cuts at: 2024 2025 2813 4156 5470 5471 2024 Size: 1 788 1343 1314 1 5494 Fragments arranged by size: 5494 1343 1314 788 1 1 EcoRI G'AATT_C Cuts at: 4289 5303 5312 6250 6628 4289 Size: 1014 9 938 378 6602 Fragments arranged by size: 6602 1014 938 378 9 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3865 4120 4186 5309 5656 3865 Size: 255 66 1123 347 7150 Fragments arranged by size: 7150 1123 347 255 66 FspI TGC'GCA Cuts at: 7447 7447 Size: 8941 HaeII r_GCGC'y Cuts at: 1678 3267 3697 4620 8088 1678 Size: 1589 430 923 3468 2531 Fragments arranged by size: 3468 2531 1589 923 430 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3455 3487 4878 4910 5062 Size: 32 135 32 1883 32 1391 32 152 Cuts at: 5062 5094 6057 6089 7582 7614 7656 7688 1373 Size: 32 963 32 1493 32 42 32 2626 Fragments arranged by size: 2626 1883 1493 1391 963 152 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5812 6285 6531 669 Size: 988 281 3874 473 246 3079 Fragments arranged by size: 3874 3079 988 473 281 246 HindIII A'AGCT_T Cuts at: 748 5375 748 Size: 4627 4314 HpaI GTT'AAC Cuts at: 5812 6531 5812 Size: 719 8222 KasI G'GCGC_C Cuts at: 3263 4616 3263 Size: 1353 7588 KpnI G_GTAC'C Cuts at: 6266 6266 Size: 8941 MfeI C'AATT_G Cuts at: 6540 6540 Size: 8941 MluI A'CGCG_T Cuts at: 8817 8817 Size: 8941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4647 5782 8055 8239 1305 Size: 3342 1135 2273 184 2007 Fragments arranged by size: 3342 2273 2007 1135 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4772 10 Size: 54 1425 343 2940 4179 Fragments arranged by size: 4179 2940 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2335 2570 2950 3029 3164 3416 3591 Size: 648 1169 235 380 79 135 252 175 Cuts at: 3591 3998 4885 4956 6899 7258 7417 518 Size: 407 887 71 1943 359 159 2042 Fragments arranged by size: 2042 1943 1169 887 648 407 380 359 252 235 175 159 135 79 71 MspA1I CmG'CkG Cuts at: 1606 2065 2277 3173 3805 4080 4532 4541 4800 Size: 459 212 896 632 275 452 9 259 Cuts at: 4800 5014 5207 5625 5824 6357 7034 8182 8427 Size: 214 193 418 199 533 677 1148 245 Cuts at: 8427 1606 Size: 2120 Fragments arranged by size: 2120 1148 896 677 632 533 459 452 418 275 259 245 214 212 199 193 9 NaeI GCC'GGC Cuts at: 4925 4925 Size: 8941 NarI GG'CG_CC Cuts at: 3264 4617 3264 Size: 1353 7588 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 8389 NdeI CA'TA_TG Cuts at: 387 4851 387 Size: 4464 4477 NgoMIV G'CCGG_C Cuts at: 4923 4923 Size: 8941 NheI G'CTAG_C Cuts at: 1052 2911 1052 Size: 1859 7082 NotI GC'GGCC_GC Cuts at: 6388 6388 Size: 8941 NsiI A_TGCA'T Cuts at: 4134 4134 Size: 8941 NspI r_CATG'y Cuts at: 1132 1229 4132 4967 6020 6299 7844 1132 Size: 97 2903 835 1053 279 1545 2229 Fragments arranged by size: 2903 2229 1545 1053 835 279 97 PasI CC'CwG_GG Cuts at: 1348 2718 3911 1348 Size: 1370 1193 6378 Fragments arranged by size: 6378 1370 1193 PciI A'CATG_T Cuts at: 6016 7840 6016 Size: 1824 7117 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8941 PfoI T'CCnGG_A Cuts at: 2850 3737 4581 6213 2850 Size: 887 844 1632 5578 Fragments arranged by size: 5578 1632 887 844 PmeI GTTT'AAAC Cuts at: 6245 6245 Size: 8941 PmlI CAC'GTG Cuts at: 5437 5437 Size: 8941 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5013 5045 7010 7042 8523 8555 5013 Size: 32 1965 32 1481 32 5399 Fragments arranged by size: 5399 1965 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4156 4156 Size: 8941 PshAI GACnn'nnGTC Cuts at: 1613 3154 1613 Size: 1541 7400 PsiI TTA'TAA Cuts at: 6511 6511 Size: 8941 PspOMI G'GGCC_C Cuts at: 2024 5470 2024 Size: 3446 5495 PspXI vC'TCGA_Gb Cuts at: 3395 3395 Size: 8941 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4196 4228 4196 Size: 32 8909 PstI C_TGCA'G Cuts at: 830 2910 4117 6293 830 Size: 2080 1207 2176 3478 Fragments arranged by size: 3478 2176 2080 1207 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8941 PvuII CAG'CTG Cuts at: 2065 2277 3805 4532 4541 4800 5014 5207 5824 Size: 212 1528 727 9 259 214 193 617 Cuts at: 5824 2065 Size: 5182 Fragments arranged by size: 5182 1528 727 617 259 214 212 193 9 SacI G_AGCT'C Cuts at: 5855 5855 Size: 8941 SalI G'TCGA_C Cuts at: 1936 6283 1936 Size: 4347 4594 SapI GCTCTTCn'nnn_ Cuts at: 5550 5550 Size: 8941 SbfI CC_TGCA'GG Cuts at: 6293 6293 Size: 8941 ScaI AGT'ACT Cuts at: 3539 7189 8901 3539 Size: 3650 1712 3579 Fragments arranged by size: 3650 3579 1712 SexAI A'CCwGG_T Cuts at: 3498 3498 Size: 8941 SfcI C'TryA_G Cuts at: 826 1045 2644 2906 4113 6289 7424 8105 8296 Size: 219 1599 262 1207 2176 1135 681 191 Cuts at: 8296 826 Size: 1471 Fragments arranged by size: 2176 1599 1471 1207 1135 681 262 219 191 SfoI GGC'GCC Cuts at: 3265 4618 3265 Size: 1353 7588 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8941 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8941 SmaI CCC'GGG Cuts at: 1872 6268 1872 Size: 4396 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3395 5030 7045 7946 8208 820 Size: 197 926 1452 1635 2015 901 262 1553 Fragments arranged by size: 2015 1635 1553 1452 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6732 493 Size: 6239 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8941 SphI G_CATG'C Cuts at: 1132 4132 4967 6299 1132 Size: 3000 835 1332 3774 Fragments arranged by size: 3774 3000 1332 835 SspI AAT'ATT Cuts at: 5 52 6865 5 Size: 47 6813 2081 Fragments arranged by size: 6813 2081 47 StuI AGG'CCT Cuts at: 1517 4387 1517 Size: 2870 6071 StyI C'CwwG_G Cuts at: 513 1065 2029 3786 6380 513 Size: 552 964 1757 2594 3074 Fragments arranged by size: 3074 2594 1757 964 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2268 3924 4485 6093 6968 6985 7138 Size: 1255 688 1656 561 1608 875 17 153 Cuts at: 7138 7323 7768 325 Size: 185 445 1498 Fragments arranged by size: 1656 1608 1498 1255 875 688 561 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3537 4794 7187 Size: 274 80 33 51 1443 1560 1257 2393 Cuts at: 7187 8899 96 Size: 1712 138 Fragments arranged by size: 2393 1712 1560 1443 1257 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3488 3520 4049 4081 5090 5122 8901 8933 3488 Size: 32 529 32 1009 32 3779 32 3496 Fragments arranged by size: 3779 3496 1009 529 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4715 1316 Size: 3399 5542 XbaI T'CTAG_A Cuts at: 2449 6277 2449 Size: 3828 5113 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4618 4618 Size: 8941 XhoI C'TCGA_G Cuts at: 1943 3395 1943 Size: 1452 7489 XmaI C'CCGG_G Cuts at: 1870 6266 1870 Size: 4396 4545 XmnI GAAnn'nnTTC Cuts at: 5378 7070 5378 Size: 1692 7249 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6749 276 Size: 53 83 186 936 5215 2468 Fragments arranged by size: 5215 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BsiWI Bsu36I EcoNI EcoRV FseI FspAI NruI PacI RsrII SacII SanDI SfiI SrfI SwaI